JPH0217113A - Externally applying agent for skin - Google Patents

Abstract

PURPOSE:To provide a skin externally applying agent which comprises a ROKUJO extract and/or an OGON extract for both brought into a liposome state. CONSTITUTION:The objective skin externally applying agent comprises (A) liposomes capsuling a ROKUJO extract obtained by extracting the non-cornified young horn of Manchuria red deer or Manchuria deer with ethanol or a mixture of the ethanol and water and/or (B) liposomes capsuling an OGON extract obtained by extracting the root of a Labiatae plant Scutellaria baicalensis with ethanol or a mixture of the ethanol and water. The component A accelerates the cornification rate of a slowly cornified skin and the component B retards the cornification rate of a rapidly cornified skin, thereby normalizing the conification rate of the skin (approaches the turn-over days, 14 days, of a normal corneum layer).

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a novel external preparation for skin. More specifically, the present invention relates to a cosmetic material that normalizes the keratinization of the skin epidermis by containing Rokujiyou extract and Scutellariae extract and Scutellariae extract as active ingredients in the form of ribosomes.

[Conventional technology]

There are cases where Rokujiyo extract and Scutellaria scutellariae extract are individually blended into cosmetics. Rokujiyo extract is known to have a number of effects, including restoring heart function, promoting kidney function, promoting digestive system function, and recovering from muscle fatigue.However, its effect on keratinization of the skin epidermis has not been studied. No examples are seen.

Scutellaria scutellariae extract is known to have anti-inflammatory and antipyretic effects, but no studies have been conducted on its effects on the keratinization of the skin epidermis.

Furthermore, ribosomes containing Rokujiyou extract and ribosomes containing Scutellaria scutellariae extract are blended into cosmetics,
Until now, nothing has been found that normalizes the keratinization of the skin epidermis.

[Problem that the invention seeks to solve]

Most of the cells that make up the epidermis of the skin are called keratinocytes.These cells are born by dividing in the basal layer, the lowest layer of the epidermis, and gradually become flattened and form fine layers and granules. It takes 14 days to reach the stratum corneum and become corneocytes (Jl cell turnover), and these cells take another 14 days to be pushed upwards and finally become corneum. Fall off from the skin surface.

This replacement of cells in the stratum corneum is called stratum corneum turnover, and the number of days required for this is called stratum corneum turnover days.It is also common for keratinocytes to change shape while moving from layer to layer. The nucleus disappears in the stratum corneum. This comprehensive differentiation phenomenon of keratinocytes is called keratinization, and the replacement of epidermal cells due to this keratinization is called epidermal turnover. In normal cases, cell layer turnover and stratum corneum turnover occur. Since the stratum corneum is a part of the epidermis, the turnover of the epidermis is naturally closely related to the turnover of the stratum corneum. Therefore, by measuring the number of days for turnover of the stratum corneum, the rate of keratinization of the epidermis can be seen. The faster the rate of keratinization of the epidermis, the shorter the number of days for turnover of the stratum corneum.

There are two types of abnormal keratosis of the skin epidermis.

One is that it occurs with aging, as the rate of cell division slows down, which slows down the rate of cornification of the epidermis (the turnover of the stratum corneum increases), which causes the cell layer of the epidermis to become thinner, and vice versa. The stratum corneum becomes thicker. As a result, the amount of moisture on the skin surface decreases, resulting in a loss of elasticity and softness of the skin. In order to keep aging skin fresh, people apply lotions and creams to keep the skin moist, but this does not provide a fundamental solution.

Another type of keratinization abnormality is that due to the effects of external ultraviolet rays, dryness, and temperature changes, changes occur in the epidermal cells of the cheeks and forehead, which prevents keratinization from occurring normally, and conversely, the rate of keratinization becomes abnormal. (The turnover period of the stratum corneum is shortened.) As a result, 1 epidermal cells are pushed up into the stratum corneum before keratinization is completed, and 1 the keratinocytes, which should originally be anucleated, have nuclei. A phenomenon occurs in which . When normal keratinization does not occur, sufficient NMF and ceramide are not produced, and the skin's ability to retain moisture decreases, resulting in a decrease in the amount of moisture on the skin surface.

Until now, no cosmetics have been found that normalize such abnormally rapid or slow keratinization.

