JPH0213748B2 - - Google Patents
Info
- Publication number
- JPH0213748B2 JPH0213748B2 JP17670582A JP17670582A JPH0213748B2 JP H0213748 B2 JPH0213748 B2 JP H0213748B2 JP 17670582 A JP17670582 A JP 17670582A JP 17670582 A JP17670582 A JP 17670582A JP H0213748 B2 JPH0213748 B2 JP H0213748B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- liquid sensor
- pot
- sample suction
- passes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000007788 liquid Substances 0.000 claims description 74
- 239000011148 porous material Substances 0.000 claims description 38
- 239000003085 diluting agent Substances 0.000 claims description 19
- 238000005406 washing Methods 0.000 claims description 6
- 239000000523 sample Substances 0.000 description 96
- 239000008280 blood Substances 0.000 description 14
- 210000004369 blood Anatomy 0.000 description 14
- 238000004140 cleaning Methods 0.000 description 9
- 238000001514 detection method Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 7
- 230000005856 abnormality Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000012470 diluted sample Substances 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 101100298222 Caenorhabditis elegans pot-1 gene Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、血液分析装置などの自動分析装置に
用いられる試料吸引装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a sample aspiration device used in an automatic analyzer such as a blood analyzer.
従来、血液などの試料を吸引するときのサンプ
ル量の監視は、殆どなされておらず、分析結果か
ら判定するのが主であつた。また試料吸引パイプ
を監視する方法も種々提案されている。
Conventionally, when aspirating a sample such as blood, the sample amount has hardly been monitored, and the determination has mainly been made from the analysis results. Various methods have also been proposed for monitoring sample suction pipes.
しかし、上記の試料吸引パイプを監視する方法
では、吸引時に途中で気泡が混入した場合などに
おいては、全く無抵抗であり、測定結果に重大な
誤差を与えることがあつた。また試料が少ないこ
とを検知する機能を有するものであつても、ただ
単に測定不能の信号を発するのみであり、有効な
方法とは言えないなどの種々の問題があつた。
However, in the above-mentioned method of monitoring the sample suction pipe, if air bubbles are mixed in during suction, there is no resistance at all, which may cause a serious error in the measurement results. Furthermore, even if the method has a function of detecting that there is a small amount of sample, it simply emits a signal that makes it impossible to measure, and there are various problems such as not being an effective method.
本発明は上記の諸点に鑑みなされたもので、所
定量の試料を確実に吸引し、かつ試料不足などの
異状を明確に検知することができる試料吸引装置
を提供することを目的とするものである。 The present invention was made in view of the above points, and an object of the present invention is to provide a sample suction device that can reliably aspirate a predetermined amount of sample and clearly detect abnormalities such as sample shortage. be.
上記の目的を達成するために、本発明の試料吸
引装置は、図面に示すように、駆動装置１に回動
軸２を介して回動可能に連結され試料の通る細孔
３を有する定量コツク４と、この定量コツク４の
両側に押圧して設けられ試料の通る細孔５，６お
よび希釈液の通る細孔７，８を有する第１固定コ
ツク１０および第２固定コツク１１と、第１固定
コツク１０の試料の通る細孔５に試料吸引パイプ
１２を介して接続された試料吸引ピペツト１３
と、第２固定コツク１１の試料の通る細孔６に試
料吸引パイプ１４を介して接続された試料吸引ポ
ンプ１５および洗浄液ポンプ１６と、第２固定コ
ツク１１の希釈液の通る細孔８に接続された希釈
液ポンプ１７と、第１固定コツク１０と試料吸引
ピペツト１３との間の試料吸引パイプ１２に設け
られた第１液センサ１８と、第２固定コツク１１
と試料吸引ポンプ１５との間の試料吸引パイプ１
４に設けられた第２液センサ２０と、第１液セン
サ１８に接続された第１コンパレータ２１と、第
２液センサ２０に接続された第２コンパレータ２
２と、これらのコンパレータ２１，２２に接続さ
れた判別回路２３と、この判別回路２３に接続さ
れた制御装置２４とを包含し、制御装置２４は前
記試料吸引ポンプ１５および駆動装置１に接続さ
れたことを特徴とするものである。
In order to achieve the above object, the sample suction device of the present invention, as shown in the drawings, is a quantitative metering device which is rotatably connected to a drive device 1 via a rotation shaft 2 and has a pore 3 through which a sample passes. 4, a first fixing pot 10 and a second fixing pot 11 which are pressed on both sides of the quantitative fixing pot 4 and have pores 5, 6 through which the sample passes and pores 7, 8 through which the diluent passes; A sample suction pipette 13 is connected via a sample suction pipe 12 to the pore 5 of the fixed pot 10 through which the sample passes.
