JP2867670B2 - Automatic chemical analysis method and apparatus - Google Patents

Description

The present invention relates to an automatic chemical analysis method and an apparatus therefor, and more particularly, to an automatic chemical analysis method using an automatic chemical analysis apparatus, wherein a reagent container which is out of reagent is used. The present invention relates to an automatic chemical analysis method and an automatic chemical analysis method capable of replacing a reagent container containing a reagent without stopping the automatic chemical analysis device.

Further, the present invention, for example, blood, plasma, serum, bodily fluids such as lymph, excretions such as urine, gastric juice, pancreatic juice, bile, saliva,
The present invention relates to a system for replenishing reagents used in an automatic chemical analyzer by a spectrophotometry method for a liquid sample such as a specimen such as secretion fluid such as sweat, ascites, pleural effusion, or a puncture fluid such as joint cavity fluid.

(B) Conventional technology For example, in a discrete-type automatic chemical analyzer such as a turntable type or a conveyor type, a plurality of reaction vessels capable of measuring absorbance, that is, a plurality of reaction vessels are provided so that intermittent movement is possible. A formed turntable or conveyor device is provided, and around the movement path of the reaction vessel, a sample can be dispensed and a reagent can be dispensed into the reaction vessel, and the reaction vessel can be washed and measured for absorbance. , A sample dispensing device, a reagent dispensing device, a washing device and a measuring device, and an analysis operation control device for controlling the operation of these devices.

When performing analysis on one or more analysis items for each of a plurality of samples by such an automatic chemical analyzer, for example, a reaction vessel such as a reaction cuvette capable of measuring absorbance is placed on a turntable along a reaction line. And a plurality of the turntables are intermittently or continuously rotated according to a preset time program by the analysis operation control device, and each reaction vessel is, for example, sequentially, a sample dispensing area, a reagent The transfer is performed in the order of the dispensing area, the stirring area, the reaction area and the washing area, and when the turntable is stopped, the sample dispensing area, the reagent dispensing area, the stirring area, the reaction area and the reaction vessel located in the washing area, respectively, Sample dispensing,
Perform each analysis operation such as reagent dispensing, stirring of samples and reagents, reaction, and washing of the reaction container.For the sample located in the measurement area, use the rate method or the endpoint method to analyze the analysis item components for each reaction container. The absorbance is measured, then the turntable is rotated intermittently, and the reaction vessel is sent to the next stop position, and for the reaction vessels located in each area, the sample dispensing and the reagent dispensing are performed similarly to the previous time. , Agitation, reaction and measurement, and washing of the reaction vessel, and the analysis is carried out repeatedly and continuously.

By the way, in an automatic chemical analyzer, reagents used for analysis are arranged on a reagent mounting table of a reagent dispensing apparatus, and reagent containers each containing a reagent are placed therein. Is aspirated and dispensed into the reaction vessel.

However, the amount of reagent consumption depends on the frequency of use of the reagent, and the number of times of analysis differs for each analysis item according to the necessity of the analysis. Reagents are used more frequently and will run out during analysis faster than other reagents that are analyzed less frequently.

Although the capacity of the reagent container is increased in accordance with the frequency of use of such a reagent, the size of the reagent container is limited, and thus running out of the reagent cannot be avoided. Therefore, a spare reagent container is provided for a frequently used reagent to prepare for a case where the used reagent container runs out of reagent.

Therefore, when the reagent runs out as described above, the operation of replacing the reagent container that has run out of the reagent with a new reagent container containing the reagent is performed. Dispensing reagents,
Subsequent samples are being analyzed.

(C) Problems to be Solved by the Invention By the way, analysis of analysis items in an automatic chemical analyzer is performed by reacting a sample to be analyzed with a reagent and measuring the absorbance of a reaction product. Since the remaining reagent exists in addition to the reaction product and the measured absorbance value is affected by the reagent blank value and the calibration curve constant or the reagent activity, the reagent blank value and the calibration curve The analysis value is calculated by measuring the constant in advance and correcting the measured value of the absorbance of the sample.

Therefore, in the automatic chemical analyzer, when the used reagent runs out of reagent, even if the spare reagent is dispensed from the spare reagent container, the reagent blank value and Calibration curve constants must be determined.

