JPH02231019A - Method for culturing aphanothece sacrum laver - Google Patents

Abstract

PURPOSE:To enable culture of Aphanothece sacrum over a long period by allowing laver bodies of Aphanothece sacrum to float into water having definite temperature and simultaneously continuously exercising the laver bodies and removing minute fragments produced from the laver bodies out of the system. CONSTITUTION:Laver bodies of Aphanothece sacrum is allowed to float into a water kept at 15-23 deg.C and simultaneously 0.2kgf/cm<3> air is fed to the above- mentioned water every 7.5l per minute to continuously exercise the laver bodies. Then a minute fragment produced from the above-mentioned laver bodies is removed out of the system using a filter device, etc. to artificially and readily carry out culture of Aphanothece sacrum. Furthermore, temperature of the above-mentioned water is 20+ or -2 deg.C and quality of Aphanothece sacrum added to the water is about 1wt.% based on weight of culturing liquid in a state sufficiently removed by a naturally dropping method.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for artificially cultivating Porphyra japonica. [Conventional technology] Daffodil seaweed is produced in freshwater and has an appearance similar to normal seaweed (not necessarily paper-like), and has been prized since the Edo period for its delicious taste. This method takes advantage of natural growth in rivers that There are only a limited number of rivers that are used for the cultivation of Porphyra, and cultivation sites are located near springs. Therefore, a shallow water channel was created using a river, and the flow was similar to a babbling stream.The water bubbles formed in the river, and the floating watermelon was caught and collected, resulting in the creation of a watermelon aquaculture farm. It seems that various attempts have been made to artificially cultivate the Porphyra spp. away from rivers, but there is currently no detailed literature or success stories regarding this. As mentioned above, there are only a limited number of rivers that are suitable for growing Porphyra, and production based on natural reproduction in flowing river water is extremely unstable. This is becoming increasingly difficult from both social and environmental perspectives.

[Problem that the invention seeks to solve]

The present invention has been made based on the above-mentioned viewpoints, and its purpose is to circulate water without directly using rivers, and to rapidly grow Daffodils. The purpose of the present invention is to provide a highly productive method for cultivating Porphyra japonica, which can use most of the water. Therefore, in the Aquarium aquaculture farm, algae growth progresses in a state where algae bodies are scattered or localized in the murmuring of shallow water, but from an extension of this mechanism, it is difficult to synthesize the above problems. It is difficult to solve the problem.

As a result of many attempts, the results were as follows: 1) Suspended the sagebrush in the water and kept it moving vigorously in the water. ■ Remove the microcellular fragments generated from Porphyra japonica out of the system. ■ Maintain the temperature of water at around 15-23℃. This was the first time that it was found that the above-mentioned problems could be comprehensively solved, and the present invention was completed. [Means for solving the problem] Therefore, the above purpose is to suspend the algae of Algae algae in water at a temperature of 15 to 23°C, and to continuously move the algae to collect the minute fragments produced from the algae. This is achieved by a cultivation method for Aphragmium japonica, which removes it from the system. [Function] According to the method for cultivating A. japonica according to the present invention, A. japonica can be cultivated artificially and easily by recycling the water without being extremely limited to the type of water used. be. With the discovery of a cultivation method that allows algae bodies to constantly move vigorously and freely in water, the extreme limitations on the types of water used were lifted, and the basis of the present invention was completed. Daffodils are a colony in which many cells are scattered like spilled beans in a common agar substrate, and the cells divide and increase in number, and the colony becomes thick. They reproduce only by division, which is growing thicker. The size of Suizenjinori when harvested is
It is about 20 to 50 meters long and 50 meters wide, and about 1 to 2 meters thick, which is a completely different size from microalgae that are normally cultured. Furthermore, the size of Daffodils at the start of aquaculture is
It measures about 5 to 10 square meters in length and width, and its growth rate is very slow compared to microalgae. Therefore, the concept of the aquaculture method according to the present invention, in which algae are suspended in water and moved vigorously throughout the water, is completely different from conventional aquaculture methods. The algae, that is, the colonies, of Porphyra aeruginosa are soft and easily destroyed mechanically, and the best way to suspend such algae in the water and make the entire water move violently is to use compressed air. Are suitable. The density of algae for which cultivation is proceeding smoothly is approximately 1 to 3% based on the weight of water used, based on algae that have been sufficiently drained using the natural falling water method.
range, preferably in the range of about 1 to 2%, and at the start of cultivation, algae with a density of about 1% are added to the water as seed algae,
A suitable method is to continue culturing until the density is approximately 2%. Taking these conditions into account, it is preferable to have a culture tank with a structure in which light is supplied from above and compressed air is supplied from below as a large surface. Considering the light receiving conditions of the algae bodies, etc. Depth is about l
A suitable distance is within m, preferably about 0.5 m. In addition, as compressed air, for example, ventilation pressure is 0.2 kgf.
/c+i, it is necessary to supply at least 150 to 200 ffi per minute from the water supply. In addition, as a method for supplying compressed air over a wide area, it is appropriate to supply it through a large cylindrical styrene air stone. When aquaculture is carried out using recycled water, microscopic fragments, especially those whose main part is the substrate, are generated and aggregated from the algae, and these serve as food for microorganisms that are parasitic to the algae, promoting their proliferation. , a problem arises in that it damages the algae.