A sample suction pump 15 and a washing liquid pump 16 are connected via a sample suction pipe 14 to the pore 6 of the second fixing pot 11 through which the sample passes, and are connected to the pore 8 of the second fixing pot 11 through which the diluent passes. the diluent pump 17, the first liquid sensor 18 provided on the sample suction pipe 12 between the first fixed pot 10 and the sample suction pipette 13, and the second fixed pot 11.
sample suction pipe 1 between and sample suction pump 15
4, a first comparator 21 connected to the first liquid sensor 18, and a second comparator 2 connected to the second liquid sensor 20.
2, a discrimination circuit 23 connected to these comparators 21 and 22, and a control device 24 connected to the discrimination circuit 23, the control device 24 being connected to the sample suction pump 15 and the drive device 1. It is characterized by:
第２図に示すように、駆動装置１が作動して定
量コツク４の細孔３が固定コツク１０，１１の試
料の通る細孔５，６に合うと、試料吸引ポンプ１
５により定量コツク４の細孔３内に一定量の試料
が採取される。ついで第３図に示すように、駆動
装置１により定量コツク４の細孔３が固定コツク
１０，１１の希釈液の通る細孔７，８に合うと、
希釈液ポンプ１７により希釈液が送られて、定量
コツク４の細孔３内の試料を希釈試料槽３２内に
排出する。しかる後、第４図に示すように、駆動
装置１により定量コツク４の細孔３が固定コツク
１０，１１の試料の通る細孔５，６に合うと、洗
浄液ポンプ１６が作動し洗浄液を供給してパイプ
内を洗浄し、洗浄容器３３内に排出する。
As shown in FIG. 2, when the drive device 1 is activated and the pores 3 of the metering pot 4 fit into the pores 5 and 6 of the fixed pots 10 and 11 through which the sample passes, the sample suction pump 1
5, a certain amount of sample is collected into the pore 3 of the quantitative measurement pot 4. Next, as shown in FIG. 3, when the drive device 1 aligns the pores 3 of the metering pot 4 with the pores 7 and 8 of the fixed pots 10 and 11 through which the diluent passes,
A diluent is sent by the diluent pump 17 to discharge the sample in the pore 3 of the metering pot 4 into the diluted sample tank 32. Thereafter, as shown in FIG. 4, when the drive unit 1 aligns the pores 3 of the metering pot 4 with the pores 5 and 6 of the fixed pots 10 and 11 through which the sample passes, the cleaning liquid pump 16 is activated to supply the cleaning liquid. The inside of the pipe is cleaned and discharged into the cleaning container 33.
また、第９図に示すように、制御装置２４は試
料吸引ポンプ１５および駆動装置１に接続されて
おり、最後に第１液センサ１８を通過した気泡が
第２液センサ２０を通過したときに、吸引を停止
し、定量コツク４を回動させ、希釈、洗浄を行
う。なお、詳細については〔実施例〕の項で説明
している。 Further, as shown in FIG. 9, the control device 24 is connected to the sample suction pump 15 and the drive device 1, and when the bubbles that have finally passed through the first liquid sensor 18 pass through the second liquid sensor 20, , stop the suction, rotate the metering pot 4, and perform dilution and washing. Note that details are explained in the [Example] section.
以下、本発明の実施例を図面に基づいて説明す
る。第１図は本発明の試料吸引装置の機構部を示
し、第２図〜第８図はこの機構部の動作を示して
いる。試料の吸引、希釈、系の洗浄は第２図〜第
４図のごとくに行われる。また通常の異状の検知
は第５図〜第８図のごとくに行われる。第９図は
本発明の試料吸引装置の一実施例を示し、第１０
図および第１１図はその動作を示すタイムチヤー
ト図である。
Embodiments of the present invention will be described below based on the drawings. FIG. 1 shows the mechanical section of the sample suction device of the present invention, and FIGS. 2 to 8 show the operation of this mechanical section. Aspiration of the sample, dilution, and cleaning of the system are performed as shown in FIGS. 2 to 4. Further, normal abnormality detection is performed as shown in FIGS. 5 to 8. FIG. 9 shows an embodiment of the sample suction device of the present invention, and FIG.
