JPH01124329A - Vessel for culturing mycelial pellet of mushroom - Google Patents
Vessel for culturing mycelial pellet of mushroomInfo
- Publication number
- JPH01124329A JPH01124329A JP62283388A JP28338887A JPH01124329A JP H01124329 A JPH01124329 A JP H01124329A JP 62283388 A JP62283388 A JP 62283388A JP 28338887 A JP28338887 A JP 28338887A JP H01124329 A JPH01124329 A JP H01124329A
- Authority
- JP
- Japan
- Prior art keywords
- mushroom
- container
- vessel
- soft
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 55
- 238000012258 culturing Methods 0.000 title claims description 13
- 239000008188 pellet Substances 0.000 title abstract 2
- 239000001963 growth medium Substances 0.000 claims description 26
- 238000011109 contamination Methods 0.000 abstract description 16
- 238000009423 ventilation Methods 0.000 abstract description 8
- 244000052616 bacterial pathogen Species 0.000 abstract 2
- 238000007789 sealing Methods 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 238000011081 inoculation Methods 0.000 description 6
- 239000002054 inoculum Substances 0.000 description 5
- -1 polypropylene Polymers 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 239000004743 Polypropylene Substances 0.000 description 4
- 229920001155 polypropylene Polymers 0.000 description 4
- 239000007858 starting material Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 240000006499 Flammulina velutipes Species 0.000 description 2
- 235000016640 Flammulina velutipes Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 240000001080 Grifola frondosa Species 0.000 description 1
- 235000007710 Grifola frondosa Nutrition 0.000 description 1
- 240000000599 Lentinula edodes Species 0.000 description 1
- 235000001715 Lentinula edodes Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000005060 rubber Substances 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、キノコ菌糸塊培養用容器に関するものである
。より詳しくは、本発明に係るキノコ菌糸塊培養用容器
は、雑菌汚染の防止が確実で良質のキノコ菌糸塊を経済
的に得るのに適したものである。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a container for culturing mushroom mycelial masses. More specifically, the container for culturing mushroom mycelial mass according to the present invention is suitable for economically obtaining a high-quality mushroom mycelial mass with reliable prevention of bacterial contamination.
本発明に係るキノコ培養用容器は、マンネンタケ。The container for cultivating mushrooms according to the present invention is a container for cultivating mushrooms.
マイタケ、シイタケ、エノキタケ等の菌糸塊を培養する
に適したものである。It is suitable for culturing mycelial masses of maitake, shiitake, enokitake, etc.
（発明の技術的背景と従来の技術〕
一般に、キノコ培地に種菌を接種し、キノコ菌糸を蔓延
させてキノコ菌糸塊を得るに際しては、キノコ培地が雑
菌に汚染されないように多大の注意と費用を払う必要が
ある。(Technical Background of the Invention and Prior Art) Generally, when inoculating a mushroom culture medium with a seed fungus and spreading mushroom mycelium to obtain a mushroom mycelial mass, great care and expense are taken to prevent the mushroom culture medium from being contaminated with various bacteria. need to pay.
滅菌後のキノコ培地が汚染される原因のうち最も大きな
ものは次の二つである。即ち、その一つは、種菌接種時
に行うキノコ菌糸塊培養用容器の開口に伴う雑菌汚染で
ある。他の一つは、種菌の培養期間中の通気に伴う雑菌
汚染である。The following two are the most common causes of contamination of mushroom culture media after sterilization. That is, one of them is bacterial contamination due to opening of the container for culturing the mushroom mycelia mass during seed inoculation. Another problem is bacterial contamination due to aeration during the culture of the seed culture.
