JPH03123733A - Skin external preparation - Google Patents

Abstract

PURPOSE:To obtain a skin external preparation capable of preventing injury of skin cells by ultraviolet ray and having a preventive effect on aging of skin by blending water-extracted material of mammalian spleen with amino acid (derivative). CONSTITUTION:A mammalian spleen e.g. fresh frozen spleen of cattle is pulverized and extracted with >=60 deg.C hot water for >=1hr and further heated at >=80 deg.C for >=10min and filtered and concentrated. Ethyl alcohol is added to the concentrate to remove protein and further the mixture is filtered to clarify the mixture. Then the clarified solution is subjected to an ultrafiltration using a capillary membrane filtration system having 2000 separation limit molecular weight to provide water-extracted material of spleen. 0.0001-5.0wt.% (as dried solid content) of the water-extracted material and 0.005-5.0wt.% of one or more substance selected from amino acid and amino acid derivative are mixed into total amount of skin external preparation.

Description

Detailed Description of the Invention [Industrial Application Field] The present invention is characterized by blending an aqueous extract of mammalian spleen with one or more selected from amino acids and amino acid derivatives. This invention relates to an external skin preparation that has the effect of protecting skin cells (fibroblasts, epidermal cells) from damage and preventing skin aging.

[Conventional technology] Conventionally, in order to prevent skin aging, various moisturizers have been added to maintain the moisture balance on the skin surface, and various vitamins and vitamins have been added to improve the function of cells within the skin. There were products that contained plant extracts, etc., but their effects were not yet sufficient.

[Problems to be Solved by the Invention] As a result of intensive research into whether there is a method for efficiently preventing skin aging, the present inventors discovered that the present inventors have developed a method for effectively preventing skin aging, and found that the present inventors have developed a method for effectively preventing skin aging. By combining one or more types, it prevents damage to skin cells (fibroblasts, epidermal cells) caused by ultraviolet rays,
The present inventors have discovered that skin aging can be significantly prevented, and have completed the present invention.

[Means for Solving the Problems] That is, the present invention is an external skin preparation characterized by blending an aqueous extract of mammalian spleen with one or more selected from amino acids and amino acid derivatives.

Hereinafter, the configuration of the present invention will be explained in detail.

The aqueous extract of mammalian spleen used in the present invention can be obtained, for example, by the following method.

It is an extract extracted with water from the liver of salivary mammals such as cows and pigs, and is known as Revitalin or Revitalin-P (manufactured by Pentafarm), and this is the most preferred.

Extraction may be carried out according to conventional methods; for example, as follows.

New 1! The frozen spleen of a cow is crushed, extracted with hot water at 60°C or higher for over 1 hour, roughly heated at 80°C or higher for 10 minutes or more, then filtered and concentrated. a) Add edyl alcohol to the condensate to remove protein and clarify by rough filtration. Next, ultrafiltration is performed using a capillary membrane filtration system with a separation limit molecular weight of 2000.

The blending amount of the extract used in the present invention is 0.0001 to 5.0% by weight as dry solid content in the total amount of the skin external preparation.

In the present invention, in addition to the above extract, one or more amino acids and amino acid derivatives are blended.

The amino acids and amino acid derivatives used in the present invention are:
Glycine, alanine, valine, leucine, isoleucine, serine, threonine, aspartic acid and its salts,
Glutamic acid and its salts, lysine, arginine, cysteine, cystine, medione, phenylalanine, tyrosine, histidine, tryptophan, proline, N-
N-acyl acidic amino acid salts such as valmitoyl mono-diethyl aspartate, N-coconut fat rib F2-L-sodium glutamate, acyl neutral amino acid salts such as coconut oil fatty acid sarcosine triethanolamine, sodium lauroylmethyl-β-alanine, These include pyrrolidone carboxylic acid and its salts, POE (40) hydrogenated castor oil monopyroglutamic acid monoisostearic acid diester, coconut oil fatty acid-L-arginine ethyl ester-DL-pyrrolidone carboxylate, and the like.

The blending amount of amino acids and amino acid derivatives in the present invention is 0.005 to 5.0% by weight based on the total amount of the skin external preparation.

