JPH0724041Y2 - Incubator - Google Patents

Incubator

Info

Publication number
JPH0724041Y2
JPH0724041Y2 JP1989139565U JP13956589U JPH0724041Y2 JP H0724041 Y2 JPH0724041 Y2 JP H0724041Y2 JP 1989139565 U JP1989139565 U JP 1989139565U JP 13956589 U JP13956589 U JP 13956589U JP H0724041 Y2 JPH0724041 Y2 JP H0724041Y2
Authority
JP
Japan
Prior art keywords
pipe
culture
connection
culture chamber
cylinder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP1989139565U
Other languages
Japanese (ja)
Other versions
JPH0379644U (en
Inventor
雅孝 渡辺
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seiwa KK
Original Assignee
Seiwa KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seiwa KK filed Critical Seiwa KK
Priority to JP1989139565U priority Critical patent/JPH0724041Y2/en
Publication of JPH0379644U publication Critical patent/JPH0379644U/ja
Application granted granted Critical
Publication of JPH0724041Y2 publication Critical patent/JPH0724041Y2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Description

【考案の詳細な説明】 [産業上の利用分野] 本考案は、特に植物の組織培養に適する培養装置に関す
る。
DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention relates to a culture device particularly suitable for tissue culture of plants.

[従来の技術] 従来、植物の組織片を植え付けて組織培養を行なう装置
としては、例えば、第5図に示すようなものが知られて
いる。
[Prior Art] Conventionally, as a device for implanting plant tissue pieces and performing tissue culture, for example, a device shown in Fig. 5 is known.

かかる装置100は、上下2段に仕切られた正面開口の箱
形の本体101と、該本体101の正面に配設された開閉扉10
2a,102bと、からなり、該扉102a,102bを閉めることによ
り本体101を密閉できる構造である。また、本体101の上
段部すなわち仕切板103上には、充填した組織支持体104
に植物の葉の切片等の組織片を任意数植え付けてなる箱
状容器105が載置されている。一方、下段106には、所定
量の培養液が貯留されているタンク107と、該タンク107
から培養液を前記箱状容器105内に供給するための供給
管108と、該箱状容器105から排出された培養液を再びタ
ンク107内に回収するための排出管109と、該供給管108
の中途に配設される送水用ポンプ110と、同じく該供給
管108の中途に配設される雑菌除去用フィルタ111と、か
らなる培養液循環装置と、送風ファン112と、該送風フ
ァン112の送風口に装着される除菌フィルタ113とからな
り、上段に雑菌が混入されていない新鮮な空気を送り込
みむための空気循環装置と、が配設されている。また、
天井部には照明装置114が配設されている。
This apparatus 100 is composed of a box-shaped main body 101 with a front opening that is divided into two upper and lower stages, and an opening / closing door 10 arranged in front of the main body 101.
2a and 102b, and has a structure in which the main body 101 can be sealed by closing the doors 102a and 102b. Further, on the upper part of the main body 101, that is, on the partition plate 103, the filled tissue support 104
A box-shaped container 105 in which an arbitrary number of tissue pieces such as plant leaf slices are planted is placed on the. On the other hand, in the lower stage 106, a tank 107 in which a predetermined amount of culture solution is stored and the tank 107
A supply pipe 108 for supplying the culture solution into the box-shaped container 105 from above, a discharge pipe 109 for recovering the culture solution discharged from the box-shaped container 105 into the tank 107 again, and the supply pipe 108
A culture solution circulation device comprising a water supply pump 110 disposed in the middle of the pump, a bacteria removal filter 111 also disposed in the middle of the supply pipe 108, a blower fan 112, and the blower fan 112. A sterilizing filter 113 attached to a blower port, and an air circulation device for blowing in fresh air free of miscellaneous bacteria are provided in the upper stage. Also,
A lighting device 114 is arranged on the ceiling.

[考案が解決しようとする課題] しかるに、上記した従来の装置100には以下のような問
題がある。
[Problems to be Solved by the Invention] However, the conventional device 100 described above has the following problems.

まず、このような装置100で、大量の培養を行なうため
には、組織支持体104を充填した箱状容器105を大型のも
のにすると共に、この大型の容器105を載置するために
培養装置100自体を相当程度大型のものにしなければな
らない。また、培養装置100を一定数のみしか準備して
いない場合には、培養数の増加にともない、装置100自
体の数をさらに増やさざるを得ず、コスト高となる問題
がある。さらに、逆に、培養規模が常時よりも小さい場
合に、それに合わせた装置100を製作するのもコスト上
不利である。その一方、大型の装置100をそのまま用い
るとすると、空調容積が大きすぎるなど、装置100内の
環境制御の点で無駄が生じる。
First, in order to perform a large amount of culture with such a device 100, the box-shaped container 105 filled with the tissue support 104 is made large, and a culture device for mounting the large container 105 is placed. 100 itself must be fairly large. Further, when only a fixed number of the culture devices 100 are prepared, the number of the devices 100 themselves has to be further increased as the number of cultures increases, which causes a problem of high cost. Further, conversely, when the culture scale is smaller than usual, it is also costly to manufacture the device 100 adapted to it. On the other hand, if the large-sized device 100 is used as it is, the air-conditioning volume is too large and wasteful in terms of environmental control inside the device 100.