However, in the case of a multi-item automatic chemical analyzer, when the used reagent runs out of reagents, when the reagent is dispensed by switching from a reagent container that has run out of reagents to a reagent container that contains a spare reagent, the reaction line contains no reagent. Along with the reaction container in which the sample of another analysis item that does not use the out-of-sample reagent is dispensed, the reaction container in which the reagent has already been dispensed before the out-of-reagent is arranged without completing the analysis. Before the determination of the reagent blank value and the calibration curve constant for the reaction container, it is necessary to operate the automatic chemical analyzer and measure the absorbance of the reaction container in which the reagent has already been dispensed.

Therefore, while analysis is performed on a reaction container in which those reagents have already been dispensed, analysis on a new sample cannot be performed. However, when the analysis is switched to the dispensing, the amount of analysis processing is reduced due to the stoppage of the analysis of the new sample, which is a problem.

SUMMARY OF THE INVENTION The present invention relates to a problem with a conventional automatic chemical analyzer, in which analysis of a new sample is stopped when switching the collection of a reagent for dispensing a reagent from a reagent container that has run out of reagent to a reagent in a spare reagent container. It is intended to solve.

(D) Means for Solving the Problems The present invention relates to an automatic chemical analyzer, which switches a reagent collection for dispensing a reagent from a reagent container that has run out of reagent to a reagent in a spare reagent container. It is an object of the present invention to provide an automatic chemical analyzer capable of analyzing a new sample without stopping the operation of the sample dispensing device.

The present invention relates to an automatic chemical analysis method in which a reagent collection is switched from a used reagent container to a spare reagent container containing the reagent, and analysis is performed by dispensing a spare reagent from the spare reagent container. From the measured absorbance, a provisional analysis value is obtained by substituting the analysis value calculation constant of the used reagent that has run out of the reagent as the analysis value calculation constant of the preliminary reagent used in the analysis, and then, for each of the calibration samples, Calculate the analytical value calculation constant of the preliminary reagent from the measured absorbance by dispensing, and correct the tentatively determined analytical value by the analytical value calculation constant of the preliminary reagent. In the method, the present invention provides a reagent dispensing apparatus and a measuring apparatus in which a reaction line is formed and a sample dispensing device, a used reagent container and a spare reagent container are provided along the reaction line. An analysis operation control device for controlling the operation of the apparatus is provided.At the time of analysis, the reagent is dispensed while monitoring the remaining amount of the reagent in the used reagent container, and when the remaining amount of the reagent reaches a predetermined amount, In an automatic chemical analyzer that dispenses a spare reagent from a spare reagent container to a reaction container arranged in a reaction line, an analysis operation control device monitors a remaining amount of a reagent in each reagent container, and a reagent remaining amount management unit. An analysis item determining unit that receives a signal from the reagent remaining amount management unit to determine whether an analysis item uses a spare reagent; and an analysis unit that receives a signal from the analysis item determination unit and analyzes the used spare reagent. As a value calculation constant, an analysis data management unit that continuously uses the analysis value calculation constant of the used reagent that has run out of the reagent before using the spare reagent and calculates the analysis value with a correction signal,
Dispensing a calibration sample into a reaction container, measuring the absorbance of the reaction container, and operating a sample dispensing unit, a reagent dispensing unit and a correction reagent calibration unit that operates the measuring unit to obtain an analysis value calculation constant of the preliminary reagent, An automatic chemical analyzer comprising: an analytical value correction operation unit that corrects an analytical value added with the calculated correction signal by a correction operation signal of an analytical value calculation constant from the correction reagent calibration unit. is there.

In the present invention, the term “analysis value calculation constant of a reagent” means a reagent blank value of a reagent or a calibration curve constant of a reagent required for calculation of an analysis value. When both the value and the calibration curve constant are required, it means the reagent blank value and the calibration curve constant of the reagent.

In the present invention, the automatic chemical analyzer, like the conventional automatic chemical analyzer, has a reaction line formed by arranging a plurality of reaction vessels, and along the reaction line, a sample dispenser, a reagent dispenser, and the like. A reaction device such as an injection device, a thermostat, a reaction vessel washing device, and an absorbance measurement device are provided, and these devices are controlled by an analysis operation control device according to a program.

In the present invention, the measurement of the analytical value calculation constant for the spare reagent, that is, the measurement of the reagent blank value and / or the calibration curve constant, is the measurement of the analytical value calculation constant performed in a conventional automatic chemical analyzer, that is, Similarly to the measurement of the reagent blank value and / or the measurement of the calibration curve constant, the measurement is performed by an absorbance measurement device provided along the reaction line.

