JPS6365370A - Sample pipetting device - Google Patents

Abstract

PURPOSE:To take a speedy, accurate automatic analysis such as a biochemical analysis by putting a sample sucking, a discharging, and a washing process in simultaneous operation. CONSTITUTION:Pipette nozzles 50 and 51 suck and discharge the sample and are washed with forced water. Further, a holder 54 holds the pipette nozzles 50 and 51 at both end positions, a fitting base 90 supports the holder 54 slidably, and a main shaft 52 supports the fitting base 90. A horizontal driving means composed of a pulse motor 100, etc., moves the holder 54 horizontally, an upward/downward driving means composed of a pulse motor 56, etc., moves up and down the main shaft 52, and a rotary driving means composed of a pulse motor 70, etc., rotates the holder 54 provided at the periphery of the main shaft 52. Then, the sample is sucked and metered by one pipette nozzle and discharged to a reaction pipe, and the other pipette nozzle is washed almost at the same time, so operation for sample dispensation is speeded up greatly and carried out nearly twice as fast as before.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a sample pipette device used in automatic analyzers such as biochemical analysis.

[Prior art] A sample pipette device is used in an automatic analyzer for biochemical analysis, etc., to selectively dispense the required amount of a specimen from a sample container held in a rimple table into a reaction tube on a reaction table. be done.

Here, the specimen is collected from a living body, and the specimen type is divided into general specimens and emergency specimens depending on the urgency of the clinical test. be. The samples are stored in a sample container for general specimens, a sample container for emergency specimens, and a sample container for diluted specimens.

In this manner, in the automatic analyzer described above, a large number of samples are tested, or the sample pipette device is used to dispense samples into reaction tubes.

Conventionally, this type of sample pipette device has already been developed in a patent application filed in 1983.
As described in the specification of No. 0-139553, the pipette nozzle is held at only one end of the holder, and the other end of the holder is supported by a shaft to control the vertical movement and rotation of the shaft as well as the pipette nozzle. 1. The structure is such that a sample can be dispensed into a reaction tube.

FIG. 4 shows the configuration of a conventional sample pipette device. In FIG. 4, the sample pipette device 8 includes an arm (holder) 23 whose one end is pivotally supported by a shaft 22, and a single pipette nozzle 2 held at the thin end of the holder 23.
4, a sampling pump 25 that is communicatively connected to the pipette nozzle 24 and aspirates the required amount of the required sample from the small sample container and discharges it into the corresponding reaction tube, and a sampling pump 25 that moves the holder 23 from the sample suction position to the discharge position into the reaction tube. to the cleaning position via shaft 22
The pipette nozzle 24 is configured to be slidable in the longitudinal direction of the holder 23 in accordance with the measurement item. There is. The sample aspirated from the pipette nozzle 24 fills the suction system flow path with water.
The sample and water are separated by air, and at the time of discharge, only the sample is pushed out by the sampling pump 25 into the reaction tube, and then transferred to the washing position, and the inside of the flow path and pipette nozzle 24 is inside the flow path. It is washed with the remainder of the extrusion water filled in.

The shaft 22 of the sample pipette device 8 is arranged coaxially with the shaft 28 of another reagent pipette device M10.
A detailed explanation of the reagent pipette device 100 will be omitted.

In this way, even with the conventional sample pipetting device described in -F,
A single pipette nozzle can be used to aspirate and eject a sample, wash it with extruded water, and use it in an automatic analyzer.

[Problems to be Solved by the Invention] However, in the above-mentioned conventional lean J. The sequential operation of each step requires a long recycle time, which poses the problem of not being able to perform rapid biochemical analysis or automatic analysis.

The present invention solves these conventional problems.
To provide a Rubipette device that can simultaneously operate the suction and discharge of a specimen and the washing process, and perform automatic analysis such as quick and accurate q-chemical analysis. The purpose is to

[Means for Solving the Problems] In order to achieve the above object, the present invention includes a pair of pipette nozzles for aspirating and discharging a sample and washing with extruded water, and a pipette nozzle for holding the pipette nozzle at both end positions. A holder, a mount for slidably supporting the holder, a main shaft for supporting the mount, a horizontal drive means for horizontally moving the holder, and a horizontal drive means for moving the main shaft up and down. The main shaft is provided with a vertical drive means, and a rotation drive means for avoiding rotation of the holder provided around the main shaft.