This figure and FIG. 11 are time charts showing the operation.
第１図〜第９図に示すように、本発明の試料吸
引装置は、エアシリンダなどの駆動装置１に回動
軸２を介して回動可能に連結され試料の通る細孔
３を有する定量コツク４と、この定量コツク４の
両側に押圧して設けられ試料の通る細孔５，６お
よび希釈液の通る細孔７，８を有する第１固定コ
ツク１０および第２固定コツク１１と、第１固定
コツク１０の試料の通る細孔５に、試料吸引パイ
プ１２を介して接続された試料吸引ピペツト１３
と、第２固定コツク１１の試料の通る細孔６に、
試料吸引パイプ１４を介して接続された試料吸引
ポンプ１５および洗浄液ポンプ１６と、第２固定
コツク１１の希釈液の通る細孔８に接続された希
釈液ポンプ１７と、第１固定コツク１０と試料吸
引ピペツト１３との間の試料吸引パイプ１２に設
けられた第１液センサ１８と、第２固定コツク１
１と試料吸引ポンプ１５との間の試料吸引パイプ
１４に設けられた第２液センサ２０と、第１液セ
ンサ１８に接続された第１コンパレータ２１と、
第２液センサ２０に接続された第２コンパレータ
２２と、これらのコンパレータ２１，２２に接続
された判別回路２３と、この判別回路２３に接続
された制御装置２４とからなり、制御装置２４は
前記試料吸引ポンプ１５および駆動装置１に接続
され、最後に第１液センサ１８を通過した気泡が
第２液センサ２０を通過したときに、吸引を停止
し、定量コツク４を回動させ、希釈、洗浄を行う
ように構成されている。試料吸引ピペツト１３の
下端は試料容器２５内の試料２６中に浸漬され、
試料を吸引して第１固定コツク１０、定量コツク
４、第２固定コツク１１からなるサンプリングバ
ルブに導くようになつている。前述のように、定
量コツク４には試料の通る細孔３があいており、
その細孔分の量だけの試料をサンプリングするよ
うになつている。２７は定圧押え具で、内部にバ
ネを有し、固定コツクを定量コツクに押えつけて
いる。回動軸２は、定量コック４とエアシリンダ
などの駆動装置１のアーム２８とに直結されてお
り、駆動装置１の動きを、アーム２８により回動
運動に変えて定量コツク４を回動させる。固定コ
ツク１０，１１は固定キー３０により回動しない
ようになつている。３１はコツクを支えるフラン
ジで、内部を回動軸２が通つている。 As shown in FIGS. 1 to 9, the sample suction device of the present invention is rotatably connected to a drive device 1 such as an air cylinder via a rotation shaft 2, and has a pore 3 through which the sample passes. a first fixing pot 10 and a second fixing pot 11 which are pressed on both sides of the metering pot 4 and have pores 5, 6 through which the sample passes and pores 7, 8 through which the diluent passes; 1 a sample suction pipette 13 connected to the sample-passing pore 5 of the fixed pot 10 through a sample suction pipe 12;
and the pore 6 of the second fixing pot 11 through which the sample passes,
A sample suction pump 15 and a washing liquid pump 16 are connected via a sample suction pipe 14, a diluent pump 17 is connected to a pore 8 of the second fixing pot 11 through which the diluent passes, and the first fixing pot 10 and the sample are connected to each other via a sample suction pipe 14. A first liquid sensor 18 provided on the sample suction pipe 12 between the suction pipette 13 and the second fixing pot 1
1 and the sample suction pump 15, a second liquid sensor 20 provided on the sample suction pipe 14, and a first comparator 21 connected to the first liquid sensor 18,
It consists of a second comparator 22 connected to the second liquid sensor 20, a discrimination circuit 23 connected to these comparators 21 and 22, and a control device 24 connected to this discrimination circuit 23. It is connected to the sample suction pump 15 and the drive device 1, and when the bubbles that have finally passed through the first liquid sensor 18 pass through the second liquid sensor 20, suction is stopped, and the metering tip 4 is rotated to dilute, It is configured to perform cleaning. The lower end of the sample suction pipette 13 is immersed in the sample 26 in the sample container 25,
The sample is aspirated and guided to a sampling valve consisting of a first fixed pot 10, a metering pot 4, and a second fixed pot 11. As mentioned above, the quantitative test tube 4 has a pore 3 through which the sample passes.
