JPH0313871B2 - - Google Patents
Info
- Publication number
- JPH0313871B2 JPH0313871B2 JP61050897A JP5089786A JPH0313871B2 JP H0313871 B2 JPH0313871 B2 JP H0313871B2 JP 61050897 A JP61050897 A JP 61050897A JP 5089786 A JP5089786 A JP 5089786A JP H0313871 B2 JPH0313871 B2 JP H0313871B2
- Authority
- JP
- Japan
- Prior art keywords
- cellulase
- cellulases
- immobilized
- solution
- hours
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010059892 Cellulase Proteins 0.000 claims description 33
- 229940106157 cellulase Drugs 0.000 claims description 29
- 108010084185 Cellulases Proteins 0.000 claims description 24
- 102000005575 Cellulases Human genes 0.000 claims description 24
- 230000000694 effects Effects 0.000 claims description 19
- 108010002430 hemicellulase Proteins 0.000 claims description 17
- 239000000126 substance Substances 0.000 claims description 17
- 229920002678 cellulose Polymers 0.000 claims description 13
- 239000001913 cellulose Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 7
- 229940059442 hemicellulase Drugs 0.000 claims description 4
- 230000000593 degrading effect Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 description 23
- 239000000243 solution Substances 0.000 description 22
- 239000002023 wood Substances 0.000 description 14
- 239000008351 acetate buffer Substances 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 10
- 238000000034 method Methods 0.000 description 10
- 239000000463 material Substances 0.000 description 8
- 235000013312 flour Nutrition 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 235000000346 sugar Nutrition 0.000 description 6
- 240000000731 Fagus sylvatica Species 0.000 description 5
- 235000010099 Fagus sylvatica Nutrition 0.000 description 5
- 108010047754 beta-Glucosidase Proteins 0.000 description 5
- 102000006995 beta-Glucosidase Human genes 0.000 description 5
- 238000000354 decomposition reaction Methods 0.000 description 5
- 240000005020 Acaciella glauca Species 0.000 description 4
- 235000018782 Dacrydium cupressinum Nutrition 0.000 description 4
- 235000013697 Pinus resinosa Nutrition 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 241000223261 Trichoderma viride Species 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000609240 Ambelania acida Species 0.000 description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 240000000111 Saccharum officinarum Species 0.000 description 2
- 235000007201 Saccharum officinarum Nutrition 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000010905 bagasse Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000000123 paper Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- HMUNWXXNJPVALC-UHFFFAOYSA-N 1-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C(CN1CC2=C(CC1)NN=N2)=O HMUNWXXNJPVALC-UHFFFAOYSA-N 0.000 description 1
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 1
- BTJIUGUIPKRLHP-UHFFFAOYSA-M 4-nitrophenolate Chemical compound [O-]C1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-M 0.000 description 1
- IFBHRQDFSNCLOZ-RMPHRYRLSA-N 4-nitrophenyl beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C([N+]([O-])=O)C=C1 IFBHRQDFSNCLOZ-RMPHRYRLSA-N 0.000 description 1
- CONKBQPVFMXDOV-QHCPKHFHSA-N 6-[(5S)-5-[[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]methyl]-2-oxo-1,3-oxazolidin-3-yl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C[C@H]1CN(C(O1)=O)C1=CC2=C(NC(O2)=O)C=C1 CONKBQPVFMXDOV-QHCPKHFHSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 101710178139 Endoglucanase 8 Proteins 0.000 description 1
- 101710112457 Exoglucanase Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5089786A JPS62208282A (ja) | 1986-03-07 | 1986-03-07 | 固定化セルラ−ゼ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5089786A JPS62208282A (ja) | 1986-03-07 | 1986-03-07 | 固定化セルラ−ゼ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62208282A JPS62208282A (ja) | 1987-09-12 |
| JPH0313871B2 true JPH0313871B2 (no) | 1991-02-25 |
Family
ID=12871530
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5089786A Granted JPS62208282A (ja) | 1986-03-07 | 1986-03-07 | 固定化セルラ−ゼ |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62208282A (no) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2505657A1 (en) | 2009-11-27 | 2012-10-03 | Mitsui Chemicals, Inc. | Process for production of monosaccharide |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59173079A (ja) * | 1983-03-18 | 1984-09-29 | Agency Of Ind Science & Technol | セルラ−ゼ類の回収方法 |
-
1986
- 1986-03-07 JP JP5089786A patent/JPS62208282A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59173079A (ja) * | 1983-03-18 | 1984-09-29 | Agency Of Ind Science & Technol | セルラ−ゼ類の回収方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62208282A (ja) | 1987-09-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hägerdal et al. | Saccharification of cellolulose by the cellulolytic enzyme system of Thermonospora sp. I. Stability of cellulolytic activities with respect to time, temperature, and pH | |
| JP5681923B2 (ja) | 高濃度糖化液の製造方法 | |
| JPS5839517B2 (ja) | セルロ−スカラ アルコ−ルオセイゾウスル ホウホウ | |
| GB2047709A (en) | Purification of cellulase | |
| AU2003208215A8 (en) | Method for glucose production using endoglucanase core protein for improved recovery and reuse of enzyme | |
| Sim et al. | Spent brewery grains as substrate for the production of cellulases by Trichoderma reesei QM9414 | |
| Biely et al. | Xylan-degrading activity in yeasts: growth on xylose, xylan and hemicelluloses | |
| JPH01256394A (ja) | セロオリゴ糖の酵素的製造方法 | |
| US4713334A (en) | Process for the saccharification of celluloses | |
| Deschamps et al. | β-Glucosidase production by Aspergillus phoenicis in solid state fermentation | |
| Elgharbawy et al. | Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system | |
| Lepcha et al. | Glycoside hydrolases from a thermophilic microbial consortium and their implication in the saccharification of agroresidues | |
| US4378432A (en) | Process for manufacturing sweetened liquors and derivatives thereof from cellulose-containing vegetable substrates | |
| JPH0313871B2 (no) | ||
| CA1277621C (en) | Process for producing immobilized beta-glucosidase | |
| Singh et al. | Saccharification of cellulosic substrates by Aspergillus niger cellulase | |
| JP4257403B2 (ja) | 酵素処理方法 | |
| JPS60244294A (ja) | セルロ−スから高濃度アルコ−ルを半連続的に製造する方法 | |
| JPS59213396A (ja) | セルロースの糖化方法 | |
| Moosavi-Nasab et al. | Cellulase production by Trichoderma reesei using sugar beet pulp | |
| JP2014090707A (ja) | リグノセルロース含有バイオマスの酵素糖化処理方法及びリグノセルロース含有バイオマスからのエタノール製造方法 | |
| JPH0683674B2 (ja) | セロビオ−スの製造法 | |
| JPH026514B2 (no) | ||
| Puntambekar | Cellulase production by the edible mushroom Volvariella diplasia | |
| Sakamoto et al. | Reduced cellulose as a substrate of cellulases |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |