JPH10267935A - Cuvette correction method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To improve reliability of stored data, by obtaining stored data to be used for correcting measured values of a specimen to be inspected which are caused by contamination of a reaction vessel, by averaging a plurality of data. SOLUTION: An automatic analysis equipment of a reaction vessel direct photometry system cleans a plurality of reaction vessels (serving as measuring vessels) 2 arranged on a reaction table 1 and uses them again. When the reaction vessels 2 are new or clean, the cuvette blank value is measured and stored about each reaction vessel, in the state that water is contained in the vessel, before analysis is started. At the time of cleaning, in the course of analysis, in the state that cleaning water is contained in the reaction vessel 2, cleaning liquid measured values are sequentially obtained and stored about each reaction vessel. The average value of a plurality of data containing the cuvette blank value and the latest cleaning liquid measured value is obtained at each wavelength and about each reaction vessel, and sequentially updated and stored. The stored data concerning said reaction vessel and said wavelength which are used for measuring the specimen to be inspected are extracted from the average value. On the basis of stored data, the measured value of the specimen to be inspected is corrected.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for correcting a cuvette in an automatic analyzer.

[0002]

2. Description of the Related Art Automatic analyzers for determining the concentrations of various components in blood are widely used in clinical tests. In such an automatic analyzer, when the reaction vessel direct photometry method in which the reaction vessel is also used as a measurement vessel is employed, the amount of the test solution can be reduced by a small amount as compared with the case where a method in which a test sample is transferred to another measurement vessel is employed. This is advantageous because it becomes possible to form As the amount of the test solution is reduced, first, the amount of the sample used is reduced, thereby reducing the burden on the patient. Second, the amount of the reagent used is reduced, so that the running cost is reduced. Furthermore, by washing and repeatedly using the reaction vessel,
The reaction container direct photometry method has been widely used because the number of consumables is reduced and the running cost of the inspection is greatly reduced. However, since the reaction vessel is used repeatedly as described above, how to reduce the influence of contamination of the reaction vessel becomes an issue.

In order to solve this problem, various prior arts have been proposed. For example, Japanese Patent Publication No. 61-24653 discloses that after cleaning a reaction vessel, before repeated use, a cleanliness test is performed in a state in which water is contained in the reaction vessel, and the state of dirt is determined by the magnitude of absorption. There has been proposed a sample analysis method and apparatus for controlling a reaction vessel in which a contamination not less than a predetermined value is detected, and is not used for analysis. In addition, Japanese Patent Publication No. 1-51786 discloses that a dirt in a reaction vessel is optically measured while washing water is put in the reaction vessel.
There has been proposed a technique for correcting a measured value obtained by performing a measurement in a state where a reaction solution (a test sample) is placed in the same reaction container, based on the obtained measured value.

[0004]

SUMMARY OF THE INVENTION The above Japanese Patent Publication No. Sho 61-24
In the prior art of JP-A No. 653/1995, the state of contamination of a reaction vessel repeatedly used after washing is detected, and when the contamination of a predetermined value or more is detected, the use of the reaction vessel is stopped. Soil that has occurred immediately before the determination to stop is not greatly affected by the measurement result, but the absorption due to the stain causes an error in the measurement result. In addition, since the dirt on the reaction vessel gradually accumulates, the number of reaction vessels that cannot be used increases with the elapse of time, and the processing capacity for analysis is significantly reduced.

On the other hand, in the prior art disclosed in Japanese Patent Publication No. 1-51786, a measured value in a state in which a reaction solution (a sample to be tested) is put is used by using a measured value in a state in which a washing solution in the same reaction vessel is put. The value is corrected, but in this case,
The following problem arises because the measured value itself with the cleaning liquid in it is used for correction. That is, since the measured value always contains an error, if the measured value of the test sample is corrected by using only one measurement result as a reference value, it is affected by a random error at the time of photometry, and the This leads to a reduction in the reliability of the correction value itself used for the purpose of reducing the influence of dirt.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problem, and it is an object of the present invention to measure contamination of a reaction vessel and to correct data used for correction when correcting a measured value of a test sample based on the measured value. The purpose is to improve reliability.

