JPH0359386B2 - - Google Patents
Info
- Publication number
- JPH0359386B2 JPH0359386B2 JP20914582A JP20914582A JPH0359386B2 JP H0359386 B2 JPH0359386 B2 JP H0359386B2 JP 20914582 A JP20914582 A JP 20914582A JP 20914582 A JP20914582 A JP 20914582A JP H0359386 B2 JPH0359386 B2 JP H0359386B2
- Authority
- JP
- Japan
- Prior art keywords
- blood
- nozzle
- blood clot
- sample
- clot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 208000007536 Thrombosis Diseases 0.000 claims description 32
- 210000000601 blood cell Anatomy 0.000 claims description 20
- 210000004369 blood Anatomy 0.000 claims description 17
- 239000008280 blood Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 14
- 239000003146 anticoagulant agent Substances 0.000 claims description 13
- 229940127219 anticoagulant drug Drugs 0.000 claims description 13
- 238000007599 discharging Methods 0.000 claims description 3
- 238000012360 testing method Methods 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 8
- 238000004140 cleaning Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000009600 syphilis test Methods 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Description
【発明の詳細な説明】
本発明は抗凝固剤を添加せずに血液サンプルを
サンプル管内に収容して得られる血餅から血球サ
ンプルを分注ノズルにより吸入排出する分注方法
に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a dispensing method for inhaling and discharging a blood cell sample from a blood clot obtained by storing a blood sample in a sample tube without adding an anticoagulant through a dispensing nozzle.
一般に、採取した血液の採量、希釈、試薬の添
加、反応、測定、測定結果の印字に至る過程の大
部分を自動化した生化学分析装置には、採取血液
からの血球の分取を容易とするために採取血液に
抗凝固剤を添加したサンプルが用いられている。
しかし、斯種の分析装置にて例えば、血液型の裏
検査、抗体スクリーニング検査、梅毒検査等の検
査を行なう場合、これらの検査は抗凝固剤による
PH変動等の影響を受け易いため、これらの検査は
血漿よりも抗凝固剤を添加していない血液を遠心
分離して得られる血清により行なう方が精度も向
上して望ましいとされている。そこで前記分析装
置には抗凝固剤の添加されていない血液サンプル
も用いられている。このために採血時には抗凝固
剤入りと、無しとの２本分のサンプルを採取する
必要があつた。 In general, biochemical analyzers that automate most of the processes from blood sampling, dilution, addition of reagents, reactions, measurements, and printing of measurement results, make it easy to separate blood cells from collected blood. In order to do this, samples are used in which an anticoagulant is added to the collected blood.
However, when using this type of analyzer to perform tests such as blood type background tests, antibody screening tests, and syphilis tests, these tests are performed using anticoagulants.
Because these tests are susceptible to pH fluctuations, it is preferable to perform these tests using serum obtained by centrifuging blood to which no anticoagulant has been added, rather than using plasma, as this improves accuracy. Therefore, blood samples to which no anticoagulant is added are also used in the analyzer. For this reason, when collecting blood, it was necessary to collect two samples, one with anticoagulant and one without.
抗凝固剤を添加してない血液サンプルは、フイ
ブリノーゲンがフイブリンに転換して、血球成分
をも取り込んで凝固した柔らかい粘着性の血餅と
血清との２層に分離される。従つて血清は従来の
生化学分析装置に用いられている分注方法でも、
ノズルを単に上下動させて、そのノズルをサンプ
ル容器内に進入させて簡単に分注することができ
るが、このような分注方法により血餅から血球を
分取しようとしても、ノズルが血餅によりつまり
易く、正確な分注ができない。 In a blood sample to which no anticoagulant has been added, fibrinogen is converted to fibrin, which incorporates blood cell components and is separated into two layers: a soft, sticky blood clot and serum. Therefore, even with the dispensing method used for conventional biochemical analyzers, serum
Dispensing can be easily done by simply moving the nozzle up and down and entering the sample container, but even if you try to separate blood cells from a blood clot using this dispensing method, the nozzle will not touch the blood clot. This makes it easy to get clogged and prevents accurate dispensing.
