JPH01308963A - Suction of red blood cell sample - Google Patents
Suction of red blood cell sampleInfo
- Publication number
- JPH01308963A JPH01308963A JP6522689A JP6522689A JPH01308963A JP H01308963 A JPH01308963 A JP H01308963A JP 6522689 A JP6522689 A JP 6522689A JP 6522689 A JP6522689 A JP 6522689A JP H01308963 A JPH01308963 A JP H01308963A
- Authority
- JP
- Japan
- Prior art keywords
- nozzle
- blood
- blood cell
- clot
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000003743 erythrocyte Anatomy 0.000 title abstract 5
- 210000004369 blood Anatomy 0.000 claims abstract description 19
- 239000008280 blood Substances 0.000 claims abstract description 19
- 210000000601 blood cell Anatomy 0.000 claims description 31
- 208000007536 Thrombosis Diseases 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 14
- 239000003146 anticoagulant agent Substances 0.000 claims description 13
- 229940127219 anticoagulant drug Drugs 0.000 claims description 13
- 210000002966 serum Anatomy 0.000 abstract description 9
- 238000001514 detection method Methods 0.000 abstract description 2
- 238000005070 sampling Methods 0.000 abstract 2
- 230000004520 agglutination Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000009600 syphilis test Methods 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、抗凝固剤を添加せずに血球サンプルをサンプ
ル管内に収容して得られる血餅から、血球サンプルを分
注ノズルによって吸引する血球サンプル吸引方法に関す
るものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a blood cell sample suction method for aspirating a blood cell sample using a dispensing nozzle from a blood clot obtained by storing a blood cell sample in a sample tube without adding an anticoagulant. be.
一般に、採取した血液の採量、希釈、試薬の添加、反応
、測定、測定結果の印字等に至る過程の大部分を自動化
した生化学分析装置には、採取血液からの血球の分取を
容易とするために採取血液に抗凝固剤を添加したサンプ
ルが用いられている。しかし、斯種の分析装置にて例え
ば、血液型の裏検査、抗体スクリーニング検査、梅毒検
査等の検査を行なう場合、これらの検査は抗凝固剤によ
るＰＨ変動等の影響を受は易いため、これらの検査は血
漿よりも抗凝固剤を添加していない血液を遠心分離して
得られる血清により行なう方が精度も向上して望ましい
とされている。そこで前記分析装置には抗凝固剤の添加
されていない血液サンプルも用いられている。このため
に採血時には抗凝固剤入りと、無しとの２本分のサンプ
ルを採取する必要があった。In general, biochemical analyzers that automate most of the processes from blood collection, dilution, addition of reagents, reactions, measurements, and printing of measurement results, make it easy to separate blood cells from collected blood. In order to do this, a sample is used in which an anticoagulant is added to the collected blood. However, when using this type of analyzer to perform tests such as blood type background tests, antibody screening tests, and syphilis tests, these tests are easily affected by pH fluctuations caused by anticoagulants. It is said that it is preferable to perform this test using serum obtained by centrifuging blood to which no anticoagulant has been added, rather than using plasma, as this will improve accuracy. Therefore, blood samples to which no anticoagulant is added are also used in the analyzer. For this reason, when collecting blood, it was necessary to collect two samples, one with anticoagulant and one without.
抗凝固剤を添加していない血液サンプルは、フィブリノ
ーゲンがフィブリンに転換して、血球成分をも取り込ん
で凝固した柔らかい粘着性の血餅と血清との２屑に分離
される。従って血清は従来の生化学分析装置に用いられ
ている分注装置でも、ノズルを単に上下動させて、その
ノズルをサンプル容器内に進入させて簡単に分注するこ
とができるが、このような分注装置により血餅から血球
を分取しようとしても、ノズルが血餅によりつまり易く
、正確な分注ができない。In a blood sample to which no anticoagulant has been added, fibrinogen is converted to fibrin, and it is separated into two pieces: serum and a soft sticky blood clot, which is coagulated by incorporating blood cell components. Therefore, serum can be easily dispensed using a dispensing device used in conventional biochemical analyzers by simply moving the nozzle up and down and entering the nozzle into the sample container. Even if an attempt is made to separate blood cells from a blood clot using a dispensing device, the nozzle is easily clogged with blood clots, making accurate dispensing impossible.
そこで本発明の目的は、抗凝固剤が添加されていない純
血サンプルから得られる血餅から血球成分を分取して、
これを所望な分析系へと分注する血球サンプル吸引方法
を提供することにある。Therefore, the purpose of the present invention is to separate blood cell components from blood clots obtained from pure blood samples to which no anticoagulant has been added.