[0007]

To achieve the above object, a method according to claim 1 of the present invention includes a plurality of reaction vessels, and the plurality of reaction vessels are washed and used repeatedly and also used as a measurement vessel. In a reaction vessel direct photometric automatic analyzer for measuring a sample to be measured, if the reaction vessel is new or clean, before starting the analysis, the cuvette blank value is set in a state in which the reaction vessel is filled with water. Measuring and storing for each reaction vessel, and when cleaning the reaction vessel during the analysis operation, sequentially measuring and storing the measured value of the washing liquid for each reaction vessel with the washing water in the reaction vessel. A step of obtaining an average value of a plurality of data including the cuvette blank value and the latest measured value of the cleaning liquid for each of the reaction vessels for each of the wavelengths, sequentially updating and storing the average value, and using the average value to measure a test sample. Were extracted stored data related to the corresponding wavelength of the corresponding reaction vessel, characterized in that it consists of a step of correcting the measured value of the test sample on the basis of the stored data.

In the method of the present invention, in order to reduce the influence of the contamination of the reaction vessel on the measured value of the test sample, and to improve the reliability of data used for correction corresponding to the contamination of the reaction vessel used, Instead of using one data, the average value of multiple data is used.

That is, in the method according to the first aspect of the present invention, as data used for correction for reducing the influence of contamination of the reaction vessel, an average value obtained by averaging a plurality of data for each wavelength of the same reaction vessel. Use The calculation method of the average value is as follows. First, if all of the plurality of reaction vessels are new or clean, prior to the start of analysis, cuvette blank measurement was performed for each reaction vessel with water in all of the reaction vessels, and the resulting cuvette blank was measured. Store the value.

Next, when the reaction vessel is washed during the analysis operation (when the washing position is reached), the measurement of the cuvette washing liquid is sequentially performed for each reaction vessel in a state where the washing water is filled in the reaction vessel. The measured value of the cleaning liquid is stored. Next, for each reaction vessel, an average value of a plurality of data including a cuvette blank value and the latest measured value of the cleaning liquid is obtained for each of the wavelengths, and sequentially updated and stored. Next, from among the average values stored for each wavelength for each reaction vessel, the storage data for the corresponding wavelength of the reaction vessel corresponding to the one used for the measurement containing the test sample is extracted, and the stored data is The measured value of the test sample is corrected based on the measured value.

[0011] To this end, the method according to claim 2 of the present invention comprises a plurality of reaction vessels, and the plurality of reaction vessels are washed and used repeatedly and also used as a measurement vessel to measure a test sample. In a reaction vessel direct photometric automatic analyzer for performing the measurement, if the reaction vessel is new or clean, before starting the analysis, in a state where the reaction vessel is filled with water, a wavelength that may be used for measurement. Measuring and storing the cuvette blank value for each of the reaction vessels for each of the reaction vessels, and at the time of washing the reaction vessels during the analysis operation, there is a possibility that the reaction vessels may be used for the measurement with the washing water put therein. A step of sequentially measuring and storing the measured values of the cleaning solution for each of the wavelengths for each reaction container, and obtaining and writing a difference value between the measured value of the cleaning solution and the cuvette blank value for each of the wavelengths for each reaction container. A step of obtaining an average value of the difference values for each of the reaction vessels for each of the wavelengths and sequentially updating and storing the data, and storing the storage data relating to the corresponding wavelength of the corresponding reaction vessel used for measuring the test sample from the average value. Extracting and correcting the measured value of the test sample based on the stored data.

In the method according to the second aspect of the present invention, an average value obtained by averaging a plurality of data for each wavelength of the same reaction vessel is used as data used for correction for reducing the influence of contamination of the reaction vessel. I do. The calculation method of the average value is as follows. First, if all of the plurality of reaction vessels are new or clean, before starting the analysis, with all the reaction vessels filled with water, for each of the wavelengths that may be used for measurement, one for each reaction vessel. A cuvette blank measurement is performed and the resulting cuvette blank value is stored.

