JPH0348165B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention relates to cosmetics containing fibronectin, which is a type of glycoprotein extracted from living organisms. More specifically, the present invention relates to a cosmetic containing fibronectin, which has skin revitalizing, moisturizing, and whitening effects on the skin, and hair nourishing and nourishing effects on the hair. Conventionally, hormones have generally been used as skin metabolism promoters and hair nourishing agents. That is, follicle hormones, adrenal skin hormones, and the like have been added to rejuvenate the skin, prevent wrinkles, and promote hair growth. However, when hormones are used in large amounts, there are problems such as systemic side effects and thickening of the epidermis. In view of the above-mentioned circumstances, the present inventors have conducted intensive research to search for safe and highly medicinal substances and to incorporate them into cosmetics. As a result, we have discovered that fibronectin, a biological extract, has excellent properties without the above-mentioned drawbacks. The inventors have discovered that the present invention has the following effects, and have completed the present invention. That is, the present invention provides a cosmetic composition characterized by containing fibronectin. Hereinafter, the configuration of the present invention will be explained in detail. Fibronectin used in the present invention
Fibroblast membrane protein (fibroblast membrane protein) is a type of glycoprotein extracted from living organisms, and is also called cold insoluble globulin (CIG).
Up until now, it has been referred to as large external trypsin-sensitive protein, cell surface protein, adhesive stoma, or obsonic alpha ₂ surface-binding glycoprotein, but recently it has been generally referred to as fibronectin or cold-insoluble globulin. Fibronectin exists not only in the blood but also in mesenchymal cells such as fibroblasts and basement membranes such as the epidermis. The molecular weight of what is present in blood
It has a dimer structure consisting of two 200,000 to 220,000 monomers, and is contained in blood at a concentration of about 0.3 mg/ml. Those present in mesenchymal cells and basement membranes range from 200,000 to 25
It has a structure in which monomers with a molecular weight of 10,000 yen form a dimer or a multimer of more than 1,000 yen. The former is commonly called blood fibronectin, and the latter is commonly called cellular fibronectin. Other known physicochemical properties of fibronectin include its mobility as _α2 globulin and its isoelectric point as
5.0, molecular extinction coefficient A1% 1cm280nm is 12.9-13.0,
Examples include S ⁰ ₂₀ , W of 11 to 14S, and sugar content of 5%. Note that fibronectin is composed of several domains, each of which has a specific structure that interacts with, for example, transglutaminase, heparin, actin, fibrin, cells, and collagen. As mentioned above, fibronectin is present in human or animal cells or bloodstream. Extraction may be carried out according to conventional methods. For example, if cultured cells are treated with 0.2M urea, a 5-10% fibronectin solution can be obtained. In addition, fibronectin in blood can be extracted by processing with gelatin-denatured affinity chromatography. The appropriate amount of fibronectin in the present invention is generally 0.00001 to 0.1% by weight based on the total amount of the cosmetic. In addition to the above-mentioned essential ingredients, the cosmetic of the present invention may contain oil, surfactants, pigments, fragrances, preservatives, moisturizing agents,
Ultraviolet absorbers, pigments, antioxidants, and other chemicals can be added. The cosmetic obtained according to the present invention exhibits excellent hair growth and growth effects when applied to the scalp, and provides moisturizing properties and exhibits whitening and skin revitalizing effects when applied to the skin. Next, the present invention will be explained in more detail with reference to Examples. Note that the present invention is not limited to this. The blending amount is in weight%. Example 1 Lotion (wt%) Fibronectin 0.01 Glycerin 3.0 1-3-butylene glycol 4.0 Ethanol 8.0 Polyoxyethylene oleyl alcohol 0.5 Methylparaben 0.1 Citric acid 0.01 Sodium citrate 0.1 Fragrance 0.05 Purified water 84.23 Production method: Add citric acid to purified water , soda citrate,
Dissolve glycerin, fibronectin, and 1-3 butylene glycol. Separately, polyoxyethylene oleyl alcohol, fragrance, and methylparaben were dissolved in ethanol, and this was added to the above-mentioned purified aqueous solution to solubilize and filter to obtain a lotion. [Whitening effect] In order to examine the whitening effect of fibronectin, the melanin production inhibiting effect of the lotion obtained in Example 1 was investigated. First, the lotion produced in Example 1 was used as a sample, and a blank containing no fibronectin (substituted with purified water) was prepared. Add agent, 10
Place in a constant temperature bath for a minute. Next, add the tyrosinase aqueous solution, stir, and set the spectrophotometer.
Absorbance at 475 mμ was measured over time. 475
An increase in mμ absorbance indicates that L-tyrosine is oxidized and doperchrome, a precursor of melanin, is produced. In other words, a small amount of Doperchrome produced means that it has the effect of suppressing melanin production. The measurement results are shown in Figure 1. This result shows that fibronectin has a melanin suppressing effect. [Wound healing effect] Six-week-old Wistar rats were divided into groups of five, shaved, and then subjected to the test. After the rat was anesthetized, a skin incision of approximately 2 cm was made on the back, and fibronectin 0.1% saline solution was immediately applied to the incision. After 3 weeks, the rats were killed by buffeting, and the suture needle was removed, and a skin section measuring 1 cm in diameter was prepared. Using this sample, the cutting tension was measured using Tensilon UTM-4 (manufactured by Toyo Saiki Co., Ltd.). The higher the tension, the better the cut surface is healed. As a control, physiological saline was applied.
The results are shown in Table 1.

[Table] The results show that fibronectin has a wound healing effect and a skin revitalizing effect. [Usability and Safety Test] Using the lotion produced in Example 1, and using the one without fibronectin as a blank, a sensory test of the feeling of use was conducted for each lotion. The results are shown in Table 2.

[Hair growth test]

One group consisted of five C57BL mice. The mice were shaved from both sides of their abdomens to their backs, and a saline solution containing 0.1% fibronectin was applied to one side, and a saline solution containing no fibronectin was applied to the other side. 2
Measure the length of hair that has grown in the shaved area after a week.
It is shown in Table 3. The higher the number, the better the hair growth effect.

[Table] The results of this experiment clearly showed that fibronectin has a hair-nourishing effect. Example 2 Cream (% by weight) Fibronectin 0.01 1-3 butylene glycol 5 Cetyl alcohol 4 Reduced lanolin 5 Squalane 35 Stearic acid glyceride 2 Polyoxyethylene sorbitan monolaurate 2 Methylparaben 0.1 Ethylparaben 0.15 Beeswax 5 Fragrance Appropriate amount of purified water Remainder Example 3 Hair tonic (wt%) Fibronectin 0.0001 Aspergillus japonica extract 0.5 Salicylic acid 0.3 Ethinylestradiol 0.0005 Ethanol 40 Fragrance/Pigment Appropriate amount of purified water Remaining Example 4 Hair tonic (wt%) Fibronectin 0.001 Glycyrrhetinic acid 0.1 Resorcinol 0.8 Nicotinic acid Benzyl 0.5 l-menthol 0.1 Ethanol 70 Perfume/Pigment Appropriate amount Purified water Remaining Examples 2, 3, and 4 all had excellent hair-nourishing or moisturizing effects, and had no problems in terms of skin safety.

[Brief explanation of drawings]

Figure 1 shows the effect of suppressing melanin production (L
- Measured by the amount of doperchrome, a precursor of melanin, surviving from tyrosine).

Claims (1)

[Claims] 1 Cosmetics characterized by containing fibronectin