JPH0242012A - Preparation for hair - Google Patents

Abstract

PURPOSE:To obtain a preparation for hair inhibited in occurrence of allergic dermatitis, enabling long-term continuous use and capable of expecting effect for preventing dander and pruritus and hair growth promoting effect by blending henna leaf powder, henna leaf powder extract or lauson at a specific amount. CONSTITUTION:Henna leaf powder, henna leaf powder extract or lauson is incorporated into a preparation for hair such as hair shampoo, hair rinse, permanentwave treating agent, hair cream, hair tonic, hair liquid or hair spray, etc., in low concentration of 0.017-0.035wt.% expressed in terms of the content of lauson to provide the preparation for hair capable of improving safeness by cutting allergy induction ability to skin and preventing dander and pruritus, enabling long-term continuous use and capable of expecting hair grow promoting (hair growing or hair nouring) effect.

Description

DETAILED DESCRIPTION OF THE INVENTION [1] Object of the Invention The present invention relates to a hair preparation using henna leaf powder, its extract, or lausone.

In the hair preparation according to the present invention, the content of lauson contained in the formulation can be specified to be 0.017% to 0.035%, and by using it within this specified range, the occurrence of allergic skin inflammation can be suppressed. be done.

Industrial Application Fields As described above, the present invention can be used in preparations for the scalp or hair.

As specific products (preparations), it can be used in the following well-known cosmetics and quasi-drugs. Furthermore, since safety is ensured, it can also be incorporated into skin preparations.

(Combined preparations) Hair shampoo, hair conditioner, permanent waving agent, hair cream, hair tonic, hair liquid, hair spray, etc., pomade, hair dye, hair growth promoter, etc.

1. Conventional technology Henna refers to plants of the genus Chiffonaceae of the Lythraceae family, such as Lawsonia alba, Lawsonia einelmis, and Lawnia spinosa. For example, in India, one of its origins. Since ancient times, liver, viscera,
It is said to have been used to treat urinary problems, bladder stones, pellagra, and stubborn skin diseases.

On the other hand, in Europe, it has a long history of being used as a hair dye to dye hair blonde.

In addition, as a dyeing substance for this purpose, a naphthoquinone pigment contained in henna leaves: Lauson (2-hydroxy-1,4-naphthoquinone bimolecules! 174.5, red needle-like crystals, decomposed at 190°C) is used. , is known as one of its components.

Regarding lauson, some literature states that henna leaves contain about 1%, but according to analysis by the present inventors, the total amount of various naphthoquinone components is about 1%.
% (0.7±0.3%), and it seems that there are very few cases that contain 1% as a round.

Lauson has also been obtained by synthetic methods, and its use is considered as a dye or an intermediate for pharmaceuticals.

Publications known in Japan that describe the use of henna stool as a hair dye include, for example, the following documents:

(Location of publications) (1) Tetsusaku Ikeyo: Cosmetic Science 9.259 1968 (
Nanzando) (2) Chotabe Tsukamoto: Cosmetic Science p. 99 (1971)
(Nankodo) (3) Tatsuo Karimai: Folk Medicine of the Sukekai, p. 78 (Showa Bookstore) (4) Nobuo Tsuchiya: Developing hair dye (Hair Gui) using henna leaves in 1978 February 10th (-Maru Boeki) The present inventors have already published the above-mentioned publication (4) with the aim of using henna leaves as a hair dye, and have investigated the specific application of the hair dyeing effect. There is a history. Zunawachi,
There have been reports that hair dyes such as para-phenylenediamine often induce allergic dermatitis in a limited number of people. We have been conducting research on hair dyeing effects using henna leaves, henna leaf extracts (extracts), or lauson purified from henna leaf extracts.

For example, in Japan, the above-mentioned publications (1) to (3) indicate the use of henna leaves as a hair dye, but there is little data specifically shown regarding its hair dyeing effect. Therefore, in the above-mentioned publication (4), the present inventors took into consideration the fact that many people of Asian and African descent, including the Japanese, have black hair, and considered the condition of dyeing when using it specifically. I became interested in whether it would dye hair, so I conducted a specific hair dyeing experiment to see if it could be used as a hair dye in Japan.

