JPS59206015A - Filter apparatus comprising plastics - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention relates to a transparent filter canister-shaped casing with a filling height marking line formed in the upper part and the inlet pipe and in a number of chamber walls for the filling volume to be measured. and a filtration device consisting of a seven-chambered plastic filtration canister having a filtration medium and a casing lower part joined in a leak-tight manner with an upper part having an outlet.

PRIOR ART According to the state of the art as described in German Patent No. 23 34 375 and German Utility Model Registration No. 8 014 162, a casing of this type, for example for concentrating a liquid medium, is known to It is used for the purpose of concentrating this aqueous solution as much as possible when examined from the point of view. In order to control the various concentration levels optically from the outside, markings printed on the housing wall or dividing markings inserted or protruding directly into the plastic are also molded. This marker actually simplifies optical control, but due to its placement and size it limits direct observation from the shortest distance. However, in the case of common laboratory studies, the user has to monitor a large number of sets of casing units of this type, which means that the user has to monitor a large number of sets of casing units of this type, which can be used at different points of the type of arrangement and at different time sequences of the enrichment process. This represents significant research costs for users and supervisors.

Similarly, casing and filter units of this type are used for sterile filtration and sterility testing of liquid media. Devices and applications of this kind have already been described in US Pat. No. 3,448,911, DE 2549835 and DE 3012085. A closed cylindrical filtration canister made of transparent plastic and having an inlet and an outlet is filled under sterile conditions with the medium to be filtered, a certain filling height being selected depending on the preconcentration of this medium. , this filling height again corresponds to a constant volume and gives rise to negative inferences about the Keim content or impurities of the liquid when testing residues remaining on the filtration media quantitatively and qualitatively in the laboratory. . In order to detect microorganisms that may remain on the filtration media, generally sterile, closed filtration canisters are each charged with a culture medium and the sample is subsequently directly filtered under aerobic or anaerobic conditions. Ovulated in a canister, in which case it is desirable to keep the surface area of the liquid that absorbs oxygen as large as possible during aerobic ovulation and as small as possible during anaerobic ovulation. Due to the shaped filtration canister, the liquid surface area is the same during aerobic and anaerobic ovulation.

How much microorganisms and embryos (Ke
im) is carried out separately in a number of filtration canisters at the same time using different culture media under different conditions in order to obtain better control and reporting of the presence of im) in the sample to be tested.

In the case of the above-mentioned filtration device according to the present invention (West German utility model registration application no. 8108010.7), the upper part of the filtration canister-shaped casing has an outer diameter of about 50 mm and a height of 90 mm.
has. The filling height mark runs in the direction of the longitudinal axis of the filtration canister and may be placed as a mark block on the front half of the side wall, or on the back half of the side wall, or
Or it is placed on the entire side wall. Two or three such identical filtration devices can be combined via a hose splitter and a common puncture cannula to turn into a sales unit and a usage unit, so that the user can sequentially connect the filtration canisters to the suction device. When installing on top, care must be taken to ensure that the filling height mark is visible from the front side, which is not always guaranteed due to distortions in the hose system. .

Problem to be Solved by the Invention It is an object of the invention to avoid defining individual circumferential sections in the circumferential direction of a transparent filtration canister, which are optically well visible and capable of defining the contents of the upper part of the casing. It is an object of the present invention to obtain in a simple manner a filter device with a filling height marking which divides the material in terms of volume with free liquid surfaces of various sizes. Additionally, efforts are made to optimize handling and strength as well as other functionalities such as intake and exhaust.

