JP2537572B2 - Automatic analyzer measurement method - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a measuring method for an automatic analyzer, and more particularly, in an automatic analyzer used for a clinical test, when the automatic analyzer in a stopped state requires highly urgent analysis. In addition, the present invention relates to a measuring method of an automatic analyzer which shortens the time from the start of operation to the pipetting of a sample.

[0002]

2. Description of the Related Art Conventional automatic analyzers include the techniques disclosed in, for example, Japanese Patent Publication No. 2-35811, Japanese Patent Laid-Open No. 64-69936, and Japanese Patent Laid-Open No. 61-40569. In the continuous analysis method disclosed in Japanese Examined Patent Publication (Kokoku) No. 2-32581, a large number of reaction vessels are provided in a reaction vessel table, and when the reaction vessels containing a reaction solution composed of a sample and a reagent are sequentially measured, each reaction The cell blank value for the container can be determined, and the measured value of the reaction solution can be efficiently corrected. Specifically, on the reaction container table, prepare a reaction container containing the final reaction liquid and a reaction container containing the blank liquid, transfer these reaction containers, perform photometry together, and measure the final reaction liquid. Values and blank measurements were allowed to be obtained simultaneously in a common cycle. Therefore, by correlating the reaction solution measurement value in a certain reaction vessel with the blank measurement value of the same reaction vessel obtained in the previous cycle, the reaction solution measurement value of the final reaction is efficiently corrected one after another. It becomes possible. In the cell blank processing method disclosed in Japanese Patent Application Laid-Open No. 64-69936, it is possible to perform 1 day a day before starting analysis.
In view of the problem of the conventional measurement method of measuring and storing a cell blank once, the cell blank value is determined by a predetermined method each time analysis is performed in consideration of the drift variation of the actual cell blank. Specifically, before starting the photometry, the cell blank value is measured twice, and the cell blank value adopted in the previous measurement and the two cell blank values of this time are arranged in the order of the value, and the intermediate value is set. It is configured to adopt the value as the cell blank value. This makes the reliability of the cell blank high. JP-A-61-405
In the automatic analyzer disclosed in Japanese Patent Publication No. 69, reaction vessels for reagent blank solution and reaction vessels for reaction solution are alternately formed, and photometry is sequentially performed, and absorbance values are sequentially stored. Then, the average value of the reagent blank values measured before and after the reaction solution is subtracted from the measured value of the reaction solution, and correction is performed to improve the measurement accuracy.

[0003]

At present, automatic analyzers are required to be able to quickly perform measurement / analysis when an emergency inspection is required. In an automatic analyzer of the type in which cell blank measurement is performed for each reaction container before photometry / analysis is started, conventionally, when the analysis request information for inspection was input to this device, the analysis information was input. After that, it was necessary to wait for the completion of a series of processes for measuring the cell blank value of the reaction container necessary for cleaning and analysis of the reaction container until the sample was actually sampled and the reaction was started. This waiting time was actually about 2 minutes, but it was a serious delay in an emergency examination in a clinical examination. Here, a series of operations from the cleaning of the reaction container to the measurement of the cell blank value is called “pretreatment”. In order to eliminate the waiting time until the completion of this pretreatment in an emergency test, the pretreatment should be performed in advance in the automatic analyzer, and when the execution command for the emergency test is input, the sampling of the sample should be performed immediately. It is desirable to be able to do so. Therefore, the present applicant previously applied for a measurement method for emergency inspection (Japanese Patent Application No. 2-111411).
issue). However, the measurement method for such an emergency inspection requires further improvement in the following points. In the automatic analyzer,
When the test is stopped so that the test can be performed, the sample can be immediately dispensed to the reaction container at the sample pipetting position when the test is requested. . As for the reaction vessel at the sample pipetting position, the cell blank value is already measured before the automatic analyzer is stopped, so that the cell blank value can be used immediately. However, after the cell blank measurement of the reaction vessel, while the automatic analyzer is stopped, because of drift due to changes in the optical system, changes in the light source, etc. Blank values are less reliable. For urgent tests, it is most desirable to measure using a reaction vessel at the sample pipetting position from the viewpoint of speed, but the cell blank value of the reaction vessel is corrected and its reliability is improved. It is necessary to raise it. The three known techniques described in the section "Prior Art" all propose a method for correcting the amount of subsequent drift of the already measured cell blank value of the reaction vessel used in the emergency test as described above. Moreover, even if these publicly known documents are combined, the method cannot be implied.