従来のキノコ菌糸塊培養用容器としては、壜などの硬質
容器を利用したものと、ビニール袋などの軟質容器を利
用したものとがある。前者の最大の欠点はキノコ培地の
出し入れの不便さであ、す、後者の最大の欠点は取り扱
いの不便さである。もっとも、この問題点は、硬質容器
内に軟質容器を収納させたキノコ菌糸塊培養用容器を考
え出すことによって解決されている。ところが、このキ
ノコ菌糸塊培養用容器においては、次のような問題点が
内在しているのである。つまり、■種菌接種時に行うキ
ノコ菌糸塊培養用容器の開口に伴う雑菌汚染については
考慮されていない、■通気に伴う雑菌汚染を防止するた
めには開口部にフィルターを設ける必要がある。Conventional containers for culturing mushroom mycelium include those that use hard containers such as bottles, and those that use soft containers such as plastic bags. The biggest drawback of the former is the inconvenience of taking in and out the mushroom culture medium, and the biggest drawback of the latter is the inconvenience of handling. However, this problem has been solved by devising a container for culturing mushroom mycelial masses in which a soft container is housed within a hard container. However, this container for culturing mushroom mycelial masses has the following problems. In other words, 1. Bacterial contamination caused by opening the mushroom mycelium culture container during inoculation is not considered. 2. A filter must be provided at the opening to prevent bacterial contamination due to ventilation.
〔問題点を解決する手段］
本発明者は、前記の諸点に鑑み種々研究をした結果、完
全な無菌室で接種作業をする場合は別としてそうでない
場合にキノコ培地に対して垂直方向に種菌を接種する以
上、キノコ菌糸塊培養用容器の開口に伴う雑菌汚染を防
ぐことはできないとの結論に達した。そして、この問題
点を解決するためには、キノコ培地に対して垂直方向に
種菌を接種しないようにし、しかもその通路を狭（すれ
ば前記問題点を解決できるのではないかとの知見を得、
かかる知見に基づき本発明を完成したのである。[Means for solving the problem] As a result of various studies in view of the above-mentioned points, the present inventor has found that, apart from when inoculation is performed in a completely sterile room, in other cases, the inoculation is carried out vertically to the mushroom culture medium. We have come to the conclusion that it is not possible to prevent bacterial contamination due to opening of containers for culturing mushroom mycelial masses. In order to solve this problem, we learned that the above problem could be solved by not inoculating the inoculum vertically into the mushroom culture medium, and by narrowing the passage.
The present invention was completed based on this knowledge.
即ち、本発明に係るキノコ菌糸塊培養用容器は、硬質容
器Ａと、キノコ培地を充填した軟質容器Ｂとからなり：
硬質容器Ａ内に収納した軟質容器Ｂの口部１を絞った状
態で硬質容器Ａの側壁に設けた袋絞口２から外に出した
ことを特徴とするものである。That is, the mushroom mycelium mass culturing container according to the present invention consists of a hard container A and a soft container B filled with a mushroom culture medium:
This is characterized in that a soft container B housed in a hard container A is taken out from a bag opening 2 provided on the side wall of the hard container A with its mouth 1 squeezed.
次に、本発明の構成を詳細に説明する。本発明に係るキ
ノコ菌糸培養用容器は、硬質容器Ａとキノコ培地３を充
填した軟質容器Ｂとからなる。本発明に係るキノコ菌糸
培養用容器は、従来のキノコ菌糸培養用容器と同じよう
に、充填したキノコ培地３を滅菌処理する際の加熱に耐
える必要があるから、硬質容器Ａ及び軟質容器Ｂに耐熱
性が要求されることは云うまでもない。Next, the configuration of the present invention will be explained in detail. The mushroom mycelium culture container according to the present invention consists of a hard container A and a soft container B filled with a mushroom culture medium 3. The mushroom mycelium culture container according to the present invention, like the conventional mushroom mycelium culture container, needs to withstand heating during sterilization of the filled mushroom culture medium 3. Needless to say, heat resistance is required.
硬質容器Ａは、例えばポリプロピレン樹脂などで作られ
る。この硬質容器Ａの側壁には袋絞口２が設けられるが
、この袋絞口２は軟質容器Ｂに充填されたキノコ培地３
の上面より若干上の位置に設けるのが次の理由から好ま
しい。袋絞口２がキノコ培地３の上面より余り上にあり
すぎると、雑菌汚染を防止する効果が減少する。つまり
、袋絞口２が硬質容器Ａの上にあればあるほど、キノコ
培地３に対して種菌が垂直方向に近い状態で接種される
ことになるからである。一方、袋絞口２がキノコ培地３
の上面より余り下にありすぎると、種菌を接種し難く、
またその後の通気性が悪化する。なお、この袋絞口２は
、硬質容器の側壁に独立した孔として設けてもよいし、
また第２図及び第３図に示すように切り込み合＾待った
孔であってもよい。The hard container A is made of, for example, polypropylene resin. A bag opening 2 is provided on the side wall of the hard container A, and the bag opening 2 is connected to the mushroom culture medium 3 filled in the soft container B.