Means for solving the problem: A transparent filtration canister - the upper part of which consists of a four-step hollow truncated cone, the steps of which form a surrounding filling height mark, in this case facing a wide base. The first truncated conical stage below the filter has a filling capacity of approximately 50 m1 from the filter face of the filter to the 10th stage layer, and the second and first truncated conical stages together have a filling capacity of approximately 75 sq. 3. has a capacity; The second and first truncated conical steps together have approximately 100
m1 or optionally about 110 m1, with a filling capacity of the last truncated conical step up to the casing lid of about 10 m1.
~20 m1, or a) a transparent casing in the form of a filter canister, the upper part of which has the form of a four-stage hollow truncated cone; The tiers form a surrounding filling height mark, in which case the lower first truncated conical tier opposite the wide base covers approximately 5 Qml from the filter surface of the lower filter to the first tier. 3. having a filling capacity, the second and first frustoconical stages together having a filling capacity of approximately 75 m1; The second and first truncated conical steps together have approximately 100
mij or optionally has a filling capacity of approximately 110 m/, with a filling capacity of the last frustoconical stage up to the casing lid of approximately 10
~20 m1; b) The top of the filtration canister in the casing lid is approximately 5 ml above the highest filling height mark inside the casing.
a first inlet pipe projecting up to a filling height of , the first inlet pipe forming a hose connection pipe for a filling hose that can be rigidly connected to a hose connection pipe on the outside of the filtration canister top; and C) a filtration canister with a permeable filter support member having a closable outlet tube and forming a small casing chamber, the lower part of which supports a hydrophilic filter membrane with a pore size of 0.2μ to 0.45μ; The filtration membrane has a surrounding hydrophobic edge, the lower edge of the top of the filtration canister;
This hydrophobic edge between the upper edge of the lower part and the upper casing part serves as a selectively permeable separating wall sandwiched in a sandwich-like, leaktight manner between the surrounding flange part and the web part. is separated from the lower casing part, in which case one of the two edges of the two casing parts axially overlaps the outer edge of the other casing part and optionally additionally covers the sandwich of the lower filter. The axially and possibly radially overlying edges of one casing part overlap in such a way that the water-tight edges are sealed off from the atmosphere surrounding the casing in a gas-tight and leaktight manner. , welded, welded or glued to other casing parts, d) an opening in which a small filter is integrated into the casing lid, which is arranged as an intake/exhaust filter in the casing lid; It consists of a flat hydrophobic filter engraving with a pore size of 0.2μ to 0.45μ covering the mounted casing, which has on one side at the edge a filter support member, a free space and a connecting tube. It is sandwiched between the upper part and the casing lid, which is formed on the other side as a casing lower part, in a leak-tight manner at the edges of the lid opening, in which case the two casing parts are permanently leak-tight at the edges. combined, the unobstructed filtration area of the flat filter engraving approximately corresponds to the area of the lid opening, e) the filtration canister - a puncture cannula or a coupling adapter whose upper filling hose has a removable protective tube on its side; and optionally together with other sterile filtration devices via a flow divider (branching section) located downstream after the puncture cannula or coupling adapter. achieved by a filtration device made of plastic, characterized in that it is held in a sealed magazine, combined and sterile, in a closed, sterile use unit in the form of a double kit, or a) The upper part of the transparent casing in the form of a filter canister has the form of a four-stage hollow truncated cone, the tiers of which form a surrounding (filling height mark), in this case facing a wide base. a first truncated conical stage having a filling volume of about 50 m from the filter surface of the lower filter to the first stage layer;
The second and first frustoconical stages together have a filling capacity of approximately 75 cm; 3. The second and first truncated conical steps together have approximately 100
ml or optionally about 110 +++ l, with a filling capacity of the last truncated conical stage up to the casing lid of about 10
~20 m1; b) The top of the filtration canister in the casing lid is approximately 5 ml above the highest filling height mark inside the casing.
a first inlet pipe projecting up to a filling height of , the first inlet pipe forming a hose connection pipe for a filling hose that can be rigidly connected to a hose connection pipe on the outside of the filtration canister top; and C) the second connecting pipe arranged in the casing lid has a filter surface supported on both sides of the casing lid as an intake-exhaust filter with a pore size of 02μ to 0.45μ on the outside of the filtration canister top. It has a Luer-type cone and/or thread for a small filter projecting outwardly from the casing lid, which is connected in a leak-tight manner to the second connecting pipe, but is not necessary. d) a permeable filter support having a closable outlet pipe and forming a small casing chamber; A filtration canister with a lower part supporting a hydrophilic filtration membrane with a pore size of 02μ to 0.45μ, the hydrophilic filtration membrane having a surrounding hydrophobic edge, a lower edge of the filtration canister upper part and a filtration canister
This hydrophobic edge between the upper edge of the lower part serves as a selectively permeable separating wall between the surrounding flange part and the web part in a sandwich-like, leaktight manner. Separated from the lower casing part, in which case one of the two edges of the two casing parts axially overlies the outer edge of the other casing part and, if necessary, additionally, the sandwich of the lower filter. radially overlapping the casing in such a way that the water-tight edges are sealed off from the atmosphere surrounding the casing in a gas-tight and leak-tight manner, in which case 1
the axially and possibly radially overlapping edges of one casing part are welded or glued to the other casing part; e) the filtration canister - the upper filling hose has removable protection on that side; A flow distributor (
closed in the form of a double or multifold kit with the above characteristics together with other sterile filtration devices via branch parts),
This is achieved by a filtration device made of plastic, characterized in that it is combined in a sterile unit of use and held in a sterile, hermetically sealed magazine, or a) a transparent casing top in the form of a filtration canister; has the shape of a four-step hollow truncated cone, and the steps of this hollow truncated cone form a surrounding filling height gauge, in which case the lower first truncated cone step facing the wide base is The filter has a filling capacity of about i5Qml from the filter surface to the first stage layer, and the second and first frustoconical stages together have a filling capacity of about 75ml.
It has a filling capacity of 3. The second and first truncated conical steps together have approximately 100
m1 or optionally a filling capacity of approximately 110111 g, with a filling capacity of the last truncated conical stage up to the casing lid of approximately 10 g.
~20 ml, b) the filtration canister in the casing lid - the upper part of the filtration canister is within the casing above the highest filling height mark, approximately 5 ml at most
a first inlet pipe projecting up to a filling height of , the first inlet pipe forming a hose connection pipe for a filling hose that can be rigidly connected to a hose connection pipe on the outside of the filtration canister top; and C) a filtration canister with a permeable filter support member having a closable outlet tube and forming a small casing chamber, the lower part of which supports a hydrophilic filter membrane with a pore size of 02μ to 0.45μ; The hydrophobic membrane has a surrounding hydrophobic edge between the lower edge of the filtration canister top and the upper edge of the filtration canister bottom. The upper casing part is separated from the lower casing part as a sandwich-like, leak-tight and selectively permeable separating wall, in which case one of the two edges of the two casing parts is connected to the other. axially over the outer edge of the casing part and optionally additionally so that the sandwiched water-tight edge of the lower filter is sealed off from the atmosphere surrounding the casing in a gas-tight and leak-tight manner. d) a casing lid with free space, in which case the axially and possibly radially overlapping edges of one casing part are welded or glued to the other casing part; d) a casing lid with free space; A small filter placed in the casing lid as an intake/exhaust filter with a filter support integrated therein supports a flat hydrophobic filter engraving with a pore size of 0.2 μm to 0.45 μm on the side of the filtration canister. , this flat hydrophobic filter engraving forms a leak-tight seal between the mounted casing upper part with the filter support element, free space and connecting tube on one side on the edge side and the casing lid formed as the casing lower part on the other side. in which case the two casing parts are permanently connected in a leak-tight manner at the edges, the flat filter engraving rests on one or the other filter support element depending on the direction of the pressure gradient, and dl ) The filter support member in the lower part of the casing forms a flat surface and a closed surface, the free space of the filter support member comprising only one annular passage of at least 2 mm width within the periphery of the flat filter engraving; formed by at least one blocking channel, which blocking channel joins a collection channel guiding the annular channel into the interior of the filtration canister, d
2) The blocking channel extends from 1 mm starting from the collection channel opening to 3 m with a filter diameter of 25 mm towards the annular channel.
d3) The bottom of the blocking passage is approximately 0.3 to 0.8 mm below the filter support surface within the collection passage and slopes downward and 11 to 16 mm towards the annular passage. d4) The opening of the collection passage is 2 mm long and 2 mm in diameter within the range of the filter support member at the bottom of the casing directly from the wide base facing the liquid reservoir inside the filtration canister. e) filtration canister - flow in which the upper filling hose terminates upstream of the puncture cannula or coupling adapter with a removable protective tube on its side and optionally located downstream behind the puncture cannula or coupling adapter; Sealed, combined and sterilized in a sterile use unit, closed in the form of a double or multifold kit with the characteristics described above together with other sterile filtration devices via a distributor (branching part) This is achieved by a filtration device made of plastic, characterized in that it is held in a magazine. Other preferred formations and optimizations include any one of claims 2 to 6 and claims 9 to 13.
It is described in any one of the following items or any one of the items 15 to 19.