The sample is designed to be as large as the pore size. 27 is a constant pressure presser, which has a spring inside and presses the fixed pot against the metering pot. The rotation shaft 2 is directly connected to the metering cock 4 and the arm 28 of the driving device 1 such as an air cylinder, and the arm 28 converts the movement of the driving device 1 into rotational motion to rotate the metering cock 4. . The fixed tips 10 and 11 are prevented from rotating by a fixed key 30. 31 is a flange that supports the kettle, and the rotating shaft 2 passes through the inside.
第２図は試料吸引の状態を示している。すなわ
ち駆動装置１が作動して定量コツク４の細孔３が
固定コツク１０，１１の試料の通る細孔５，６に
合うと、試料吸引ポンプ１５により、定量コツク
４の細孔３内に一定量の試料が採取される。つい
で第３図に示すように、駆動装置１により定量コ
ツク４の細孔３が固定コツク１０，１１の希釈液
の通る細孔７，８に合うと、希釈液ポンプ１７に
より希釈液が送られて、定量コツク４の細孔３内
の試料を希釈試料槽３２内に排出する。しかる
後、第４図に示すように、駆動装置１により定量
コツク４の細孔３が固定コツク１０，１１の試料
の通る細孔５，６に合うと、洗浄液ポンプ１６が
作動し洗浄液を供給してパイプ内を洗浄し、洗浄
容器３３内に排出する。３４は三方弁、３５は希
釈液パイプである。 FIG. 2 shows the state of sample suction. That is, when the drive device 1 operates and the pore 3 of the metering pot 4 matches the pores 5 and 6 of the fixed pot 10 and 11 through which the sample passes, the sample suction pump 15 causes a fixed amount of water to be drawn into the pore 3 of the metering pot 4. A sample of the amount is taken. Next, as shown in FIG. 3, when the driving device 1 aligns the pores 3 of the metering pot 4 with the pores 7 and 8 of the fixed pots 10 and 11 through which the diluent passes, the diluent is sent by the diluent pump 17. Then, the sample in the pore 3 of the metering pot 4 is discharged into the diluted sample tank 32. Thereafter, as shown in FIG. 4, when the drive unit 1 aligns the pores 3 of the metering pot 4 with the pores 5 and 6 of the fixed pots 10 and 11 through which the sample passes, the cleaning liquid pump 16 is activated to supply the cleaning liquid. The inside of the pipe is cleaned and discharged into the cleaning container 33. 34 is a three-way valve, and 35 is a diluent pipe.
異状の検知は第５図〜第８図に示すように、定
量コツク４の両側に設けられた第１液センサ１８
および第２液センサ２０によつて行われる。第５
図は試料吸引後の正常な状態を示し、第１液セン
サ１８および第２液センサ２０は血液試料を検知
する。第５図に示すごとくに吸引したときの試料
の必要量は0.1mlで、定量コツク４において定量
される試料は約0.02ml程度である。第６図は試料
が少ない状態を示し、第１液センサ１８は空気、
第２液センサ２０は血液試料を検知する。第７図
は試料吸引パイプ１３の先端に詰まり（凝固）が
生じた状態を示し、第１液センサ１８は血液試料
または希釈液、第２液センサ２０は希釈液を検知
する。３６は凝固物である。第８図は試料がない
状態を示し、第１液センサ１８および第２液セサ
２０は空気を検知する。 Detection of abnormalities is performed by first liquid sensors 18 provided on both sides of the metering pot 4, as shown in FIGS. 5 to 8.
and is performed by the second liquid sensor 20. Fifth
The figure shows a normal state after sample aspiration, where the first liquid sensor 18 and the second liquid sensor 20 detect a blood sample. As shown in FIG. 5, the required amount of sample when aspirated is 0.1 ml, and the amount of sample quantified in the quantification pot 4 is about 0.02 ml. FIG. 6 shows a state where there is little sample, and the first liquid sensor 18 is connected to air,
The second liquid sensor 20 detects a blood sample. FIG. 7 shows a state where the tip of the sample suction pipe 13 is clogged (coagulated), the first liquid sensor 18 detects the blood sample or diluted liquid, and the second liquid sensor 20 detects the diluted liquid. 36 is a coagulated product. FIG. 8 shows a state in which there is no sample, and the first liquid sensor 18 and the second liquid sensor 20 detect air.