As a result, black hair can be dyed a dark brown color, and one method for dyeing black hair requires an auxiliary agent, such as 1% FeC2, which is best used in combination with an aqueous solution. Also, in order to dye the hair blonde, it is necessary to bleach the black hair in advance, and of course, even with these bleaching treatments, the hair turns brown.
After this, if you dye with henna leaves, you will get beautiful chestnut hair with a reddish tinge.
It has been found that if hair is dyed using eC15 aqueous solution in combination, hair can be dyed dark chestnut. In other words, henna leaf powder, henna leaf extract, or lauson can be used by people with black hair in the same way as hair dyes containing paradiphenylene amine, and for hair that has been dyed. It was confirmed that gloss and flexibility were imparted.

In addition, in the above-mentioned Publication (4), the present inventors have discovered that henna leaves, henna leaf extracts ( #IP has determined that there are no safety issues for the skin or scalp, and that it can be used as a material that can be substituted for para-diphenylene diamine. Furthermore, in addition to hair dyeing effects, henna leaves, henna leaf extracts, and laucon have secondary effects or effects such as repairing damaged hair and reducing dandruff and sagging on the scalp. It was also stated that the use of henna leaves is promising in Japan as well.

Since then, the application of henna leaves as a hair dye^- has attracted attention from Japanese cosmetic engineers, and Japanese pharmaceutical and cosmetic raw material trading companies have received successive sales pitches for henna leaves from overseas. There is a history.

However, since then, research has been conducted on henna leaves, henna leaf extracts, and lauson, and hair dyes and hair preparations that contain (use) them, but Japanese manufacturers have not yet responded. Not commercially available.

However, when we further investigated the current situation in Japan, we found that with the rapid increase in the number of overseas travelers, some items brought back from overseas include 1 ton of henna leaf extract and more.
There are hair rinses, hair shampoos, etc. that contain henna, and I have heard that some people personally obtain henna leaves and use them as hair dyes through word of mouth.

This is closely related to the recent trend toward natural or retro trends, and shows that consumers are highly interested in herbs.

Problems to be Solved by the Invention The present inventors conducted a series of safety evaluations based on the above-mentioned circumstances when applying henna leaves to hair dyes. In other words, given the history in Europe and India, it is assumed that immunological allergic reactions such as those seen with para-phenylenediamine are rare.

If there is, there is a possibility that it will induce rashes and itching, but on the other hand, it will not have the effect of suppressing rashes and itchiness.
It is believed that henna leaves are difficult to obtain, and in fact, henna leaves are now being added to conditioners and shampoos for their hair dyeing effects as well as for preventing itching and dandruff.

However, in providing the raw material, the present inventors made it clear that one of the main components contained in henna leaves is a naphthoquinone pigment, which has been used in India. Judging from the efficacy or effect, the naphthoquinone pigment component is thought to be deeply involved in the pharmacological action. In addition to conducting a re-evaluation test for skin safety using
Conducted experiments such as mutagenicity tests.

As a result, it was confirmed that there was no mutagenicity at all. However, contrary to the inventors' expectations, in a sensitization experiment using an immunological method (maximization method), an extremely mild erythema was observed in the henna leaves, although it is difficult to conclude that the result was positive. That's true.

In other words, as hair dyes using baladiphenylenediamine have become more popular, as if in proportion to this, some people have developed allergic reactions, but even though they are natural dyes, henna leaves, It has been found that sufficient consideration must be given to this point when using leaf extracts for hair dyes.

Therefore, the problem of the present inventors is to use henna leaves or
In order to use its extracts, we have been conducting intensive research to find the causative agent and to develop removal methods.

However, the causative substance has not yet been identified.
Therefore, the inventors of the present invention began experiments to find a way to solve this problem by determining what means should be used to ensure greater safety.

That is, the present invention, when using henna leaves, henna leaf extracts, and laucon in hair preparations, determines the concentration that is negative in an application test using animals by the maximization method, and determines the concentration to be used. As specifically described below, even when used on the scalp or skin, it is possible to suppress the development of allergies, and it is also possible to prevent dandruff and itching. They found that it is possible to use it continuously for a long period of time, and that it can be expected to prevent premature baldness and hair loss.

[2] Structure of the Invention The present invention provides that when henna leaves, henna leaf extracts, or lauson are blended into hair preparations, they are undone and 0.0
It is specified in the range of 17% or more and 0.035% or less.

In order to specifically disclose the present invention, the present invention will be described in detail based on experimental examples, examples, etc.

(1) Henna leaf end Henna leaves (leaves such as Lawsonia ainelmis that have been dried in the shade) have been used as hair dyes and dyes since ancient times after being crushed and pulverized. When freshly picked raw leaves (iook) are dried in the shade and crushed into a fine powder, a yield of about 20-35 kg can be obtained.

This fine powder has a yellow-green color, a slightly peculiar odor, a slightly bitter taste, and a pH of 1.0-20 when suspended in water.
0) is around 4.7.

Some Lawson contains 0.5 to 1.5%, but the values according to the six quality standards are not constant, as shown in Boshin (Table 1).

"Table 1, Summary of Lawson fine powder (Table 1 Test method notes) (A) Lawson confirmation test method 1 g of fine henna leaf powder 50 ml of 20% sodium carbonate solution
After being immersed in water for 2 to 4 hours, it is centrifuged, and the supernatant is made acidic with salt and extracted with chloroform three times. These three equal parts of the chloroform layer were mixed, an appropriate amount of anhydrous sodium sulfate was added, filtration was performed, and the filtrate was concentrated under reduced pressure, and mixed with methanol and 1:1 chloroform (1:1).
) Dissolve the mixture in 1 me and develop with 12-15 cIT+ by thin layer chromatography.

Support is silica gel 0% aluminum oxide = 1 = 1, layer is 0.31 m, activation is 140 ° C. for 4 hours, developing solvent is ethyl acetate, methanol, 5N-ammonium hydroxide-6
0:15:5° (B) Lawson's Quantification Weigh accurately 10 g of fine henna powder, immerse it in a 20% reconstituted sodium acid aqueous solution 100+1111 for 24 hours, centrifuge it, and collect the supernatant liquid. Repeat the above immersion until the supernatant loses its orange color, take the supernatant, combine the supernatants, add salt to make it Lidomus strict,
Extract several times with chloroform, separate the chloroform layer, add an appropriate amount of anhydrous sodium sulfate, filter through filter paper, and wash the used filter paper 2 to 3 times with a small amount of chloroform. After combining these filtrates, they were concentrated to dryness under reduced pressure, and methanol and chloroform were mixed at 1=
1, take 25 ml of the solution, and develop 25 μP of this solution by thin layer chromatography (same procedure as in test method A above).

Next, scrape off the Lauson part with Miku D Spadel,
Dissolved in 20% sodium R acid aqueous solution 1011111,
Filter through filter paper (Toyo Roshi & 5C).

The absorbance of this filtrate at a wavelength of 455n- is measured using a photoelectric colorimeter. In addition, as a blind test, thin layer parts of other parts were similarly scraped off and 20% sodium carbonate 101
After mixing 1Il, it is filtered through a filter paper and the filtrate is used.

Calculation formula: The calibration curve for quantitative determination is as shown in Figure 1, but to create it, after recrystallizing commercially available reagent grade Lawson, the same procedure as in the quantitative method is used. This was done by In other words, an absorbance of 0.28 corresponds to 2ooI1g of Lauson.

(2) The hair dyeing component contained in lauson or lauson-containing extract henna leaves is a naphthoquinone-based component, and these components are characterized by being eluted only in a very small amount in the water system alone. It is.

Therefore, when extracting extracts containing naphthoquinone components such as lounge or lounge itself, basically follow the procedures shown in the above-mentioned lounge confirmation test method and quantitative test method. , you can use a tataki table.