Effects of the Invention The advantages of the above-mentioned casing configuration are that the filling height mark relative to the circumference of the filtration canister is uniformly well visible over the entire circumference, and that a relatively large filtration surface area within the casing base is achieved. During the free liquid surface area decreases stepwise towards the casing lid, thus the large surface area with a small filling height allows for good gas exchange during aerobic loading, and for anaerobic loading. It can be seen that with a particularly large filling height of 70 ml, only a small surface area is used, which makes pressure relief gas exchange or undesired oxygen uptake even more difficult. By possible reinforcing of the casing in the region of the filling height step and by loupe-shaped formation of the transition, the strength of the casing in the negative plane can be increased;
On the other hand, the optical control method can be improved within the filling height gauge. For filtration with vacuum loading and fertilization under aerobic conditions, the filter formation of the suction-exhaust filter at the top of the filtration canister is such that the liquid from the top of the filtration canister is mixed during filling or by hand with the culture medium. The situation is such that complete blockage of the hydrophobic filter membrane is impossible if it penetrates the lower surface of the hydrophobic filter membrane during the process.

When a canister-type filtration device with a sealed liquid and an integrated gas filter is carefully shaken, for example by conventional laboratory methods, in order to homogeneously mix the filtration canister contents, the filter support member, its free space The filling of the free space with liquid is also prevented by the inventively formed shape of the connection passage of the liquid reservoir to the gas space. However, if in reality under extreme conditions or due to improper handling of the filtration device liquid penetrates into the plane of the hydrophobic membrane, the membrane movable between the two filter supports will be forced to exchange pressure due to its pumping motion. serves to evacuate the free space of the filter support member below, so that exhaust and intake air is guaranteed even under extreme conditions.

DESCRIPTION OF THE PREFERRED EMBODIMENTS The idea of the invention is explained in detail in a number of embodiment examples and with reference to the accompanying drawings.

The filtration device comprises a filtration canister-shaped upper part 5 made of generally transparent plastic, a filtration canister having a permeable filtration support member 18 with a lower casing chamber 15.
It consists of a lower part 14 and a filter member 9 having a diameter of 50IIIrl sandwiched between the upper part 5 and the lower part 14 in the shape of a sandwich. The casing upper part or filter canister upper part 5 has the shape of a four-stage hollow cone, the steps having an inclination of approximately 1° forming surrounding filling height marks Ml, M2° M3; In this case a wide base or casing lower part or filter canister lower part! The lower first truncated conical step 1 that co-belongs to 4 is
The first truncated conical stage M1 from the filter surface of the filter 19
The second truncated conical stage and the first truncated conical stage together have a filling capacity of approximately 75 m1; The second and first truncated conical stages 1, 2.3 together have a filling capacity of approximately 100 mA' or optionally approximately 110 ml. The filling capacity of the last truncated conical stage 4 up to the casing lid 6 is approx.
It is 20-.

An inlet pipe 7 projecting from the casing lid 6 is arranged in contact with the inner wall of the casing of the uppermost truncated conical stage 4, the other end of this inlet pipe extending up to a filling height above the last filling height mark M3 of approx. 5 ml into the uppermost filling height stage 4, which is filled with the liquid to be filtered up to the third filling height mark M3. Mostly used as gas collection chambers. As a result, the liquid to be injected is
When this liquid leaves the filling hose 11 due to pump pressure, it runs along the inner wall of the filter canister without too strong a spray.