[Means to solve the problem]

The present inventors found that when a topical skin preparation containing ribosomes containing CR Kujiyou extract was used on skin with a slow rate of epidermal keratinization (longer than 14 days, which is the normal turnover period of the stratum corneum), the rate of keratinization increased. It was found that the turnover rate became faster and normalized (approximately 14 days, which is the normal number of days for turnover of the stratum corneum). In addition, if a topical skin preparation containing ribosomes containing Scutellaria scutellariae extract is used on skin where the rate of epidermal keratinization is fast (shorter than the normal stratum corneum turnover period of 14 days, and nuclei remain in the keratinocytes), keratinization occurs. It was found that the rate slowed down and normalized (the normal turnover period of the stratum corneum approached 14 days, and the nuclei of the corneocytes disappeared).

Furthermore, when these ribosomes are combined in a skin preparation, it has been found that areas of the epidermis where the keratinization rate is slow is accelerated, and areas where the keratinization rate is fast is slowed down and normalized, leading to the completion of the present invention. .

The extract used in the present invention is obtained by extracting the unhorned cut horns (deer mushrooms) of Manshu red deer (Fool) or Manshu deer (Plum blossom 1!) with ethanol or a mixture of ethanol and water. It is also possible to extract using a water-soluble solvent other than ethanol, such as 1,3-butylene glycol or propylene glycol.

The Scutellariae extract used in the present invention is obtained by extracting the roots (Scutellariae) of the Lamiaceae plant with ethanol or a mixture of ethanol and water. It is also possible to extract using a water-soluble solvent other than ethanol, such as 1,3-butylene glycol or propylene glycol.

The ribosome of the present invention can be obtained by applying ultrasound to the three components of Rokujijo extract or Scutellaria scutellariae extract, phospholipid, and water. This ribosome is a spherical endoplasmic reticulum consisting of a single or multilayered bilayer membrane of phospholipids, and the extract of Rokujiyo or Scutellariae extract is taken into the membrane of phospholipids or within the endoplasmic reticulum (encapsulation). .

Cholesterol for the purpose of stabilizing ribosomes.

Glucose, amino acids, higher alcohols, nonionic surfactants, ionic surfactants, etc. can be added. In addition to these methods, ribosomes can be produced by a homomixer method, a thin film method, an injection method, a surfactant removal method, etc., and the ribosomes of the present invention can be prepared by any of these methods.

The phospholipids used for ribosome formation are soybean phospholipids, egg yolk phospholipids, hydrogenated soybean phospholipids, hydrogenated egg yolk phospholipids, synthetic phospholipids, etc., and they can be used alone or in combination of two or more.

In the present invention, the amount of Rokujiyo extract used in the ribosomes blended into the skin external preparation is 0.0001 to 0.2% by weight as a dry product of Rokujiyo extract. 0.0OO
1F! If the amount is less than ffi%, no effect will be observed, and if the amount is more than 0.21% by weight, no significant increase in effect will be observed and it is not economical.

In the present invention, the Scutellariae extract used in the ribosomes blended into the skin external preparation is 0.0001 to 0.1% by weight as a dry product of the Scutellariae extract. o, oooi
No effect is observed when the amount is less than 0.2% by weight.
If the amount is greater than % by weight, no significant increase in effectiveness is observed and it is not economical.

Even when combining two types of ribosomes, one containing Rokujiyo extract and the other containing Scutellaria scutellariae extract, into a skin preparation, the Rokujiyo extract used for the ribosome is 0.000% as a dry product of Rokujiyo extract.
1 to 0.2% by weight, and the Scutellariae extract used for ribosomes contains 0.00% as a dry product of Scutellaria extract.
01 to 0°11i amount%.

When Rokujiyou extract and Scutellaria scutellariae extract are directly blended into cosmetics without converting them into ribosomes, almost no effect is observed.This is because the active ingredients of the extracts do not exhibit their effects until they reach the basal cells. This is thought to be because transdermal absorption is significantly enhanced by ribosome formation. Furthermore, the effect can also be obtained by mixing the extract of Scutellariae and the extract of Scutellariae and then making the ribosomes into ribosomes and adding them to an external skin preparation.

〔Example〕

Next, the present invention will be further explained with reference to Examples, but the present invention is not limited thereto. The numbers in the formulations indicate weight %.

Example-1 Cream 1 ■ Squalane ■ Cetyl alcohol ■ Polyoxyethylene (20) Sorbitan monostearate ■ Polyoxyethylene (20) 10,0 2.0 1.5 2.0 Cetyl ether ■ Vaseline 5.0 ■
Octyldodecyl myristate 10. O ■ Purified water 49. 3 ■ 1,3-butylene glycol 10.0 ■ Methyl paraoxybenzoate 0.2 [Phase] Hydrogenated soybean phospholipid
0.20 Rokujiyo extract A
2,0 @purified water
7.80 Kujiyo extract A is obtained by immersing 100 g of finely chopped Aka deer mushroom in 900 g of a 70% weight ethanol aqueous solution and leaving it for 5 days to obtain a filtered extract. The dry matter of Rokujiyo extract A is 1.7% by weight. Ribosomes are prepared by sonicating the components [phase]~@ at 75°C and cooling to 30°C.

