JPH02255077A - Culture bag - Google Patents

Abstract

PURPOSE:To obtain a culture bag capable of storing a medium over a long period in a state poured into the bag by forming a double-walled bag with a gas-permeable sheet and gas-impermeable sheet, attaching a protector at the month of the bag and connecting a pouring tube to the protector. CONSTITUTION:The objective culture bag 1 is produced by attaching a rubber button part 3 for the introduction and sampling of culture cells, a pouring tube 4 for the pouring of a medium and a discharging port 5 for the discharge of the medium and culture cells to the mouth part of a bag 2 formed with a sheet having a double-wall structure. An aseptic filter 7 is placed in the middle of the pouring tube 4.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention is a culture bag used for cell culture, and is suitable for culturing cells and microorganisms, whether for research or industrial production. It is used on occasion.

[Prior Art and Problems of the Prior Art] FIG. 5 shows a schematic diagram of a culture bag 30 currently used as a culture container.

The sheet constituting the culture bag 31 has good gas permeability so that oxygen and the like can be supplied to the cultured cells.

However, since the gas permeability is good, if the culture medium is injected into the bag body 31, the carbon dioxide gas added to maintain the pH of the culture medium will diffuse to the outside of the bag body through the sheet. etc., and the medium could no longer be used for cell culture.

In this way, it is difficult to inject the culture medium into the culture bag through the injection tube 32 and store it as it is for a long period of time.

In addition, a culture medium bag made of a gas-impermeable sheet can maintain pH etc. at a constant value because oxygen, carbon dioxide, etc. cannot pass through the sheet even if the culture medium is injected into the bag and stored. Although it is possible to store cells for a long period of time, it is not possible to supply oxygen, etc. necessary for cell culture through the sheet, making it very difficult to culture cells.

[Means for Solving the Problems 1] The present invention provides a culture bag 1 for culturing cells, which comprises a steel main body 2 formed of a double-layered sheet and a plurality of openings 5 formed on the top thereof. A rubber button part 3 and an injection tube 4 are attached, and a sterilization filter 7 is placed in the middle of the injection tube 4.
A culture bag 1 formed of a gas-permeable sheet and a gas-impermeable sheet is provided.

[Function] By covering a culture bag made of a gas-permeable sheet with a gas-impermeable sheet, the culture medium can be stored for a long time while being injected into the culture bag, and the outer gas-impermeable sheet can be removed to prevent gas permeation. By exposing the adhesive sheet, it can be used as a culture bag suitable for culturing cells.

[Example] FIG. 1 shows a schematic diagram of a culture bag 1 of the present invention.

The culture bag 1 basically has a bag-like main body 2 formed of a double-layered sheet, a rubber button part 3 on the top of the bag-like main body 2 for injecting or sampling cultured cells, and an injection button used for injecting a culture medium. It is composed of a tube 4 and an opening 5 for leading out the culture medium and cultured cells.

A plurality of mouth parts 5 are formed, and all the mouth parts 5 are covered with a protector 6.

Further, a sterilization filter 7 is provided in the middle of the injection tube 4.

The above-mentioned double-structured sheet means that one side is a gas-permeable sheet made of ethylene-vinyl acetate copolymer, polyethylene, polyvinyl chloride, polystyrene, etc., and the other side is a gas-permeable sheet made of ethylene-vinyl acetate copolymer, polyethylene, polyvinyl chloride, polystyrene, etc.
A sheet formed by pressure adhesion or gluing of a gas-impermeable sheet made of vinyl alcohol, polyvinylidene chloride, polyamide, etc., or a sheet formed by covering a gas-permeable sheet with a gas-impermeable sheet.

Next, a method of using the present invention will be explained in detail using FIG. 2.

First, the culture medium 8 in the container 12 is injected into the bag-like main body 2 via the injection tube 4 by the pump 13.

At this time, bacteria and microorganisms mixed in the culture medium are removed by a sterilization filter 7 provided in the middle of the injection tube 4.

Therefore, no contamination occurs due to the injection of the medium 8.

After that, as shown in FIG.
Since the outside is covered with a gas-impermeable sheet 10, the PH
Since the values of , etc. can be maintained constant, the culture medium can be stored for a long period of time.

In addition, when culturing cells, etc. using the culture bag, by removing the outer gas-impermeable sheet of the double-layered sheet, the gas-permeable sheet inside the bag can be removed as shown in Figure 4. 11 is exposed, and cells can be easily cultured simply by injecting cultured cells from the rubber button part 3.

Note that, as described above, the double-layered sheet is formed by pressure adhesion or gluing of non-adhesive resin, so the outer sheet can be easily removed by simply rubbing the sheet lightly.

In addition, for example, as shown in FIG. 5, a culture bag made of a gas-permeable sheet is placed in a bag 21 made of a gas-impermeable sheet, and the oxygen inside the bag is removed using a deoxidizing material. You can get the same effect.

[Effects] As explained above, the present invention does not cause contamination when injecting the culture medium into the bag, and can store the culture medium without changing the pH etc. for a long period of time. By simply removing the covering gas-impermeable sheet, culturing can be carried out quickly and easily.

This is an excellent invention that has the following effects.

[BRIEF DESCRIPTION OF THE DRAWINGS] Fig. 1 is a schematic diagram of the culture bag 1 of the present invention, Fig. 2 is a schematic diagram of injecting a medium into the culture bag of the present invention, and Fig. 3 is a schematic diagram of the culture bag 1 of the present invention. FIG. 4 is a diagram of the state of use when culturing cells using a culture bag, FIG. 5 is a schematic diagram of another embodiment of the present invention, and FIG. 6 is a conventional A schematic diagram of a culture bag is shown. In the figure, l is a culture bag, 2 is a bag-like main body, 3 is a rubber button part, 4 is an injection tube, 5 is a mouth part, 6 is a protector,
7 indicates a sterile filter. Patent applicant: Kawasumi Chemical Industry Co., Ltd.

Claims (1)

[Claims] A culture bag for culturing cells, which has a bag-like main body formed of a double-layered sheet, a plurality of openings, a rubber button part, and an injection tube attached to the upper part of the bag, and a sterilized part in the middle of the injection tube. 1. A culture bag, wherein a filter is arranged, and the double-structured sheet is formed of a gas-permeable sheet and a gas-impermeable sheet.