Although this is not a fatal problem in the case of very short-term aquaculture, it becomes a fatal problem in the case of long-term aquaculture, so it is important to promptly remove such fragments from the aquaculture system. is necessary. The method for removing the fragments is to make the bottom of the aquaculture tank a double bottom, form the upper bottom with a mesh of about 2W, for example, Saran net, and pour water containing the fallen fragments through the mesh of the upper bottom. A suitable method is to introduce water out of the system using a pump, remove fragments using a filtration device, and then circulate the water. Also, polyester/6 is used as a filter medium.
Denier etc. are suitable, and it is sufficient that the water can be filtered and circulated 2 to 4 times per hour. The growth rate of microorganisms that are parasitic to A.
The higher the temperature of the water, the greater the increase, and on the other hand, the optimum temperature for the growth of Daffodils is expected to be quite low since the farm is close to spring water. However, since long-term artificial culture using various water temperatures was not possible, the appropriate water temperature for maintaining stable culture over a long period of time was not clear. Therefore, as mentioned above, a large amount of compressed air is supplied to the water, the algae are suspended in the water, and are moved vigorously and freely throughout the water, while minute fragments of the algae are quickly removed from the system. By using an aquaculture method with these characteristics, the appropriate water temperature range became clear for the first time, and the present invention was completed. In other words, when the temperature exceeds 25 degrees Celsius, the growth of Astragalus becomes abnormal, causing peeling of the substrate, and when the temperature drops below 15 degrees Celsius, the growth rate begins to decline steadily.In addition, considering the suppression of the growth of parasitic microorganisms on the temperature of 15
It was found that maintaining the temperature within the range of ~23°C was appropriate. Therefore, in order to maintain the temperature of the water in the range of 15 to 23 degrees Celsius, a cooling device must be installed in the middle of the pump circulation system.
Although it is possible to use a method in which the water is directly cooled using the above-mentioned cooling device or a method in which the cooling water is sent to a heat exchanger provided in the water for cooling, the latter is the more suitable cooling method. Also, as a heat exchanger, vinyl chloride pipes, polyethylene pipes, etc. with a wall thickness of about 2 mm are most suitable. [Examples] The details of the present invention will be explained below based on specific examples. Examples of the culture tank used in the present invention include those shown in FIGS. 1 and 2, but the tank is not limited thereto as long as it can accomplish the method of the present invention. In addition to sunlight, various artificial light sources (fluorescent lamps, high-pressure sodium lamps, BOC lamps, etc.) can also be used as a light source. Note that most of the detailed studies regarding the present invention were conducted using the apparatus shown in Figure 1. FIG. 1 is an explanatory diagram showing one embodiment of a device for carrying out the method for cultivating Porphyra japonica according to the present invention, and FIG. 2 is an explanatory diagram showing another device. In Figures 1 and 2, ■ indicates a culture tank with a double structure;
is a filtration device, 3 is a ventilation pipe, 4 is an air stone, 5 is a water circulation pipe, 6 is a filter for filtration, 7 is a water filter, 8 is a heat exchanger, 9 is an air pump, 10 is a water circulation pump, l1 is a circulation Pump timer, 12 is the aquaculture tank stand, 13 is the cooling part (tala), 14 is the filtration equipment stand, l5
16 is the steel bottom, 16 is the ventilation air, and 17 is the cooling system piping. Therefore, we prepared two devices as shown in Figure 1, and used water from the Koganegawa River in Amagi City, Fukuoka Prefecture, where Suizenjinori is currently being cultivated, for one side, and water from Chigasaki City, Kanagawa Prefecture for the other. Tap water(
Dechlorinated) 40 each! After adjusting the water temperature to approximately 20 ± 2°C, 1% of each water weight was added to each algae based on the amount of algae of 5 to 10 squares, which had been thoroughly drained by the natural falling water method. A pump with a pumping capacity of 111/min was used to circulate the water, and was operated for 15 minutes every hour to filter out the fine fragments generated from the water lily. Filter wool (trade name) is used as the filter material, and a diaphragm blower is used to float and move the algae, and compressed air of 0.2 kgf/cm2 is pumped into the culture tank 1 at a rate of 7 minutes per minute.
．． 5l! ．． A spherical ceramic air stone was attached to the air outlet, the water temperature was maintained at 20±2℃, and the water that evaporated during cultivation was replenished with the same type of water, and cultivation was continued for 30 days. ．． After 30 days, the amount of Agarica collected was 1% compared to the dry matter inoculation using Kogane River water.
．． It was 42 times the amount and 1.5 times the amount of Chigasaki City tap water. [Effects of the Invention] According to the method for cultivating A. japonica according to the present invention, it has become possible for the first time to artificially cultivate .A. a. It should be noted that the present invention is not limited to the embodiments described above, but includes all modified embodiments that can be easily conceived by those skilled in the art from the embodiments described above.