血液のような高粘度の試料は、予め均一に攪拌
され、攪拌時に生じる気泡の混入は避けられず、
混入した気泡は容易に消えない。したがつて、単
なる定量コツク両端部の空気の有無を検知するだ
けでは不充分であり、定量コツクを含む系に気泡
の混入が無いことを確認する必要がある。 High viscosity samples such as blood are stirred uniformly in advance, and the inclusion of air bubbles during stirring is unavoidable.
The mixed air bubbles do not disappear easily. Therefore, it is not sufficient to simply detect the presence or absence of air at both ends of the metering tube, and it is necessary to confirm that there are no air bubbles mixed into the system containing the metering tube.
第９図は本発明の試料吸引装置の一実施例を示
し、第１０図および第１１図はその動作を示すタ
イムチヤート図である。第１液センサ１８および
第２液センサ２０の出力は、第１コンパレータ２
１および第２コンパレータ２２によつて、たとえ
ば血液試料と空気との透過光量の差により、基準
電圧との比較を行い、１、０の出力差を生ずる。
この出力Q1、Q2は判別回路２３に送られ、気泡
がないことを確認して試料吸引ポンプ１５を停止
し、駆動装置１を駆動して定量コツク４を回動し
希釈が行われる。これは以下のごとくに行われ
る。 FIG. 9 shows an embodiment of the sample suction device of the present invention, and FIGS. 10 and 11 are time charts showing its operation. The outputs of the first liquid sensor 18 and the second liquid sensor 20 are connected to the first comparator 2.
The first and second comparators 22 perform a comparison with a reference voltage based on the difference in the amount of transmitted light between a blood sample and air, for example, and produce an output difference of 1 and 0.
These outputs Q 1 and Q 2 are sent to the discrimination circuit 23, and after confirming that there are no bubbles, the sample suction pump 15 is stopped, and the drive device 1 is driven to rotate the metering pot 4 to perform dilution. This is done as follows.
まず第１０図に示すように、吸引初めの部分に
のみ気泡が混入している場合には、判別回路２３
から制御装置２４へ送られる制御信号Ｓは、第２
液センサ２０に血液→気泡→血液という具合に気
泡が抜けたときに初めて発せられるが、第１１図
に示すように、この初めの部分が第２液センサ２
０に達する前に、再び気泡が混入したときには、
改めて最も新しい気泡の第２液センサ２０の通過
を待つて、制御信号Ｓが発せられる。したがつ
て、吸引時間に多少のずれを生ずるが、全体で約
0.1mlの試料が吸引され、センサー間の試料は全
体の1/5程度であるので、それが初めの部分にな
るか最後の部分になるかは、あまり問題とはなら
ない。また制御信号Ｓが発せられた後に、新しい
気泡が第１液センサ１８を通過しかけても、第１
液センサ１８、第２液センサ２０の間の大部分に
は気泡が混入しておらず問題にはならない。全体
の吸引時間は、制御装置２４に内蔵されたタイマ
ーによつて行われ、約0.1mlの試料吸引時間に相
当する時間を過ぎてもまだ気泡が混入している場
合には、異常状態として警報が発せられるように
構成されている。 First, as shown in FIG. 10, if air bubbles are mixed only at the beginning of suction, the discrimination circuit 2
The control signal S sent from the second
It is first emitted when air bubbles are removed from the liquid sensor 20 in the following order: blood → air bubbles → blood. As shown in FIG.
If air bubbles are mixed in again before reaching 0,
After waiting for the newest bubble to pass through the second liquid sensor 20, the control signal S is issued. Therefore, there will be some deviation in the suction time, but the overall difference will be approximately
A 0.1 ml sample is aspirated, and the sample between the sensors is about 1/5 of the total, so it doesn't really matter whether it is the first part or the last part. Furthermore, even if a new bubble is about to pass through the first liquid sensor 18 after the control signal S is issued, the first liquid sensor 18
Most of the air bubbles are not mixed in between the liquid sensor 18 and the second liquid sensor 20, so there is no problem. The entire aspiration time is determined by a timer built into the control device 24, and if air bubbles are still mixed in after the time equivalent to approximately 0.1 ml of sample aspiration time, an alarm is issued as an abnormal condition. is configured so that it is emitted.