しかも、かかる装置100は、通常、前記箱状容器105を載
置して培養を行なう前準備として、紫外線や殺菌ガスな
どで装置100内を滅菌して使用されるものである。しか
しながら、培養装置100内の組織支持体104に組織片を植
え付けたり、あるいは組織片を植え付けた箱状容器105
を搬入したりする際には、扉102aの開閉を行なう必要が
ある。また、組織片の成長度に合わせて培養液の成分交
換を行う際にも装置の扉102bを開閉する必要がある。し
たがって、かかる場合に、装置内に雑菌が混入するおそ
れがあると共に、一旦雑菌が混入した場合には、装置内
全体が汚染されてしまうという欠点がある。
Moreover, such a device 100 is usually used by sterilizing the inside of the device 100 with ultraviolet rays, a sterilizing gas or the like as a preparatory step for placing the box-shaped container 105 and culturing. However, a tissue piece is planted in the tissue support 104 in the culture apparatus 100, or a box-shaped container 105 in which the tissue piece is planted.
When carrying in, it is necessary to open and close the door 102a. Also, the door 102b of the device needs to be opened and closed when the components of the culture solution are exchanged according to the growth degree of the tissue piece. Therefore, in such a case, there is a risk that miscellaneous bacteria may be mixed in the device, and once miscellaneous bacteria are mixed, the entire device will be contaminated.

本考案は、上記した問題点を解消し、組織片の培養数の
増減に合わせて培養室を容易に増加又は減少して設置す
ることができると共に、該培養室内に雑菌が混入するこ
とを防止することができ、しかも万一雑菌が混入した場
合にも装置全体が汚染されることなく最小限に食い止め
ることができる培養装置を提供することを目的とする。
INDUSTRIAL APPLICABILITY The present invention solves the above-mentioned problems, and can easily increase or decrease the number of culture chambers to be installed according to the increase or decrease in the number of cultures of tissue pieces, and prevent contamination by contaminants in the culture chambers. It is an object of the present invention to provide a culturing device that can be kept to a minimum and can be kept to a minimum without contaminating the whole device even if contaminants are mixed.

[課題を解決するための手段] 上記目的を達成するため、本考案の培養装置は『少なく
とも温度を制御するための空調ユニット、及び/又は培
養液を供給するための培養液供給ユニット、を有する生
育環境調整装置と、密閉室内に所定量の組織支持体が充
填されてなる培養室と、前記生育環境調整装置と該培養
室とを相互に接続して密閉管路を形成する供給管系と排
出管系とからなり、かつ該供給管系と排出管系のそれぞ
れが、本管と、該本管の任意の位置から分岐されて該培
養室を並列的に増減して設置可能に形成した複数本の枝
管と、からなる輸送管と、輸送管における供給管系と排
出管系のそれぞれの枝管と前記培養室とを接続するため
の第1及び第2の2個一対の接続体からなるプラグであ
って、各接続体が枝管又は培養室に設けられるジョイン
ト管部に嵌合接続される管継手部を有し、かつ、該第1
の接続体が、外筒体と、該第1の接続体の管継手部に連
通している内管体とからなる二重筒構造に形成されてい
ると共に、常態において内筒体を閉成するように付勢さ
れている、先端面に凸起を備えた第1の弁体を有し、第
2の接続体が、第1の接続体の外筒体と内筒体との間隙
に挿入されて両接続体を連結可能な側周壁を有すると共
に、側周壁内方に第1の接続体との連結時に第1の弁体
の先端面を押圧して開弁される、該第2の接続体の管継
手部と連通しているシリンダ部と、シリンダ部内に配設
され常態においてシリンダ部を閉成するように付勢され
ている一方で第1の接続体との連結時には第1の弁体に
設けた凸起に押圧されて開弁する第2の弁体とを有し、
雑菌の混入を防止しつつ輸送管と培養室とを連通させる
ことができる接続プラグと、を有することを特徴とする
培養装置。』である。
[Means for Solving the Problems] In order to achieve the above object, the culture apparatus of the present invention has “at least an air conditioning unit for controlling temperature and / or a culture solution supply unit for supplying a culture solution”. A growth environment adjusting device, a culture chamber in which a sealed chamber is filled with a predetermined amount of a tissue support, and a supply pipe system that connects the growth environment adjusting device and the culture chamber to each other to form a closed pipeline. A discharge pipe system, and each of the supply pipe system and the discharge pipe system is branched from the main pipe and an arbitrary position of the main pipe so that the culture chambers can be installed in parallel to increase or decrease. A transport pipe consisting of a plurality of branch pipes, and a pair of first and second connecting bodies for connecting the respective branch pipes of the supply pipe system and the discharge pipe system in the transport pipe to the culture chamber A plug consisting of a And a pipe joint portion fitted and connected to the joint pipe portion,
Is formed into a double cylinder structure composed of an outer cylinder and an inner cylinder communicating with the pipe joint portion of the first connector, and the inner cylinder is normally closed. Has a first valve body that is urged so as to have a protrusion on the front end surface, and the second connection body is provided in a gap between the outer cylinder body and the inner cylinder body of the first connection body. And a side peripheral wall that can be inserted to connect both connecting bodies, and that is opened by pressing the tip end surface of the first valve body inwardly when connecting with the first connecting body. And a cylinder portion communicating with the pipe joint portion of the connection body of the first connection body, and is urged so as to close the cylinder portion in the cylinder state while being disposed in the cylinder portion. And a second valve body that is opened by being pressed by the protrusion provided on the valve body,
A culture device, comprising: a connection plug capable of connecting a transport tube and a culture chamber while preventing contamination of various bacteria. ].

[作用] かかる構成では、生育環境制御装置の空調ユニットや培
養液供給ユニットから供給される空気や培養液は、まず
供給管の本管をそれぞれ流れ、次に各本管から分岐され
た枝管内に流れていく。そして、それぞれの枝管に接続
された培養室内に、空気や培養液を供給する。
[Operation] In such a configuration, the air and the culture solution supplied from the air conditioning unit and the culture solution supply unit of the growth environment control device first flow through the main pipes of the supply pipe, respectively, and then in the branch pipes branched from the main pipes. Flow to. Then, air or a culture solution is supplied into the culture chamber connected to each branch pipe.

一方、培養室を通過した空気や余剰の培養液は、同じく
培養室に接続された排出管系の枝管を経由して本管に流
入していき、空調ユニットや培養液供給ユニットに回収
され、雑菌が混入することなく培養装置内を循環する。
On the other hand, the air and surplus culture solution that have passed through the culture chamber flow into the main pipe via the branch pipe of the discharge pipe system that is also connected to the culture chamber, and are collected in the air conditioning unit and the culture solution supply unit. , Circulates in the culture device without contamination.

なお、培養室を増減設する場合は、供給管系及び排出管
系の枝管の数を増減設して、それに接続することにより
容易に行なうことができる。
In addition, when the culture chamber is increased or decreased, it can be easily performed by increasing or decreasing the number of branch pipes of the supply pipe system and the discharge pipe system and connecting them.

[実施例] 以下、本考案にかかる培養装置を図面に示した一実施例
に基づきさらに詳細に説明する。
[Embodiment] Hereinafter, a culture apparatus according to the present invention will be described in more detail with reference to an embodiment shown in the drawings.

第1図は本実施例の培養装置1を構成する部材の配設関
係を示した模式図である。
FIG. 1 is a schematic diagram showing an arrangement relationship of members constituting the culturing apparatus 1 of this example.

すなわち、この培養装置1は、空調ユニット2及び培養
液供給ユニット3からなる生育環境制御装置と、培養室
4と、供給管系5及び排出管系6とからなる輸送管と、
から構成される。
That is, the culture device 1 includes a growth environment control device including an air conditioning unit 2 and a culture solution supply unit 3, a culture chamber 4, a transport pipe including a supply pipe system 5 and an exhaust pipe system 6,
Composed of.

空調ユニット2は、温度の他に、湿度調節、炭酸ガス濃
度等も調節可能な空調機器本体2aと、該空調機器本体2a
に対し空気を圧送するためのファン2bと、該空調機器本
体2aの前面送風口に取着され、あるいは任意間隔をおい
て後述する供給管5の管系の途中に配設される除菌フィ
ルタ2cと、から構成される。
The air conditioning unit 2 includes an air conditioning equipment body 2a capable of adjusting humidity, carbon dioxide concentration, etc. in addition to temperature, and the air conditioning equipment body 2a.
To the front of the air conditioning equipment body 2a, or a fan 2b for sending air under pressure, or a sterilization filter disposed at an arbitrary interval in the middle of the pipe system of the supply pipe 5 described later. It consists of 2c and.

培養液供給ユニット3は、培養液貯液タンク3aと、該タ
ンク3aから培養液を吸い上げるポンプ3bと、該ポンプ3b
の流出口近傍に配設される培養液用除菌フィルタ3cと、
から構成される。なお、上記貯液タンク3a内の液温を調
節する液温調節器(図示せず)を設けるようにしてもよ
い。
The culture solution supply unit 3 includes a culture solution storage tank 3a, a pump 3b for sucking the culture solution from the tank 3a, and the pump 3b.
And a culture solution sterilizing filter 3c disposed near the outlet of the
Composed of. A liquid temperature controller (not shown) for adjusting the liquid temperature in the liquid storage tank 3a may be provided.

培養室4は、培地等、植物の組織片を支持する支持体
(図示せず)が充填されてなり、第2図に示すように、
例えば、箱本体41が上面開口の略直方体状に形成され、
かつ該箱本体41の上面41aを塞ぐ蓋部材42が着脱可能に
取付けられている。また、箱本体41の任意の側壁、例え
ば対向する一対の側壁41b,41cの適宜位置には後述する
輸送管と接続されるジョイント管部41d,41e,41f,41gが
突設形成されている。
The culture chamber 4 is filled with a support (not shown) that supports a tissue piece of a plant such as a culture medium, and as shown in FIG.
For example, the box body 41 is formed in a substantially rectangular parallelepiped shape with an upper surface opening,
Moreover, a lid member 42 that closes the upper surface 41a of the box body 41 is detachably attached. Further, joint pipe portions 41d, 41e, 41f, 41g connected to a transport pipe described later are formed at appropriate positions on arbitrary side walls of the box body 41, for example, a pair of opposing side walls 41b, 41c.

蓋部材42の内側周囲には、第2図(b)に示すように、
ゴムなどから形成されるパッキン材43が敷設されてい
る。箱本体41に装着したときに該箱本体41と蓋部材42と
の間に生じる隙間を防ぐためである。この場合、パッキ
ン材43を箱本体41の上端縁に固着する構造としてもよ
い。また、後述する輸送管の適宜位置、例えば排出管系
6の中途に空気取り入れ口7を設け、ここから流入する
空気の圧力により培養室4内部が常に加圧常態となるよ
うに構成すれば、蓋部材42の密閉を保つ構成としなくて
も雑菌混入を防止できる。また、蓋部材42の少なくとも
上面42aは、透明な部材を用いて、任意の位置に配設し
た照明装置(図示せず)からの光を容易に採り入れるこ
とができる構造とすることが好ましい。
Around the inner side of the lid member 42, as shown in FIG.
A packing material 43 made of rubber or the like is laid. This is to prevent a gap generated between the box body 41 and the lid member 42 when mounted on the box body 41. In this case, the packing material 43 may be fixed to the upper edge of the box body 41. Further, if an air intake 7 is provided at an appropriate position of a transportation pipe, which will be described later, for example, in the middle of the exhaust pipe system 6, and the inside of the culture chamber 4 is always in a normal pressure state due to the pressure of the air flowing in from this, Even if the lid member 42 is not configured to keep hermetically sealed, contamination of various bacteria can be prevented. Further, it is preferable that at least the upper surface 42a of the lid member 42 is made of a transparent member so that light from an illumination device (not shown) arranged at an arbitrary position can be easily taken in.

輸送管は、供給管系5と排出管系6とから構成される。
供給管系5は、空調ユニット2の供給口と、培養室4の
流入口に設けられたジョイント管部41dとの間に接続さ
れる空気循環用供給管51と、培養液供給ユニット3の供
給口とジョイント管部41eとの間に接続される培養液循
環用供給管52と、から構成され、それぞれ本管51a,52a
と、該本管51a,52aから分岐された複数本の枝管51b,52b
とを有する構造である。
The transport pipe is composed of a supply pipe system 5 and a discharge pipe system 6.
The supply pipe system 5 is an air circulation supply pipe 51 connected between the supply port of the air conditioning unit 2 and the joint pipe portion 41d provided at the inlet of the culture chamber 4, and the supply of the culture solution supply unit 3. A culture solution circulation supply pipe 52 connected between the mouth and the joint pipe portion 41e, and main pipes 51a and 52a, respectively.
And a plurality of branch pipes 51b, 52b branched from the main pipes 51a, 52a
And a structure having.

排出管系6は、培養室4の流出口側に設けられたジョイ
ント管部41f,41gと、空調ユニット2または培養液供給
ユニット3との間に接続される、空気循環用排出管61と
培養液循環用排出管62とから構成され、上記供給管系5
と同様、それぞれ本管61a,62aと、該本管61a,62aから分
岐された複数本の枝管61b,62bとを有する構造である。
なお、各枝管51b,52b,61b,62bの数は、培養規模に合わ
せてすなわち培養室4の個数に合わせて配管して適宜該
培養室4を並列的に増減できるものである。
The discharge pipe system 6 is connected between the joint pipe portions 41f and 41g provided on the outlet side of the culture chamber 4 and the air conditioning unit 2 or the culture solution supply unit 3, and the discharge pipe 61 for air circulation and the culture. The supply pipe system 5 is composed of a liquid circulation discharge pipe 62.
Similarly, the structure has main pipes 61a and 62a and a plurality of branch pipes 61b and 62b branched from the main pipes 61a and 62a, respectively.
The number of the branch tubes 51b, 52b, 61b, 62b can be adjusted according to the culture scale, that is, the number of the culture chambers 4 so that the culture chambers 4 can be appropriately increased or decreased in parallel.

上記した培養室4の流入口側のジョイント管部41d,41e
及び流出口側のジョイント管部41f,41gと、上記輸送管
の各枝管51b,52b,61b,62bとの各接続は、第3図(a)
に示すように、上記各枝管を耐熱製のゴム等で構成し、
上記各ジョイント管部を該各枝管の中に密接挿入せしめ
ればよい。なお、上記各ジョイント管部を各枝管から離
脱させたときは、第3図(b)に示すように該各枝管の
末端付近をピンチコック8等で圧接密閉する。また、ジ
ョイント管端部には滅菌したキャップ(図示せず)をか
ぶせて閉塞する。
Joint pipe parts 41d, 41e on the inlet side of the culture chamber 4 described above
And the connection between the joint pipe portions 41f, 41g on the outlet side and the branch pipes 51b, 52b, 61b, 62b of the transport pipe are shown in FIG. 3 (a).
As shown in, each branch pipe is made of heat-resistant rubber or the like,
It suffices if the joint pipe portions are closely inserted into the branch pipes. When each of the joint pipes is detached from each branch pipe, the vicinity of the end of each branch pipe is pressed and sealed with a pinch cock 8 or the like as shown in FIG. 3 (b). In addition, a sterilized cap (not shown) is put on the end of the joint pipe to close it.

また、第4図に示すような接続プラグ10を用いて上記各
枝管とジョイント管部とを接続する構成としてもよい。
この接続プラグ10は、底壁12,21に所定径の管継手部13,
22が突出形成された直径の異なる略円筒状の第1及び第
2の接続体11,20からなる。第1の接続体11は、側周壁
が、底壁12から上記管継手部13とは逆方向に突出形成さ
れた外筒体14と、該外筒体14よりも小径にその内方に突
設形成された内筒体15と、からなる二重筒構造である。
Further, it is also possible to use a connection plug 10 as shown in FIG. 4 to connect the branch pipes and the joint pipe portion.
This connection plug 10 has a bottom wall 12, 21 with a pipe joint portion 13 having a predetermined diameter.
The reference numeral 22 is composed of first and second connection bodies 11 and 20 which are formed in a substantially cylindrical shape and have different diameters. The first connecting body 11 has an outer cylindrical body 14 whose side peripheral wall is formed to project from the bottom wall 12 in the direction opposite to the pipe joint portion 13, and has a smaller diameter than the outer cylindrical body 14 and projects inwardly. A double-cylinder structure including an inner cylinder body 15 formed and formed.

また、先端面16aの略中央部に凸起17を突設してなる第
1の弁体16が、常態において前記底壁12との間に介在配
設した圧縮ばね18等の弾性部材により弾発付勢され、か
つ該弁体先端面16aは前記内筒体15における内周段部に
形成した弁座部15aに当接するように配設され、これに
より管継手部13と連通している内筒体15を常態において
閉成している。なお、19は密封シール用Oリングであ
る。
Further, the first valve body 16 having a protrusion 17 projecting substantially in the center of the tip surface 16a is elastically elastically supported by an elastic member such as a compression spring 18 provided between the first valve body 16 and the bottom wall 12. The valve body tip surface 16a is biased and disposed so as to abut the valve seat portion 15a formed on the inner peripheral step portion of the inner cylindrical body 15, and thereby communicates with the pipe joint portion 13. The inner cylindrical body 15 is normally closed. In addition, 19 is an O-ring for hermetic sealing.

一方、第2の接続体20は、その側周壁23が前記第1の接
続体11の外筒体14と内筒体15との間隙に摺動入脱可能な
幅及び長さに形成されると共に、前記第1の接続体11の
内筒体15内部に進入して前記第1の弁体16を押圧するシ
リンダ部24が側周壁23の内方に突設形成されている。ま
た、該シリンダ部24内部には、前記第1の弁体16の先端
凸起17に押圧される第2の弁体25が弾性部材26を介して
弾発付勢されており、これにより管継手部22と連通して
いるシリンダ部24を常態において閉成している。24aは
シリンダ部24先端付近における内周段部に形成した第2
の弁体25用の弁座部であり、21a,27,28は密封シール用
Oリングである。
On the other hand, the side wall 23 of the second connecting body 20 is formed to have a width and a length that can be slidably inserted into and removed from the gap between the outer cylindrical body 14 and the inner cylindrical body 15 of the first connecting body 11. At the same time, a cylinder portion 24 that enters the inside of the inner cylindrical body 15 of the first connection body 11 and presses the first valve body 16 is formed so as to project inside the side peripheral wall 23. Further, inside the cylinder portion 24, a second valve body 25, which is pressed by the tip protrusion 17 of the first valve body 16, is elastically urged via an elastic member 26. The cylinder portion 24 communicating with the joint portion 22 is normally closed. 24a is a second part formed on the inner peripheral step near the tip of the cylinder part 24.
21a, 27 and 28 are O-rings for hermetic sealing.

かかる接続プラグ10は、上記した各接続体11,20の一
方、例えば第1の接続体11の管継手部13には、上記各枝
管51b,52b,61b,62bが嵌合接続され、一方、第2の接続
体20の管継手部22には、培養室4のジョイント管部41d,
41e,41f,41gが嵌合接続される。このとき、図面に示す
ように各管継手部13,22には離脱防止用の突起13a,22aを
設けておくことが好ましい。
In this connection plug 10, the branch pipes 51b, 52b, 61b, 62b are fitted and connected to one of the above-mentioned connecting bodies 11 and 20, for example, the pipe joint portion 13 of the first connecting body 11, , The pipe joint portion 22 of the second connection body 20 has a joint pipe portion 41d of the culture chamber 4,
41e, 41f and 41g are fitted and connected. At this time, as shown in the drawing, it is preferable that the respective pipe joint portions 13 and 22 are provided with protrusions 13a and 22a for preventing separation.

かかる接続プラグ10を用いれば、非使用時、すなわち両
接続体11,20の非連結時においては、各弁体16,25がそれ
ぞれの弁座部15a,24aに当接して内筒体15とシリンダ部2
4を閉成し、結果として、各枝管と培養室4のジョイン
ト管部とを閉塞していることになる。したがって、培養
室4内や輸送管内に雑菌が混入することはない。
If the connection plug 10 is not used, that is, when the two connection bodies 11 and 20 are not connected, the valve bodies 16 and 25 come into contact with the respective valve seat portions 15a and 24a and the inner cylindrical body 15 is formed. Cylinder part 2
4 is closed, and as a result, each branch pipe and the joint pipe portion of the culture chamber 4 are closed. Therefore, bacteria are not mixed in the culture chamber 4 or the transport pipe.