そこで本発明の目的は、抗凝固剤が添加されて
いない純血サンプルから得られる血餅から血球サ
ンプルを容易に分取して、これを所望な分析系へ
と分注する方法を提供することにある。 Therefore, an object of the present invention is to provide a method for easily separating a blood cell sample from a blood clot obtained from a pure blood sample to which no anticoagulant has been added and dispensing it into a desired analysis system. be.
なお、本発明は血餅を含んだサンプルでも、血
餅内にフリーの血球が多数存在することを発見
し、その血球を効果的に吸引して正確に分注する
ようにすれば、採取時における採血量を少量とし
得ると云う認識に基いて成したものである。 The present invention has discovered that even in samples containing blood clots, there are many free blood cells inside the blood clots, and if the blood cells can be effectively aspirated and dispensed accurately, the time of collection can be improved. This was done based on the recognition that the amount of blood collected could be kept small.
本発明は抗凝固剤を添加せずに血液サンプルを
サンプル管内に収容して得られる血餅から血球サ
ンプルを分注ノズルにより吸入排出するに当り、
前記ノズルの先端部を血餅中に進入させて停止さ
せ、ついでノズル先端部が血餅から退出を開始し
た後、所定時間経過してから血餅内を抜け出るま
での間にノズルにて血餅中のフリーの血球を吸引
することを特徴とする分注方法にある。 The present invention involves inhaling and discharging a blood cell sample from a blood clot obtained by storing a blood sample in a sample tube without adding an anticoagulant through a dispensing nozzle.
After the tip of the nozzle enters and stops in the blood clot, and then the tip of the nozzle starts to withdraw from the blood clot, the nozzle removes the blood clot after a predetermined period of time has passed and before it exits the blood clot. The dispensing method is characterized by aspirating free blood cells inside.
図面につき本発明を説明する。 The invention will be explained with reference to the drawings.
第１図は本発明による分注方法を実施する装置
の一例を示す線図である。採取した血液をサンプ
ル管１に入れて、抗凝固剤を添加せずに放置また
は遠心分離機にかけると、下部には血餅２がで
き、上部には血清３が分離される。本発明は斯か
る血餅２からフリーの血球を吸引して分注する方
法にあつて、分注ノズル４はレバー５を介してラ
ツク６に固定されており、このラツクをピニオン
７とモータ８によりガイド（図示せず）内にて動
かして、ノズル４を図中矢印Ａにて示すように
上、下方向にのみ可動とする。ノズル４に隣接し
て設けられる例えば境界面検知センサ（図示せ
ず）にてノズル４の上下位置を制御すべくモータ
８を回転させて、ノズルの先端部をサンプル管１
内の血餅２の中に一亘進入させてからモータ８の
回転を停止させてノズル４の降下を停止させる。
この状態でシリンジ９を吸引すると、ノズル４に
は血餅がつまり、また吸引量が少量（例えば数
10μ）では血球はもとより何も吸引することが
できない。 FIG. 1 is a diagram showing an example of an apparatus for implementing the dispensing method according to the present invention. When the collected blood is put into a sample tube 1 and left without adding an anticoagulant or subjected to a centrifuge, a blood clot 2 is formed in the lower part and serum 3 is separated in the upper part. The present invention relates to a method of suctioning and dispensing free blood cells from such a blood clot 2, in which a dispensing nozzle 4 is fixed to a rack 6 via a lever 5, and this rack is connected to a pinion 7 and a motor 8. The nozzle 4 is moved within a guide (not shown) by the arrow A, so that the nozzle 4 can be moved only in the upward and downward directions as shown by arrow A in the figure. For example, a boundary surface detection sensor (not shown) provided adjacent to the nozzle 4 rotates the motor 8 to control the vertical position of the nozzle 4, and the tip of the nozzle is inserted into the sample tube 1.
After entering the blood clot 2 inside the blood clot 2 for a while, the rotation of the motor 8 is stopped and the descent of the nozzle 4 is stopped.