The object of the present invention is to provide a method for aspirating a blood cell sample to dispense it into a desired analysis system.
なお、本発明は血餅を含んだサンプルでも、血餅のまわ
りにフリーの血球が多数存在することを発見し、その血
球を効果的に吸引して正確に分注するようにすれば、採
取時における採血量を少量とし得ると云う認識に基いて
成したものである。The present invention has discovered that even in samples containing blood clots, there are many free blood cells around the blood clots, and if the blood cells can be effectively aspirated and accurately dispensed, the collection process will be easier. This was done based on the recognition that only a small amount of blood can be collected at a given time.
本発明は、抗凝固剤を添加せずに血液サンプルをサンプ
ル管内に収容して得られる血餅から、血球サンプルを分
注ノズルによって吸引する血球サンプル吸引方法におい
て、分注ノズルの先端部をサンプル管内の内壁面に圧接
させながら、該ノズルをサンプル管の内壁面と血餅との
間に進入させて、血餅とサンプル管の内面との間のフリ
ーな血球サンプルを吸引することを特徴とする血球サン
プル吸引方法にある。The present invention relates to a blood cell sample aspiration method in which a blood cell sample is aspirated using a dispensing nozzle from a blood clot obtained by storing a blood sample in a sample tube without adding an anticoagulant. The nozzle is introduced between the inner wall surface of the sample tube and the blood clot while being in pressure contact with the inner wall surface of the sample tube, and the free blood cell sample between the blood clot and the inner surface of the sample tube is aspirated. There is a blood cell sample aspiration method.
図面につき本発明を説明する。The invention will be explained with reference to the drawings.
第１図は本発明による血球サンプル吸引方法を適用する
装置の一例を示す概略構成図である。FIG. 1 is a schematic configuration diagram showing an example of an apparatus to which a blood cell sample aspiration method according to the present invention is applied.
採取した血液をサンプル管１に入れて抗凝固剤を添加せ
ずに放置または遠心分離機にかけると、下部には血餅２
ができ、上部には血清３が分離される０本発明は斯かる
血清２とサンプル管１の内面との間のフリーの血球を吸
引して分注する装置にあって、分注ノズル４はチューブ
５を介してポンプ系（図示せず）に接続されて血餅２の
まわりの血球サンプルを一定量吸引排出でき、しかも図
示なき洗浄手段にてノズル内外の洗浄を行なえるように
したものである。When the collected blood is placed in sample tube 1 and left without adding anticoagulant or centrifuged, blood clots 2 will appear at the bottom.
The present invention is an apparatus for suctioning and dispensing free blood cells between the serum 2 and the inner surface of the sample tube 1, and the dispensing nozzle 4 is It is connected to a pump system (not shown) through a tube 5 to be able to suction and discharge a certain amount of the blood cell sample around the blood clot 2, and also to clean the inside and outside of the nozzle using a cleaning means (not shown). be.
分注ノズル４はブロック６に固定し、また、このブロッ
ク６は軸７を中心に回転可能に取り付けである。軸７に
は歯車８も取り付け、この歯車の片側面とブロック６の
立上り縁部の側面との間に例えばフェルトのようなスベ
リ材９を配置して、ブロック６を押しバネ１ｏにて図示
のように歯車８に押し当てる。前記軸７はブロック１１
に固定させ、このブロックにはモータ１２をフレーム１
３を介して取り付け、このモータの回転軸に前記歯車８
に噛合する歯車１４を固定する。The dispensing nozzle 4 is fixed to a block 6, and the block 6 is rotatably mounted around an axis 7. A gear 8 is also attached to the shaft 7, and a sliding material 9, such as felt, is placed between one side of the gear and the side of the rising edge of the block 6, and the block 6 is pushed by a spring 1o as shown in the figure. Press it against gear 8 as shown. The shaft 7 is a block 11
The motor 12 is fixed to the frame 1 on this block.
3, and the gear 8 is attached to the rotating shaft of this motor.
A gear 14 that meshes with is fixed.
モータ１２を正逆転させることにより歯車１４を介して
歯車８を回転させ、この回転によりスベリ材９を介して
ブロック６を回動させることによってノズル４を矢印１
５にて示すように弧状に振らせることができる。By rotating the motor 12 in the forward and reverse directions, the gear 8 is rotated via the gear 14, and this rotation rotates the block 6 via the sliding material 9, thereby moving the nozzle 4 in the direction indicated by the arrow 1.
As shown in 5, it can be swung in an arc.