A further connecting tube 8 is arranged in the housing lid 6, which connects a sterile intake/exhaust filter 10 and a threaded thread 10 with a Luer cone 9 and connecting tube 8. “In this case,
The filtration surface of the hydrophobic filter 10' is a filtration canister.
The hydrophobic filter membrane 10' is located significantly higher than the respective highest filling height surface of the upper part 5, so that even when the liquid in the filtration canister upper part 5 is agitated, the hydrophobic filtration membrane 10' does not become wet and thus does not become clogged. There's nothing to do. Intake-exhaust filter 1
0 plug 12' can be sealed by plug 13. Such a small filter is clearly shown in FIG.

The inlet tube 7 is designed as a pipe connection for a filling hose 11 which terminates on its side with a puncture cannula 21 or with a connecting adapter customary in other experiments and with a removable protective tube 22. is provided. The filling hose 11 can be tightened at any desired position using a commercially available hose clamp 20. The filling hose 11 can be inserted into a roller hose pump, so that the filtration device is loaded with pressure, but instead of the hose pump the outlet pipe 16 is connected to the filtration canister lower part 14.
Can be connected to a vacuum source. Furthermore, the connecting tube 16 is closed with a closure cap 17 and forms an outlet for the filtrate.

As is clear from FIG. 2, the thick wall portion 50 differs from the embodiment shown in FIG.
are the diameters of the adjacent truncated conical steps Ml, M
2. This can be achieved by overlapping by 1 to 2 times the wall thickness within the range of M3. Together with the reinforcing effect within the region of this connection point, this can lead to a corset-like stability and reinforcement of relatively thin-walled filter canisters, and during the formation of a corresponding bulge. A magnifying glass effect also occurs, so that the filling height plane can be observed well.

In the special case of the sterility test, a hydrophilic filter membrane 19 with a hydrophobic edge 19' and which forms an inherent sealing area of the filter element 19 has proven effective. . 1st
Hydrophobic edge 19' as shown in FIGS.
are the filtration canister - upper part 5 and the filtration canister - lower part 1
4 and actually between opposing and surrounding flange portions 5', 14'. Also in order to guarantee the pressure stability of the filtration device in the longitudinal direction, one of the two edges 5'' of the two casing parts 5, 14 is axially attached to the outer edge 14' of the other casing part 14. The atmosphere surrounding the casing 5.14 overlies the casing 5.14 in such a way that the sandwiched hydrophobic edge 19' of the lower filter element 19 is air-tight and leak-tight, and optionally additionally to improve the pressure stability. The radially overlapping edge 5' of one casing part 5, in this case axially and optionally radially overlapping so as to be isolated from the other casing part 14, is welded or glued to the other casing part 14. There is.

This is one preferred method of attachment when the use of the filtration device is initially provided as a disposable item. In contrast to the flange formation shown in FIG. 2, the filtration canister lower part 14 has a surrounding flange, so that the filtration canister lower part 14 is dish-shaped.

Such a design is also provided if the upper part 5 and the lower part 14 have to be removably connected by means of an elastic ξ-kin ring in a bayonet or screw connection. Even for such filter devices, the stepped truncated conical formation according to the invention with surrounding filling height gauges provides the advantages mentioned above.

A connecting tube with a small filter 10 connected to the casing connection 8, which is fixed as an intake-exhaust filter, but can be removed if necessary, is preferably connected with a separate small filter 10 after the isolated newborn eggs. Samples for quantitative/qualitative measurements can be removed by syringe or pipette through 8 and tested.

According to FIGS. 3 and 4, gas inlet pipe/gas outlet pipe 1
2′ of the casing upper part 12 of the small filter 10,
It consists of a one-piece plastic molding, the side of which facing the filter membrane 10' has a number of webs 28, which form a specific filter support and define a number of free spaces 29. This free space is connected to the inlet/outlet tube 12'. The filter support member 28, which supports the flat filter engraving 10', is curved relative to the generally flat surface and has a casing lower part 9/'.
concavely curved at the closed surface of the filter support member 31 below i6 and thus the two casing parts 12
．． A flat filter engraving sandwiched between surrounding flanges 34, 34' of 9 or 6] 0' is axially movable between two filter support members 28, 31. The flat filter engraving 10' is arranged between two filter support members 28, 31 depending on the varying pressure ratio.
Seed pumping movements can also be performed. The two housing parts 12.9 or 12.6 are connected to each other in a leaktight manner by gluing or ultrasonic welding at the raised collar 35' or 350 circumferential surface.

The casing lower part 9 according to FIG. 3 or the casing lid 6 of the canister-type filter device according to FIG. Depending on the viscosity of liquid F in reservoir 4,
By shaking the liquid F, droplets are formed on the casing lid 6.
or into the tube 9', only relatively small droplets F/ will pass through this small hole 2, as shown in FIG.
7" or 27'. If the liquid F is based on an injected aqueous solution, the diameter of this small hole 27' or 27" is particularly small relative to the total length of the two holes. It's 2 fights.

The specific filter support element 31 for flat filter engravings, which has a relatively low specific inherent strength, forms a flat surface and exclusively covers at least one area within the periphery of the flat filter engraving 10'. It is interrupted by two annular passages 33 and at least one blocking passage 32, which intersect the annular passage 33 and the small hole 2 with the collecting passage.
7' or 27'. As can be seen in FIG. 5, the filter support member 31 is interrupted by four blocking passages 32 which divide the filter support member 31 into four annular segments like chronolobes. ing.

The collection channels 27', 27'' actually end at the blocking channel 320 inlet 300 channel bottom, which is 1 m wide at the inlet 30.
Starting from m, it expands towards the annular channel 33 from 3 to 5111I++, depending on the filter diameter.