[Function] The present invention has the following effects due to the above configuration. In other words, the sample pipette device consists of a sample table in which various sample containers are arranged and housed, a reaction table on the outer periphery of the sample table in which reaction tubes are arranged and housed, and cleaning positions for pipettes 1 to nozzles. It is located almost in the center of the cleaning trough.

By driving the horizontal driving means, the vertical driving means, and the rotational driving means, one pipette nozzle is moved to a required specimen suction position, inserted into the sample container, and the required amount of specimen is aspirated by the sampling pump. At this time, the other pipette nozzle is not operated in any particular way. Next, one pipette 1
- The nozzle is rotated and transferred to the discharge position into the corresponding reaction tube, and the required amount of the specimen is discharged into the reaction tube. At this time, the other pipette nozzle is located at the cleaning trough, and at the same time, the remaining amount of water extruded from the sampling pump flows inside to perform cleaning. Further, the outside of the pipette nozzle is cleaned by jetting and dipping in pure water, and when it is lifted from the cleaning 1-rough, it is dried by blowing compressed air, and the water deposits are removed.

Next, the sample is aspirated by rotating the nozzle to the other pipette 1, and one pipette nozzle is inoperative, and then the sample is ejected into the reaction tube by rotating the other pipette nozzle, and at the same time, one pipette 1~ The nozzle is cleaned. The other pipette nozzle and one pipette nozzle are then rotatably transferred to their respective wash troughs provided in the starting reference position, the pipette nozzle is washed - the cycle is completed, and the pipette nozzle is waited ready for the next cycle. . In this manner, the above-described cycle enables the suction and discharge of the specimen and the washing step to be performed simultaneously, so that automatic analysis such as rapid and accurate biochemical analysis can be performed.

[Embodiment] Hereinafter, the configuration of an embodiment of the present invention will be described in detail with reference to the drawings.

1 to 3 show the structure of an embodiment of the present invention.

In FIG. 1, 54 indicates a holder, and C- in FIG.
As shown in FIG. 2, which is a plan view in the direction of arrow C, and FIG. 3, which is a side view in the direction of arrow C in FIG. It is held almost vertically. The pipette nozzles 50, 51 are connected at their upper ends to a Diko J (not shown), and are further connected to a sampling pump via a solenoid valve. Then, pour water into the microsyringe inside the numbering pump! In
Of course, suction measurement is performed with the sample and water separated by air. Also, pipette nozzle 50°5
1 is provided with a liquid level detection electrode that can detect the liquid level of the specimen in the sample container.

Reference numeral 90 denotes a mounting base, which supports the holder 54 so as to be slidable in the horizontal direction. The horizontal drive means for horizontally moving the holder 54 consists of drive transmission elements such as a pulse motor 100, and in FIGS. 104 right Zuruho I) Shellfish! ) is transmitted to the horizontal movement of 4. Guide roller 92.92° 92, 92 is the mounting base 9
0 and located on both sides of the holder 54 to ensure smooth horizontal movement of the holder 54.

In FIG. 1, reference numeral 52 indicates a main shaft, and a mounting base 90 is supported at the top of the =1 shaft.

The main shaft 52 can move up and down, and as the main shaft 52 moves up and down, the mounting base 90. Holder 54 and pipette nozzle 50.51 are moved up and down. The mounting base 90 is provided with a bush 80.80, and the bush 80.80 is equipped with a branch 7.
8.78 is penetrated, and the struts 78.78 are respectively provided on the upper lever 77 of the member 76 provided around the ↑ shaft 52, and when the main shaft 52 moves up and down, the member 76°, the upper lever 77 and the strut 78.78 is fixed,
The mounting base 90 is moved up and down via the bushings 80.80,
Furthermore, by sliding on the surfaces of the columns 78, 78, a balanced vertical movement is achieved, and as well as the vertical movement of the holder 54, the force for the rotational movement is transmitted.