It is preferable to provide it at a position slightly above the upper surface for the following reason. If the bag opening 2 is located too far above the top surface of the mushroom culture medium 3, the effect of preventing bacterial contamination will be reduced. In other words, the higher the bag opening 2 is above the hard container A, the more the inoculum will be inoculated into the mushroom culture medium 3 in a vertical direction. On the other hand, the bag opening 2 is the mushroom medium 3.
If it is too far below the top surface, it will be difficult to inoculate the inoculum,
Further, the air permeability thereafter deteriorates. Note that this bag squeeze opening 2 may be provided as an independent hole in the side wall of the hard container, or
Alternatively, as shown in FIGS. 2 and 3, the holes may be cut together.
軟質容器Ｂは、前記した硬質容器Ａ内に収納されるもの
であり、例えばポリエチレンフィルム、ポリプロピレン
フィルム等によって作られる。この軟質容器Ｂは、袋状
のもののみならず、風呂敷状のものであってもよい。軟
質容器Ｂの中には、キノコ培地３が充填されている。キ
ノコ培地３は軟質容器Ｂ −杯に充填されるのではなく
、２／３〜１／２程度が充填される。このキノコ培地３
が入っていない口部１は、前記した硬質容器Ａの側壁に
設けた袋絞口２から外に出される。The soft container B is housed within the hard container A described above, and is made of, for example, polyethylene film, polypropylene film, or the like. This soft container B is not limited to a bag-like one, but may be a wrapping cloth-like one. The soft container B is filled with mushroom culture medium 3. The mushroom culture medium 3 is not filled into the soft container B-cup, but is filled to about 2/3 to 1/2. This mushroom medium 3
The mouth portion 1 that does not contain the bag is taken out from the bag opening 2 provided on the side wall of the hard container A described above.
前記した軟質容器Ｂ内に詰められるキノコ培地３として
は、オガコ＋ヌカ培地、コンポスト培地などを挙げるこ
とができる。Examples of the mushroom culture medium 3 packed in the above-mentioned soft container B include a sawdust+bran culture medium, a compost culture medium, and the like.
本発明は、以上の構成を採ることによって所期の目的を
達成できるが、次に述べるような付加的要件を採ること
によって、より優れたキノコ菌糸塊培養用容器とするこ
とができる。例えば、キノコ培地３を充填した軟質容器
Ｂの口部１の根本に袋絞環４を設けておけば、硬質容器
Ａ内の軟質容器Ｂの通気路を狭めることができるので、
雑菌汚染の防止効果を高めることができる。このような
袋絞環４の材質は特に限定されず、金属製、プラスチッ
ク調成いはゴム製のものを用いればよい。Although the present invention can achieve the intended purpose by adopting the above-described configuration, a more excellent container for culturing mushroom mycelial masses can be obtained by adopting the following additional requirements. For example, if a bag squeeze ring 4 is provided at the base of the mouth 1 of the soft container B filled with the mushroom culture medium 3, the ventilation path of the soft container B inside the hard container A can be narrowed.
The effect of preventing bacterial contamination can be enhanced. The material of the bag diaphragm ring 4 is not particularly limited, and may be made of metal, plastic, or rubber.
また、硬質容器Ａの側壁の袋絞口２の横或いは下方向に
釣手状に突出した引っ掛は部５を設けておいてもよい。Furthermore, a hook portion 5 may be provided on the side wall of the hard container A, protruding in the shape of a hook laterally or downwardly from the bag squeeze opening 2.
これは、硬質容器Ａ外の軟質容器Ｂの口部１の通気路を
狭めることになり、雑菌汚染の防止効果を高めることが
できる。なお、この引っ掛は部５は硬質容器Ａと一体的
に作ることが可能である。This narrows the ventilation path of the mouth part 1 of the soft container B outside the hard container A, and can enhance the effect of preventing bacterial contamination. Note that this hook portion 5 can be made integrally with the hard container A.