第１０図および第１１図について、さらに詳細
に説明する。第１０図において、第１液センサ１
８を通過した気泡は１個であり、その気泡は第２
液センサ２０を通過した気泡と対応している。 FIG. 10 and FIG. 11 will be explained in more detail. In FIG. 10, the first liquid sensor 1
The number of bubbles that passed through 8 is one, and that bubble is the second bubble.
This corresponds to the bubbles that have passed through the liquid sensor 20.
第１１図においては、第１液センサ１８、第２
液センサ２０を通過した気泡はそれぞれ２個であ
り、第１液センサ１８の１個目（最初）の気泡と
第２液センサ２０の１個目（最初）の気泡とが対
応し、第１液センサ１８の２個目（最後）の気泡
と第２液センサ２０の２個目（最後）の気泡とか
が対応している。 In FIG. 11, the first liquid sensor 18, the second
The number of bubbles that passed through the liquid sensor 20 is two each, and the first (first) bubble of the first liquid sensor 18 corresponds to the first (first) bubble of the second liquid sensor 20. The second (last) bubble of the liquid sensor 18 corresponds to the second (last) bubble of the second liquid sensor 20, etc.
第２液センサ２０を通過した気泡が、第１液セ
ンサ１８を最後に通過した気泡であるか否かは、
例えば、第１液センサ１８を通過した気泡の数と
第２液センサ２０を通過した気泡の数を比べるこ
とにより容易に知ることができる。両者が同数で
あれば、第１液センサ１８を通過した気泡がすべ
て第２液センサ２０から抜け出たとみなすことが
できる。第２液センサ２０を通過した気泡の方が
少なければ、気泡は抜けきつていないと見ること
ができる。 Whether the bubble that passed through the second liquid sensor 20 is the bubble that last passed through the first liquid sensor 18 is determined by
For example, it can be easily determined by comparing the number of bubbles that have passed through the first liquid sensor 18 and the number of bubbles that have passed through the second liquid sensor 20. If both numbers are the same, it can be considered that all the bubbles that have passed through the first liquid sensor 18 have escaped from the second liquid sensor 20. If fewer bubbles have passed through the second liquid sensor 20, it can be concluded that the bubbles have not completely escaped.
試料吸引量は予め決まつているので、ある程度
の量以上試料が吸引された後、第１液センサ１８
に気泡が来れば、その気泡は第２液センサ２０か
ら到底抜け出ることはできない。その場合には、
気泡の計数等を中止し、異状としてしまつてもか
まわない。したがつて、第１液センサ１８の気泡
はその状態になるまでの期間、計数されればよ
い。 Since the sample suction amount is predetermined, after a certain amount of sample has been suctioned, the first liquid sensor 18
If air bubbles arrive at the second liquid sensor 20, the air bubbles cannot escape from the second liquid sensor 20. In that case,
It is okay to stop counting the bubbles and treat it as an abnormality. Therefore, the bubbles in the first liquid sensor 18 need only be counted during the period until this state is reached.
第１液センサ１８の気泡の計数期間は、試料の
吸引量、吸引速度、試料吸引ピペツト１３の先端
から第１液センサ１８までの容積、第１液センサ
１８から第２液センサ２０までの容積等の条件の
違いによつて、適当に決めればよい。例を上げれ
ば、試料吸引ポンプ１５が動作し始めてから所定
時間まで、第１液センサ１８に血液が来てから所
定時間まで、又は第１液センサ１８に血液が来て
から第２液センサに血液が来るまで、等のように
条件に応じて決めることができる。第１液センサ
１８に血液が来てから第２液センサ２０に血液が
来るまでを計数期間とすると、試料の粘性に関係
なく、一定量の試料が吸引されるまでを、計数期
間とすることができるので、判定の安定性が向上
する。 The bubble counting period of the first liquid sensor 18 is based on the sample suction amount, the suction speed, the volume from the tip of the sample suction pipette 13 to the first liquid sensor 18, and the volume from the first liquid sensor 18 to the second liquid sensor 20. It may be determined appropriately depending on the differences in conditions such as. For example, after the sample suction pump 15 starts operating until a predetermined time, after blood arrives at the first liquid sensor 18 and until a predetermined time, or after blood arrives at the first liquid sensor 18 and until the second liquid sensor It can be determined depending on the conditions, such as until blood arrives. If the counting period is defined as the period from when blood arrives at the first liquid sensor 18 until the blood arrives at the second liquid sensor 20, the counting period is defined as the period until a certain amount of sample is aspirated, regardless of the viscosity of the sample. This improves the stability of judgment.