Next, when the reaction vessel is washed during the analysis operation (at the time of reaching the washing position), the reaction vessel is filled with the washing water, and each of the wavelengths that may be used for the measurement is set for the reaction vessel. The cuvette cleaning liquid measurement is sequentially performed every time, and the obtained cleaning liquid measurement value is stored. Next, for each reaction vessel,
For each of the wavelengths, a difference value between the cleaning liquid measurement value and the cuvette blank value is obtained and stored, and an average value of the difference values obtained so far is obtained and sequentially updated and stored. Next, from among the average values stored for each wavelength for each reaction vessel, the storage data for the corresponding wavelength of the reaction vessel corresponding to the one used for the measurement containing the test sample is extracted, and the stored data is The measured value of the test sample is corrected based on the measured value.

To achieve the above object, a method according to a third aspect of the present invention includes a plurality of reaction vessels, and the plurality of reaction vessels are washed and used repeatedly, and at the same time, are used as a measurement vessel to measure a test sample. In a reaction vessel direct photometric automatic analyzer for performing the measurement, if the reaction vessel is new or clean, before starting the analysis, in a state where the reaction vessel is filled with water, a wavelength that may be used for measurement. Measuring and storing the cuvette blank value for each of the reaction vessels for each of the reaction vessels, and at the time of washing the reaction vessels during the analysis operation, there is a possibility that the reaction vessels may be used for the measurement with the washing water put therein. A step of sequentially measuring and storing the cleaning liquid measurement value for each of the wavelengths for each reaction container, and obtaining a difference value between a previous cleaning liquid measurement value and the cuvette blank value for each of the wavelengths for each reaction container. Calculating and sequentially updating and storing an average value of difference values for a plurality of reaction vessels including a reaction vessel corresponding to the latest storage data for each of the wavelengths, and measuring a test sample from the average value. Extracting the stored data relating to the corresponding wavelength used in step (a), and correcting the measured value of the test sample based on the stored data.

In the method according to the third aspect of the present invention, an average value obtained by averaging data on each wavelength of all reaction vessels is used as data used for correction for reducing the influence of contamination of the reaction vessels. . The calculation method of the average value is as follows. First, if all of the plurality of reaction vessels are new or clean, before starting the analysis, with all the reaction vessels filled with water, for each of the wavelengths that may be used for measurement, one for each reaction vessel. A cuvette blank measurement is performed and the resulting cuvette blank value is stored.

Next, when the reaction vessel is washed during the analysis operation (when the cleaning position is reached), the reaction vessel is filled with the washing water, and the reaction vessel is subjected to each of the wavelengths that may be used for measurement. The cuvette cleaning liquid measurement is sequentially performed every time, and the obtained cleaning liquid measurement value is stored. Next, for each of the wavelengths, a difference value between a previous cleaning liquid measurement value and the cuvette blank value is obtained and stored for each reaction container, and a difference value for a plurality of reaction containers including a reaction container corresponding to the latest storage data is obtained. The average of the values is determined and sequentially updated and stored.
Next, from the average value stored for each wavelength, storage data relating to the wavelength corresponding to the measured test sample is extracted, and the measured value of the test sample is corrected based on the stored data.

[0017]

Embodiments of the present invention will be described below in detail with reference to the drawings. FIG. 1 is a diagram illustrating the configuration of an automatic analyzer used for performing the cuvette correction method according to the first embodiment of the present invention. The automatic analyzer used in the present embodiment is a general-purpose automatic analyzer generally used widely. As shown in FIG. 1, a plurality of reaction / measurement containers (reaction container and A container used as a measurement container, hereinafter, referred to as a cuvette) 2 is provided.