さらに、キノコ培地３上に種菌糸が摂食可能な材質から
なる培地薄層（図示せず。）を覆っておけば、この培地
薄層上に落下した種菌を一気に横方向に繁殖させること
ができる。培地薄層は、例えば祇或いは薄板等によって
構成される。必要によっては、これらに菌糸の好む栄養
分を吸収させたり、塗布したりしておいてもよい。Furthermore, if the mushroom medium 3 is covered with a thin medium layer (not shown) made of a material that the seed hyphae can ingest, the seed fungi that have fallen onto this thin medium layer can be propagated laterally at once. can. The thin culture medium layer is composed of, for example, a plate or a thin plate. If necessary, nutrients preferred by mycelia may be absorbed or coated on them.
次に、本発明を実施例に基づいて説明する。 Next, the present invention will be explained based on examples.
実施例１
オガクズ・コメヌカからなるキノコ培地８００ｃｃヲポ
リプロピレン製の袋（内容積的１２００ｃｃ） ニ詰め
、袋の口部を袋絞環に通し、さらに耐熱性ビーカー状容
器（内容積的１２００ｃｃ）の側壁に設けた袋絞口に通
し、これを常法に従って滅菌処理を施し、冷却を待った
。次いで、横向きにした袋の口部からマンネンタケ及び
エノキタケの種菌を接種し、一定期間培養し、各々の種
菌の蔓延した菌糸塊を各々１００袋づつ得た。Example 1 A mushroom culture medium consisting of sawdust and rice bran containing 800 cc was stuffed into a polypropylene bag (inner volume: 1200 cc), the mouth of the bag was passed through a bag squeeze ring, and then the side wall of a heat-resistant beaker-shaped container (inner volume: 1200 cc) was packed. The bag was passed through a bag opening, sterilized in a conventional manner, and allowed to cool. Next, inoculum of Cinderaceae and Enokitake were inoculated from the mouth of the bag placed horizontally, and cultured for a certain period of time to obtain 100 bags each of mycelia masses in which each inoculum was spread.
一方、比較のため、上記と同一のキノコ培地８００ ｃ
ｃを通常のポリプロピレン製の壜（内容積的１２００ｃ
ｃ）に詰め、フィルター付き通気蓋を施し、常法に従っ
て滅菌、接種、培養の処理を施し、上記と同じように各
々種菌の蔓延した菌糸塊を各々１００袋づつ得た。On the other hand, for comparison, the same mushroom medium 800 c as above
c is a regular polypropylene bottle (inner volume 1200c)
c), covered with a ventilation lid with a filter, and subjected to sterilization, inoculation, and culture according to conventional methods, to obtain 100 bags each of mycelia masses infested with each seed fungus in the same manner as above.
その結果を第１表に併せて示す。The results are also shown in Table 1.
第１表
※無害汚染：キノコの発生には差し支えないが、種菌と
して使用することはできない程度
の汚染をいう。Table 1 *Harmless contamination: Contamination that does not pose a problem for mushroom growth, but is such that it cannot be used as a starter.
有害汚染：キノコの発生に差し支えがある程度の汚染を
いう。Hazardous contamination: Contamination to a certain extent that does not interfere with the growth of mushrooms.
軟質容器の口から挿入された種菌は、キノコ培地３に着
地する前に必ず横方向になって転げたり、移動したりす
るため、種菌に付着している雑菌や空気中に浮遊してい
る雑菌が、栄養分のない軟質容器８面に付着してキノコ
培地３への到達競争に遅れをとることになる。そのため
、雑菌の多くは、このときすでに菌糸状に着地している
種菌に対してなんらの影響を及ぼさなくなる。また、通
気路が狭くしかも曲がっているので、雑菌が軟質容器Ｂ
の開口部１から進入してきても、キノコ培地３に至るま
でに軟質容器Ｂの内面に雑菌が付着して枯死してしまう
ことになる。The starter inserted through the mouth of the soft container always rolls or moves laterally before it lands on the mushroom culture medium 3, so the bacteria attached to the starter and the bacteria floating in the air However, the mushrooms adhere to the surface of the soft container 8, which is devoid of nutrients, and fall behind in the race to reach the mushroom medium 3. Therefore, many of the bacteria no longer have any effect on the seed bacteria that have already landed in the form of mycelia. In addition, since the ventilation path is narrow and curved, bacteria can
Even if the bacteria enter through the opening 1 of the soft container B, the bacteria will adhere to the inner surface of the soft container B and die before reaching the mushroom culture medium 3.