If the total diameter of the filter is 25111 m, this channel bottom should start from 0.5 mm and point towards the annular channel 33.
Tilts downward towards me. Again, the grammeter was for the viscosity of the aqueous solution.

The bottom of the blocking channel is approximately 0.3 to 0.8 m11°, in particular 0.5 mm, below the surface of the filter support in the area of the collecting channels 27', 27'', slopes downwards, and is inclined downwardly, and extends below the surface of the filter support member.
It is dug down in the order of 1.1 to 16, especially 1.3. For a total diameter of 25 m, this annular passage is at least 2 mm wide and 1.3 mm deep. The inlet 30 opening into the collection passages 27', 27'' of the blocking passage is formed narrower than the opening of the collection passage 271°27'' within this range, and the diameter of the droplet F' from the liquid reservoir ( (depending on viscosity). The apex of the concavely curved filter support member 28 of the casing upper part 12 is connected to the flat filter engraving 10 supported on the lower filter support member 31.
about 0.5~ above this flat filter engraving for '
2 mrtr and in the edge area the upper filter support member 28 is located above the plane of the flat filter engraving 10/0
．． 1 mm to 0.2 v+m.

The filter membrane 10' is 1
Preferably, it has a diameter of 0 to 25 I+IWL.

According to FIGS. 3 and 4, a flat filter engraving 10
' is shown adjacent to the upper filter support member.

According to FIGS. 1 and 4, this means that the connecting member 9'
Or this may occur if the gas pressure in the gas-liquid reservoir 4 is greater than the gas pressure in the connecting tube 12' or the ambient atmosphere. In such a situation, an outlet pipe may be provided in the large filter casing 5, 14 according to FIGS. This occurs when 12' is loaded with pressure when closed.

In contrast, a flat filter engraving 10' directs the liquid F to the casing bottom 14, when the coupling member 12' is opened.
15.18 contacts the lower flat filter support member 31 when suctioning through the filter membrane 19 by means of a vacuum source connected in 18.

In fact, one or more droplets F' are deposited in the free space 32.
33, the droplet F' enters the blocking passageway entrance 3.
0, the entire surface of the flat filter engraving 10' is moistened by the chopped droplets p/ or remains moist in places without the interruption passage 32 and the annular passage 33
distributed inside. That is, the areas of the filter engraving 10' which are always flat are free spaces 27', 27", 32 and 3
3, but also within the scope of the unique flat filter support member 31, which does not contain liquid, so that the tasks provided by the hydrophobic filter membrane 10' are guaranteed even under extreme conditions.

flat filter engraving, with relatively high inherent strength;
For example, for those reinforced with fleece or fabric, the flat filter engraving 10' according to FIG. 4 is supported by the upper casing 12 of the small filter 10 only upwardly.

That is, it is supported against pressure from the upper part of the casing 5 when the filter device is loaded with pressure by the inlet pipes 7, 71. The small holes 27' according to FIG. 4 are enlarged to the edge (27/// shown closed) of the annular passage 33, so that the free filter surface is completely exposed to gas exchange. Ru. Continued wetting of the hydrophobic filter element 10' thereby does not occur. Coupling member 12
The loading of the filter element 10' caused by the necessary ventilation through the filter element 10' remains low when the filter device is operated in suction operation (via the outlet 16) or vented for aerobic eggs. The flat filter engraving 10' preferably has a diameter between 10 and 25 mm when selecting the filling capacity of the filter canister.

In the special case of sterility testing, all filtration devices shown in FIGS. 1 to 4 are sterilized, closed, and aseptically packaged.

According to FIG. 6, the three filtration devices according to FIG. 1 are combined into a functional unit via a common puncture cannula 21 via a filling tube 11, a branch part 24 and a filling tube section 11/. and held in magazines 25,26. The magazine 25, 26 consists of a dish-shaped lower part 25 adapted to one of the contours of the filtration device and a cover 26 connected to the flange part of the lower part 25, which cover is likewise suitable for sterilized devices and device parts. Forms a sterile, sealed encapsulation.

FIG. 7 shows, as in FIGS. 1 and 6, a small filter integrated in the housing lid 6 in the embodiment according to FIG. 4 as a unit of use. With the exception of the coupling adapter 23, it corresponds to the tube and magazine shown in FIG. For kits consisting of two filter canisters with a correspondingly reduced coupling tube 11.24, the magazine 25.26 has a reduced width.

[Brief explanation of the drawing]

FIG. 1 is a vertical cross-sectional view of the sealed and sterile filtration device; FIG. 2 is a detailed cross-sectional view showing the joining points of the filtration canister top and filtration canister bottom as well as the modified tiers; FIG. 3 is a vertical cross-sectional view of a small filter for gas filtration, taken along line 3/4-3/4 in FIG. 5, and FIG. 4 is a vertical sectional view along line 3/4 in FIG. - Vertical sectional view showing a small filter integrated into a large filter casing for the intake and exhaust of a filter canister closed by 3/4, FIG. 6 and 7 each show one unit held in one magazine; FIG. FIG. 3 is a plan view showing a variant of the kit consisting of three filter devices connected to the . 1, 2, 3.4... truncated cone step, 5... transparent filtration canister - upper part, 5'... lower edge, 5", 14'...
Edge, 6.9...lower casing, 7.7/...inlet pipe, 8゜12'...connecting pipe, 10...small filter, 10'...flat filter engraving , 11... Filling pipe, 12... Casing upper part, 13... Removable stopper, 14... Filtration canister - lower part, 15...
Casing chamber, 16... Outlet pipe, 18... Permeable filter support member, 19... Hydrophilic filter membrane, l 9'.
...Hydrophobic edge, 21...Puncture cannula, 22...
・Removable protection tube, 23...coupling adapter,
24...Flow distributor, 25.26...Magazine, 2
7', 27"... Collection passage, 2-17//... Large opening, 28.31... Filter support member, 29...
・Free space, 30... Entrance, 32... Blocking passage, 3
3... Annular passage, 50... Thick part, Ml. M2. M3...step layer, F/...droplet 5n 1n stone i1 Continuation of page 1 @l! Intermediate Butlev Tolmann Federal Republic of Germany Boffenden Drestnerstrasse 1 @l! - Intermediate Horst Berl, Federal Republic of Germany, Göttingen-Urmann-Etzgersu Straße 2 @Inventor, Matsusout Kalvachsch, Federal Republic of Germany, Göttingen-Gehrenring 11