前記ブロック１１はラック１６に固定されており、この
ラックをビニオン１７とモータ１８により動かしてブロ
ック１１を軸ガイド１９に沿って上下動させることによ
り、分注ノズル４をサンプル管１内に出入りさせること
ができる。The block 11 is fixed to a rack 16, and the rack is moved by a pinion 17 and a motor 18 to move the block 11 up and down along an axis guide 19, thereby moving the dispensing nozzle 4 in and out of the sample tube 1. be able to.
本発明による血球サンプル吸引方法を第２ＡおよびＢ図
を参照して説明する。A blood cell sample aspiration method according to the present invention will be described with reference to FIGS. 2A and 2B.
先ずノズル４に隣接して設けられる例えば境界面検知セ
ンサ（図示せず）にてノズル４の上下位置を制御すべく
モータ１８を回転させて、ノズル４を降下させる。この
ノズルの先端部がサンプル管１内に進入した際にモータ
１２を回転させてノズル４の先端部をサンプル管１の内
壁面に第２Ａ図に示すように押し当てる。この状態でノ
ズル４をサンプル管１の内壁面と血餅２との間に降下さ
せ、第２Ｂ図に示す状態でモータ１８を停止させ、図示
なきポンプ系にて血餅２のまわりのフリーの血球Ｐを所
定量吸引し、その吸引完了後再びモータ１８を逆方向に
始動させてノズル４を上昇させる。この際、モータ１２
は回転し続けているも、ノズル４の先端部がサンプル管
１の内壁面に押し当てられるため、このモータ１２はス
ベリ材９の作用により空転状態にあり、ノズル４の先端
部はサンプル管１の内壁面に圧接されたままとなる。First, the nozzle 4 is lowered by rotating the motor 18 to control the vertical position of the nozzle 4 using, for example, a boundary surface detection sensor (not shown) provided adjacent to the nozzle 4 . When the tip of the nozzle enters the sample tube 1, the motor 12 is rotated to press the tip of the nozzle 4 against the inner wall surface of the sample tube 1 as shown in FIG. 2A. In this state, the nozzle 4 is lowered between the inner wall surface of the sample tube 1 and the blood clot 2, the motor 18 is stopped in the state shown in FIG. 2B, and a free pump around the blood clot 2 is removed using a pump system (not shown). A predetermined amount of blood cells P is suctioned, and after the suction is completed, the motor 18 is started in the opposite direction again to raise the nozzle 4. At this time, motor 12
Although the motor 12 continues to rotate, the tip of the nozzle 4 is pressed against the inner wall surface of the sample tube 1, so this motor 12 is idle due to the action of the sliding material 9, and the tip of the nozzle 4 is pressed against the inner wall surface of the sample tube 1. remains in pressure contact with the inner wall surface.
ついでノズル４がサンプル管１がら出る少し前にモータ
１２の回転を反転させてノズル４を元の垂直位置に復帰
させながらノズル４を所定の位置まで上昇させてからモ
ータ１８を停止させると共にノズル４の上昇をも停止さ
せる。つぎに図示なき分析容器に採取した血球サンプル
を排出させると共に、これに図示なきポンプ系にて希釈
液を分注して一定濃度の血球浮遊液を作成し、これをマ
イクロプレートに試薬と共に分注させて所望の分析を行
なうようにする。Then, just before the nozzle 4 exits the sample tube 1, the rotation of the motor 12 is reversed to return the nozzle 4 to its original vertical position while raising the nozzle 4 to a predetermined position, and then the motor 18 is stopped and the nozzle 4 It also stops the rise of Next, the collected blood cell sample is discharged into an analysis container (not shown), and a diluent is dispensed into it using a pump system (not shown) to create a blood cell suspension of a certain concentration, which is then dispensed into a microplate together with reagents. to perform the desired analysis.
なお、本発明は上述した例のみに限定されるものでなく
、例えばノズル４の先端部をサンプル管１の内壁面に押
し当てる手段としては、図示のようなモータ１２を用い
る機構でなく、ソレノイドを用いてノズルを傾斜させる
ようにすることもできる。Note that the present invention is not limited to the above-mentioned example. For example, as a means for pressing the tip of the nozzle 4 against the inner wall surface of the sample tube 1, a solenoid may be used instead of a mechanism using the motor 12 as shown in the figure. It is also possible to tilt the nozzle using
以上、上述した本発明による血球サンプル吸引方法によ
れば、抗凝固剤の入っていない血液サンプルの血餅から
フリーの血球を一定量分取し、これを所望な分析系へと
分注することができ、またこのような吸引方法を用いる
生化学分析装置にて例えば血液型の裏検査、抗体スクリ
ーニング検査、梅毒検査等の検査を行なうに際しても、
同一サンプルの血餅上に分離される上澄血清を用い得る
ため、抗凝固剤によるＰＨ変動等の影響を受けることな
く、純粋な血清により高精度の分析を行なうことができ
る。As described above, according to the blood cell sample aspiration method of the present invention described above, a fixed amount of free blood cells is collected from a blood clot in a blood sample that does not contain an anticoagulant, and this is dispensed into a desired analysis system. Also, when performing tests such as blood type background tests, antibody screening tests, and syphilis tests using biochemical analyzers that use this type of suction method,
Since the supernatant serum separated on the blood clot of the same sample can be used, highly accurate analysis can be performed using pure serum without being affected by pH fluctuations caused by anticoagulants.