本発明の試料吸引装置は、上記のように気泡の
検知をコツクの切替時に行うのではなく、気泡の
通過を時間的にとらえ、系に気泡が無いことを確
認して行うように構成されているので、試料不足
などの異状を明確に検知し、定量ミスが生じない
という効果を有している。
The sample suction device of the present invention is configured to detect air bubbles not at the time of switching between the bubbles as described above, but to detect the passage of air bubbles over time to confirm that there are no air bubbles in the system. This has the effect of clearly detecting abnormalities such as sample shortages and preventing quantitative errors.
第１図は本発明の試料吸引装置の機構部の一例
を示す斜視図、第２図は試料を吸引している状態
を示す説明図、第３図は希釈状態を示す説明図、
第４図は洗浄状態を示す説明図、第５図は正常な
場合におけるセンサの検知状態を示す説明図、第
６図は試料が少ない場合におけるセンサの検知状
態を示す説明図、第７図は試料吸引ピペツトなど
に詰まり（凝固）が生じた場合におけるセンサの
検知状態を示す説明図、第８図は試料がない場合
におけるセンサの検知状態を示す説明図、第９図
は本発明の試料吸引装置の一実施例を示す説明
図、第１０図および第１１図は動作を示すタイム
チヤート図である。
１……駆動装置、２……回動軸、３……試料の
通る細孔、４……定量コツク、５，６……試料の
通る細孔、７，８……希釈液の通る細孔、１０…
…第１固定コツク、１１……第２固定コツク、１
２……試料吸引パイプ、１３……試料吸引ピペツ
ト、１４……試料吸引パイプ、１５……試料吸引
ポンプ、１６……洗浄液ポンプ、１７……希釈液
ポンプ、１８……第１液センサ、２０……第２液
センサ、２１……第１コンパレータ、２２……第
２コンパレータ、２３……判別回路、２４……制
御装置、２５……試料容器、２６……試料、２７
……定圧押え具、２８……アーム、３０……固定
キー、３１……フランジ、３２……希釈試料槽、
３３……洗浄容器、３４……三方弁、３５……希
釈液パイプ、３６……凝固物。
FIG. 1 is a perspective view showing an example of the mechanical part of the sample suction device of the present invention, FIG. 2 is an explanatory view showing a state in which a sample is being aspirated, and FIG. 3 is an explanatory view showing a diluted state.
Fig. 4 is an explanatory diagram showing the cleaning state, Fig. 5 is an explanatory diagram showing the sensor detection state in a normal case, Fig. 6 is an explanatory diagram showing the sensor detection state when there is little sample, and Fig. 7 is an explanatory diagram showing the sensor detection state in a normal case. An explanatory diagram showing the detection state of the sensor when clogging (coagulation) occurs in the sample suction pipette, etc., Fig. 8 is an explanatory diagram showing the detection state of the sensor when there is no sample, and Fig. 9 shows the sample suction of the present invention. An explanatory diagram showing one embodiment of the apparatus, and FIGS. 10 and 11 are time charts showing the operation. 1... Drive device, 2... Rotating shaft, 3... Pore through which the sample passes, 4... Quantitative pot, 5, 6... Pore through which the sample passes, 7, 8... Pore through which the diluent passes. , 10...
...First fixed pot, 11...Second fixed pot, 1
2... Sample suction pipe, 13... Sample suction pipette, 14... Sample suction pipe, 15... Sample suction pump, 16... Washing liquid pump, 17... Diluent pump, 18... First liquid sensor, 20 ... Second liquid sensor, 21 ... First comparator, 22 ... Second comparator, 23 ... Discrimination circuit, 24 ... Control device, 25 ... Sample container, 26 ... Sample, 27
... Constant pressure holder, 28 ... Arm, 30 ... Fixed key, 31 ... Flange, 32 ... Diluted sample tank,
33... Washing container, 34... Three-way valve, 35... Diluent pipe, 36... Coagulated material.