The object of the invention is to carry out the analysis before the analysis.
By performing pretreatment that can obtain cell blank values of all wavelengths required for various analyses, in the measuring method of the automatic analyzer that can cope with emergency inspection, when the automatic analyzer is in the stopped state, It is an object of the present invention to provide a measuring method for an automatic analyzer, which intends to quickly respond to an emergency test by using a reliable cell blank value when the emergency test is requested.

[0005]

Means for Solving the Problems The measuring method of an automatic analyzer according to the present invention is such that a liquid is put into a reaction container used for the analysis before the analysis, and then photometry is carried out.
All the cell blank values required for the analysis of the reaction container are measured, and any of the cell blank values is a measuring method of an automatic analyzer that is used at the time of analysis, and the automatic analyzer is in a stopped state from the state described above. When starting the analysis, set the cell blank value of the reaction vessel existing at the position where the sample is pipetted to the latest cell blank value measured in the past and the other cell blank value existing at the position where the cell blank can be measured. The reaction container is characterized in that it is obtained on the basis of the degree of change between the cell blank value of this time, which was actually measured, and the cell blank value of the previous time. In the measuring method of the automatic analyzer,
When the cell blank measurement is performed for each reaction container before the automatic analyzer is stopped, the latest cell blank value is stored.

[0006]

In the measuring method of the automatic analyzer according to the present invention, the cell blank values of all the wavelengths required for various subsequent analyzes are measured for each reaction container at the pretreatment stage before the analysis. , Configured to remember this,
Especially when the automatic analyzer is in a stopped state, an urgent inspection is required, and when the operator performs analysis request information and start of the inspection, the automatic analyzer will detect the reaction vessels existing at the sample pipetting position. , Cell blank measurement is not performed at the time of operation start, cell blank measurement is performed for other reaction vessels existing at the position where cell blank measurement can be performed, and for this other reaction vessel, the cell blank value measured this time and the past measurement The degree of change from the latest previous cell blank value obtained in step 1 is obtained as the amount of drift of the photometric system, and with this amount of drift, the latest cell previously stored for the reaction vessel present at the pipetting position. Correct the blank value, and for the reaction container used for emergency inspection, obtain the cell blank value for emergency inspection without performing cell blank measurement. It is configured so that. As a result, the reaction container existing at the sample pipetting position can be immediately used to quickly perform an emergency test. A special operation mode is provided for each reaction container so that the latest cell blank value is stored.

[0007]

EXAMPLES Examples of the present invention will be described below with reference to FIGS.
It will be described based on. FIG. 1 is a schematic configuration diagram of an automatic analyzer, and FIG. 2 is an enlarged view of a part of a reaction disk and shows a positional relationship between a main part of a cell blank measuring mechanism and a reaction container. In FIG. 1, reference numeral 1 is a reaction disk, and the reaction disk 1 is rotatably attached. The automatic analyzer is equipped with a rotary drive mechanism for rotating the reaction disk 1, but its illustration is omitted. A large number of reaction vessels 2, for example 120, are provided on the outer circumference of the reaction disk 1. The whole reaction disk 1 is kept at a predetermined temperature by a heat retaining tank 3. 4 is
It is a constant temperature bath. 5 is a sample sample disk mechanism,
The mechanism 5 is provided with a large number of sample cups 6. The sample in the sample cup 6 is appropriately extracted by the nozzle 8 of the sample pipetting mechanism 7 and injected into a predetermined reaction container. Reference numeral 9 is a reagent disk mechanism provided with a large number of reagent cups. Each reagent disk mechanism 9 has
Reagent pipetting mechanisms 10A and 10B are provided. 11 arranged close to the reagent disk mechanism 9
Is a stirring mechanism. Reference numeral 12 is a multi-wavelength photometer, 13 is a light source, and the reaction container 2 for accommodating the object of photometry is arranged between the multi-wavelength photometer 12 and the light source 13. 14 is a cleaning mechanism. Regarding the control system and the signal processing system, 15 is a microcomputer, 16 is an interface, 17 is a Log (logarithmic) converter, 18 is an A / D converter, 19 is a reagent dispensing mechanism, 20 is a wash water pump, 21 is a reagent. It is a dispensing mechanism. Further, 22 is a printer, 23 is a CRT, 24 is a floppy disk as a storage device, and 25 is an operation panel. Although not shown in FIG. 1, the cleaning mechanism 14
The cell blank measuring mechanism section described with reference to FIG. 2 is disposed between the sample sample disk mechanism 5 and the sample sample disk mechanism 5.