If the syringe 9 is sucked in this state, the nozzle 4 will be clogged with blood clots, and the amount of suction will be small (for example, several
10 μ), it is impossible to aspirate anything, let alone blood cells.
そこで第２図にタイムチヤートにて示すように
ノズル先端部を血餅中に進入させて停止させた時
点t1と同時、またはその時点から多少遅れた時点
t2にノズルを上昇させ、このノズル先端部が血餅
から退出を開始した後の所定時間経過した時点t3
からノズル先端部が血餅を抜け出るまでの間にシ
リンジ９を吸引するようにすれば、第３ａおよび
３ｂ図に示すように、血餅２中に進入させたノズ
ル先端部の抜跡Ｓにはフリーの血球が集まるの
で、シリンジ９の吸引によりチユーブ１０を介し
て斯るフリーの血球を容易に吸引することができ
る。この際、常閉電磁弁１１はシリンジ９とシリ
ンジ１２を連続するチユーブ１３および１４を閉
じており、電磁弁１５はチユーブ１６を介して洗
浄タンク１７内の洗浄液１８でノズル４および各
シリンジへのチユーブ１０，１３，１４等を洗浄
するために洗浄液を通すためのものである。 Therefore, as shown in the time chart in Figure 2, the nozzle tip enters the blood clot and is stopped at the same time as time t1 , or at a time slightly later than that time.
The nozzle is raised at t 2 and a predetermined period of time has elapsed after the nozzle tip begins to withdraw from the blood clot, t 3
If the syringe 9 is sucked between the time when the nozzle tip comes out of the blood clot, as shown in FIGS. Since the free blood cells are collected, such free blood cells can be easily aspirated through the tube 10 by suction with the syringe 9. At this time, the normally closed solenoid valve 11 closes the tubes 13 and 14 that connect the syringe 9 and the syringe 12, and the solenoid valve 15 supplies the cleaning liquid 18 in the cleaning tank 17 to the nozzle 4 and each syringe through the tube 16. This is for passing cleaning liquid to clean the tubes 10, 13, 14, etc.
なお、第２図のタイムチヤートからも明らかな
ように、シリンジ９の吸引動作はノズル４が血餅
２から抜け出る前のt4時点に完了するタイミング
となつている。また、サンプル管１から抜け出た
ノズル４は図示なき他の容器に排出され、これに
一定量の希釈液が分注され、一定の血球浮遊液が
作成され、マイクロプレートに試薬と共に分注さ
せて分析が行われる。 As is clear from the time chart in FIG. 2, the timing is such that the suction operation of the syringe 9 is completed at time t4, before the nozzle 4 comes out of the blood clot 2. Further, the nozzle 4 that has come out of the sample tube 1 is discharged into another container (not shown), into which a certain amount of diluent is dispensed to create a certain blood cell suspension, which is then dispensed into the microplate together with the reagent. Analysis is performed.
上述した例では、分注ノズルを上下動させるこ
とによつてサンプル管中の血餅から血球サンプル
を分注する場合につき述べたが、サンプル管をノ
ズルに対して上下動させることによつても同様に
してサンプル管中の血餅から血球サンプルを分注
し得ることは明らかである。 In the above example, a blood cell sample is dispensed from a blood clot in a sample tube by moving the dispensing nozzle up and down, but it can also be done by moving the sample tube up and down relative to the nozzle. It is clear that a sample of blood cells can be dispensed from a blood clot in a sample tube in a similar manner.