Claims (1)

Claims: 1. A filtration canister with a transparent filtration canister-shaped casing top and an inlet pipe and a filling height mark formed in a number of chamber walls for the measured filling volume and the filtration medium. In a filtration device made of plastic, the upper part has an outlet and the lower part of the casing is connected in a leak-proof manner, and the transparent filtration canister is divided into two chambers. The steps of the platform form a surrounding (filling height gauge (Ml to M3)), in which case the lower first truncated conical step (1) facing the wide base is the filter surface of the filter (19). Approximately 50m from to the 10th layer (Ml)
1, the second and first frustoconical stages (1 and 2) together have a filling capacity of approximately 75 m1, and the third. the second and first frustoconical stages (1 and 2 and 3) together have a filling capacity of about 100 m1 or optionally about 119 m; the last frustoconical stage up to the casing lid (6); (4) A filtration device made of plastic, characterized in that the filling capacity is about 10 to 20 ml. 2 Inlet pipe (7°7/
) is arranged directly on the inner casing wall of the uppermost frustoconical step (4) and to an extent of approximately up to 5 ml above the highest filling height mark (M3) in this uppermost frustoconical step. The filtration device according to claim 1, which extends into the casing. 3. Fewer tiers (both chamfered inside the filtration canister top (5) and adjacent truncated conical tiers (1, 2, 3,
The filtration device according to claim 1 or 2, which has shifted to aspect 4). 4. Patent claim in which the steps differ in diameter from one frustoconical step (1 to 4) to the next frustoconical step (1 to 4) by an amount of 1 to 2 times the casing wall thickness. The filtration device according to any one of the ranges 1 to 3. 5. One truncated conical stage (1 to 4) serves as a casing reinforcing member capable of applying positive and negative pressure over the entire length of the casing, as if fitted with a corset. 4) A filtration device according to claim 1, wherein the filtration device is formed by a thickened portion (50) of the casing wall at the transition point from 1 to 4). 6 The filter diameter of the filter (19) in the lower casing N (i4) is between 40 and 60 mm and the wall slope of the filter canister top (5) is between 05 and 2 mm relative to the vertical axis.
A filtration device according to any one of claims 1 to 5, running at an angle of .degree. 7. In a filtration device consisting of plastic for sterile filtration, sterility testing and storage of liquid samples, a) a transparent casing upper part in the form of a filtration canister (5);
has the shape of a 4-step hollow truncated cone, and the steps of this hollow truncated cone form a surrounding filling height mark (Ml-M3), in which case the lower first one facing the wide base The frustoconical stage (1) has a filling capacity of about 5 Qml from the filter surface of the lower filter (19) to the tenth stage layer (Ml), and the second and first frustoconical stages (l and 2) Approximately 75m long
It has a filling capacity of 1 and 3. Second and first truncated conical stages (
1 and 2 and 3) together have a filling capacity of about IQQml or optionally about 110ml and the casing lid (
The filling capacity of the last truncated conical stage (4) up to 6) is approximately 10~
20 ml, and b) in the casing lid (6) the filter canister top (5) protrudes into the casing interior to a filling height of approximately 5 ml above the highest filling height elevation (M3). Hose connection for a filling hose (11), which has seven inlet pipes, this first inlet pipe being able to be rigidly connected to the hose connection pipe (7) on the outside of the filtration canister top (5). C) a permeable filter support member (C) having a closable outlet pipe (16) and forming a small casing chamber (15);
18) - lower part (14) with 0 pore size
supporting a hydrophilic filter membrane (19) having a diameter of 2μ to 045μ;
The hydrophobic edges (19) surrounded by this hydrophilic filter membrane include the lower edges (5) of the filtration canister upper part (5) and the upper edge (14') of the filtration canister lower part (14). This hydrophobic edge between the upper casing part (5') and the upper casing part (14) as a selectively permeable separating wall is sandwiched in a leaktight manner between the surrounding flange part (5') and the web part (14). 5) from the lower casing part (14),
One of the two edges (5") of the two casing parts (5,1/I) overlies the outer edge (14') of the other casing part (14) in the axial direction and In some cases, the sandwiched water-proof edges (19Q) of the lower filter (19) may be additionally fitted to the casing (5) so that they are airtight and leak-tight.
, 14), in this case the axially and possibly radially overlapping edges (5) of one casing part (5) in such a way that they are shielded from the surrounding atmosphere. 14) and d) a large opening integrated into the casing lid (6) with a small filter (10) arranged in the casing lid (6) as an intake-exhaust filter; part (27), a flat hydrophobic filter engraving (consisting of 10 pieces) with a hole diameter of 02μ to 045μ that covers this opening, and this flat hydrophobic filter engraving covers the filter support member (28) on one side on the edge side.
, the edge of the lid opening between the resting casing upper part (12) with the free space (29) and the connecting pipe (I 2') and the casing lid (6), which is formed as a casing lower part on the other side. In this case, 2
two casing parts (12°6) are joined in a permanently leaktight manner at the edge, the unobstructed filtration area of the flat filter engraving (1o') approximately corresponds to the area of the lid opening, e) the filtration canister; - Filling hose (11) in the upper part (5)