In addition to the above-mentioned essential ingredients, the external skin preparation of the present invention contains other ingredients normally used in external skin preparations such as cosmetics and pharmaceuticals, such as vitamin A oil, retinol, vitamin A such as retinol acetate, riboflavin, and riboflavin butyrate. , vitamin 82 such as flavin adenine dinucleotide, 86 vitamins such as pyridoxine hydrochloride, pyridoxine dioctanoate, L-ascorbic acid, L-ascorbic acid civalmitic acid ester, L-ascorbic acid-2-sulfuric acid Vitamin E such as Ha, calcium pantothenate, D
- Pantothenic acids such as bantothenyl alcohol, bantothenyl ethyl ether, acetyl bantothenyl ethyl ether, vitamin Ds such as ergocalciferol and cholecalciferol, nicotinic acid, nicotinic acid amide,
Nicotinic acids such as benzyl nicotinate, vitamin E such as α-tocopherol, tocopherol acetate, DL-α-tocopherol nicotinate, DL-α-tocopherol succinate, vitamins such as vitamin P, biotin, avocado oil, palm oil , oils such as peanut oil, beef tallow, rice bran oil, jojoba oil, evening primrose oil, carnauba wax, lanolin, liquid paraffin, squalane, isostearyl palmitate, isostearyl alcohol, glycerin tri-2-edylhexanoate, glycerin, sorbitol,
Moisturizing agents such as polyethylene glycol, 1,3-butylene glycol, collagen, hyaluronic acid, chondroitin sulfate, sodium dextran sulfate, amyl valadimethylaminobenzoate, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid Ha, urocanic acid , UV absorbers such as diisoprobyl ethyl cinnamate, antioxidants such as sodium erythorbate, parahydroxyanisole, sodium stearyl sulfate, jetanolamine cetyl sulfate, cetyl trimethylammonium saccharin, polyethylene glycol isostearate, glyceryl arachinate, diglycerine diisostearate,
Surfactants such as phospholipids, preservatives such as ethylparaben and butylparaben, glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, salicylic acid derivatives, anti-inflammatory agents such as hinokitiol, zinc oxide, allantoin, placenta extract, glutathione, saxifrage extract, etc. skin whitening agents, sagebrush, orensis, citrus, peonies, sensuals, birch, sage, loquat, carrots, aloe, mallow, iris,
Activators such as extracts of grapes, lily pads, loofahs, lilies, saffron, nebula, perforatum, hypericum, ononis, rosemary, garlic, etc., royal jelly, photosensitizers, cholesterol derivatives, larval blood extracts, etc., 7
- Blood circulation promoters such as ozanol, sulfur, antiseborrheic agents such as dianthol, thickeners such as carboxyvinyl polymer, riboxymethyl cellulose, carboxyhydroxypropyl cellulose, fragrance, water, alcohol, titanium yellow cursamine, Colorants such as safflower red, polyethylene,
Resin powder such as nylon or the like can be appropriately blended as needed.

Further, the dosage form of the skin external preparation of the present invention is arbitrary, and may be, for example, a solubilized system such as a lotion, an emulsified system such as a milky lotion or a cream, or a dosage form such as an ointment or a dispersion.

[Effect of the invention] Next, the effects of the invention will be shown using experimental data.

The number of skin 1a cells damaged by ultraviolet rays was determined by the method shown below, and the protection rate was calculated from that number.

(Method) eA fibroblasts, one of the skin cells, were cultured in a single sugar syrup using the Growth method using a Dulbecco modified Eagle culture. ! (DMEM medium) for passage maintenance.

Next, these cells were treated with trypsin-EDTA to prepare a cell suspension, and a certain number of culture dishes (Falco
The cells were sown on 300 to 135 mm squares and cultured in a CO2 incubator (37°C, 5% CO2). After culturing for a certain period of time, discard the medium in the Petri dish and add phosphate buffer (2 ml/Petri dish)
Wash the cells twice with Next, each of the following samples was added.

Control Phosphate buffer solution (2 ml/Petri dish) Sample ■ Phosphate buffer solution containing Revitalin P (Pentapharm) adjusted to 1.0% by weight (2 ml/Petri dish) Sample ■ L-aspartic acid 0.5% by weight Phosphate buffer solution adjusted to 0.5% by weight of L-arginine (2 ml/Petri dish) Sample ■ Buckwheat weight phosphorus 2015 weight% L-aspartic acid 0.25% by weight Phosphorus adjusted to 0.25% by weight of L-arginine Acid buffer solution (2 ml/Petri dish) Two copies of each of the above samples were prepared, and one was irradiated with ultraviolet rays.

For ultraviolet rays, use a Toshiba BLB lamp (TO3I (IBA FL)
20S-BLI3UV-A region peak 3 (55nm) and Toshiba SE lamp (TO3III BATOREX FL2
Irradiation was performed at a position 16 cm from the Petri dish using OSE-30 UV-B region peak 305 nm). The irradiation amount is determined in advance by UV-RADIOMETER (Eisai & Tore
The intensity of ultraviolet rays was measured in advance using a UV light source (manufactured by X), and calculations were made from the average intensity and irradiation time. In addition, the ultraviolet rays actually irradiated to the cells were converted by taking into account the absorption by the Plus Deck petri dish and phosphate buffer. (UV-A reduction rate 52χ, UV-
(Reduction rate of B: 21%) Next, discard the phosphate buffer for both non-irradiated and irradiated samples, and
Two liters of MEM medium were added and cultured for a certain period of time.

After culturing, the amount of DNA in the cells was measured by the method of Ce5ar & Kenneth, the number of cells was converted using a standard curve, the survival rate of the cells was determined from the following formula, and this was evaluated as the protection rate.

The above results indicate that the combination of daffodil extract from the spleen of clear mammals and one or more selected from amino acids and amino acid derivatives significantly protects skin cells from damage caused by ultraviolet rays.