That is, in the present invention, the measurement of the reagent blank for the preliminary reagent is performed by injecting water instead of the sample in the sample dispensing region into the reaction container, dispensing the reagent into the reaction container containing water, A value obtained by measuring the absorbance and subtracting a water blank value from the measured value is used as a reagent blank value. In addition, for the measurement of the calibration curve constant of the preliminary reagent, a sample of known concentration is dispensed in the sample dispensing area into the reaction vessel, and the medical treatment is performed on the sample of known concentration by the analysis procedure of the analysis item, and the absorbance is measured. It is determined by measuring.

In the present invention, the absorbance measuring device can be provided in a mobile format or a fixed format, similarly to a conventional automatic chemical analyzer.

In the present invention, the analytical value calculation constant, that is, the reagent blank value and / or the calibration curve constant has not been determined for the preliminary reagent, but in general, the preliminary reagent is prepared from a part of the reagent used, The reagent blank value and / or the calibration curve constant of the reagent do not fluctuate significantly as compared with the used reagent that has been exhausted.

Therefore, in the present invention, with respect to the reagent blank value and / or the calibration curve constant of the preliminary reagent, the provisional analysis value is temporarily determined using the reagent blank value and / or the calibration curve constant of the reagent having run out of the reagent. Before the exhaustion of the spare reagent, the reagent blank value and / or the calibration curve constant of the spare reagent is determined by actual measurement, and the sample analyzed by the spare reagent is determined by the measured reagent blank value and / or the calibration curve constant. Is temporarily corrected.
The provisional analysis values here need not necessarily be displayed.

In the present invention, a sample having a known concentration is a standard sample, a control sample, a sample that has already been analyzed and the concentration of the analysis item has been determined, or a sample that has been analyzed with a spare reagent. Examples include samples analyzed using reagents with known blank values and / or calibration curve constants.

In the present invention, the correction of the analytical value is performed by a value obtained by multiplying the absorbance obtained by subtracting the reagent blank value from the absorbance measured using the spare reagent by a calibration curve constant.

In the present invention, the analysis items of the sample for which the analysis values are tentatively determined are, for example, marked with special marks indicating the necessity of examination, correction, etc., the reagent blank value of the preliminary reagent and the calibration curve constant. Are corrected all at once when is requested.

Therefore, the automatic chemical analyzer of the present invention is provided with an analysis operation control device. The analysis operation control device includes a reagent remaining amount management unit for monitoring the reagent exhaustion, an analysis item determination unit for determining an analysis item for which a spare reagent is to be used, and a sample for which the spare reagent has been dispensed. The measured absorbance is used as the analytical value calculation constant of the reagent run out, ie, the reagent blank value and / or calibration curve constant of the reagent as the analytical value calculation constant of the spare reagent, ie, the reagent blank value and / or calibration curve constant. Analytical data management unit that calculates tentative analytical values in place of substitutes, for the spare reagent, controls the operation of the sample dispensing unit and the reagent dispensing unit to obtain the reagent blank value and the calibration curve constant. That is, samples having known concentrations of water and / or analysis item components were separately dispensed into reaction cuvettes, respectively, and were determined by a reagent calibration section for a preliminary reagent for measuring absorbance, and the reagent calibration section. Reagent Based on the link value and the calibration curve constants, and includes an analysis value correction operation unit for performing provisionally fixes calculated analytical values using a pre-reagent.

(E) Function The present invention switches the reagent collection from the used reagent container to the spare reagent container when the used reagent runs out of the reagent, and calculates the analysis value calculation constant of the used reagent that has run out of the reagent, that is, the reagent blank value and / or Alternatively, the tentative analysis value is obtained by substituting the calibration curve constant for the reagent blank value and / or the calibration curve constant of the preliminary reagent, and the reagent blank value and / or the calibration curve constant of the preliminary reagent is used during the use of the preliminary reagent. Since the measurement is performed and the provisional analysis value is corrected, even if the used reagent runs out of reagent, it is possible to immediately shift to the spare reagent, and there is no hindrance to the analysis operation of the automatic chemical analyzer. In addition, while the dispensing with the spare reagent is being performed, the spare reagent can be calibrated and accurate analysis values can be calculated without hindering the analysis operation of the automatic chemical analyzer. Replenishment of reagents can be performed.