In the above structure, the operator who operates the automatic analyzer to perform the analysis inputs the analysis request information using the operation panel 25. The input analysis request information is stored in the memory in the microcomputer 15. The sample placed in the sample cup 6 and set at a predetermined position on the sample disk 5 is the microcomputer 15
According to the analysis request information stored in the memory of the sample pipetting mechanism 21 by the nozzle 8 of the sample pipetting mechanism 7.
Based on the action of, a predetermined amount is dispensed into the reaction container 2. 12
The reaction disk 1 in which 0 reaction vessels 2 are installed is
One machine cycle (20 seconds) and one reaction container (12
Rotate one). In the reaction container 2 into which the sample has been dispensed, among the reagent cups arranged on the reagent disc 9, a predetermined reagent cup is selected according to the stored analysis request information, and the nozzles of the reagent pipetting mechanisms 10A and 10B are selected. A predetermined amount is dispensed by using the action of the reagent dispensing mechanism 19. Then, the stirring mechanism 11 stirs and mixes the sample and the reagent. The sample in the reaction container is kept at a constant temperature by circulating water supplied from the constant temperature bath 4, and is reacted for a predetermined time. After the reaction, the absorbance is measured in the multi-wavelength photometer 12 using the set two wavelengths. The photometric absorbance is taken into the microcomputer 15 via the multiplexer, the Log converter 17, the A / D converter 18, and the interface 16. The absorbed absorbance is corrected by the cell blank value and then converted into a concentration, which is stored in the floppy disk 24 or is output to the printer 22 as a result. The inspection data can also be displayed on the CRT 23. The reaction container whose measurement has been completed is washed once in each machine cycle (20 seconds) by using the ion exchange water sent by the washing water pump 20 in the washing mechanism 14. The ion-exchanged water for cleaning added by the cleaning mechanism 14 is discharged by a vacuum pump (not shown) every time cleaning is performed. After repeating this washing operation three times in about 1 minute, a part of the ion-exchanged water added last is left in the reaction vessel and used for the cell blank measurement in the next stage.

The cell blank measuring method according to the present invention will be described in detail with reference to FIG. In FIG. 2, the reaction disk 1
The numbers 1 to 120 written in the squares on the outer peripheral part of the are the numbers attached to the reaction vessels, that is, the cell numbers. Also, the number written inside the cell number is the cell position number. The arrow 30 indicates the rotation direction of the reaction disk 1. Here, a measurement method for obtaining a cell blank value when an emergency inspection is performed from a state in which the automatic analyzer is stopped will be described.

Now, assume that the automatic analyzer is stopped in the position shown in FIG. When an emergency inspection is requested, the sample aspirated by the nozzle 8 of the sample pipetting mechanism is
It is dispensed into the reaction container of cell number 101 in the cell position 101. At the same time, the cleaning mechanism 14 starts operating, and the reaction disk 1 makes one revolution and one revolution in one machine cycle.
The rotation operation for the reaction container is started. Therefore, the cell number of the reaction container is shifted in the direction of arrow 30 by one reaction container in one machine cycle. The reaction container with the cell number 101 located at the cell position number 101 is 1
After the machine cycle, it moves to the position of cell position number 100. The reaction container of the cell number 1 is cleaned by the cleaning nozzle of the cleaning mechanism 14, and further, after cleaning is performed by the cleaning nozzles of the cleaning mechanism 14 every two machine cycles, ion-exchanged water is injected by the nozzle. It After one machine cycle, the reaction container of cell number 1 stops at the position of cell position number 112 for a predetermined time, and at this time, the photometric axis 31 generated from the light source 13 passes through the reaction container at cell position number 112. The light that has passed through the reaction container is separated by the spectroscopic grading 32. The separated light of each wavelength is measured by, for example, each of 12 light receiving elements arranged in the detector 33, input to the microcomputer 15, and stored in the memory thereof. The reaction container stopped on the photometric axis 31 of the cell blank measuring mechanism changes its position sequentially for every one cell position after each cell blank measurement after the completion of the measurement of the cell blank. Ion-exchanged water for cell blank measurement is sucked and discharged by the suction nozzles 34A and 34B at the position. As described above, the cell blank measurement can be performed on cells having cell numbers 1 and later. Therefore, for the cells of cell number 1 and later, using the cell blank value obtained in the cell blank measurement after the suspended automatic analyzer starts its operation for the emergency inspection,
It is possible to obtain the amount of drift from the previous cell blank value measured by the automatic analyzer before the stop. On the other hand, the reaction vessels of cell numbers 101 to 120 are
Cell blank measurements cannot be taken after the automated analyzer has been activated. Therefore, the method for obtaining the cell blank value for the reaction vessels 101 to 120 into which the sample is dispensed without measuring the cell blank is as follows. In addition,
The reaction vessels of cell numbers 101 to 120 that are not subjected to cell blank measurement are referred to as cells to be corrected, and a plurality of reaction vessels after cell number 1 capable of measuring cell blanks, for example, 6 reaction vessels are referred to as correction cells. For the correction cell and the cell to be corrected, before the automatic analyzer is stopped, and before entering the state of emergency inspection, perform cell blank measurement in advance,
For each reaction container, the cell blank values for all wavelengths required for various analyzes are stored in the memory as the previous cell blank values.