For example, by the method shown in Boshin (Table 2), a crude extract containing mainly lounge can be obtained, and for further purification,
On the acidic side, it is possible to easily separate the lounge using, for example, chloroform.

In Table 2, henna leaves are used after cutting (shredded), and the henna leaves are added to the extraction solvent combination (mixture), thereby reducing the lounge content in the immersion-extracted solution. The content was determined by liquid chromatography, and as a solvent for crude extraction, crude extraction methods using sodium charcoal, solution, sodium citrate solution, etc. are particularly good.

In addition, for purification, for example, based on the obtained immersion liquid, the pH of the system is made acidic using hydrochloric acid, etc., and further treatment with chloroform etc.
Gradually, a highly purified lounge-based extract is obtained. However, as shown in Table 2, extraction with immediate chloroform causes a decrease in yield from the beginning.

In addition, a crude extract (immersion solution) using ethanol has a strong green color, contains little naphthoquinone pigments, and contains a large amount of chlorophyll.

When lounge is finally purified from henna leaves, approximately 15±8 g is obtained from 1 kg of dried (dried in the shade) leaves.

(3) Amount of compounding amount that exhibits hair dyeing action When used in hair dyes, it should be contained in the suspension or extraction solution at least 0.3% and 0.3% or more in the suspension or extraction solution when converted to the amount of Lauson. A solution of about 5 to 1.0% is desirable as the amount for dyeing.

Therefore, the crude extracts shown in Examples (3) and (4) in Table 2 are used after removing the organic solvent and then concentrating or diluting them to these concentrations. or,
When using henna leaves themselves, take an appropriate amount of finely ground powder, do not add it to the water alone, and add sodium carbonate,
It is best to use it by suspending it in an aqueous solution such as sodium citrate.

Furthermore, when using the lounge or extract,
In water alone, it is poorly soluble, so 0.5
~1.0%, therefore, for example, in solutions of low concentrations of sodium citrate or sodium carbonate, as shown in examples (5) to (6) in Table 2. It is better to use it as

Next, we started a series of tests based on these henna leaves, their extracts, and purified lounge to specifically evaluate the safety of hair dyes and other products on the skin.

As a result, a very mild erythema was observed in the contact allergy test, although the conclusion was not completely positive.

1-In other words, as test solutions used in the experiment, in the prepackaging (Table 2), (1) used the crude extract as it was, and the other (2)
The crude extracts shown in the examples of ~(6) were obtained by removing the solvents used for each extraction (soaking) under reduced pressure, and then using chloroform.

The purified lounge obtained by extraction on the acidic side was dissolved in an aqueous solution and used as a sample for a patch test.In the experiment, the purity was quantitatively determined to be 99.8 as Lauson. % or more, as Lawson 9
1 group of guinea pigs, 10
The experiment was conducted using animals.

The results showed that, regardless of the degree of purification (purity) of Lauson, in the contact allergy test, only erythema occurred in 0 to 1 out of 10 animals in one group, and 1 in two groups.
It was found that extremely mild erythema (frequency: 1) was observed.

The inventors of the present invention placed importance on these results, temporarily halted development of the product as a hair dye, and searched for inflammatory substances as well as measures to prevent its reactivity.

In other words, the purity was close to 100%, which was confirmed even in Lawson itself, so the application to hair dye was abandoned.

When estimating one of the causative substances, the Lauson structure is as shown below, and its active group is thought to be in OH, which influences the expression of pharmacological action and the induction of erythema. I have come to the conclusion that they may be hatching.

In other words, in order to use it as a practical hair dye that takes advantage of its hair dyeing action, the preparation (prescription) must contain 0.3% Lawson.
Considering that the above is not necessary and that it is desirable to formulate a formulation containing about 0.5 to 1.0%, it became necessary to reexamine safety.

