JPH01502428A - Cosmetic composition and method for producing the same - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] The present invention provides a method for producing biologically active Gantt and metal ion-ligand complexes. and concerning cosmetic preparations containing metal ions or metal ion complexes. .

Disastrous summer limb distortion Various types of cosmetic compositions have been used throughout history.

These cosmetic compositions primarily preserve the elasticity and youthfulness of aging skin. It helps increase it. The first cosmetics were vegetable oils, later emulsified against this agent and water are added to turn into an emulsion (cream), thus creating a cream. Natural oils and fats, such as olive oil, peanut oil, seal oil, etc. The effects of cod liver oil, etc. were enhanced.

Currently, a large number of oils, fats and their derivatives are used in the cosmetic industry. The majority of zero compositions contain so-called "active agents" added to the aqueous or oily phase. Also includes. These active ingredients are added to keep aging skin strong and youthful. be done. Examples of these active ingredients include, for example, vitamins, e.g. Provitamins such as A, B, C, D, E, and F and pantothenic acid, e.g. For example, β-carotene, Special Table 1-502428 (2) Riper complex (Riper-complex, protein, plant extract) and extracts of marine organisms including algae and species. Medications can also be mixed into the cream. In other words, certain medicines are also It exists in the form of a ream. This type of medicine must be administered intradermally. This is particularly desirable when These creams contain anti-inflammatory agents, e.g. Chlorocid-H@　 and Tetra anti-allergic agents, such as prednisolone-J (Predn isolon-JΦ), Locoid (Locoid), hydrocortisone ( HydrocortisonΦ) and Nicoflex (Nicof Sex) @), as well as anti-rheumatic rules, such as violin (Bayolin)” ), Phogosam @) and Arthflor (Arth rofluor■), etc. Place of cream containing medicine In some cases, side effects may occur, most of which are undesirable.

These side effects occur particularly frequently in the case of antirheumatic compositions. Nord ( Knoll), Josef (J6zsef): Jojserkun, Shredder, Ruf Gy5gyszertan, 5chr2i der.

Rufer and 5chn+ie chen): Hupuma Zoo Teishi See Pharmazeutische Chemie.

The functioning of the nervous systems of higher organisms is characterized by electrical phenomena, so-called "action potentials." It is well known that people can be kicked. For example, light, sound, acupuncture When stimulated by laser puncture, etc., Na ° ions and ion inflow and outflow are substantially changed. As a result of a complex process , impulse conduction occurs, during which chemicals such as acetylcholine, adrenaline, etc. , noradrenaline, dopamine and T-aminobutyric acid, etc. also play a substantial role.

According to one theory, stimuli are transmitted from one cell to another without chemical messengers. You can also be However, these processes are extremely complex and Most of them have not yet been elucidated.

The role of metal ions in the human body is a well-understood area. For example, The above Na”, K”, and Ca” in intense conduction.

Although the role of M and Mg2'-ions is well known, many metals Ions are additionally extremely important.

Enzymes are proteins specific to the human body and act as catalysts. its proper Metal ions are also essential for action. Such enzymes include, for example, Taro enzymes, e.g. carbonic anhydrase (Zn), methylmalonyl-CoA - Mutase (Co), xanthine oxidase (Mo), activated by metal ions enzymes such as arginase (Mn) and acylase (Zn), etc. be able to. Certain metals with many valence states participate in redox reactions. (Fe, Cu, 1Ilo, Co, V, Cr, Sn) 6 For example, Friedem M, nibrothiin-metal ・Interafsilane (Protein-Metall Interactio n)　%　Advances in experimental medicine and ・Biology (Advances in Experimental Me) dicine and Biology) JVol, 48, Blenheim Press (Plenum Press) %2-yo4ri, 1974.

The function of metal ion uptake is as follows: In the first step, metal ions are A surface-bound or complex-forming ligand (L) reacts with the metal ion. :M Soil surface M-surface M+L ML Subsequently, an exchange of ligands may occur, in which the metal ion is e.g. can enter biological systems by penetrating cell membranes and/or undergo metal oxidation processes. Degrees may be changed and eventually MLI, or? IL, Tan in the form of reaches the active center of protein. Frusta da Si liva), Williams, R.G.P.