After heating and dissolving components 1 to 2 at 80°C, components 1 to 2 previously heated and dissolved at 80°C were added and emulsified.

Cool to 30°C and add 0.034% by weight of ribosomes prepared with ingredients [phase] ~ @ as dry matter of Rokujiyo extract.
) and stir to mix to obtain Cream 1.

Example-2 Cream 2 ■ Squalane 10.0 ■ Cetyl alcohol 2.00 Polyoxyethylene (20) 1.5 Sorbitan monostearate ■ Polyoxyethylene (20) 2.0 Cetyl ether ■ Vaseline 5.0 ■ Myristic acid Octyldodecyl 10.00 Purified water
49.3 ■ 1,3-butylene glycol 10.0 ■ Methyl paraoxybenzoate 0. 2 [Phase] Hydrogenated soybean phospholipid
0.20 Scutellariae extract A
2. 0@purified water
7.8 Scutellariae extract A is obtained by adding 600 g of purified water to 100 g of Scutellariae and heating to 60°C, then adding I kg of ethanol, leaving to stand for 3 days, and filtering to obtain an extract. The dry matter of Scutellariae extract A is 2.1% by weight. Ribosomes are produced by ultrasonicating the components [phase]~@ at 75℃ and then at 30℃.
Cool until ready.

After heating and dissolving components ① to ① at 80℃, add components ③ to ① previously heated to 80℃ to emulsify, cool to 30℃, and dissolve the ribosome prepared with component [phase] ~ Cream 2 is obtained by adding 0.042% by weight (as dry matter) and stirring and mixing.

Example-3 Emulsion l ■ Squalane ■ Cetyl alcohol ■ Polyoxyethylene (20) Sorbitan monostearate ■ Glyceryl monostearate ■ Stearic acid ■ Cetyl isooctanoate ■ Purified water 5.0 1.0 2.5 ■ Propylene Glycol 8.0 ■ Methyl paraoxybenzoate 0. 2 [Phase] Egg yolk phospholipid 1600 Scutellariae extract B 5.0 @ Purified water
5.0 Scutellariae extract B is obtained by adding I kg of ethanol to 100 g of Scutellariae, leaving it for 7 days, and then filtering it. The dry matter of Scutellariae extract B is 2,
The amount is 311%. Ribosome is a component [phase] ~ @ 50
Prepare by cooling to 30°C using a homomixer.

After heating and dissolving components ■ to ■ at 80°C, emulsifying them by adding components (0.115% by weight as dry matter) is added and mixed by stirring to obtain Emulsion 1.

Example-4 Lotion 1 ■ Ethanol 10,0 ■ 1.3-butylene glycol 2.0 ■ Glycerin 3.0 ■ Methyl paraoxybenzoate 0.2 ■ Purified water
74.8■Synthetic phospholipids
0. 5 ■ Cholesterol
0.1■ Rokujiyo extract B
O85 ■ Purified water
8.90 Kujiyo Extract B is 50%1 of 100g of finely chopped Manshuushika deer mushrooms! The sample was immersed in 900 g of a quantified ethanol aqueous solution and left to stand for 5 days to obtain a filtered extract.

The dry matter of Rokujiyo extract B is 1.6% by weight. Ribosomes are prepared by ultrasonicating components ■～■ at 55°C and then at 30°C.
Cool until ready.

After stirring and dissolving Ingredients ■ to ■, add Ingredients ■ and ribosomes prepared with Ingredients ■ to ■ (0.008% by weight as dry matter of Rokujiyo extract) and mix with stirring to obtain Lotion 1. It will be done.

Example-5 Cream 3 ■Squalane.

■Cetyl alcohol ■Polyoxyethylene (20) Sorbitan monostearate ■Polyoxyethylene (20) Cetyl ether ■Vaseline ■Octyldodecyl myristate ■Purified water ■1,3-butylene glycol ■Methyl paraoxybenzoate [phase] Hydrogenation Soybean phospholipids ■ Rokujiyo extract A @ Purified water [phase] Hydrogenated soybean phospholipids ■ Scutellaria extract A ■ Purified water 10,0 2.0 1.5 2,0 After heating and dissolving ingredients ■ to ■ at 80℃ , add ingredients ■~■ previously heated and dissolved at 80°C, emulsify them, cool to 30°C, and prepare ribosomes containing Rokujiyo extract prepared with ingredients [phase]~@ and Scutellaria scutellariae extract prepared with ingredients @~■. Cream 3 is obtained by adding the ribosomes and stirring to mix.