[Brief explanation of the drawing]

FIG. 1 is an explanatory diagram showing an embodiment of an apparatus for carrying out the method for cultivating Porphyra japonica according to the present invention, and FIG. １－・・−・−・−・・・１・・−・−・・・・・・・−・
・One aquaculture tank 2・−・・−・・−・・−・・・・・・・−・
・−・−・filtration device 3・1・・・1−−1−
−・・Vent pipe 4−・−・−・−・・・−・1
Air Stone 5・−・・１・・１・・・・・・・・・
−・・−・・−・・Water circulation pipe 6～・・・・・−・
・・・・・１・・・１・・・−・・１ Filter for filtration 7・・・・・・１・・−・−・−・・１・・１・−Suizenjinori 8・１− −1−1111−1・1・−11−−・・・−・・Heat exchange body 9−・−・1・−・・
・・・１－－－１・・・－１－－・－・Air pump l
O・−・・−・−・・−・・−・１・・・１−−−
・・Water circulation bomb 1l・−・１・−・・−・−・−
・−・−・−・Circulation pump timer 12−・−・−
・−−−−−1−−・・−・・−・・1 Culture tank stand 13
・−・・・−・・−・・・−・・−・１・・・
-・Cooling part (Tala) l4−・−・・〜・−・・１・・
−・−・−・One filtering device mount l5・・−・・−・−・・
-・-・1-1-1・-・Single mesh bottom 16-・1・1・
1・−・・−・・1・・・・1・Ventilation air l7−・1・
−・−・−・−・１・−−１−−・・−・・Explanatory diagram showing cooling system piping and other equipment

Claims (1)

[Scope of Claims] 1) The method is characterized by suspending algae of Algae algae in water at a temperature of 15 to 23°C and continuously moving the algae to remove minute fragments produced from the algae from the system. A method of cultivating sagebrush. 2) The temperature of the water for use is 20±2°C, and the amount of Agarica spp. added to the water for use is approximately 1% of the weight of the culture solution after sufficiently draining by gravity. A method of cultivating sagebrush. 3) Air of 0.2Kgf/cm^2 is supplied to the above water at 7/min.
．． Claim 1: Supplying 5 liters of water for continuous movement
2. The method for cultivating Porphyra japonica as described in Section 1 or 2.