一方、両者を接続する際には、第1の接続体11の外筒体
14と内筒体15との間隙に、第2の接続体20の側周壁23を
嵌入するだけでよい。このようにすると、まず第1の接
続体11の外筒体14と第2の接続体20の側周壁23とが摺り
合うように嵌入される。所定長嵌入すると、第1の弁体
16の先端凸起17が第2の弁体25を押圧する。引き続きさ
らに嵌入すると、第2の接続体20のシリンダ部24の先端
部が、第1の弁体16の先端面16aに当接し、該弁体16を
押圧する。これにより、両弁体16,25が弾性部材18,26に
抗して開弁方向に移動し、各枝管と培養室4とが連通せ
しめられる。
On the other hand, when connecting both, the outer cylindrical body of the first connecting body 11
It suffices to fit the side peripheral wall 23 of the second connecting body 20 into the gap between the inner cylindrical body 15 and the inner cylindrical body 15. In this case, first, the outer cylindrical body 14 of the first connecting body 11 and the side peripheral wall 23 of the second connecting body 20 are slidably fitted into each other. When inserted for a predetermined length, the first valve body
The tip protrusion 17 of 16 presses the second valve body 25. When the fitting is continued, the tip of the cylinder portion 24 of the second connecting body 20 contacts the tip surface 16a of the first valve body 16 and presses the valve body 16. As a result, both valve bodies 16 and 25 move in the valve opening direction against the elastic members 18 and 26, and the respective branch pipes and the culture chamber 4 are made to communicate with each other.

なお、本実施例においては、上記したように両接続体1
1,20の外筒体14,側周壁23が摺動して接続される構造と
したが、密閉管路を形成できるように第1の接続体11の
内管体15に第2の接続体20の側周壁23が密着する構造で
あれば、第1の接続体11の外筒体14と第2の接続体20の
側周壁23との間に隙間があっても構わない。この場合
は、両者の抜止めのために嵌合保持用のばね等、公知の
係止機構等を用いる。
In addition, in the present embodiment, as described above, both connecting members 1
Although the outer cylindrical body 14 of 1 and 20 and the side peripheral wall 23 are slidably connected to each other, the second connecting body is connected to the inner pipe body 15 of the first connecting body 11 so as to form a closed pipeline. As long as the side peripheral wall 23 of 20 is in close contact, there may be a gap between the outer cylindrical body 14 of the first connecting body 11 and the side peripheral wall 23 of the second connecting body 20. In this case, a known locking mechanism or the like such as a spring for fitting and holding is used to prevent the two from coming off.

次に、本実施例にかかる培養装置1の作用を説明する。Next, the operation of the culture device 1 according to the present embodiment will be described.

まず、あらかじめ培養室4及び輸送管内等を紫外線、殺
菌ガスなどで滅菌する。そして、培養室4のジョイント
管部41d,41e,41f,41gと、供給管系5及び排出管系6の
それぞれの枝管51b,52b,61b,62bとを接続する。
First, the culture chamber 4 and the inside of the transport tube are sterilized in advance with ultraviolet rays, sterilizing gas, and the like. Then, the joint pipe portions 41d, 41e, 41f, 41g of the culture chamber 4 are connected to the branch pipes 51b, 52b, 61b, 62b of the supply pipe system 5 and the discharge pipe system 6, respectively.

この場合、上述した第3図に示したような方法で接続す
る場合は、各ジョイント管部と核枝管の末端部付近をア
ルコール等を塗布して燃焼殺菌させてから、両者を接続
することにより、雑菌の混入を防ぐ必要がある。なお、
第4図で示した接続プラグ10を用いて接続する場合に
も、Oリング21aの円内側と内筒体15の端部外周より内
側の露出した面を殺菌して行なう必要があるが、ワンタ
ッチ方式であるので接続作業中に雑菌が混入することが
ほとんどない。
In this case, when connecting by the method shown in FIG. 3 described above, connect each joint pipe part and the vicinity of the end of the nuclear branch pipe with alcohol etc. to burn and sterilize them before connecting them. Therefore, it is necessary to prevent contamination of various bacteria. In addition,
Even when the connection plug 10 shown in FIG. 4 is used for connection, it is necessary to sterilize the exposed surface inside the circle of the O-ring 21a and the outer periphery of the end portion of the inner cylindrical body 15. Since it is a method, almost no bacteria are mixed in during connection work.

而して、この状態において空調ユニット2及び培養液供
給ユニット3から供給される空気及び培養液は、まず、
ファン2bにより圧送されて、除菌フィルタ2cを通過し、
あるいはポンプ3bにより圧送されて除菌フィルタ3cを通
過し、それぞれの供給管5の本管51a,52aを流れ、次に
各本管51a,52aから分岐された枝管51b,52b内に流れてい
く。各枝管51b,52bには、培養室4が上記したように並
列的に接続されているので、同一条件の空気や培養液が
雑菌で汚染されることなく各培養室4内に供給される。
In this state, the air and the culture solution supplied from the air conditioning unit 2 and the culture solution supply unit 3 are
It is pumped by the fan 2b and passes through the sterilization filter 2c,
Alternatively, it is pumped by the pump 3b, passes through the sterilization filter 3c, flows through the main pipes 51a and 52a of the respective supply pipes 5, and then flows into the branch pipes 51b and 52b branched from the main pipes 51a and 52a. Go. Since the culture chambers 4 are connected in parallel to the branch pipes 51b and 52b as described above, the air and the culture solution under the same conditions are supplied into the culture chambers 4 without being contaminated with various bacteria. .

