JPH0133155B2 - - Google Patents
Info
- Publication number
- JPH0133155B2 JPH0133155B2 JP21613383A JP21613383A JPH0133155B2 JP H0133155 B2 JPH0133155 B2 JP H0133155B2 JP 21613383 A JP21613383 A JP 21613383A JP 21613383 A JP21613383 A JP 21613383A JP H0133155 B2 JPH0133155 B2 JP H0133155B2
- Authority
- JP
- Japan
- Prior art keywords
- inoculation
- coupler
- container
- chamber
- inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000011081 inoculation Methods 0.000 claims description 39
- 241000894006 Bacteria Species 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 description 12
- 238000000034 method Methods 0.000 description 5
- 238000011109 contamination Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【発明の詳細な説明】
〔発明の利用分野〕
本発明は、種菌を発酵槽などに供給（植菌）す
るための植菌装置に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to an inoculation device for supplying (inoculating) seed bacteria to a fermenter or the like.
従来、種菌を発酵槽などに植菌する場合、火炎
植菌方法あるいは注射植菌方法などが採用されて
いる。これらの方法は、植菌の途中において種菌
が周囲の外気に接触するため、外気中の雑菌の混
入するとか、周囲を種菌により汚染するなどの問
題がある。
Conventionally, when inoculating seed bacteria into a fermenter or the like, a flame inoculation method or an injection inoculation method has been adopted. These methods have problems such as contamination with bacteria from the outside air and contamination of the surrounding area by the inoculum because the inoculum comes into contact with the surrounding outside air during inoculation.
本発明の目的は、種菌の槽への植菌を簡単な装
置で、しかも種菌が外気と接触しないように改良
された植菌装置を提供することである。
An object of the present invention is to provide a simple device for inoculating seed bacteria into a tank, and an improved inoculation device that prevents the seed bacteria from coming into contact with the outside air.
本発明は、ダイヤフラムにより種菌を取込む室
と加圧する室とに２分割された植菌容器と、該植
菌容器へ種菌を取込む室側に連結された閉路式の
カツプラと、該植菌容器の加圧する室側にガスを
供給するように連結されたカツプラと、該カツプ
ラと植菌容器の加圧する室との間にガスを調整す
るバルブとを設けたことを特徴とする。
The present invention provides an inoculation container that is divided into two by a diaphragm into a chamber for taking inoculum and a chamber for pressurizing, a closed-circuit coupler connected to the chamber for taking inoculum into the inoculation container, and a chamber for introducing inoculum into the inoculation container. It is characterized by providing a coupler connected to supply gas to the pressurized chamber side of the container, and a valve for adjusting the gas between the coupler and the pressurized chamber of the inoculation container.
以下、本発明を具体的な実施例に基づき詳細に
説明する。第１図は本発明の一実施例を示し、種
菌を槽に植菌する状態を示している。この図にお
いて、１は発酵槽、２は発酵槽に設けられた植菌
口である。３は閉路形のカツプラであり、ここで
は両路閉路形のカツプラが用いられている。４は
植菌容器であり、この内部はダイヤフラム５によ
り仕切られており、２つの室を有する。６は流入
するガス圧または流量を調整するためのバルブで
あり、７はガス供給源からのガス（この例では圧
縮空気）を植菌容器４に供給するためのカツプラ
であり、このカツプラは閉路形でも閉路形のもの
でも良い。８はガス供給ライン、９はガス供給源
である。この図において、植菌装置は３〜７の各
機器で構成されるものである。
Hereinafter, the present invention will be explained in detail based on specific examples. FIG. 1 shows an embodiment of the present invention, and shows a state in which seed bacteria are inoculated into a tank. In this figure, 1 is a fermenter, and 2 is an inoculation port provided in the fermenter. 3 is a closed-circuit type coupler, and here a double-circuit closed-circuit type coupler is used. 4 is an inoculation container, the inside of which is partitioned by a diaphragm 5 and has two chambers. 6 is a valve for adjusting the inflowing gas pressure or flow rate, and 7 is a coupler for supplying gas (compressed air in this example) from a gas supply source to the inoculation container 4, and this coupler is a closed circuit. It may be a shape or a closed circuit shape. 8 is a gas supply line, and 9 is a gas supply source. In this figure, the inoculation device is composed of 3 to 7 devices.