[Example] Next, the present invention will be explained in detail with reference to an example. still,
The present invention is not limited thereby. The formulation dish is in weight percent.

Example 1 Lotion (1) Revicrin-P (manufactured by Pentafirm) (2) L-arginine (3) Glycine (4) Tocopherol acetate (5) Glycerin (6) 1,3-butylene glycol (7) Ethanol (8) ) Polyoxyethylene oleyl alcohol (9) Methylparaben (10) Citric acid (11) Sodium citrate (12) Fragrance 0.05 0.05 0.03 0.01 4.0 4.0 7.0 0.5 0 .2 0.05 0.1 0.05 (13) Purified water remainder (manufacturing method) Citric acid, sodium citrate, glycerin, 1,
Dissolve 3-butylene glycol, Revitalin-P, L-arginine, and glycine. Separately, polyoxyethylene oleyl alcohol, tocopherol acetate, fragrance, and methylparaben were dissolved in ethanol, and this was added to the above-mentioned purified aqueous solution to solubilize and filtered to obtain a lotion.

Example (1) (2) (3) (4) (5) (6) 2 Cream Cetostearyl Alcohol Squalane Beeswax Reduced Lanolin Ethylparaben Polyoxyethylene (20) Sorbitan Monopalmitate 3.5 40.0 3. 0 5.0 0.3 2.0 (7) Stearic acid monoglyceride 2.0(8)N
- Stearoylglutamate Ha 0.5 (9) Revitalin 0.5 (Pentapharm) (10) Retinol acetate 2.0 (11
) Evening primrose oil 0.05 (12) Fragrance 0.03 (13) L-
Aspartic acid 0.25(14) L-ce')'/0.75(F)1
*3-Butylene glycol 5.0 (16) Polyethylene glycol 1500 5.0 (17) Purified water
Residue (manufacturing method) (1) (2) (3) (4) (5) (6) (
7) Add and dissolve (8) and (10) (11) (12) and heat to 75℃ (9) (13) (14)
(15) Add to (13) and (17) while stirring. After processing with a homomixer to make emulsified particles fine, the mixture was rapidly cooled while stirring to obtain cream.

Example 3 Emulsion (1) Revitalin-P (2) L-ascorbic acid-2-sulfuric acid Ha (3) Stearic acid (4) Cetyl alcohol (5) Beeswax (6) Polyoxyethylene (10) Monooleic acid ester (7) ) Quince seed extract (5% aqueous 1) (8) L-arginine (9) L-glutamic acid Ha (10) P CA -Ha (11) Hyaluronate Na (12) Placenta extract (13) Propylene glycol (14) ) Ethanol (15) Ethylparaben (16) Fragrance f:I (17) Purified water 0.01 0.005 1.5 0.5 2.0 1.0 20.0 0.01 0.02 0.05 0 .1 0.05 5.0 3.0 0.3 0.03 Residue (manufacturing method) Add aroma fumi I to ethanol and dissolve (alcohol phase). Purified water with propylene glycol and hyaluronic acid Ha
1 Add Revitalin-P, L-arginine, Shi-glutamic acid Ha, PCA-Ha, and placenta extract, dissolve by heating, and keep at 70°C (aqueous phase). Mix other ingredients except quince seed extract, heat and dissolve and keep at 70℃ (
oil phase). Pre-emulsify by adding the oil phase to the water phase, and homogeneously emulsify using a homomixer. Add the alcohol phase and quince seed extract while stirring. Thereafter, the mixture was cooled to 30° C. while stirring to obtain a milky lotion.

(7) Propylene glycol 7.0 (8 ethanol 10.0 (9 methyl paraben)
0.05 (10 fragrance
0.05 (11 Purified water Remaining process) Polyethylene glycol, propylene glycol, methylparaben, Revitalin-P1L-arginine 1.L-valine and cholecalciferol are added to the purified water and dissolved with stirring.Next, polyvinyl alcohol is added and stirred with heating. Ethanol in which the fragrance was dissolved was added and dissolved with stirring to obtain a pack.

Example (1) (2) (3) (4) (5) (6) 4 Pack Revitalin-P Cholecalciferol Polyvinyl Alcohol L-Arginine L-valine Polyethylene Glycol 1.0 0.01 15.0 1.0 1.0 3.0 Example (1) (2) (3) (4) (5) (6) 5 Ointment Revitalin-PL L-arginine L-lysine Tocopherol acetate Retinol palmitate Stearyl alcohol 0.5 0.8 2 .0 1.0 0.5 18.0 (7) Mokuro 20.0 (8) Polyoxyethylene (10) Monooleate 0.25 (9) Glycerin monostearate 0.3 (10) Vaseline 40 .0(11
) Purified water remainder (manufacturing method) Keep purified water at 70°C (water phase) and mix and dissolve the following components at 70°C (oil phase). The oil phase was added to the water phase, uniformly emulsified using a homomixer, and then cooled to obtain an ointment.

Claims (1)

[Claims] 1. A skin preparation for external use, comprising a mammalian spleen aqueous extract and one or more selected from amino acids and amino acid derivatives.