The reaction table 1 is rotated stepwise by an angle of moving one cuvette in the counterclockwise direction, and while the reaction table 1 is rotated one turn + 1 cuvette in the counterclockwise direction, all the cuvettes 2 will be described later. Each operation is performed at each position. That is, first, at the dispensing position S1 shown in FIG. 1, a sample collected from the sample cup 4 on the sampler 3 by a dispenser (not shown) is dispensed into the cuvette 2 in a fixed amount. The cuvette 2 reaches the dispensing position S2 and stops when the reaction table 1 rotates (one round + 1 cuvette) after dispensing a fixed amount. Dispensing position S2
Then, a reagent collected from a reagent container 6 on the first reagent table 5 by a dispenser (not shown) is dispensed into the cuvette 2 in a fixed amount.

Thereafter, the reaction table 1 is repeatedly rotated and stopped, and the reaction vessel row is moved stepwise in the counterclockwise direction. The reagent contained in the reagent container 6 is dispensed. The stop positions S3 and S5 after dispensing the reagent are respectively
A stirring device 8 is provided, and the test sample is mixed by the stirring device 8.

Thereafter, the sample and the reagent dispensed into the cuvette 2 react and become a test sample. When the cuvette 2 containing the test sample crosses the photometric light beam 9 from the photometric device (not shown) installed at the measurement position S6 by rotating the reaction table 1, the transmittance or the absorbance is measured at multiple wavelengths. After that, the cuvette 2 for which the measurement has been completed is
After the test sample is discharged by a discharge device (not shown) at a discharge position S7, the test sample is washed by a cleaning device (not shown) at a cleaning position S8, and is drained and dried by a drainage device (not shown) at a drainage drying position S9. Provided for reuse.

While the above series of operations is repeated, the cuvette 2 is washed each time the measurement is completed, but dirt gradually accumulates in the cuvette 2 due to repeated use. Focusing on this, in the present embodiment, in order to reduce the influence of the stain on the measured value, the stain on the cuvette is measured, and the measurement result of the test sample is corrected based on the measured value.

Specifically, before starting the analysis, the analyzer is operated in advance, and all the cuvettes placed on the reaction table 1 are set as new or washed cuvettes 2, and in this state, water is stored in the cuvettes 2. And a cuvette blank value is obtained for each cuvette for each wavelength that may be used for measurement and stored. During the analysis,
At the cleaning position S8, the cuvette cleaning liquid measurement value (hereinafter, referred to as the cleaning liquid measurement value) is obtained for each of the wavelengths that may be used for the measurement with the cleaning water in the cuvette 2;
Remember. Then, for each wavelength for each reaction vessel,
The average value of the cuvette blank value and the measured value of the cleaning liquid including at least the previous value is obtained, sequentially updated and stored. From the average value obtained for each wavelength for each reaction vessel, the test sample is stored and used for measurement. The stored data relating to the corresponding wavelength of the reaction vessel corresponding to the extracted data is extracted, and the measured value of the test sample is corrected based on the stored data.

In the statistical processing of data, a method of using an average value of a plurality of data by increasing the number of data samplings is generally used to reduce the influence of random errors. In this case, the degree of reduction in the influence of the random error is 1 / √
It is said to be N. In the present embodiment, a method of calculating stored data will be described by taking an example where N = 4.

First, in the first (first round) analysis after the measurement of the cuvette blank value, there is no cleaning solution measurement value that can be used, so that the measurement value is corrected using only the cuvette blank value. As the cuvette blank value, for example, as shown in Table 1, when the number of cuvettes 2 arranged in the reaction table 1 is N and wavelengths that may be used for measurement are λ ₁ , λ _{2 to} λ _{n , a 011, a 02 1,} ···, and the data string of a _0N1, a _012, a ₀₂₂ corresponding to the wavelength λ ₂ ~λ _n corresponding to the wavelength λ _1, ··
·, Data column ~a _01n of _{a 0N2, a 02n, ···,} a
A data string of _0Nn is obtained. A corresponding one is extracted from these data strings and used as correction data.

[0025]

[Table 1]

In the second lap, since the measured value of the cleaning liquid is obtained in the measurement of the first lap, the measured value is corrected using the average value of the cuvette blank value and the measured value of the cleaning liquid in the first lap. As shown in Table 2, for example, as shown in Table 2, a ₁₁₁ and a a corresponding to the wavelength λ _1
121, ..., and a data row of a _1N1, a _112, a _122, corresponding to the wavelength lambda ₂ to [lambda] _n, ..., data strings of a _1N2 ~
Data strings of a _11n , a _12n ,..., a _1Nn are obtained. Here, for example, the cuvette is # 01 and the wavelength is λ.
The average value a _{v11 in} the case of ₁ is a _v11 = (a ₀₁₁ + a
₁₁₁ ) / 2, and the average value a _vNn when the cuvette is #N and the wavelength is λ _n is a _vNn = (a _0Nn + a _1Nn ) /
It becomes 2. The average value a _vNn thus obtained is used as correction data.

[0027]

[Table 2]

In the third lap, the measured value is corrected using the average value of the cuvette blank value and the measured value of the cleaning liquid in the first and second laps. As shown in Table 3, for example, as shown in Table 3, _a211 and a2 corresponding to the wavelength λ _1
221, ..., and a data row of a _2N1, a _212, a _222, corresponding to the wavelength lambda ₂ to [lambda] _n, ..., data strings of a _2N2 ~
a _21n, a _22n, ···, a data string of a _2Nn obtained. The average value a _vNn when the cuvette is #N and the wavelength is λ _n is a _vNn = (a _0Nn + a _1Nn + a _2Nn ) /
It becomes 3. The average value a _vNn thus obtained is used as correction data.

[0029]

[Table 3]

On the fourth lap, the above cuvette blank value and
Measured using the average value with the measured values of the cleaning liquid in the first, second and third laps
Correct the fixed value. In this case, the cuvette is #N
Length is λ _nAverage value a in the case of_vNnIs a_vNn= (A_0Nn+
a_1Nn+ A_2Nn+ A_3Nn) / 4. Like this
Average value a_vNnIs used as correction data. Less than
In the same way, consider the accumulation of dirt due to repeated use
Corrected data is obtained, and this corrected data is
Multiplied by the measured value and the ratio of the measured value
Highly measured values are obtained.

In the case where the number of times the reaction vessel is repeatedly used (for example, 5 times or more) before the completion of all the measurements to be analyzed, the old measured value of the washing liquid is used for correction from the next time. It is also possible to average a predetermined number of measured values of the cleaning liquid from the latest one so as not to use. In this case as well, the initial blank value is desirably left as a reference value, but may not be used for simplicity. When the blank value is not used, the cuvette blank value and each measured value of the washing liquid are added and averaged immediately after the start of the analysis, and when a predetermined number (for example, 5 or more) is reached, only the measured value of the washing liquid is added and averaged. Specifically, the following is performed. That is, in the fifth lap, the measured value is corrected using the average value of the measured values of the cleaning liquid in the first, second, third, and fourth laps without using the cuvette blank value. In this case, the cuvette is at #N, the average value a _VNN when the wavelength is lambda _n is, a
_vNn = ( _a1Nn + _a2Nn + _a3Nn + _a4Nn ) / 4. The average value a _vNn thus obtained is used as correction data.

After the sixth lap, the previous
Average of the last four cleaning fluid measurements, including cleaning fluid measurements
Correct the measured value using the value. Note that here, "Latest
Of the four cleaning liquid measurements ”
The measured value of the cleaning solution after cleaning is the value after cleaning.
Since it cannot be used for correction,
Four cleaning liquid measurement values ”in the following order.
In this case, the measured values of the cleaning liquid in the m-th lap are, for example, as shown in Table 4.
As shown in FIG.₁ A corresponding to_m11, A _m21,
・ ・ 、 A_mN1 And the wavelength λ_Two ~ Λ_nCorresponding to
a_m12, A_m22, ..., a_mN2 Data string ~ a_m1n,
a_m2n, ..., a_mNnIs obtained. But
Therefore, on the (m + 1) lap, the cuvette is #N
And the wavelength is λ_nAverage value a in the case of_vNnIs a_vNn= (A
_{(m-3) Nn}+ A_{(m-2) Nn}+ A_{(m-1) Nn}+ A_mNn) / 4
You. Average value a thus obtained_vNnThe correction data and
To use.