ends upstream of the puncture cannula (21) or coupling adapter (23) with a removable protective tube (22) on its side and optionally downstream downstream of the puncture cannula (21) or coupling adapter (23). Combined in a sterile unit of use, closed in the form of a double or multifold kit with the characteristics described above together with other sterile filtration devices via an arranged flow distributor (branching part) (24) A filtration device made of plastic, characterized in that it is held in a sterile and sealed magazine (25, 26). 8. In filtration devices made of plastic for sterile filtration, sterility testing and storage of liquid samples, a) a transparent casing upper part in the form of a canister (
5) has the shape of a four-stage hollow truncated cone, and the stepped portions of this hollow truncated cone form an surrounding (filling height gauge line (M1 to M3));
In this case, the lower first truncated conical step (1
) has a filling volume of approximately 59 m1 from the filter surface of the lower filter (19) to the first stage layer (Ml), and the second and first truncated conical stages (1 and 2) - together Approximately 7
It has a filling capacity of 5ml and the 3rd. the second and first frustoconical stages (1 and 2 and 3) together have a filling capacity of approximately 100 ml or optionally approximately 110 mJ; the last frustoconical stage up to the casing lid (6); The filling capacity of (4) is about 10-2Qml, and b) the casing lid (6
), the filtration canister top (5) is inside the casing with a maximum of 5 ml above the highest filling height mark (M3).
a first inlet pipe (having seven,
This first inlet pipe can be rigidly connected to a hose connection pipe (7) on the outside of the filtration canister top (5) with a filling hose (
11) forming a hose connection pipe (7) for C) a second connection pipe (7) arranged in the casing lid (6);
8) is the outside of the filtration canister top (5) with a pore size of 02μ.
A reel (for a small filter (10) projecting outwardly from the casing lid (6) with a filter surface (10') supported on both sides of the casing lid (6) as an intake-exhaust filter with a diameter of ~045μ. Luer) type cone and/
or has a thread, and this small filter connects to the second connecting pipe (8
) connected in a leak-tight manner, but removable if necessary, and equipped with a removable stopper (13) at the other connection (12) of this small filter; d) a permeable filter support member (15) having a closable outlet pipe (16) and forming a small casing lid (15);
18) - lower part (14) with 0 pore size
The lower edge (5) of the filtration canister top (5) supports a hydrophilic filter membrane (19) having a diameter of between 2μ and 0.45μ and has a surrounding hydrophobic edge (19'). ') and the upper edge (14') of the filtration canister lower part (14), which is sandwiched between the surrounding flange parts (5) and the web part (141) to provide a leak-tight seal. The upper casing part (5) is separated from the lower casing part (14) as an interposed and selectively permeable separating wall, in which case both edges ( 5") one of the other casing parts (14
The outer edges (14) of the lower filter (19) are covered axially and optionally additionally by the sandwiched water-retardant edges (19) of the lower filter (19) to ensure that the casing (19) is airtight and leak-tight.
5, 14,) so as to be shielded from the surrounding atmosphere, in this case one casing part (5, 14,)
) axially and sometimes radially overlapping edges (
5") are welded or glued with other casing parts (14); e) the filling hose (11) of the filtration canister top (5);
ends upstream of the puncture cannula (21) or coupling adapter (23) with a removable protective tube (22) on its side and optionally downstream downstream of the puncture cannula (21) or coupling adapter (23). Combined in a sterile unit of use, closed in the form of a double or multifold kit with the characteristics described above together with other sterile filtration devices via an arranged flow distributor (branching part) (24) A filtration device made of plastic, characterized in that it is held in a sterile and sealed magazine (25, 26). 9 In the case of a filtration device with a small filter (10) as an intake-exhaust filter, the annular passage (33) has at least two to
9. Filtration device according to claim 8, having a width of 4 mm. A blocking passageway (32) capable of handling 102 forward reactions connects the filter support member (31) of the lower part of the casing (6, 9) with 2
A filtration device according to claim 8 or 9, which is divided into two fan-shaped or annular sections. 11 The filter support member (31) of the lower part of the casing (6, 9) is connected to the collecting passage (27) centrally arranged like a leaf by three or four blocking passages (32) K.
12. Filter in the upper part of the casing (12), which is divided into four annular portions having a diameter of 12 cm and 27 cm. The support surface of the support member (28) is curved concavely relative to the flat filter support member (31) of the casing lower part (6, 9), and this support surface rests at its apex on the lower filter support member (31). The flat filter engraving (approximately 0.5 to 2 mm above the flat filter engraving and supporting member (
28) above or in the plane of the flat filter engraving within the edge area of claims 8 to 11.
The filtration device according to any one of the preceding paragraphs. 13 Collection passage (27', 27" of blocking passage (32)
) an inlet (30) opening into the collection passage (
27', 27") and narrower than the diameter (depending on viscosity) of the droplet (F/) from the liquid reservoir (4). 14. A filtration device consisting of plastic for sterile filtration, sterility testing and liquid sample storage, comprising: a) a transparent casing upper part in the form of a filtration canister (5);