R, J, P,): Di uptic of elements pi biology Cal Systems, Strafture and Bonding (Dinghe Upta) ke of Elements by Biological Systems +5structure and Bonding) 29, 67/1977 In general, metals are in their reduced state, e.g. Fe”°+Mo”*C It is more active in r'' and vO□゛, etc.

From the above, it can be concluded that metal ions are extremely important to living organisms. Ru. The ability of living organisms to take up metal ions depends to a considerable extent on the surrounding conditions and internal conditions. It depends on the state. The most important characteristics in this regard are pH, ionic strength, such as redox potential and chemical environment.

溌y坏■１丞 The present invention provides a method for producing biologically active Gant from plant materials containing histones. provide a method for

Another object of the present invention is to produce products by reacting plant histone hydrolysates with metal ions. It consists in the production of physically active ion-ligand complexes.

Another object of the invention is to provide at least two components, each of which includes at least one metal element. containing metal ion complexes or metal ion complexes, and at least one component has the lowest degree of oxidation (Oxid ation degree), and contains the metal ions or metals of the individual components. is a cosmetic composition characterized by a metal ion complex.

The present invention focuses on optimal pH, ionic strength, redox system position and chemical environment (ligan To provide a method for introducing metal ions into a living body through the skin while providing (d).

The novel body care composition of the present invention has a hydrating effect (hydra-tizati on) not only to restore and soften the skin, but also to improve its electrical and It regenerates a complete living body through a bioactive effect. Therefore , you can get the recovery of the whole body, and the pain caused by the parts of the body Pain is reduced and removed.

The metal ion-binding or Gant used according to the invention preferably has 5 to 6 p)! Can be treated in value, cream and body care group that the manufacture of the product can be easily achieved by using it; was discovered. An ionic strength of 0.3 is sufficient for tapwater ) can be easily maintained when producing the composition. Essence of the invention lies in the formation of redox systems.

According to the present invention, the redox system includes metal ions having various valences. them to form galvanic elements by acting as space electrodes. is formed by separating the metal ions of Molecules used according to the invention Examples of galvanic chains are: 1) galvanic chains without ligands; Chain: Au//5nC1z + 5nC14/FeCl3 + FeC12/ /Iu2) Galvanic chain with ligand: Au//Sn/Lx/C1z +　Sn/Ly/C14/Fe/Lv/C1s +　Fe/Lz/C1z//A Obviously, complete galvanic chains must not be present in the same cream. stomach. This is because discharge brings equilibrium within a very short period of time. This is because it reaches . According to the present invention, one of the electrodes (Au//5n in this example) C1, +5nC1a) must be present in the initial cream, while Other electrodes (Au//FeC1, +FeC1z) are not included in other creams. There must be. During make-up, treat the skin with one cream. and after a few minutes treat with the other cream. Therefore, perhaps the exchange of ligands Redox reactions occur in the lower layers of the skin, including positive activity and certain effects. give.

The galvanic chain electrodes described above contain them in separate units (Cream A, B). separated from each other by

Of course, more than two galvanic chain members can also be created, in which case must create two or more divided units (A, B, C, etc.) . An example of a three-component composition is as follows: The cream obtained according to Example 5 can be used to treat various disorders, e.g. Complaints include myelosclerosis, tennis spasticity, facial neuritis, and blasted fever. Treats wounds, nodding muscle catalepsy, and shoulder and upper arthritis. Tested on shifting patients. By treating said body part twice a day. , the pain of the 55 treated patients disappeared in all cases.

The ligands used in accordance with the invention are produced by hydrolyzing appropriate histones. It can be manufactured using Certain plant histones, such as those of beet J, R, Hafferl, Duplicate See W. Sci. Pharm, 27, 230 (1959). and mistletoe (+m1stletoe) [Belgium Patent No. 646095] Activities such as [see specification] are already known. Separate the extract and subsequently add water By decomposition, polypeptide chains with good complex-forming ability can be obtained. In a preferred embodiment of the method according to the invention, the plant pulp is Acidified to pH 1.0 with a mineral acid and then pressed. obtained in this way The resulting liquid is centrifuged and dried. The crude extract thus obtained is Purify by chromatography. For this purpose, cation exchange resins, For example, IRC-50 (IRC-50”), Dowex 50 Dub Reyu (Dowex 50W''), DEAE cellulose, etc., or adsorption wood Fat, for example, Amberlite AD-2”) and Diaion HP-20” ) etc. can be used. This purified product is formed by organic acids After converting into its acid addition salt, it is hydrolyzed at 120-150°C for 1-10 hours.