以上、上述した本発明方法によれば、抗凝固剤
の入つていない血液サンプルの血餅からフリーの
血球を一定量分取し、これを所望な分析系へと分
注することができ、またこのような分注方法によ
れば生化学分析装置にて例えば、血液型の裏検
査、抗体スクリーニング検査、梅毒検査等の検査
を行なうに際しても、同一サンプルの血餅上に分
離される上澄血清を用い得るため、抗凝固剤によ
るPH変動等の影響を受けることなく純粋な血清に
より高精度の分析を行なうことができる。 As described above, according to the method of the present invention described above, it is possible to collect a certain amount of free blood cells from the clot of a blood sample containing no anticoagulant, and dispense this into a desired analysis system. Furthermore, according to this dispensing method, even when performing tests such as blood type back test, antibody screening test, syphilis test, etc. using a biochemical analyzer, the supernatant separated on the blood clot of the same sample is Since serum can be used, highly accurate analysis can be performed using pure serum without being affected by PH fluctuations caused by anticoagulants.
さらに本発明による方法によれば、１本の血液
サンプルを用いて血球および血清の双方の生化学
分析を行うことができるため、採取時における採
血量が少なくて済むと云う顕著な利点もある。 Furthermore, the method according to the present invention has the significant advantage that a single blood sample can be used for biochemical analysis of both blood cells and serum, so that only a small amount of blood can be collected at the time of collection.
第１図は本発明による分注方法を実施する装置
の一例を示す線図；第２図は本発明方法の動作説
明用タイムチヤート図；第３ａおよびｂ図はサン
プル管内の血餅中にノズルを進入させた状態およ
び血餅からノズルを抜き取る途中の状態をそれぞ
れ示す説明図である。
１……サンプル管、２……血餅、３……血清、
４……ノズル、５……レバー、６……ラツク、７
……ピニオン、８……モータ、９，１２……シリ
ンジ、１０，１３，１４，１６……チユーブ、１
１，１５……電磁弁、１７……洗浄タンク、１８
……洗浄液。
Fig. 1 is a diagram showing an example of an apparatus for carrying out the dispensing method according to the present invention; Fig. 2 is a time chart diagram for explaining the operation of the method of the present invention; Figs. FIG. 4 is an explanatory diagram showing a state in which the nozzle has entered the blood clot and a state in which the nozzle is being extracted from the blood clot. 1...Sample tube, 2...Blood clot, 3...Serum,
4... Nozzle, 5... Lever, 6... Rack, 7
... Pinion, 8 ... Motor, 9, 12 ... Syringe, 10, 13, 14, 16 ... Tube, 1
1, 15...Solenoid valve, 17...Washing tank, 18
...Cleaning liquid.
Claims (1)
管内に収容して得られる血餅から血球サンプルを
分注ノズルにより吸入排出するに当り、前記ノズ
ルの先端部を血餅中に進入させて停止させ、つい
でノズル先端部が血餅から退出を開始した後、所
定時間経過してから血餅内を抜け出るまでの間に
ノズルにて血餅中のフリーの血球を吸引すること
を特徴とする分注方法。1. When inhaling and discharging a blood cell sample from a blood clot obtained by storing a blood sample in a sample tube without adding an anticoagulant using a dispensing nozzle, enter the tip of the nozzle into the blood clot and stop it. Then, after the nozzle tip starts to exit from the blood clot, free blood cells from the blood clot are sucked by the nozzle after a predetermined period of time has elapsed and before the nozzle exits from the blood clot. Method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20914582A JPS5999262A (en) | 1982-11-29 | 1982-11-29 | Dispensing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20914582A JPS5999262A (en) | 1982-11-29 | 1982-11-29 | Dispensing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5999262A JPS5999262A (en) | 1984-06-07 |
| JPH0359386B2 true JPH0359386B2 (en) | 1991-09-10 |
Family
ID=16568048
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20914582A Granted JPS5999262A (en) | 1982-11-29 | 1982-11-29 | Dispensing method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5999262A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101466788B1 (en) * | 2008-06-17 | 2014-12-01 | 엘지디스플레이 주식회사 | Measuring Device of Rubbing Cloth Thickness and the Measuring Method |
-
1982
- 1982-11-29 JP JP20914582A patent/JPS5999262A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101466788B1 (en) * | 2008-06-17 | 2014-12-01 | 엘지디스플레이 주식회사 | Measuring Device of Rubbing Cloth Thickness and the Measuring Method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5999262A (en) | 1984-06-07 |
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