さて、この図をもとに植菌方法を説明する。ま
ず、予めオートクレーブ等で殺菌された植菌容器
４、ダイヤフラム５、カツプラ３の片側、バルブ
６、カツプラ７の片側を、安全キヤビネツトの中
に入れ、カツプラ３を開いて種菌を植菌容器４の
一方の室の中に入れる。次に、種菌の入つた植菌
容器４、ダイヤフラム５、カツプラ３、バルブ
６、カツプラ７が一体となつた植菌装置を槽１に
近づけ、この植菌口２にカツプラ３によつて火炎
中で取付ける。また、カツプラ７をガス供給ライ
ン８に接続する。このときは、バルブ６は閉じた
状態となつている。カツプラ３の両路を開放し、
植菌の準備が完了した後に、バルブ６を開くと、
圧縮空気が植菌容器４の種菌の入つていない方の
室に供給される。これによつて、ダイヤフラム５
が押され、種菌は発酵槽１内に投入される。この
投入の終了後、カツプラ３を閉じ、火炎中でカツ
プラ３を植菌口２より取り外す。また、カツプラ
７も取り外す。これによつて、植菌操作は完了す
る。植菌装置は、その後殺菌され、次の植菌のた
めに保管される。 Now, the inoculation method will be explained based on this diagram. First, put the inoculation container 4, diaphragm 5, one side of the coupler 3, the valve 6, and one side of the coupler 7, which have been sterilized in advance in an autoclave or the like, into a safety cabinet, open the coupler 3, and pour the inoculum into the inoculation container 4. Put it in one of the rooms. Next, the inoculation device which is made up of the inoculation container 4 containing the inoculum, the diaphragm 5, the coupler 3, the valve 6, and the coupler 7 is brought close to the tank 1, and the inoculation port 2 is placed in a flame with the coupler 3. Install it with Further, the coupler 7 is connected to the gas supply line 8. At this time, the valve 6 is in a closed state. Opening both roads of Katsupura 3,
After completing preparations for inoculation, open valve 6.
Compressed air is supplied to the chamber of the inoculation container 4 that does not contain the inoculum. With this, the diaphragm 5
is pressed, and the seed culture is put into the fermenter 1. After this injection is completed, the coupler 3 is closed and removed from the inoculation port 2 under the flame. Also, remove the cutlet placket 7. This completes the inoculation operation. The inoculation device is then sterilized and stored for the next inoculation.
この実施例によれば、植菌操作は完全にクロー
ズされた状態で行なうので種菌を外気に当てるこ
となく、槽内に植菌することができる。また、こ
の装置は、ダイヤフラムにより２つに仕切られた
室を有する植菌容器と、２つのカツプラと、ガス
調整用のバルブとで構成されており、非常に簡単
な構成となつている。しかも、種菌の層への投入
は、バルブを開くことで実施でき、非常に簡単な
操作で植菌作業を実施できる。 According to this embodiment, since the inoculation operation is performed in a completely closed state, the inoculum can be inoculated into the tank without exposing it to the outside air. Furthermore, this device has a very simple structure, consisting of an inoculation container having a chamber partitioned into two by a diaphragm, two couplers, and a gas adjustment valve. Moreover, the inoculum can be added to the layer by opening the valve, and the inoculation process can be carried out with a very simple operation.
以上説明したように本発明によれば、植菌を簡
単な装置で、しかも種菌が外気と接触しないで実
施することができる。したがつて、植菌の際に雑
菌が槽内に混入することも、また周囲を種菌が汚
染することもなくなる。
As explained above, according to the present invention, inoculation can be carried out with a simple device and without the inoculum coming into contact with the outside air. Therefore, during inoculation, bacteria will not get mixed into the tank, and the surrounding area will not be contaminated by the inoculum.
第１図は本発明の一実施例を示す図面である。
１……発酵槽、２……植菌口、３……閉路形の
カツプラ、４……植菌容器、５……ダイヤフラ
ム、６……バルブ、７……カツプラ、８……ガス
供給ライン、９……ガス供給源。
FIG. 1 is a drawing showing an embodiment of the present invention. DESCRIPTION OF SYMBOLS 1...Fermentation tank, 2...Inoculation port, 3...Closed-circuit-type cutlet, 4...Inoculation container, 5...Diaphragm, 6...Valve, 7...Kutupura, 8...Gas supply line, 9...Gas supply source.
Claims (1)
イヤフラムにより種菌を取込む室と加圧する室と
に２分割された植菌容器と、該植菌容器へ種菌を
取込む室側に連結された閉路式のカツプラと、該
植菌容器の加圧する室側にガスを供給するように
連結されたカツプラと、該カツプラと植菌容器の
加圧する室との間にガスを調整するバルブとを設
けたことを特徴とする植菌装置。1. In an inoculation device that supplies seed bacteria into a tank, there is an inoculation container that is divided into two by a diaphragm into a chamber for taking in the seed bacteria and a chamber for pressurizing, and an inoculation container that is connected to the chamber side that takes the seed bacteria into the inoculation container. A closed-circuit coupler, a coupler connected to supply gas to the pressurized chamber side of the inoculation container, and a valve for adjusting gas between the coupler and the pressurized chamber of the inoculation container are provided. A germ inoculation device characterized by:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21613383A JPS60110281A (en) | 1983-11-18 | 1983-11-18 | Device for inoculating microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21613383A JPS60110281A (en) | 1983-11-18 | 1983-11-18 | Device for inoculating microorganism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60110281A JPS60110281A (en) | 1985-06-15 |
| JPH0133155B2 true JPH0133155B2 (en) | 1989-07-12 |
Family
ID=16683777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21613383A Granted JPS60110281A (en) | 1983-11-18 | 1983-11-18 | Device for inoculating microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60110281A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100767569B1 (en) | 2007-04-24 | 2007-10-17 | 김대환 | Fermentor |
-
1983
- 1983-11-18 JP JP21613383A patent/JPS60110281A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60110281A (en) | 1985-06-15 |
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