JPH02142422A - Method and container for culturing mushroom hypha mass - Google Patents

Abstract

PURPOSE:To provide the subject hypha mass in a high yield without being contaminated with various microorganisms by charging a medium in a double- structured container comprising an inner soft container exhibiting air permeability after subjected to a sterilization treatment and an outer heat-resistant container. CONSTITUTION:For example a mushroom-culturing medium 2 such as saw dust or rice bran is charged in an inner soft container 1 (e.g., polyvinyl chloride container) having plural small holes 4, and the inner container 1 is subsequently placed in an outer soft container 3 (e.g., polypropylene container) having a bottom deeper than the container 1, followed by subjecting to a sterilization process, an inoculation process and a culturing process to provide the objective hypha mass.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for culturing a mushroom mycelial mass for use as a seed or for mushroom generation, and a culture container used therein.

The method for cultivating a mushroom mycelial mass and the culture container according to the present invention are capable of obtaining a mushroom mycelial mass capable of producing seed fungi or mushrooms at a high yield without fear of contamination with various bacteria.

[Conventional technology]

Conventionally, there are various methods for culturing mushroom concave thread masses, and one commonly used method is a cultivation method using a container for culturing mushroom mycelia masses. Containers for culturing mushroom mycelial masses include those that use hard containers such as bottles, and those that use soft containers such as those made of polypropylene.

[Problem to be solved by the invention]

If the mushroom culture medium becomes contaminated with various bacteria, the resulting mushroom mycelial mass cannot be used as a seed fungus, or mushrooms may no longer grow. Therefore, it is common sense in the art to take care to prevent the mushroom culture medium from being contaminated with germs when culturing mushroom mycelial masses. For this reason, measures have been taken to inoculate in a sterile room and to ventilate the mushroom culture through as narrow and long a passage as possible.

However, as long as conventional mushroom mycelium culture containers are used, no matter how careful one is, it has not been possible to prevent bacterial contamination of the mushroom culture medium in some of the many mushroom mycelium culture containers. .

As a result of long-term pursuit of the cause, the inventor of the present invention found that
It was found that this was caused by part of the mushroom culture medium adhering to the mouth of the mushroom mycelium culture container. The mouth of the mushroom mycelium culture container is the closest to the outside world where germs are floating, so germs are most likely to adhere to it. and,
In conventional methods for culturing mushroom mycelial masses, a portion of the mushroom culture medium tends to adhere to the mouth of the mushroom mycelium, causing the proliferation of various bacteria.

Therefore, the following two methods can be considered as countermeasures. The first is to prevent part of the mushroom culture medium from adhering to the mouth of the container for culturing mushroom mycelial masses.

However, the opening of the container for culturing mushroom mycelium is narrow in order to prevent bacterial contamination, so when filling the container with mushroom medium, the mushroom medium may adhere to the inside or outside of the opening. That is inevitable. The second method is to clean the mouth of the mushroom mycelia mass culture container to which the mushroom medium has adhered. However, it is difficult to wash or wipe the mouth of a container for culturing mushroom mycelial masses after filling with a mushroom culture medium with water because it is technically and labor-intensive and cannot be carried out.

[Means to solve problems]

Therefore, the inventor of the present invention conducted various researches on the above-mentioned points, and as a result, completed the present invention which can achieve the intended purpose by making the container have a double structure.

In the method for cultivating a mushroom mycelial mass according to the present invention, a mushroom culture medium 2 is filled in a soft inner container 1 that exhibits air permeability at least after sterilization treatment, and the mushroom culture medium 2 is filled with a mushroom culture medium 2 that has a deeper heat resistance than the inner container 1. It is characterized in that it is placed in a soft outer container 3, and then sterilized, inoculated, and cultured according to conventional methods.

In addition, 11 the container for culturing mushroom mycelial masses according to the present invention is made of a soft inner container that exhibits air permeability at least after sterilization treatment.
The inner container 1 is characterized by comprising a soft outer layer 2 and a third layer having a deeper heat resistance than the inner container 1.

An important point in the present invention is the use of a double container, the inner container 1 and the outer container 3.

The inner container 1 is constructed of a material that exhibits air permeability at least after sterilization treatment. Therefore, it is possible to use an inner container 1 that is provided with small holes 4 from the beginning, or an inner container 1 that is melted and opened by the heat during sterilization.
may be used. Furthermore, when a wrapping cloth is used as the inner container 1, ventilation can be achieved through the folded portion.