(Structural formula of Lauson) %Formula% In other words, contact allergic reactions to Lawson itself are extremely mild, but henna leaves also contain various pigments or substances that are involved in coloring ability. Conceivable. In addition, it is assumed that crude extracts containing these substances may exhibit an even stronger reaction to C, or may even be weakened, so taking these points into consideration,
The test was conducted again, but very mild erythema was observed in each case without affecting the degree of purification.

Therefore, the present inventors investigated the physiological activity of each drug.
It is believed that the OH group in the structure of various naphthoquinones plays an important role, and for example, in the expression of pharmacological activity, binding with amino acid residues derived from proteins in the body is the cause of erythema. I assumed that this might be the case, so I added a few substances to the formulation (preparation) and tried it out further. For example, cyclodextrin, water-soluble elastin,
After adding bovine serum-derived albumin etc. to each solution, a contact allergy test was attempted again, but the results were extremely mild (frequency: 1) in about 1 out of 10 animals per group. ) reactions were observed.

In these solutions, amino acid residues derived from the added protein react with the OH groups of Lauson, resulting in the compound being changed or becoming an inclusion compound with cyclodextrin. Although it is difficult to say that there is, no change was observed in the number of cases occurring, and it has not been possible to suppress them.

As a precaution, aqueous solutions of cyclodextrin, water-soluble elastin, and bovine serum-derived albumin added to the system were prepared in advance, and the presence or absence of their expression was confirmed and compared. However, no abnormal reactions such as erythema were observed in these cases, and the evaluation frequency was zero.

(Commentary on contact allergy test method) Using female Hartley guinea pigs weighing around 330 g, 10 animals per group, maximization method (Reference source: New toxicity test and safety evaluation published by Soft Science) and Takase's method (Fragrance Journal Nc14 19
75), first, the drug (test solution) was emulsified by mixing with Floyd complex adjuvant and IC.
A sarcastic injection was given to the M part of the shaved cell e-soto. 1 week later,
Newly shear sp gg, 3 x 2 cm (7)
A test solution of 0°5 was dropped onto a lint cloth, and then a polyethylene film was used to bandage it and fixed for 48 hours. Two weeks later, it was used for a provocation test.

After the above pretreatment, shave the flank of the guinea pig and
- The test solution was dropped onto Beiersdorf's Plasgoo patch, and it was firmly stuck for 24 hours.

Judgments were made based on the evaluation criteria in Table 3, using erythema and edema as indicators immediately after application (dose at zero hour) and 24 hours later.

1 Table 3, Contact allergy test evaluation criteria (4) Safety of henna leaves or its extract solution In view of the current situation where these products are being used in an increasing number of cases, we believe that it is necessary to take immediate measures to determine the safety of these products on the skin, and based on the test method described above,
For the time being, in order to ensure safety, we decided to estimate the amount of henna extract that would have no effect even if added to various hair preparations based on the relationship with the amount of lauren contained in each type of henna extract.

As a result, according to Boshin (Table 4), in the extract obtained by combining each solvent, the upper limit is 0.035% when converted to Lauson L Yu, and if it is less than this, there is of course edema. It was confirmed that there was no erythema.

However, this amount represents the upper limit of the amount that can be added when using henna leaf extract or lauson in hair preparations, and is an important point in formulating products to ensure safety. I can say that.

With this method, it is impossible to utilize the hair dye as a hair dye that has been passed down since ancient times.

In other words, if you want to get the full hair dye effect in -degrees,
At least, it is necessary to use a solution with a concentration of at least 0.5%, but in such cases, please be aware that continued use may, in rare cases, cause mild contact allergy. It can be said that it is necessary.

In other words, the idea that ingredients derived from natural products are equally safe suggests that sufficient caution should be taken when it comes to substances that have a hair dyeing effect, just as with other synthetic dyes.

In applying henna leaves as a hair dye, it was thought that a restraint was needed in the same way as rose phenylene diamine.

Table 4: Contact allergy effect of henna leaf extract (guinea pigs) (5) Human use test In the previous section, when formulating henna leaf or henna leaf extract, lounge, the concentration that can cut down on the allergy-inducing ability to the skin is determined. However, in actual hair preparations, when converted to the amount of Lauson, there is a concentration of 0.035% or less, and is there any merit in using it?