When an operation for an emergency inspection is started from a state in which the automatic analyzer is stopped, cell blank values of all wavelengths of the correction cells are measured by performing cell blank measurements, and cell numbers 1 to 1 are obtained. Obtained sequentially for each of the 6 reaction vessels. For each reaction container, the difference between the current cell blank value and the previously measured previous cell blank value is calculated, and the average of the reaction containers of cell numbers 1 to 6 is calculated for this difference. The average value of this difference is treated as the amount of drift of the photometric system while the automatic analyzer was stopped. Then, the cell blank value of the corrected cell that has been subjected to analysis without cell blank measurement, with respect to the previous cell blank value that has been measured in advance, the difference obtained as a drift amount by the correction cell Determined by adding. The following is the formula for calculating the drift amount and the formula for calculating the cell blank value of the cell to be corrected this time.

[0012]

[Equation 1]

[0013]

[Equation 2] (cell to be corrected, current cell blank value) _j = (cell to be corrected, previous cell blank value) _j + drift amount As described above, the current cell blank value of each reaction container of the cell to be corrected Then, photometry and analysis can be immediately performed. Note that the above-described method of obtaining the drift amount is an example, and it is not necessary to obtain the average value, for example.

[0014]

As is apparent from the above description, according to the present invention, in the automatic analyzer, when the emergency inspection is performed after the apparatus is stopped, the present cell blank value of the reaction container that can be used earliest in the emergency inspection. Therefore, without performing the cell blank measurement, the cell blank value that was previously performed before the device was stopped and the drift amount obtained by the cell blank value of the correction cell that can be measured this time cell blank were obtained. By using the reaction container that can be used as soon as possible for analysis, it is possible to reduce the time between actual analysis start and highly accurate analysis in case of an emergency analysis request when the automatic analyzer is stopped. It can be carried out.

[Brief description of drawings]

FIG. 1 is a schematic configuration diagram of an automatic analyzer.

FIG. 2 is a view showing a part of a reaction disk and a cell blank mechanism section.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Reaction disk 2 Reaction container 5 Sample sample disk mechanism 9 Reagent disk mechanism 11 Stirring mechanism 12 Multi-wavelength photometer 13 Light source 14 Cleaning mechanism 15 Microcomputer 25 Operation panel 31 Photometric axis 32 Spectral grading 33 Detector

Claims (2)

(57) [Claims] 1. Before performing an analysis, a liquid is put into a reaction container used for the analysis, and then photometry is performed to measure all cell blank values required for the analysis for the reaction container. A method for measuring an automatic analyzer that uses any of the cell blank values during analysis, in the case of starting the analysis from a state in which the automatic analyzer is stopped, a reaction container existing at a position for pipetting a sample. For the cell blank value of the latest cell blank value that was measured in the past and other reaction vessels existing at the position where the cell blank can be measured, the cell blank value of A measuring method for an automatic analyzer, characterized in that it is obtained by correcting based on a cell blank value and a degree of change between the values. 2. The method for measuring an automatic analyzer according to claim 1, wherein when the cell blank measurement is performed for each reaction container before the automatic analyzer is stopped, the latest cell blank value is obtained. A method for measuring an automatic analyzer characterized by being stored.