The corresponding metal ions are added to the hydrolysis product obtained in this way, and the surrounding environment is form a metal ion-ligand complex under the conditions.

The present invention will be explained in more detail by the following examples, which illustrate the present invention. It does not limit the scope of protection.

H's No Hi.

rainbow Pulp 1 kg of red beet containing 1 histone base ligand, It was then acidified to pol,o using 6N hydrochloric acid. After leaving it for 24 hours, this pallu The grapes were pressed using a grape press. This material was resuspended in 0.251 water. and pressed again. The squeezed liquid obtained in this way with a concentration of about 0.91 was mixed with 4N hydroxy acid. The mixture was made alkaline to pi (11,5) with sodium chloride. It was left in the refrigerator overnight and then centrifuged. The moisture obtained in this way The moisture material was dried at temperatures up to 60-70°C. The 21g thus obtained The crude material was dissolved in pH 6 phosphate buffer and IRC-50' (carboxyl Function) Applicable to a 5×80 cm glass column packed with a cation exchanger.

p) Elute the column with 1B phosphate buffer. Combine both parts containing histones, Evaporated in vacuo and dried over phosphorus pentoxide in vacuo. obtained in this way Add 2.5g of citric acid to 5g of purified protein (histone) Hydrolysis was carried out at 5°C for 1 hour. This hydrolysis product is evaporated in vacuo and and drying in vacuo over phosphorus pentoxide to give 7.4 g of product.

anus Histone Base P No. 1 Mistletoe (whole plant) instead of Gant's IIIP red turnip The method of Example 1 was followed using an object).

■D The crude product obtained from histone base “No. 1 Gantt” was converted into a cation exchanger. The method described in Example 1 was followed with the difference that the purification was carried out on an adsorption resin instead of I obeyed. Add 10 g of fumaric acid to 20 g of crude product and add 100 ad of water melted inside. Subsequently, 30-acetone was added to this solution. This mixture A mixture of water and acetone (40:50) 1? Pretreated XAD-2' It was applied to a resin-filled (7) 5X80 chromatography column. Ace of gradual weight loss The column was eluted with a water-acetone mixture containing 700 ml of water. caused it to emit. this The concentrate was hydrolyzed at 110°C for 5 hours and was ) Drying in air gave 14.5 g of product.

■Soil Production of body care cream without ligand Ingredient "A" (contains metal ions with high oxidation degree): 1 g of potassium tartrate in 800 - of water NiSO4.7H20,0,0 025■NH,,VO3,2,0■Cu5Oa・5HzO110,Ong ZnSO44HtO11,0mg (NHa)6MO1oza4)1.0.1 ．． 5 mg (Co(NHi)h) C1s, 20.0 mg Fez (SO4) :+, 8.0■ Mg504-7HzO11,0■Mn5O,・N20 and 2.5 μ of H, BO2 was dissolved. Adjust the pn of this solution to 5.5 with sodium bicarbonate. 5 and the following ingredients: 90g white petrolatum, 50g cetyl alcohol Le, 35g white oil and 25g Tween-60 ”) at 70°C.The emulsion was mixed with the fat phase consisting of Cooled to temperature.

Component "B" (contains metal ions with low oxidation degree): 50 μm of sake in 800 μm of distilled water Potassium sodium phosphate followed by 33.5■5nC1z・4)1t o and 8.5 cm of gold chloride were dissolved. By adding sodium bicarbonate Adjust the pu of this solution to 5.5 and add the following ingredients: 90g of white petrolatum , 50g cetyl alcohol, 35g white oil and 25g Tween -60 (TWEEN-60") at 70°C. This emulsion was cooled to ambient temperature with stirring.

Component "C" (contains metal ions with low oxidation degree, component molecules AJ and rBJ and redox form a system): Dissolve 50 μm of potassium sodium tartrate in 800 μm of distilled water and add this To the solution was added 0.0025μ of Cr5Oa.7μ20. sodium hydrogen carbonate Adjust the pn of this solution to 5.5 by adding and the following ingredients: 9 0g white petrolatum, 50g cetyl alcohol, 35g white oil and and 25 g of TWEEN-60'' at 70°C. and mixed. The emulsion was cooled to ambient temperature with stirring.