In other words, as stated in the previous section, when applying as a hair dye, if 0.035% or less is defined as a safe and harmless amount, then a hair dye with this additive amount cannot dye hair at all. It will fall short.

Therefore, the inventors of the present invention actually formulated a safe amount for hair (including the scalp), even though it is not suitable as a hair dye, and tested the use of the product to prevent the occurrence of rashes and itching. , I decided to try using it with this in mind to see if there were any other effects.

In the formulation of known hair tonics, hair liquids, hair rinses, and hair shampoos, each extract obtained by steps (1) to (6) in Table 2 above, including Lounge, was used. In each formulation, we varied the amount added, mainly 0.03% as Raushi 1, and at the same time made various hair products as prototypes, including those with no additives, and monitored the application. I chose and had them use it.

Boshin (Table 5) uses a hair tonic containing Lounge in its formulation, targeting healthy men who wash their hair before taking a bath, sprinkle it on their hair, and then thoroughly rub it into their scalp. Here are the results when I used it in the same way as pine surge.

In addition, please use it continuously for 3 months, and if you experience itching, worsening of dandruff, or rash on your scalp or other areas, stop using it and consult a doctor for diagnosis and treatment. On the condition that they comply with the rules, each monitor was given 2 bottles of 300 pieces in a 7-piece brass container for cosmetics, and asked to report on the progress of using the toilet.After 3 months,
The survey was conducted by asking each monitor to respond.

During the period, one person discontinued use without additives.
However, upon discontinuation of use, each symptom disappeared and did not require medical diagnosis or treatment.

We provided a notes section on the answer sheet and asked each person to write down any points they noticed, but the answers ranged from 0.025 to 0.
．． 7 out of 10 people who used Bare Tonic, which contains 040% hair tonic, found that it was better than the hair tonic they had previously used. Three monitors responded that they were able to control the situation better.

rTable 5.

Use history of Art Nink to include lounge (Table 5)
Based on the results from the monitor, it has no effect (effect) as a hair dye, but there has been no increase in itching, dandruff, or rashes, which were the most worrying, and the upper limit set by the present invention has been found. It was estimated that there would be no problem at 0.035% or less.

In addition, it is reassuring to know that many respondents said that it suppresses itching and dandruff when it contains 0.025 to 0.040%. The results showed that the number of respondents who felt that the product had an effective effect increased as its concentration (amount) increased. In this respect, it seemed that there were sufficient benefits for hair products.

In other words, existing drugs that prevent itching and dandruff,
Also, among the drugs that promote hair growth, hair growth, and hair growth, there are appropriate amounts for each. For example, resorcinol, cantharis tincture, benzyl nicotinate, zinc pyrithione, etc., if used in appropriate amounts,
In addition to preventing dandruff and itching, it also has the effect of promoting hair growth. However, if you increase the amount with too much expectation, it will actually increase dandruff and itching, and as a result, it will suppress hair growth, hair nourishment, and hair growth promoting effects.

Therefore, in the case of resorcinol, cantharis tincture, etc., when using these drugs, upper limit amounts for each are specified in cosmetics due to safety considerations such as skin irritation. For example, the standard for resorcinol is 0.1%, but according to batch tests on animals, this concentration is still far from safe.

In other words, according to the research results of the present inventors' past animal experiments, etc., among the substances that have a hair growth promoting effect, the stronger the effect, the more it causes various irritations to the skin (-secondary irritation, phototoxicity, etc.). (reactions such as contact allergies) are often positive.

When these substances are used in extremely low amounts (concentrations), itching and dandruff on the scalp can be suppressed.

That is, by using degree A, which is negative for stimulation in animal experiments, dandruff and itching can be suppressed, and hair growth, hair growth, and hair loss can be prevented.

Therefore, for example, when using commercially available hair tonics or hair growth agents that contain resorcinol or benzyl nicotinate, it is often seen that dandruff and itching increase. Many of the products are
When hair growth tests are conducted on animals, they often show a strong effect on promoting hair growth. In other words,
Many of those with strong stimulating effects also have hair growth promoting effects.