さらに本発明による血球サンプル吸引方法によれば、１
本の血液サンプルを用いて血球および血清の双方の生化
学分析を行なうことができるため、採取時における採血
置去なくて済むという利点もある。Furthermore, according to the blood cell sample aspiration method according to the present invention, 1
Since biochemical analysis of both blood cells and serum can be performed using a blood sample, there is also the advantage that there is no need to leave blood at the time of collection.
第１図は本発明による血球サンプル吸引方法を適用する
装置の一例を示す概略図、
第２ＡおよびＢ図は本発明によるサンプル管内の血餅中
にノズルを進入させる前、および進入後のノズルの状態
をそれぞれ示す説明図である。
１−・−・−・・・サンプル管、 ２−・−・−・・・
血餅、３−・・・・・・・・血清、 ４ ・−・
−・・−分注ノズル、５ ・−・−・・・・・チューブ
、 ６ ・・−・・・・・・・ブロック、７ ・・
−・−・・−・軸、 ８−・−・・−・歯車
、９−・・−・−・・−スベリ材、 １０・−・・・
・・−押しバネ、１１・−・・−・・−・ブロック、
１２−・−・−・モータ、１３・・−・・−・・・・
フレーム、１４−・・−・−・歯車、１６−・・・・−
・・−ラック、１７・・・・−・・−ピニオン、１８−
・−・・−・−・モータ、 １９−・−・・軸ガイ
ドＰ ・−・・・・−・・血球。
第１図FIG. 1 is a schematic diagram showing an example of an apparatus to which the blood cell sample aspiration method according to the present invention is applied, and FIGS. 2A and 2B are views of the nozzle before and after the nozzle enters a blood clot in a sample tube according to the present invention. It is an explanatory view showing each state. 1−・−・−・Sample tube, 2−・−・−・・・
Blood clot, 3-...Serum, 4...
-...-Dispensing nozzle, 5...Tube, 6...Block, 7...
−・−・・・・Axle, 8−・−・・・Gear, 9−・・−・−・・−Sliding material, 10・−・・
...-press spring, 11.--.--.-- block,
12−・−・−・Motor, 13・・−・・−・・・
Frame, 14--Gear, 16--
...-Rack, 17--Pinion, 18-
・−・・−・−・Motor, 19−・−・・Axis guide P ・−・・・・−・・Blood cells. Figure 1
Claims (1)
に収容して得られる血餅から、血球サンプルを分注ノズ
ルによって吸引する血球サンプル吸引方法において、分
注ノズルの先端部をサンプル管内の内壁面に圧接させな
がら、該ノズルをサンプル管の内壁面と血餅との間に進
入させて、血餅とサンプル管の内面との間のフリーな血
球サンプルを吸引することを特徴とする血球サンプル吸
引方法。1. In a blood cell sample aspiration method in which a blood cell sample is aspirated using a dispensing nozzle from a blood clot obtained by storing a blood sample in a sample tube without adding an anticoagulant, the tip of the dispensing nozzle is inserted into the sample tube. A blood cell characterized in that the nozzle enters between the inner wall surface of the sample tube and the blood clot while being in pressure contact with the inner wall surface to aspirate the free blood cell sample between the blood clot and the inner surface of the sample tube. Sample aspiration method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6522689A JPH01308963A (en) | 1989-03-17 | 1989-03-17 | Suction of red blood cell sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6522689A JPH01308963A (en) | 1989-03-17 | 1989-03-17 | Suction of red blood cell sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01308963A true JPH01308963A (en) | 1989-12-13 |
| JPH0378582B2 JPH0378582B2 (en) | 1991-12-16 |
Family
ID=13280792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6522689A Granted JPH01308963A (en) | 1989-03-17 | 1989-03-17 | Suction of red blood cell sample |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01308963A (en) |
-
1989
- 1989-03-17 JP JP6522689A patent/JPH01308963A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0378582B2 (en) | 1991-12-16 |
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