JPH02120056U - - Google Patents
Info
- Publication number
- JPH02120056U JPH02120056U JP2842089U JP2842089U JPH02120056U JP H02120056 U JPH02120056 U JP H02120056U JP 2842089 U JP2842089 U JP 2842089U JP 2842089 U JP2842089 U JP 2842089U JP H02120056 U JPH02120056 U JP H02120056U
- Authority
- JP
- Japan
- Prior art keywords
- excitation
- light
- switching device
- optical fiber
- wavelength switching
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000005284 excitation Effects 0.000 claims description 14
- 239000013307 optical fiber Substances 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims 2
- 230000004907 flux Effects 0.000 claims 1
- 238000010586 diagram Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- YFHXZQPUBCBNIP-UHFFFAOYSA-N fura-2 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=3OC(=CC=3C=2)C=2OC(=CN=2)C(O)=O)N(CC(O)=O)CC(O)=O)=C1 YFHXZQPUBCBNIP-UHFFFAOYSA-N 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
Description
第１図は本考案の１具体例である、高速励起波
長切換装置を備えた励起蛍光強度による画像解析
装置の概念図、第２図は従来の励起波長切換装置
を備えた励起蛍光強度の測定装置の概念図、第３
図は蛍光性カルシウム指示薬Ｆｕｒａ２の遊離カ
ルシウムイオン結合時の蛍光特性曲線のグラフで
ある。
１：光源、２：熱線カツトフイルタ、３：石英
レンズ、４：光フアイバ、５：チヨツパ、６：励
起フイルタ、７：光フアイバ、８：ダイクロイツ
クミラー、９：対物レンズ、１０：サンプル、１
１：画像処理用カメラ、１２：フオトマル、１３
：チヨツパミラー、１４：開口、１５及び１８：
励起フイルタ、１６及び１７：ミラー。
Figure 1 is a conceptual diagram of an image analysis device using excitation fluorescence intensity equipped with a high-speed excitation wavelength switching device, which is a specific example of the present invention, and Figure 2 is a measurement of excitation fluorescence intensity using a conventional excitation wavelength switching device. Conceptual diagram of the device, 3rd
The figure is a graph of the fluorescence characteristic curve of the fluorescent calcium indicator Fura2 when it binds to free calcium ions. 1: Light source, 2: Hot ray cut filter, 3: Quartz lens, 4: Optical fiber, 5: Chipper, 6: Excitation filter, 7: Optical fiber, 8: Dichroic mirror, 9: Objective lens, 10: Sample, 1
1: Image processing camera, 12: Photographer, 13
: Chiyotupa mirror, 14: Opening, 15 and 18:
Excitation filters, 16 and 17: mirrors.
Claims (1)
イルタを介して異なる励起波長のパルス光を得て
、該パルス光を蛍光顕微鏡の励起光路に入射させ
る励起波長切換装置において、光源からチヨツパ
又は励起フイルタへの光路、並びに、励起フイル
タ又はチヨツパから蛍光顕微鏡の励起入射口への
光路を光フアイバ束で構成し、光束の分割並びに
集束を光フアイバ束の分岐並びに集束で行うこと
をことを特徴とする高速励起波長切換装置。 In an excitation wavelength switching device that splits a light beam from a light source, obtains pulsed light of different excitation wavelengths through a chopper and an excitation filter, and enters the pulsed light into the excitation optical path of a fluorescence microscope, from the light source to the chopper or excitation filter. and the optical path from the excitation filter or tipper to the excitation entrance of the fluorescence microscope are composed of optical fiber bundles, and the splitting and focusing of the light flux is performed by splitting and focusing the optical fiber bundles. Excitation wavelength switching device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2842089U JPH02120056U (en) | 1989-03-15 | 1989-03-15 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2842089U JPH02120056U (en) | 1989-03-15 | 1989-03-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH02120056U true JPH02120056U (en) | 1990-09-27 |
Family
ID=31251770
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2842089U Pending JPH02120056U (en) | 1989-03-15 | 1989-03-15 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02120056U (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS585631A (en) * | 1981-07-02 | 1983-01-13 | Masaaki Motoi | Spectrochemical analysis device |
| JPS62192632A (en) * | 1986-02-15 | 1987-08-24 | カ−ル・ツアイス−スチフツング | Microscope photometer |
| JPS62235548A (en) * | 1986-04-07 | 1987-10-15 | Hitachi Ltd | Fluorescence absorption analysis method and apparatus |
| JPS6361143A (en) * | 1986-08-30 | 1988-03-17 | Shimadzu Corp | System for measuring gas concentration distribution |
-
1989
- 1989-03-15 JP JP2842089U patent/JPH02120056U/ja active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS585631A (en) * | 1981-07-02 | 1983-01-13 | Masaaki Motoi | Spectrochemical analysis device |
| JPS62192632A (en) * | 1986-02-15 | 1987-08-24 | カ−ル・ツアイス−スチフツング | Microscope photometer |
| JPS62235548A (en) * | 1986-04-07 | 1987-10-15 | Hitachi Ltd | Fluorescence absorption analysis method and apparatus |
| JPS6361143A (en) * | 1986-08-30 | 1988-03-17 | Shimadzu Corp | System for measuring gas concentration distribution |
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