[0033]

[Table 4]

According to the present embodiment, in a reaction vessel direct photometric automatic analyzer that cleans and reuses a reaction vessel that is also used as a measurement vessel, correction data for reducing the influence of contamination due to reuse is provided. Since a plurality of data (four data in the present embodiment) are averaged, the reliability of the correction data can be improved. As a result, the reliability of the measured value of the test sample is improved. The method of averaging a plurality of data according to the present embodiment focuses on the fact that when a new or clean reaction vessel is used, the reaction vessel is washed during the analysis, so that little contamination occurs with a small number of uses. It is.

In the first embodiment, the measured value of the cleaning liquid is used to obtain the correction data by averaging a plurality of data. However, instead of the measured value of the cleaning liquid, the difference between the previous measured value of the cleaning liquid and the cuvette blank value is used. A value may be used. The method of obtaining the actual correction data in that case is based on the method described above.

Next, a cuvette correction method according to a second embodiment of the present invention will be described. The configuration of the automatic analyzer used to execute the cuvette correction method of the second embodiment is the same as that of the first embodiment (see FIG. 1). Further, since the cuvette blank value is measured and stored before the start of the analysis, and the cleaning solution measurement value is measured and stored during the analysis, it is the same as in the first embodiment, so only the differences from the first embodiment will be described. Will be described.

First, the cuvette blank value and the measured value of the cleaning liquid are obtained and stored by the same method as in the first embodiment. Then, in the analysis of the first round, since a difference value described later cannot be obtained, correction is performed using, for example, only the cuvette blank value. On the other hand, in the second and subsequent rounds, a difference value between the previous measured value of the cleaning liquid and the above-mentioned cuvette blank value is obtained for each reaction vessel for each wavelength and stored sequentially. As a result, for example, for the wavelength λ _{1 in the} (m + 1) th round,
_{(A m11 -a 011), (} a m21 -a 02 1), ···,
A difference value sequence consisting of ( _{amN1- a0N1} ) is obtained.

Thereafter, a difference value relating to a predetermined number of cuvettes is selected from the difference value sequence (all numbers may be selected), and an average value of the difference values is obtained for each wavelength and sequentially updated and stored. As a result, for example, when the predetermined number (the number of data) is set to 10, the wavelength λ ₁ of the (m + 1) -th round
Is defined as S ₁ , S ₂ ,..., S ₁₀ immediately before calculation of the measured value of the test sample, a _v1 = (S ₁ + S ₂ +... + S ₁₀ ) / 10 As a result, an average value a _v1 of the difference values is obtained. The average value of the difference value thus determined as the correction data regarding the wavelength lambda _1, extracts the correction data corresponding to the wavelength used in the measurement of the test sample, to correct the measured value of the test sample.

The number of data used for averaging the difference values is, for example, 10 to 10 immediately before calculation of the measured value of the test sample.
The number is set to 30 or to a number corresponding to one round of the reaction table, or to an arbitrary number that is easily averaged as needed. Here, the data used for the averaging exceeds the number of reaction vessels for one round of the reaction table, and is overlapped so as to include the data for the last round (and, if necessary, for the past) from the latest round. May be collected. At this time, it is preferable to update the data so that the difference values for the number of data are used in the order from the latest data to the newest data.

According to the present embodiment, although the number of data of the measured values of the cleaning liquid is small immediately after the start of the analysis, a sufficient number of data are collected while waiting for the reaction time, so that the reliability of the correction value is improved with time. I do. The method of averaging a plurality of data in the present embodiment is particularly useful in a single-line, multi-test automatic analyzer in which a plurality of items are randomly analyzed in one reaction line. The focus is on the fact that the water is accumulated almost equally in all the containers without performing the operation.