Therefore, in the present invention, even if the reagent runs out, it is possible to switch to a new used reagent while continuing the analysis work of the automatic chemical analyzer, and to calibrate the spare reagent without using a standard sample. Is economical.

Further, according to the present invention, each time the reagent of the automatic chemical analyzer is newly replaced, it can be compared with the analysis value of the previous reagent, so that the accuracy of the analysis value can be monitored.

(F) Examples Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings, but the present invention is not limited by the following description and examples.

FIG. 1 is a schematic explanatory view mainly showing a sample dispensing section, a reagent dispensing section and a control section in an automatic chemical analyzer according to one embodiment of the present invention, and FIG. 2 is shown in FIG. FIG. 3 is a schematic block diagram of an embodiment according to the present invention, and FIG. 3 is a flowchart of calibration of a preliminary reagent in one embodiment of the present invention.

In FIG. 1, the automatic chemical analyzer 1 is provided with a reaction table 2 having a cuvette holder, similarly to the conventional automatic chemical analyzer, and similarly, the cuvette holder 2 has a reaction cuvette 3 ( (Partially omitted and not shown) is attached to form a reaction line 4.

Along with the reaction line 4, a sample dispensing unit 7 including a sample dispensing device 5 and a sample table 6 is provided. A reagent dispensing unit 11 including a dispensing device 9 and a reagent table 10 is provided, and a cleaning unit including a washing device and an absorbance measuring device (downstream) in the traveling direction 8 of the reaction table 2 from the reagent dispensing unit 11 are provided. (Neither is shown).

In this example, the reaction cuvette 3 from the washing unit is sent to the separation distance of the center of the reaction cuvette adjacent to the reaction cuvette 3 arranged in the reaction line 4 by intermittent feeding of the reaction table 2. The reaction cuvette 3 sent to the sample dispensing position 12 reciprocates the sample dispensing nozzle 13 movable in the vertical direction of the sample dispensing device 5 in a direction indicated by a dashed line 14 and A sample is suctioned and collected from the sample container 16 located at the sample collection position 15, and the sample is dispensed at the sample dispensing position 12 as it is.
Then, the sample is dispensed to the reaction cuvette 3 located at the sample dispensing position 12.

The reaction cuvette 3 into which the sample has been dispensed moves in the traveling direction 8 (arrow) of the reaction table 2, and the reaction cuvette 3 sent to the reagent dispensing position 17 moves in the vertical direction of the reagent dispensing device 9. The possible reagent dispensing nozzle 18 is reciprocated in the direction indicated by the dashed line 19, and the reagent table 10 is rotated in any direction of the arrow 20, to the reagent collection position 21,
The reagent container 22 of the reagent to be dispensed is positioned, the reagent is suctioned and collected from the reagent container 22 located at the reagent collection position, and the reagent is moved to the reagent dispensing position 17 as it is, and is moved to the reagent dispensing position 17. The reagent is dispensed into the reaction cuvette 3 to be performed.

As shown in FIG. 2, in this example, the interfaces connected to the microcomputer include a sampler control computer, a reaction unit control computer, an A / D conversion / Log conversion unit (not shown), and a reagent remaining amount management. It is connected to a program, a sample data memory of known concentration, a concentration (correction) calculation unit, a dispenser control computer, a floppy disk drive, a CRT, a keyboard and a printer, and is configured to send signals from the microcomputer to the respective blocks. ing.

The fact that the reagent container 22 does not contain a sufficient amount of reagent for dispensing the reagent, that is, the reagent has run out, is detected by the reagent remaining amount management program, and this detection signal is sent from the microcomputer to the sample dispensing apparatus. The sampler control computer 5 and the dispenser control computer 9 of the reagent dispensing device 9 are sent via the interface.

The sampler control computer of the sample dispensing apparatus 5 receives the detection signal and changes the operation of the sample table 6, the pipettor pump, and the diluter pump so as to obtain the reagent blank value and the calibration curve constant of the spare reagent.