has the shape of a four-step hollow truncated cone, and the steps of this hollow truncated cone form surrounding filling height marks (M1 to M3), and in this case, the lower first one facing the wide base The frustoconical stage (1) has a filling capacity of approximately 50 m1 from the filter surface of the lower filter (19) to the first stage layer (Ml), and the second and first frustoconical stages (1 and 2 ) is about 75m long
It has a filling capacity of 1 and 3. Second and first truncated conical stages (
1 and 2 and 3) together have a filling capacity of approximately IQQ++t/4 or optionally approximately 110 m1, and the filling capacity of the last truncated conical stage (4) up to the casing lid (6) is approximately 10 ~20m1, b) casing lid (6
), the filtration canister top (5) is inside the casing with a maximum of 5 ml above the highest filling height mark (M3).
a first inlet pipe (having seven,
This first inlet pipe can be firmly pressure connected to the hose connection pipe (7) on the outside of the filtration canister top (5).
C) a permeable filter support member (11) forming a hose connection pipe (7) for C) having a closable outlet pipe (16) and forming a small casing chamber (15);
18) - lower part (14) with 0 pore size
The lower edge (5) of the filtration canister top (5) supports a hydrophilic filter membrane (19) having a diameter of between 2μ and 0.45μ and has a surrounding hydrophobic edge (19'). This hydrophobic edge between the upper edge (14') of the filtration canister lower part (14) is sandwiched between the flange parts (5) and the web part (14). Separating the upper casing part (5) from the lower casing part (14) as a leak-tight, sandwiched and selectively permeable separating wall, in which case both edges of the two casing parts (5, 14) One of the parts (5") is connected to the other casing part (14)
The casing (5) axially overlies the outer edge (14') of the casing (5) and optionally additionally ensures that the sandwiched water-tight edge (19') of the lower filter (19) is gas-tight and leak-tight. , 14) in such a way as to be shielded from the surrounding atmosphere, in this case one casing part (5
) axially and sometimes radially overlapping edges (
5') are welded or glued to other casing parts (14), and d) free spaces (27', 30
A small filter (10) arranged in the casing lid (6) as an intake-exhaust filter with a filter support (31) integrated in the casing lid (6) with - side pore diameter o2μ~045
supporting a flat hydrophobic filter engraving (10') of μ;
This flat hydrophobic filter engraving covers the filter support member (28), the free space (29) and the connecting tube (
a mounted casing upper part (12) having 12 ribs;
On the other side, the casing lid is formed as the casing lower part (
6), and in this case, 2
The two casing parts (12, 6) are permanently connected at the edge in a leak-tight manner, and the flat filter engraving (1o') is attached to one or the other filter support element (28, 31) depending on the direction of the pressure gradient. dl) The filter support member (31) of the lower casing (6, 9) forms a flat surface and a closed surface, and the free space of the filter support member forms a flat filter engraving (1).
Only one of width at least 2 vnm within 0 circumferences
one annular passage (33) and at least one blocking passage (
32), and this blocking passage is formed by an annular passage (3
3) into the filtration canister.
', 27''), and d2) the blocking passage (32) starts from the collection passage opening and
From the dragon to the annular passage (33), it expands to 3 mm with a filter diameter of up to 25III, and d3) the bottom of the blocking passage is approximately 0.3 to 0.8 below the filter support surface within the range of the collection passage (27'). mm, inclined downward and recessed 1.1 mm to 1.6 mm towards the annular passage (33); (4) directly from the wide base facing the casing lower part (6), sandwiched between the diameters of the two beads with a length of 21 nWt within the range of the filter support member (31) of the casing lower part (6); Filling hose (11)
ends upstream of the puncture cannula (21) or coupling adapter (23) with a removable protective tube (22) on its side and optionally downstream downstream of the puncture cannula (21) or coupling adapter (23). Combined in a sterile unit of use, closed in the form of a double or multifold kit with the characteristics described above together with other sterile filtration devices via an arranged flow distributor (branching part) (24) A filtration device made of plastic, characterized in that it is held in a sterile and sealed magazine (25, 26). 15. In the case of a filtration device with a small filter (1o) as an intake-exhaust filter, the annular passage (33) has at least two
15. The filtration device of claim 14, having a width of ˜4IIIilO. 16 Two diametrically opposed blocking passages (32) connect the filter support member (31) of the casing lower part (6, 9) with two
16. A filtration device according to claim 14 or 15, which is divided into two fan-shaped or annular sections. 17 The filter support member (31) of the lower part of the casing (6, 9) is centrally arranged like a leaf with three or four blocking passages (32) and the collection passage (27)
18. Filter in the upper part of the casing (12), which is divided into three or four annular parts having a diameter of 18. The support surface of the support member (28) is curved concavely relative to the flat filter support member (31) of the casing lower part (6, 9), and this support surface rests at its apex on the lower filter support member (31). approximately 0.5 to 2 mm above this flat filter formation relative to the flat filter formation (10') K and above or in the plane of the flat filter formation in the area of the edge of the support member (28). The filtration device according to any one of claims 14 to 17. 19 Collection passages (27', 27'' of the blocking passage (32))
) an inlet (30) opening into the collection passage (
27', 27") and narrower than the diameter (depending on viscosity) of the droplet (F') from the liquid reservoir (4). Any one of the above (filtering device described in ()).