■D Aeon-1 Gant Bowie Component "A" (metal ion with high degree of oxidation); 10 g of the hydrolysis product according to Example 1 was 00- in tap water, then 1.0 μ of N15O*7H20,0 ,0025■ NBavOs, 2.0■CuSO4,+5HzO110,O w ZnSO4・78zO11,0■ (NHa)Jo, Oza・48zO1 1,5■ CCoCNHz>h]C13,20,0■ Fet(SOe)s, 8 ．． 0■ Mg5O*・78zO11,0■MnSO4・H,O and 2.5 2 HJ (h) was added. The pH of the solution thus obtained was adjusted to 5.5. After that, add the following ingredients: 0g of stearyl monoglyceride, 70g of white petrolatum. 82g cetyl alcohol, 30g corn oil, 30g Tween- 60 (THEEN-60”) and 0.2 g of butyl-hydroxytoluene and 7 Mixed at 0°C. The emulsion was cooled to ambient temperature with stirring.

Component "B" (metal ion with low oxidation degree): Log hydrolysis product according to Example 1 to 7 00- dissolved in tap water, 33.5 mg of 5nCJ zz” 48zO and 8.5 μ of gold chloride was added. The pH of the solution thus obtained was adjusted to 5.5. After preparation, add the following ingredients: 0 g of stearyl monoglyceride, 70 g of white wax. Serine, 82g cetyl alcohol, 30g corn oil, 30g Twee TWEEN-60 (purchased TWEEN-60) and 0.2 g of butyl-hydroxytoluene The mixture was mixed at 70°C with a fat phase consisting of: While stirring this emulsion, warm it to ambient temperature. Cooled to .

anus Ion-1 Gantt "Aze 1-Ingredient "A" (high oxidation gold ion): 10 g of the hydrolyzate according to Example 1 was dissolved in tap water at 300 mZ. and added the metal salt shown as component AJ in Example 5 and added sodium bicarbonate. The p)l of the solution was adjusted to 5.5 by addition. 10g carbopol -940 (Carbopol-940”) was swollen in 700af tap water, Combine these two solutions and add 13g of triethanolamine. Therefore, a jelly was prepared.

Component "B" (metal ion with low oxidation degree): 10 g of the hydrolysis product according to Example 1 was 00 - dissolved in tap water and 33.5μ of 5nCIl t・4) 1to and by adding 8.5 μm of gold chloride and adding sodium bicarbonate. The pR of the solution was adjusted to 5.5. IQg Carbobol-940 (Carbo pol-940”) in 700-g tap water and combine these two solutions. And a jelly was prepared by adding 13 g of triethanolamine. .

international search report PCT/HIJ　87100012 One in 8 -6460950S/10/1964 None

Claims (8)

[Claims] 1. In a method for producing biologically active ligands, a histone-containing plant is used. pulping the object, treating the pulp with an aqueous strong mineral acid of pH 1-3 and pressing; The liquid phase is adjusted to a pH of at least 12 and the protein-rich precipitate is separated and cationic purification on an exchanger or adsorption resin, and the purified product in a 1-30% by weight aqueous solution. Converted to its acid addition salt with organic acid and hydrolyzed at 120-150°C for 1-10 hours. A method characterized by: 2. As plant material it is recommended to use beet species such as red turnip or mistletoe. A method according to claim 1, characterized in that: 3. Characterized by using acetic acid, citric acid, tartaric acid, or fumaric acid as the organic acid The method according to claim 1 or 2, wherein: 4. A method for producing a biologically active metal ion-ligand complex, the method comprising: The hydrolyzate is treated with metal salts and up to 10-1 mol/l of metal ions at ambient temperature. A method characterized by reacting at a concentration of 5. Consisting of at least two components, each component containing at least one metal ion or containing metal ion complexes, in which at least one component is gold with the lowest degree of oxidation. the metal ions or metal ion complexes of the individual components are A cosmetic composition characterized by forming nick chains and being mutually non-reactive. 6. The metal ion is a complex formed by a known single complex-forming molecule. 6. Composition according to claim 5, characterized in that it is in the form of: 7. The metal ions are complexes formed by plant histone hydrolyzate ligands. Composition according to claim 5, characterized in that it is in the form of a body. 8. Distributed as a redox electrode in the component containing the last member of the redox chain. Claims 5 to 5, characterized in that they contain subdivided Au. The composition according to any one of items 7 to 7.