First, in order to measure the reagent blank value of the preliminary reagent,
By operating only the diluter pump (not shown), water is drawn from the sample dispensing nozzle 13 to the sample suction and collection position 15.
Dispense into reaction cuvette 3 located at Next, in order to measure the calibration curve constant of the preliminary reagent, the sample table 6 is intermittently driven in the direction of arrow 23, and the sample container 24 containing the sample of known concentration is moved to the sample aspirating and sampling position 15, and the sample concentration is measured. A sample with a known concentration is suctioned from the sample container 24 containing the known sample, and the reaction cuvette 3 located at the sample dispensing position 12 is sampled.
Dispense a sample of known concentration. At the sample dispensing position 5, after dispensing a sample with a known concentration, the subsequent sample is dispensed.

On the other hand, in response to the detection signal, the dispenser control computer of the reagent dispensing device 9 does not collect the reagent from the reagent container 22 that has run out of the reagent, but collects the reagent from the reagent container 25 containing the spare reagent. Controls the movement and operation of the dispenser pump.

The concentration (correction) calculation unit uses the detection signal of the reagent remaining amount management program sent from the microcomputer to calculate the absorbance of the sample from which the spare reagent has been dispensed, from the A / D conversion / Log conversion unit sent from the microcomputer. For the measurement data, substitute the reagent blank value and calibration curve constant of the reagent that has run out of reagent as the reagent blank value and calibration curve constant of the spare reagent, perform arithmetic processing, attach a correction required mark, and use the CRT and printer. Is displayed.

When the absorbance is measured for the reaction cuvette 3 into which the water has been dispensed, the absorbance measurement data of the water is sent from the microcomputer to the concentration (correction) calculation unit via the interface, and the reagent blank value of the preliminary reagent is used. Registered as When the absorbance was measured for the reaction cuvette 3 into which the sample of known concentration was dispensed,
The absorbance measurement data for the sample of known concentration is sent from the microcomputer to the concentration (correction) calculation unit via the interface, and the calibration curve constant of the spare reagent is calculated by calculation. be registered. When the reagent blank value and the calibration curve constant of the spare reagent were determined, the analysis value with the required correction mark obtained using the reagent blank value and the calibration curve constant of the used reagent due to lack of reagent was added. Analytical values are determined using the reagent blank values and the calibration curve constants, and the provisional analytical values are corrected.

In this example, these results are displayed on the CRT,
Printed by printer.

FIG. 3 shows a flowchart of a program of the microcomputer in this example.

(1) Determine whether or not a reagent blank measurement is necessary. When a reagent blank measurement is required, the sampler control computer is instructed to dispense water for reagent blank measurement.

(2) Next, for example, it is determined whether or not it is necessary to measure the calibration curve constant of the reagent using a standard sample.

When measurement of the calibration curve constant is required, the sampler control computer is instructed to dispense a standard sample for measuring the calibration curve constant.

(3) Analysis of the control sample or sample is started.
In dispensing the reagent, it is determined whether or not the used reagent is sufficient to dispense the reagent. If the amount of the used reagent is sufficient to dispense the reagent, the reagent is dispensed from the used reagent.

(4) If the amount of the used reagent is not enough to dispense the reagent, it is determined whether or not there is a spare reagent. When there is no spare reagent, an alarm is issued to notify that the reagent has run out.

(5) When there is a spare reagent, the judgment of (1) is performed.

(6) It is determined whether there is a control sample or a sample to be analyzed. When there is no control sample or sample to be analyzed, the analysis operation of the automatic chemical analyzer ends. When there is a control sample and a sample to be analyzed, the analysis is performed subsequently.

In this example, even if the reagent runs out, the analysis can be continued without stopping the analysis operation of the automatic chemical analyzer.

In this example, the provisional analysis value obtained using the reagent blank value and the calibration curve constant of the used reagent due to the lack of the reagent is configured to be displayed on the CRT, but is not necessarily displayed on the CRT or the like. It does not need to be done.

In this example, the analysis value is obtained using both the reagent blank value and the calibration curve constant. However, when only the reagent blank value is used as the analysis value calculation constant, the analysis is performed by measuring the reagent blank value. Value, and
When only the calibration curve constant is used as the analysis value calculation constant, the analysis value can be obtained by measuring the calibration curve constant.