In this way, the reason why the inner container 1 must have the property of exhibiting air permeability at least after sterilization is because the inoculated starter must come into contact with and adhere to the mushroom medium, and also because the inoculated starter must contact and adhere to the mushroom medium. This is because air must be sent to the seed culture.

If the inner container 1 is to be melted and opened by the heat during sterilization, the inner container 1 must be made of heat-fusible material such as polyvinyl chloride. On the other hand, inner container 1
If it is not necessary to melt and open the opening by the heat during sterilization, a heat-resistant material such as polypropylene may be used.

The container 3 needs to withstand the heat during sterilization, just like the conventional container for culturing mushroom mycelium.

Therefore, it is made of a material such as polypropylene.

Although the present invention can achieve the intended purpose by adopting the above-described configuration, a more excellent container for culturing mushroom mycelial masses can be obtained by adopting the following additional requirements. For example, if the auxiliary hole 5 is provided on the bending line of the mouth of the outer container 3, the mouth of the outer container 3 can be inoculated without having to face upward (so that the inoculation of the seed bacteria can be carried out, so that the Contamination can be more reliably prevented. Furthermore, by providing the auxiliary holes 5, ventilation to the mushroom culture medium 2 becomes easier.

Examples of the mushroom culture medium 3 packed into the inner container 1 described above include a sawdust+rice bran culture medium, a compost culture medium, and the like.

〔Example〕

Next, the present invention will be explained based on examples.

Example 1 A mushroom culture medium made of sawdust rice bran was prepared in a width of 15 cm.
It was filled into 100 inner containers made of polyvinyl chloride bags with a depth of 5 cm and a height of 1 Q cm. Next, wash the outside of this inner container with water and make it into a width 16cm x depth 6C11.
x It was placed in an outer container made of a polypropylene bag with a height of 30 cm, and the mouth of the outer container was bent at a height of about 15 cm. Next, after sterilization according to a conventional method, a mushroom inoculum was inoculated from the mouth of the outer bag, cultured for 25 days, and mushroom mycelial masses were obtained from each mushroom culture container. The results are shown in Table 1.

Comparative Example 1 On the other hand, for comparison, a mushroom culture medium was directly filled into a polypropylene bag without using an inner container, and the other conditions were the same as in Example 1 to obtain a mushroom mycelium mass. The results are also shown in Table 1.

Table 1 *Harmless contamination: Contamination that does not pose a problem for mushroom growth, but is such that it cannot be used as a starter.

Hazardous contamination: Contamination to a certain extent that does not interfere with the growth of mushrooms.

[Function] [Effect] Since the present invention adopts the above configuration, the following effects can be obtained. That is, in the container for culturing mushroom mycelial masses according to the present invention, the inner container filled with the mushroom culture medium and the outer container functioning as the original mushroom culture container are constructed separately, so that the container can be filled with the mushroom culture medium. The inner container can be cleaned by washing it with water or wiping it before putting it into the outer container. Therefore, the mushroom culture medium does not adhere to the mouth of the outer container.

Therefore, even if germs are attached to the mouth of the outer container, there is less risk of them breeding. Therefore, by using the method for cultivating a mushroom mycelial mass and the container for culturing mushrooms according to the present invention, a mushroom mycelial mass from which seeds or mushrooms can be harvested at a high yield can be obtained.

Therefore, the method for cultivating a mushroom mycelial mass and the culture container according to the present invention are suitable for culturing mycelial masses of C. chinensis, maitake, shiitake, enokitake, and the like.

[Brief explanation of the drawing]

The drawings show an embodiment of the container for culturing mushroom mycelial masses according to the present invention, and FIG.
FIG. 2 shows a cross-sectional view of the outer container with its mouth portion bent.

Claims (5)

[Claims] (1) Fill the mushroom culture medium 2 into the soft inner container 1 that exhibits air permeability at least after sterilization treatment, and fill the inner container 1 with the mushroom culture medium 2.
A method for cultivating a mushroom mycelium mass, which comprises placing it in a deep heat-resistant soft outer container 3, followed by sterilization, inoculation, and cultivation according to conventional methods. (2) A mushroom cultivation container characterized by comprising a soft inner container 1 that exhibits air permeability at least after sterilization treatment, and a heat-resistant flexible outer container 3 that has a deeper bottom than the inner container 1. . (3) The container for culturing mushroom mycelial masses according to claim 2, wherein the inner container 1 has a plurality of small holes 4. (4) The container for culturing a mushroom mycelial mass according to claim 2, wherein the inner container 1 is opened by being melted by heat during sterilization treatment. (5) The method for culturing a mushroom mycelium mass according to claim 2, wherein the inner container 1 is a wrapping cloth.