Example-6 Emulsion 2 ■ Squalane ■ Cetyl alcohol ■ Polyoxyethylene (20) Sorbitan monostearate ■ Glyceryl monostearate ■ Stearic acid ■ Cetyl isooctanoate ■ Purified water ■ Propylene glycol ■ Methyl paraoxybenzoate [phase] Egg yolk phospholipid ■ Scutellaria extract B @ purified water 5.0 1.0 2.5 ■ Soybean phospholipid 0.5 [phase]
Rokujiyo extract A 0. 1■
Polyoxyethylene (60) 0.2 Hydrogenated castor oil [phase] Purified water 9.2 Ribosomes containing Scutellaria scutellariae extract have components [phase] ~@50
At ℃, the ribosomes containing Rokujiyo extract have the following components:
Phase] is heated to 60°C in a homomixer and then cooled to 30°C.

After heating and dissolving components ■ to ■ at 80℃, adding components ■ to ■ previously heated and dissolving at 80℃ to emulsify, cooling to 30℃, and preparing ribosomes containing Scutellaria scutellariae extract prepared with ingredients [phase] ~ @. (0.115 as dry matter of scutellariae extract
Emulsion 2 is obtained by adding ribosomes (0.00171% by weight as a dry product of Rokujiyo extract) containing the Rokujiyou extract prepared in the components @ to [phase] and stirring and mixing. .

〔Effect of the invention〕

The cosmetic of the present invention is a cosmetic that is excellent in normalizing epidermal keratinization by blending a ribosome-formed Scutellaria chinensis extract and a ribosome-formed Scutellaria scutellariae extract, either alone or in combination.

Next, the results of an epidermal keratinization rate normalization test and a keratinocyte normalization test regarding the effects of the present invention are shown.

(Test for normalization of epidermal keratinization rate) 1) Test age 4 for ribosomal Rokujiyo extract
Example 1 and Example 4, and Comparative Example 1 and Comparative Example 2 below, with 60 female monitors aged 0 years or older with slow epidermal keratinization rate as subjects, 15 people in each group. The cream was used every day for two months.

Two months later, the degree of improvement in the turnover of the stratum corneum was measured using the Danzyl chloride fluorescence method.

(Danzyl chloride fluorescence method) Disperse 5% of the fluorescent reagent danzyl chloride in white petrolatum, and apply it to the skin with a 10 mm diameter finchanno (-).
Apply occlusion for 4 hours and fluorescently dye the stratum corneum.Next, irradiate ultraviolet light with a maximum wavelength of 338 nm, and measure the fluorescence intensity of the stratum corneum that has been fluorescently dyed with danzyl chloride throughout the day. do.

The number of days for the fluorescence intensity of danzyl chloride applied to the skin to decrease to the fluorescence intensity of the unapplied area is defined as the number of days for turnover of the stratum corneum, and the normal number of days is 14 days.

Comparative Example 1 Cream A cream was prepared in the same manner except for the hydrogenated soybean phospholipid of Example 1, and Rokujiyo extract A was blended without converting it into ribosomes.

Comparative Example-2 Cream Example-1 Hydrogenated Soybean Phospholipid and Rokujiyo Extract A
Except for this, the others were prepared in the same manner.

Test results The results of the normalization test on the rate of epidermal keratinization using ribosome-formed Rokujiyou extract are shown in Table 1. As is clear from Table 6, the skin external preparation containing Rokujiyou extract of the present invention has a significant effect on the keratinization of the epidermis. This is a new topical skin preparation with excellent speed normalization.

Changes in turnover of stratum corneum in long-term continuous m test *Turnover days are the average value of 15 subjects, standard deviation 2)
Experimental Example-2 and Example- 3, and the following creams of Comparative Example 3 and Comparative Example 4 were used every day for the second month.

Two months later, the degree of improvement in the turnover of the stratum corneum was measured using the Danzyl chloride fluorescence method.

The skin external preparation is a new skin external preparation that is excellent in normalizing the keratinization rate.

Comparative example of the number of days for turnover of the stratum corneum in a long-term continuous use test - 3 Cream Except for the hydrogenated soybean phospholipid of Example 2, Scutellaria scutellariae extract A was blended without being converted into ribosomes, and the rest were prepared in the same manner.