Claims (1)
結され試料の通る細孔３を有する定量コツク４
と、この定量コツク４の両側に押圧して設けられ
試料の通る細孔５，６および希釈液の通る細孔
７，８を有する第１固定コツク１０および第２固
定コツク１１と、第１固定コツク１０の試料の通
る細孔５に試料吸引パイプ１２を介して接続され
た試料吸引ピペツト１３と、第２固定コツク１１
の試料の通る細孔６に試料吸引パイプ１４を介し
て接続された試料吸引ポンプ１５および洗浄液ポ
ンプ１６と、第２固定コツク１１の希釈液の通る
細孔８に接続された希釈液ポンプ１７と、第１固
定コツク１０と試料吸引ピペツト１３との間の試
料吸引パイプ１２に設けられた第１液センサ１８
と、第２固定コツク１１と試料吸引ポンプ１５と
の間の試料吸引パイプ１４に設けられた第２液セ
ンサ２０と、第１液センサ１８に接続された第１
コンパレータ２１と、第２液センサ２０に接続さ
れた第２コンパレータ２２と、これらのコンパレ
ータ２１，２２に接続された判別回路２３と、こ
の判別回路２３に接続された制御装置２４とを包
含し、制御装置２４は前記試料吸引ポンプ１５お
よび駆動装置１に接続されたことを特徴とする試
料吸引装置。1. A metering pot 4 which is rotatably connected to the drive device 1 via a rotation shaft 2 and has a pore 3 through which the sample passes.
, a first fixing pot 10 and a second fixing pot 11 which are pressed on both sides of the quantitative fixing pot 4 and have pores 5, 6 through which the sample passes and pores 7, 8 through which the diluent passes; A sample suction pipette 13 connected to the small hole 5 of the pot 10 through which the sample passes through a sample suction pipe 12, and a second fixing pot 11.
A sample suction pump 15 and a washing liquid pump 16 are connected to the pore 6 through which the sample passes, via a sample suction pipe 14, and a diluent pump 17 is connected to the pore 8 through which the diluent of the second fixing pot 11 passes. , a first liquid sensor 18 provided on the sample suction pipe 12 between the first fixed pot 10 and the sample suction pipette 13.
, a second liquid sensor 20 provided on the sample suction pipe 14 between the second fixed pot 11 and the sample suction pump 15, and a first liquid sensor 20 connected to the first liquid sensor 18.
It includes a comparator 21, a second comparator 22 connected to the second liquid sensor 20, a discrimination circuit 23 connected to these comparators 21 and 22, and a control device 24 connected to this discrimination circuit 23, A sample suction device characterized in that a control device 24 is connected to the sample suction pump 15 and the drive device 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17670582A JPS5965769A (en) | 1982-10-07 | 1982-10-07 | Sample suction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17670582A JPS5965769A (en) | 1982-10-07 | 1982-10-07 | Sample suction device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5965769A JPS5965769A (en) | 1984-04-14 |
| JPH0213748B2 true JPH0213748B2 (en) | 1990-04-05 |
Family
ID=16018304
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17670582A Granted JPS5965769A (en) | 1982-10-07 | 1982-10-07 | Sample suction device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5965769A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007064680A (en) * | 2005-08-29 | 2007-03-15 | Sysmex Corp | Liquid sample suction monitoring method, device, and liquid sample analyzer |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6220575B2 (en) | 2013-06-27 | 2017-10-25 | シスメックス株式会社 | Sample processing apparatus and abnormality detection method for sample processing apparatus |
-
1982
- 1982-10-07 JP JP17670582A patent/JPS5965769A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007064680A (en) * | 2005-08-29 | 2007-03-15 | Sysmex Corp | Liquid sample suction monitoring method, device, and liquid sample analyzer |
| JP4593404B2 (en) * | 2005-08-29 | 2010-12-08 | シスメックス株式会社 | Liquid sample suction monitoring method and apparatus, and liquid sample analyzer |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5965769A (en) | 1984-04-14 |
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