Note that the present invention is not limited to the above-described example, and various changes or modifications can be made. For example, in a reaction vessel direct photometric automatic analyzer, comprising a plurality of reaction vessels, washing the plurality of reaction vessels and repeatedly using the same, and also using it as a measurement vessel to measure a test sample, the reaction vessel If the sample is new or clean, before the start of analysis, a step of measuring and storing a cuvette blank value for each of the wavelengths that may be used for the measurement for each reaction container in a state where the reaction container is filled with water. And, when washing the reaction vessel during the analysis operation,
A step of sequentially measuring and storing the cleaning liquid measurement values for each of the wavelengths that may be used for the measurement for each reaction container in a state in which the cleaning water is put in the reaction container, and the latest cleaning liquid measurement values including the previous cleaning liquid measurement values. The average value of two or more (for example, 2 to 10, practically 3 to 5, particularly 4 in this embodiment) measured values of the cleaning liquid is obtained for each of the wavelengths for each reaction vessel and sequentially updated and stored. And extracting memory data related to the wavelength of the reaction vessel used for measuring the test sample based on the average value, and correcting the measured value of the test sample from the stored data. Cuvette correction method (Appendix 1).

Further, in a reaction vessel direct photometric automatic analyzer, comprising a plurality of reaction vessels, washing the plurality of reaction vessels and using them repeatedly, and also using them as measurement vessels to measure a test sample, If the reaction vessel is new or clean, before the start of analysis, with water in the reaction vessel, measure the cuvette blank value for each of the wavelengths that may be used for measurement for each reaction vessel A step of storing and, when the reaction container is washed during the analysis operation, sequentially measuring a washing liquid measurement value for each of the wavelengths that may be used for measurement in a state where washing water is put in the reaction container for each reaction container. And the step of storing and calculating the difference between the previous cleaning liquid measurement value and the cuvette blank value for each of the wavelengths for each reaction vessel and storing the difference value,
At least 2 (5 or more practically, preferably 10
Or more, especially the total number of reaction vessels to be measured or 10 to 3
(0, 10 in the embodiment) the average value of the latest difference values for the reaction vessels for each of the wavelengths, sequentially updating and storing the average value, and the corresponding value used for measuring the test sample based on the average value. Cuvette correction method (additional item 2), comprising the steps of: extracting stored data relating to wavelength and correcting the measured value of the test sample from the stored data.

Further, in the above-described embodiment, the rotation stop angle of the reaction table is set to one round + cuvette, but within a range of p circumference ± 1 cuvette (p = 1, 2, 3,...). You may choose. In addition, other than that, a half circumference × r (r is an odd integer) ± 1 cuvette or U = wy ± z
(U is the number of cuvettes on the circumference of the reaction table, w is the number of cuvettes fed per one rotation stop angle, 1 / y is the approximate number of rotations at one rotation stop pitch, z is the sample or reagent dispensed when the cuvette is stopped. ), Or every N cuvettes (N is any integer from 1 to 4). In the second embodiment, the cuvette data counted from the cuvette to be analyzed in ascending order of the distance is used. However, the rotation stop angle of the reaction table is changed one by one intermittently. If the rotation is at a stop angle other than when proceeding (e.g., with an interval of every two or three cuvettes), the cuvette data counted in the order of the cuvettes that have been measured closer in time from the cuvette to be analyzed are used. It may be set to be used.

Further, in the above-described embodiment, the measurement using a plurality of all the wavelengths which may be used has been described. However, when the analysis items to be used in advance are determined within a predetermined time, It is also preferable to efficiently measure and correct by narrowing down to a required number of wavelengths selected based on input information from input means (keyboard, mouse, barcode reader, etc.). In addition, a reagent dispensing mechanism as dispensing means is provided with a washing water (eg,
If a mechanism is provided to wash the dispensing probe or nozzle by aspirating and / or discharging pure water, ion-exchanged water, etc., blank water for measuring a blank value is supplied to the reagent at the reagent dispensing position. The operation efficiency of the apparatus may be improved by configuring and controlling the dispensing mechanism to inject into the reaction vessel.