Comparative Example-4 Cream Except for the hydrogenated soybean phospholipid and Scutellaria scutellariae extract A of Cream Example-2, the rest was lll1! Shita.

Test Results The results of the epidermal keratinization rate normalization test using ribosomal Scutellaria scutellariae extract are shown in Tables 0 and 2. Average value and standard deviation 3) Among women aged 40 years or older with a slow keratinization rate, nucleated cells were observed in the cheek area and showed a fast keratinization rate. The subjects were 60 monitors with different body parts. 15 people per group,
The creams of Examples 5 and 6, Comparative Example 5, and Comparative Example 6 were used daily for two months.

Two months later, the degree of improvement in the turnover of the stratum corneum was measured using the Danzyl chloride fluorescence method.

Test results Table 3 shows the results of a test for normalizing the rate of epidermal keratinization using a combination of ribosomal Scutellaria scutellariae extract and ribosomal Scutellaria scutellariae extract of the present invention. Extract liquid-containing skin preparation for external use is
This is a new topical skin preparation that is excellent in normalizing the keratinization rate by speeding up the keratinization rate in areas with a slow keratinization rate and slowing down the keratinization rate in areas with a fast keratinization rate.

Comparative Example 5 Cream The hydrogenated soybean phospholipids in the cream of Example 5 were removed, and the Scutellaria japonica extract A and the Scutellaria scutellariae extract A were blended as they were.

Comparative Example 6 Cream A cream was prepared in the same manner as in Example 5, except for the hydrogenated soybean phospholipids, Scutellaria chinensis extract A, and Scutellaria scutellariae extract A.

Table, 3 Number of days for turnover of stratum corneum in long-term continuous use test (1) Areas with slow keratinization rate (mouth) (2) Areas with fast keratinization rate (cheeks) (keratinocyte normalization test) Test method Ultraviolet rays, humidity, When the rate of keratinization increases due to external factors such as temperature, nucleated cells that are not normally found in the stratum corneum are observed. Therefore, the degree of improvement in the keratinization rate was measured using these nucleated cells as an index.

The measurement method was to use a slide glass with double-sided adhesive tape affixed in a size of 1 x 1 cm, and press the adhesive side for about 2 seconds on the monitor's face 10 minutes after washing the face.
The first layer of the stratum corneum, which can be collected upon peeling, was sampled. The corneocytes collected on the glass slides were stained with hematoxylin and eosin (H, E staining) according to information.

The presence or absence of nucleated cells that were stained blue by hematoxylin was confirmed using an optical microscope at a magnification of 15x20. In the measurement, the number of particles was counted in five consecutive visual fields in one direction. In addition, nucleated cells.

Since the flakes are usually peeled off in clusters, they were counted and given a score based on the following criteria, and the sum of the products with each number was scored.

Below are the margin keratinocyte evaluation criteria. Clusters of 1041 or more with 5 or less keratinocyte status scores alone are monitored by 10 people per group for 40 women with nucleated cells, according to Example-3 and The emulsions of Example 6, Comparative Example 5, and Comparative Example 6 were used daily for 3 months.

Test Results The test results are shown in Table 4. As is clear from Table 4, the external skin preparations of Example-3 and Example-6 are novel skin preparations that reduce the number of nucleated cells and normalize the keratinization rate (turnover time). .

In addition, the skin external preparations of Examples 2 and 5 were also found to have similarly excellent keratinization rate normalizing effects.

Comparative Example-5 Emulsion Excluding the egg yolk phospholipid of Example-3, Ogre Ng extract A
was prepared without converting it into ribosomes, and the other ingredients were prepared in the same manner.

Comparative Example 6 Emulsion The emulsion was prepared in the same manner as in Example 3 except for the egg yolk phospholipids and Scutellaria extract A.

Margin table below, 3. Change in the number of nucleated cells in long-term continuous use test *The number of nucleated cells (score) is higher than the average value of 10 people and the standard deviation

Claims (3)

[Claims] (1) A skin external preparation that is characterized by containing ribosomes encapsulating Rokujiyou extract and normalizing the keratinization of the skin epidermis. (2) Contains ribosomes containing Scutellaria extract,
External skin preparation characterized by normalizing keratinization of the skin epidermis (3) A topical skin preparation characterized by normalizing the keratinization of the skin epidermis by blending ribosomes encapsulating an extract of Scutellaria vulgare and ribosomes encapsulating an extract of Scutellariae.