The vertical drive means for moving the main shaft 52 up and down consists of drive transmission elements including a pulse motor 56, and the pulsed rotation of the pulse motor 56 is caused by gear trains 58, 60, 62.
．． Main shaft 5 with rack 68 from gear 66 via 64
It is transmitted to the vertical movement of 2. Further, the rotational drive means provided around the main shaft 52 to rotate the holder 54 and the mounting base 90 is composed of drive transmission elements such as a pulse motor 70, and the pulsed rotation of the pulse motor 70 is controlled by the gears 72, 74. Rotate the part 447B through the holding tool 5
4 and the handle (J) are transmitted to rotate the table 90.

The automatic analyzer is activated and the rimple pipette device is activated. When the lean-pull table is rotated and a predetermined non-pull container reaches a predetermined sample suction position, the pipette nozzle 50 is driven by the horizontal drive means, vertical drive means, and rotary drive means of the sample pipette 1 to the sample pipette 1. It is inserted into the sample container at suction (i'/Fj).At this time, the liquid level of the sample is detected by the liquid level detection electrode, the downward movement of the pipette nozzle 50 is stopped, and the required amount of sample is collected by the action of the sampling pump'. The pipette nozzle 50 is pulled out from the rippling container after the epigastric movement is carried out, is horizontally moved and rotated to the sample discharge position, is inserted into the reaction tube at that position, and is inserted into the sampling pump. The sample previously aspirated by the extruder is dispensed into the reaction tube.When the dispensing into the reaction tube is completed, the solenoid valve is operated to change the flow path, and the other pipette 1 to nozzle is 51, the remaining amount of extruded water from the sampling pump is pushed out to clean the inner surface. At this time, the pipette nozzle 51 is located at the cleaning position where cleaning 1 to rough are provided, and in the cleaning 1 to rough, the pipette nozzle 51 The specimen adhering to the inner and outer surfaces of the tube is washed with a cleaning solution, and a drying operation is added to the pipette 1 to be used in the next cycle as a clean nozzle 51.

That is, in the next cycle, the pipette nozzle 51 is used to aspirate and extrude the specimen, and also performs the pipette nozzle 51 to aspirate and extract the specimen.
The nozzle 50 is used for a cleaning operation, and then the operation is repeated to return to the next cycle for automatic analysis.

In this way, aspirate the sample with one pipette nozzle.
tffi (as well as discharging into the reaction tube,
Since the other pipette nozzle is washed at the same time, the operation for dispensing the sample can be made extremely quick, and can operate at approximately twice the speed compared to conventional pipettes.

In addition, the specimens are stored in a sample container for general specimens, a sample container for emergency specimens (!3), and a sample container for diluted specimens according to their type, and are held on a sample table (A3). The holder with the pipette nozzle is moved to aspirate the required specimen at different specimen aspiration positions selected according to the test procedure.

In addition, the sample pipette device automatically discharges the sample to be dispensed into the reaction tube into the electrolyte sampling pot installed at the position of the moving rod of the sample pipette device in accordance with the measuring point 1. Measurements can be taken.

[Effects of the Invention] As is clear from the above embodiments, the present invention can quickly perform operations for dispensing a sample and washing operations, and in particular, can perform operations for selecting different types of samples. Compared to conventional sample pipetting devices, the rapidity and analytical ability can be significantly improved, and the device can be made more compact. It has excellent effects.

[Brief explanation of the drawing]

FIG. 1 is a front view of a sample pipette device according to an embodiment of the present invention, FIG. 2 is a view of the same device taken along line C-C in FIG. 1, and FIG. 3 is a view taken along arrow D in FIG. The perspective view, FIG. 4, is an explanatory diagram of a conventional sample pipette device. 50, 51...Pipette nozzle 52...Main shaft 54...Holder 90...Mounting base Patent applicant Nihon Tektron Co., Ltd. Fig. 2 U Fig. 3 εX]...Jβζ I 9

Claims (1)

[Claims] A pair of pipette nozzles for aspirating and discharging a sample and washing with extruded water, a holder for holding the pipette nozzle at both end positions, a mounting base for slidably supporting the holder, and a mounting base. A main shaft for supporting the main shaft, a horizontal drive means for horizontally moving the holder, a vertical drive means for moving the main shaft up and down, and a rotational drive for rotating the holder provided around the main shaft. A sample pipetting device characterized by comprising means.