培養室4を通過した空気あるいは余剰の培養液は、上記
した接続プラグ10等によって接続された排出管系6の各
枝管61b,62bを経由して、その本管61a,62aに流入してい
く。したがって、輸送管の中途において雑菌が混入する
ことなく、空調ユニット2や培養液供給ユニット3に回
収され、再び培養室4に供給される。
The air or excess culture solution that has passed through the culture chamber 4 flows into the main pipes 61a, 62a via the branch pipes 61b, 62b of the discharge pipe system 6 connected by the above-mentioned connecting plug 10 and the like. Go. Therefore, bacteria are not mixed in the middle of the transport pipe, and are collected by the air conditioning unit 2 and the culture solution supply unit 3 and supplied again to the culture chamber 4.

なお、培養室4を増設する場合は、供給管系5及び排出
管系6の枝管51b,52b,61b,62bの数を増設すればよく、
容易に行なうことができる。
In addition, when the culture chamber 4 is added, the number of the branch pipes 51b, 52b, 61b, 62b of the supply pipe system 5 and the discharge pipe system 6 may be increased,
It can be done easily.

ここで、上記した実施例では、生育環境制御装置とし
て、空調ユニット2と培養液供給ユニット3とを配設し
た構成としたが、本考案はかかる構成に限定されるもの
ではなく、制御すべき環境条件に応じて、空調ユニット
2のみ、あるいは培養液供給ユニット3のみを配設する
構成としてもよいし、さらに他の環境制御装置を配設す
る構成としてもよい。
Here, in the above-described embodiment, the growth environment control device has the configuration in which the air conditioning unit 2 and the culture solution supply unit 3 are arranged, but the present invention is not limited to this configuration and should be controlled. Depending on the environmental conditions, only the air conditioning unit 2 or only the culture solution supply unit 3 may be provided, or further another environmental control device may be provided.

[考案の効果] 本考案の培養装置によれば、輸送管の枝管の数を増設す
れば容易に培養室の増設を行なうことができる。また、
任意の無菌室に、培養室をジョイント管部から離脱し搬
入して組織片の植え付け作業を行なうことができるの
で、作業中の雑菌混入を防止することができることはも
ちろんのこと、かかる作業され無菌室で行なえば、培養
室の増設作業時においては無菌雰囲気中で行なわなくて
も雑菌が混入することがない。
[Advantage of the Invention] According to the culture device of the present invention, it is possible to easily add a culture chamber by increasing the number of branch pipes of the transport pipe. Also,
Since it is possible to carry out the work of implanting the tissue piece by detaching the culture chamber from the joint tube part and carrying it into an arbitrary sterile room, it is possible to prevent contamination of bacteria during the work, and of course, such a sterile room. If it is carried out in a room, bacteria will not be mixed in even when the culture room is expanded without performing it in a sterile atmosphere.

また、異なる培養液を供給する場合等においても、培養
室の蓋を開放する必要がなく、無菌状態を保持できる。
Further, even when supplying different culture solutions, it is not necessary to open the lid of the culture chamber, and the sterilized state can be maintained.

したがって、従来のように大型の装置を用いて、大規模
の無菌室内で作業する必要がなく、コスト的にも作業能
率の点からも非常に有利である。
Therefore, it is not necessary to work in a large-scale aseptic chamber by using a large-sized device as in the prior art, which is very advantageous in terms of cost and work efficiency.

また、培養室と生育環境調整装置とが密閉管路を形成し
ている輸送管によって接続されていると共に、培養室が
並列的に接続されているので、1つの培養室が汚染され
ても、該培養室のみを取り外して、該培養室に接続され
ている枝管を閉成すればよく、雑菌による汚染を最小限
に食い止めることができる。
Further, since the culture chamber and the growth environment adjusting device are connected by a transport pipe forming a closed pipe line and the culture chambers are connected in parallel, even if one culture chamber is contaminated, It is only necessary to remove the culture chamber and close the branch pipe connected to the culture chamber, so that contamination by various bacteria can be suppressed to a minimum.

さらに、上記した実施例で示した接続プラグを用いれば
培養室の増設作業をきわめて迅速に行なうことができる
ので、接続作業中に雑菌の混入も防ぐことができる。
Furthermore, by using the connection plug shown in the above-mentioned embodiment, the work of adding the culture chamber can be carried out very quickly, so that it is possible to prevent contamination of bacteria during the work of connection.

【図面の簡単な説明】[Brief description of drawings]