If the concentration used is appropriate, dandruff and itching can be suppressed, and it is presumed that hair growth and the occurrence of dandruff and itching are closely related.

(6) Discussion regarding hair growth promoting effect The present inventors noted that in the data shown in Table 5 above, there were cases in which respondents answered that hair growth was accelerated.

In other words, it has been confirmed that the occurrence of dandruff and itching can be suppressed by using Heartonic at an extremely small concentration, which was negative for contact allergy in animal experiments, taking into consideration safety for the skin. This raises the question of whether such a small amount of formulation can actually promote hair growth. We will discuss the potential benefits of using it as a hair growth agent, considering its effectiveness as a hair (hair) promoter or scalp dandruff and itch prevention agent, as well as its side effects (increase in dandruff and itch). I tried it.

As a result, the present inventors have already disclosed, from a completely different angle, the use of Lawson et al. It has been reported that it is useful in treating baldness and hair loss.

In other words, regarding the results shown in Table 5 above, one of the objectives of the present inventors was development research centered on the evaluation of safety to humans. Initially, it was thought that there would be no effect (benefits) when using the specified low concentration additive amount, even though safety could be ensured.

However, as shown in the front cover (Table 5), the results obtained exceeded the expectations of the inventors, especially regarding the example of hair growth. I became particularly interested in the fact that it had been found to have a similar inhibitory effect on testosterone 5α-reductase.

Therefore, we revisited and considered the relationship between the concentration in the system having an inhibitory effect in the test in the above-mentioned publication. In other words, when comparing the amount of solution in the reaction system and the amount of Lawson added in the system, the amount of solution in the reaction system is about 6.5 mounds, and 5μ of Lawson is added to this. At this time, the inhibition rate of testosterone 5α-reductase was 95%.

In other words, if we consider this as the concentration in a specific formulation, that concentration is obtained in a solution containing about 0.077%.

Therefore, the answers regarding hair growth obtained in Table 5 can be considered to be one of the reliable effects.

When we consider the mechanism behind the use of Lauson and its effects, we find that in small doses, it is involved in the production and maintenance of testosterone, inhibits excess dihydrotestosterone, promotes hair growth, and reduces dandruff and itching. It was determined that this may be suppressing the

(3) Effects of the Invention The present invention has successfully developed henna leaves, henna leaf extracts, and laucon by determining the amount of henna leaves, henna leaf extracts, and laucon in a hair preparation from the viewpoint of safety for the skin. However,
No other example like this has ever been found.

The present inventors envisioned that henna leaves, henna leaf extracts, and laucon would be used in cosmetics and quasi-drugs in Japan in the future, and sought to formulate them into safer formulations. Although we have continued to carry out preliminary investigations and research, we have decided to abandon the application (use) as a hair dye for the time being in order to ensure safety.

However, without giving up on this, we have been seeking safety evaluation, and when using it in hair products, there is no problem if used as Lawson in the range of 0.017 to 0.035%. We have discovered that it can be expected to have the effect of preventing dandruff and itching, as well as promoting hair growth (hair growth, hair growth).

This is the greatest effect of the present invention. Furthermore, it is believed that the present invention will serve as a trigger for the development of various measures to prevent contact allergies in future applications as hair dyes or hair growth agents. To this end, for example, it may be prescribed (formulated) in combination with various known substances, such as anti-staminic agents, anti-inflammatory agents, adrenocortical hormone reducers, and other known anti-allergic agents. In addition, in that case, one effective method would be to formulate a formulation by combining natural plant-derived extracts, such as licorice extract (glycyrrhizin) and chamomile extract (azulene). Although it is difficult to predict, I would like to look forward to the results of future research on these points.

Claims (1)

[Claims] (1) When quantifying the content of Lawson, 0.017% to 0.01%.
henna leaf powder, characterized in that it is in the range of 0.035%;
A hair preparation containing either henna leaf extract or lauson.