本発明は、以上の如き構成を採るから、次の効果を得る
ことができる。即ち、本発明に係るキノコ菌糸塊培養用
容器は、種菌をキノコ培地に対して垂直方向に落下させ
ないので、接種時の軟質容器の口部の開口に関わらず、
キノコ培地が雑菌汚染を受けることがない。また、軟質
容器の口部の通気路が狭くしかも曲がっているので、フ
ィルターを設けなくとも、種菌の培養期間中の通気に伴
う雑菌汚染の恐れが少ない。Since the present invention adopts the above configuration, the following effects can be obtained. That is, since the mushroom mycelium mass culture container according to the present invention does not allow the seed fungus to fall vertically to the mushroom culture medium, regardless of the opening of the mouth of the soft container during inoculation,
The mushroom culture medium will not be contaminated with bacteria. Furthermore, since the ventilation path at the mouth of the soft container is narrow and curved, there is little risk of bacterial contamination due to ventilation during the culture of the seed culture, even without a filter.
第１図は本発明に係るキノコ菌糸塊培養用容器の図を示
す。
図中 Ａ−硬質容器、Ｂ −−−−一軟質容器、１・−
口部、２−・−袋絞口、３−キノコ培地。FIG. 1 shows a diagram of a container for culturing mushroom mycelial masses according to the present invention. In the figure: A - Hard container, B - - Soft container, 1.-
Mouth part, 2--bag mouth, 3- mushroom culture medium.
Claims (1)
とからなり：硬質容器Ａ内に収納した軟質容器Ｂの口部
１を絞った状態で硬質容器Ａの側壁に設けた袋絞口２か
ら外に出したことを特徴とするキノコ菌糸塊培養用容器
。(1) Hard container A and soft container B filled with mushroom culture medium
and: For culturing a mushroom mycelial mass, which is characterized in that the opening 1 of a soft container B stored in a hard container A is squeezed and then taken out from the bag opening 2 provided on the side wall of the hard container A. container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62283388A JPH01124329A (en) | 1987-11-10 | 1987-11-10 | Vessel for culturing mycelial pellet of mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62283388A JPH01124329A (en) | 1987-11-10 | 1987-11-10 | Vessel for culturing mycelial pellet of mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01124329A true JPH01124329A (en) | 1989-05-17 |
Family
ID=17664871
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62283388A Pending JPH01124329A (en) | 1987-11-10 | 1987-11-10 | Vessel for culturing mycelial pellet of mushroom |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01124329A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005021703A1 (en) * | 2003-08-29 | 2005-03-10 | Yamaha Hatsudoki Kabushiki Kaisha | Microorganism culturing apparatus and method of inserting bag into culturing apparatus |
| WO2014118757A1 (en) * | 2013-02-01 | 2014-08-07 | Institut De Recherche Pour Le Developpement (Ird) | Solid state fermentation device and products obtained |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5398253A (en) * | 1977-02-09 | 1978-08-28 | Masahiro Tamura | Artificial cultivation of shiitake mushroom |
-
1987
- 1987-11-10 JP JP62283388A patent/JPH01124329A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5398253A (en) * | 1977-02-09 | 1978-08-28 | Masahiro Tamura | Artificial cultivation of shiitake mushroom |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005021703A1 (en) * | 2003-08-29 | 2005-03-10 | Yamaha Hatsudoki Kabushiki Kaisha | Microorganism culturing apparatus and method of inserting bag into culturing apparatus |
| WO2014118757A1 (en) * | 2013-02-01 | 2014-08-07 | Institut De Recherche Pour Le Developpement (Ird) | Solid state fermentation device and products obtained |
| FR3001735A1 (en) * | 2013-02-01 | 2014-08-08 | Inst Rech Developpement Ird | DEVICE FOR FERMENTATION IN SOLID MEDIUM AND PRODUCTS OBTAINED |
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