第1図は本考案にかかる培養装置の一実施例の配設関係
を示す模式図、第2図(a)は同実施例で用いた培養室
の斜視図、第2図(b)は第2図(a)B−B線断面
図、第3図(a)は枝管とジョイント管部との接続方法
を説明するための斜視図、第3図(b)は枝管からジョ
イント管部を離脱させたときの端部の密閉方法を説明す
るための斜視図、第4図は接続プラグを示す側断面図、
第5図は従来の培養装置を示す斜視図、である。 1……培養装置 2……空調ユニット 3……培養液供給ユニット 4……培養室 5……供給管系 6……排出管系 10……接続プラグ 11……第1の接続体 20……第2の接続体
FIG. 1 is a schematic view showing an arrangement relationship of an embodiment of a culture apparatus according to the present invention, FIG. 2 (a) is a perspective view of a culture chamber used in the same embodiment, and FIG. 2 (b) is a perspective view. 2 (a) is a sectional view taken along the line BB, FIG. 3 (a) is a perspective view for explaining a method of connecting the branch pipe and the joint pipe portion, and FIG. 3 (b) is a branch pipe to the joint pipe portion. FIG. 4 is a perspective view for explaining a method of sealing the end portion when the connector is detached, FIG. 4 is a side sectional view showing a connection plug,
FIG. 5 is a perspective view showing a conventional culture device. 1 ... Incubator 2 ... Air conditioning unit 3 ... Culture solution supply unit 4 ... Culture chamber 5 ... Supply pipe system 6 ... Discharge pipe system 10 ... Connection plug 11 ... First connector 20 ... Second connection

Claims (1)

【実用新案登録請求の範囲】[Scope of utility model registration request] 【請求項1】少なくとも温度を制御するための空調ユニ
ット、及び/又は培養液を供給するための培養液供給ユ
ニット、を有する生育環境調整装置と、 密閉室内に所定量の組織支持体が充填されてなる培養室
と、 前記生育環境調整装置と該培養室とを相互に接続して密
閉管路を形成する供給管系と排出管系とからなり、かつ
該供給管系と排出管系のそれぞれが、本管と、該本管の
任意の位置から分岐されて該培養室を並列的に増減して
設置可能に形成した複数本の枝管と、からなる輸送管
と、 輸送管における供給管系と排出管系のそれぞれの枝管と
前記培養室とを接続するための第1及び第2の2個一対
の接続体からなるプラグであって、各接続体が枝管又は
培養室に設けられるジョイント管部に嵌合接続される管
継手部を有し、かつ、該第1の接続体が、外筒体と、該
第1の接続体の管継手部に連通している内筒体とからな
る二重筒構造に形成されていると共に、常態において内
筒体を閉成するように付勢されている、先端面に凸起を
備えた第1の弁体を有し、第2の接続体が、第1の接続
体の外筒体と内筒体との間隙に挿入されて両接続体を連
結可能な側周壁を有すると共に、側周壁内方に第1の接
続体との連結時に第1の弁体の先端面を押圧して開弁さ
れる、該第2の接続体の管継手部と連通しているシリン
ダ部と、シリンダ部内に配設され常態においてシリンダ
部を閉成するように付勢されている一方で第1の接続体
との連結時には第1の弁体に設けた凸起に、押圧されて
開弁する第2の弁体とを有し、雑菌の混入を防止しつつ
輸送管と培養室とを連通させることができる接続プラグ
と、 を有することを特徴とする培養装置。
1. A growth environment adjusting device having at least an air conditioning unit for controlling temperature, and / or a culture solution supply unit for supplying a culture solution, and a sealed chamber filled with a predetermined amount of a tissue support. And a supply pipe system and a discharge pipe system that form a closed pipe by connecting the growth environment adjusting device and the culture chamber to each other, and each of the supply pipe system and the discharge pipe system. A transport pipe comprising a main pipe and a plurality of branch pipes branched from an arbitrary position of the main pipe so that the culture chambers can be installed in parallel to increase or decrease, and a supply pipe in the transport pipe. A plug comprising a pair of first and second connecting bodies for connecting the respective branch pipes of the system and the discharge pipe system to the culture chamber, each connection body being provided in the branch pipe or the culture chamber. Has a pipe joint portion fitted and connected to the joint pipe portion, and The first connecting body is formed in a double cylinder structure including an outer cylinder body and an inner cylinder body communicating with the pipe joint portion of the first connection body, and the inner cylinder body is normally closed. A first valve body having a protrusion on its tip surface, which is biased to form a second connection body, and the second connection body is a gap between the outer cylinder body and the inner cylinder body of the first connection body. Has a side peripheral wall that can be connected to both connection bodies, and presses the tip end surface of the first valve body when connecting with the first connection body inwardly of the side peripheral wall to open the valve. The cylinder portion communicating with the pipe joint portion of the second connecting body and the cylinder portion disposed in the cylinder portion and being normally biased so as to close the cylinder portion. It has a second valve body that is opened by being pressed upon the protrusion provided on the first valve body, and can prevent the contamination of various bacteria and allow the transport tube and the culture chamber to communicate with each other. Culture apparatus characterized by having a connection plug.
JP1989139565U 1989-12-01 1989-12-01 Incubator Expired - Lifetime JPH0724041Y2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1989139565U JPH0724041Y2 (en) 1989-12-01 1989-12-01 Incubator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1989139565U JPH0724041Y2 (en) 1989-12-01 1989-12-01 Incubator

Publications (2)

Publication Number Publication Date
JPH0379644U JPH0379644U (en) 1991-08-14
JPH0724041Y2 true JPH0724041Y2 (en) 1995-06-05

Family

ID=31686599

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1989139565U Expired - Lifetime JPH0724041Y2 (en) 1989-12-01 1989-12-01 Incubator

Country Status (1)

Country Link
JP (1) JPH0724041Y2 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63240722A (en) * 1987-03-27 1988-10-06 株式会社小松製作所 Seedling raising apparatus

Also Published As

Publication number Publication date
JPH0379644U (en) 1991-08-14

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