[0045]

As described above, according to the method of the present invention, in a reaction vessel direct photometric automatic analyzer that cleans and reuses a reaction vessel that is also used as a measurement vessel, contamination caused by reuse is eliminated. Since the data used for correction for reducing the influence is obtained by averaging a plurality of data, the reliability of the data can be improved. As a result, the reliability of the measured value of the test sample is improved.

[Brief description of the drawings]

FIG. 1 is a diagram illustrating a configuration of an automatic analyzer used for performing a cuvette correction method according to first and second embodiments of the present invention.

[Explanation of symbols]

 Reference Signs List 1 reaction table 2 reaction / measurement container (cuvette) 3 sampler 4 sample cup 5 first reagent table 6 reagent container 7 second reagent table 8 stirrer 9 photometric light beam

Claims (3)

[Claims] 1. An automatic analyzer of a reaction vessel direct photometry system comprising a plurality of reaction vessels, washing the plurality of reaction vessels and using the same repeatedly as a measurement vessel to measure a test sample, If the reaction container is new or clean, before the start of analysis, a step of measuring and storing a cuvette blank value for each reaction container with water in the reaction container, and the reaction container during the analysis operation A step of sequentially measuring and storing the measured values of the washing liquid for each reaction vessel in a state where the washing water is filled in the reaction vessel during the washing of the reaction vessel, and calculating an average value of a plurality of data including the cuvette blank value and the latest measured value of the washing liquid. Obtaining and sequentially updating and storing each of the wavelengths for each reaction vessel, and extracting storage data relating to the corresponding wavelength of the corresponding reaction vessel used for measurement of the test sample from the average value; Cuvette correction method characterized by comprising a step of correcting the measured value of the test sample based on the data. 2. An automatic analyzer of a reaction vessel direct photometry system comprising a plurality of reaction vessels, washing the plurality of reaction vessels and repeatedly using the same, and also using the same as a measurement vessel to measure a test sample. If the reaction vessel is new or clean, before the start of analysis, with water in the reaction vessel, measure the cuvette blank value for each of the wavelengths that may be used for measurement for each reaction vessel A step of storing, and at the time of cleaning the reaction vessel during an analysis operation, in a state where washing water is put in the reaction vessel, sequentially measure a cleaning solution measurement value for each of the wavelengths that may be used for measurement for each reaction vessel. And storing and calculating a difference value between the cleaning solution measurement value and the cuvette blank value for each of the wavelengths for each reaction vessel, and storing the average value of the difference values for the wavelength. Step of obtaining and sequentially updating and storing for each of the reaction vessels, extracting storage data related to the corresponding wavelength of the corresponding reaction vessel used for measurement of the test sample from the average value, and extracting the test sample based on the stored data. Correcting the measured value of cuvette. 3. A reaction vessel direct photometric automatic analyzer comprising a plurality of reaction vessels, washing the plurality of reaction vessels and repeatedly using the same, and also using the same as a measurement vessel to measure a test sample. If the reaction vessel is new or clean, before the start of analysis, with water in the reaction vessel, measure the cuvette blank value for each of the wavelengths that may be used for measurement for each reaction vessel A step of storing, and at the time of cleaning the reaction vessel during an analysis operation, in a state where washing water is put in the reaction vessel, sequentially measure a cleaning solution measurement value for each of the wavelengths that may be used for measurement for each reaction vessel. And storing and calculating the difference value between the previous cleaning liquid measurement value and the cuvette blank value for each reaction vessel for each of the wavelengths, and corresponding to the latest storage data Obtaining the average value of the difference values for a plurality of reaction vessels including the reaction vessel to be performed for each of the wavelengths and sequentially updating and storing the average value, and extracting storage data relating to the wavelength used for measurement of the test sample from the average value. Correcting the measured value of the test sample based on the stored data.