(G) Effect of the Invention The present invention provides an analysis value calculation constant for a used reagent that has run out of reagent, that is, a reagent blank value, by switching reagent collection from a used reagent container to a spare reagent container when a used reagent runs out of reagent. And / or the calibration curve constant is used as the analytical value calculation constant of the preliminary reagent, that is, as a reagent blank value and / or a calibration curve constant, to tentatively determine an analytical value, and during use of the preliminary reagent,
Measures the reagent blank value and / or calibration curve constant of the preliminary reagent and corrects the provisional analysis value, so that the analysis work of each operation unit is not stopped compared to the conventional automatic chemical analyzer, The analysis of the analysis item can be continued, so that the analysis throughput does not decrease.

Further, in the present invention, every time a new reagent is replaced due to a shortage of reagent, the analysis value is extracted and compared with the analysis value of the previous reagent. This is performed when an abnormality in the analysis value or the like is detected. Compared with a conventional automatic chemical analyzer, the use of expensive standard reagents can be avoided as much as possible, so that the analysis cost can be reduced. .

[Brief description of the drawings]

FIG. 1 is a schematic explanatory view mainly showing a sample dispensing section, a reagent dispensing section, and a control section in an automatic chemical analyzer according to one embodiment of the present invention, and FIG. 2 is shown in FIG. FIG. 3 is a schematic block diagram of an embodiment according to the present invention, and FIG. 3 is a flowchart of calibration of a preliminary reagent in one embodiment of the present invention. As for the reference numerals in the figure, 1 is an automatic chemical analyzer, 2 is a reaction table, 3 is a reaction cuvette, 4 is a reaction line, 5
Is a sample dispensing device, 6 is a sample table, 7 is a sample dispensing unit,
Reference numeral 8 denotes an arrow indicating the direction of movement of the reaction table, 9 denotes a reagent dispensing device, 10 denotes a reagent table, 11 denotes a reagent dispensing unit, 12 denotes a sample dispensing position, 13 denotes a sample dispensing nozzle, and 14 denotes a sample dispensing nozzle. Movement path, 15 is a sample collection position, 16 is a sample container, 17 is a reagent dispensing position, 18 is a reagent dispensing nozzle, 19 is a moving path of the reagent dispensing nozzle, 20 is an arrow indicating a moving direction of the reagent table, 21 is a reagent collection position, 22 is a reagent container, 23 is a moving direction of intermittent driving of the sample table, 24 is a sample container for a sample of a known concentration, and 25 is a container containing a spare reagent.

Claims (2)

(57) [Claims] 1. An automatic chemical analysis method in which a reagent collection is switched from a used reagent container to a spare reagent container containing the reagent and analyzed by dispensing a spare reagent from the spare reagent container. From the measured absorbance, a provisional analysis value is obtained by substituting the analysis value calculation constant of the used reagent that has run out of the reagent as the analysis value calculation constant of the spare reagent used for the analysis, and then, for each of the calibration samples, An automatic chemistry wherein an analytical value calculation constant of the preliminary reagent is obtained from the measured absorbance by dispensing the reagent, and the provisional analytical value is corrected by the analytical value calculation constant of the preliminary reagent. Analysis method. 2. A reaction line is formed, a sample dispensing device, a reagent dispensing device and a measuring device including a used reagent container and a spare reagent container along the reaction line, and an analysis operation for controlling the operation of these devices. A control device is provided to perform reagent dispensing while monitoring the remaining amount of the reagent in the used reagent container at the time of analysis, and when the remaining amount of the reagent reaches a predetermined amount, remove the spare reagent from the spare reagent container. In an automatic chemical analyzer that dispenses into a reaction container disposed in a reaction line, an analysis operation control device monitors a remaining amount of a reagent in each reagent container, and a reagent in the reagent remaining amount management unit. An analysis item determining unit that receives an input signal and determines an analysis item that uses a spare reagent; and receives a signal from the analysis item determination unit and uses the spare reagent as an analysis value calculation constant of the used spare reagent. Use of previous reagent run out Analytical data management unit that continuously uses the analysis value calculation constant of the drug and calculates the analysis value with the correction signal, and dispenses the calibration sample to the reaction vessel for the preliminary reagent and measures the absorbance of the reaction vessel Then, the sample dispensing unit, the reagent dispensing unit and the correction reagent calibration unit that operates the measurement unit to obtain the analysis value calculation constant of the preliminary reagent, and the correction calculation signal of the analysis value calculation constant from the correction reagent calibration unit, An automatic chemical analysis apparatus comprising: an analysis value correction operation unit that corrects an analysis value to which the calculation correction signal is added.