JPH01291784A - Cultured product stirring method and culture apparatus - Google Patents
Cultured product stirring method and culture apparatusInfo
- Publication number
- JPH01291784A JPH01291784A JP12146588A JP12146588A JPH01291784A JP H01291784 A JPH01291784 A JP H01291784A JP 12146588 A JP12146588 A JP 12146588A JP 12146588 A JP12146588 A JP 12146588A JP H01291784 A JPH01291784 A JP H01291784A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- culture tank
- tank
- inflow path
- angle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003756 stirring Methods 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims description 13
- 241000195493 Cryptophyta Species 0.000 description 13
- 230000005484 gravity Effects 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 230000005587 bubbling Effects 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 235000016425 Arthrospira platensis Nutrition 0.000 description 3
- 240000002900 Arthrospira platensis Species 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 229940082787 spirulina Drugs 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
この発明は、藻類や微生物を培養する際の培地の攪拌方
法及びそれを実施する培養装置に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method for stirring a medium when culturing algae or microorganisms, and a culturing apparatus for carrying out the method.
［従来の技術］
従来から、藻類等を培養する際の培養槽内の培養物の撹
拌方法としては、撹拌板を用いたものが最もよく用いら
れている。しかしながら、撹拌板が培養槽内に存在する
と、光を藻体に当てる際に邪魔になるので、培養槽内に
は構造物が何も存在しないことが好ましい。[Prior Art] Conventionally, the most commonly used method for stirring a culture in a culture tank when culturing algae, etc. is to use a stirring plate. However, if the stirring plate exists in the culture tank, it will get in the way when light is applied to the algae, so it is preferable that no structure exists in the culture tank.
また、二酸化炭素をバブリングさせて藻類に二酸化炭素
を供給すると同時に培養槽内の培地を攪拌するシステム
も使用されている。しかしながら、二酸化炭素をバブリ
ングさせる方法は、無重力下では採用することが不可能
である。すなわち、スペースシャトルや宇宙ステーショ
ンにおいて乗員の食料となる藻体と酸素とを確保するた
めに食用に供することができる例えばスピルリナ等の藻
類を無重力下で培養することが求められているが、この
ような場合には藻類の培養は無重力下で行なわれるので
二酸化炭素のバブリングによって培養物を撹拌すること
はできない。Additionally, a system is also used that supplies carbon dioxide to algae by bubbling it and at the same time stirs the culture medium in the culture tank. However, the method of bubbling carbon dioxide cannot be adopted under zero gravity. In other words, in order to secure algae and oxygen that are food for the crew on the space shuttle and space station, it is required to cultivate edible algae, such as spirulina, in zero gravity. In such cases, algae cultivation is carried out in zero gravity, and the culture cannot be agitated by bubbling carbon dioxide.
［発明が解決しようとする問題点］
従って、この発明の目的は、培養槽内に撹拌板のような
構造物を何ら設けることなく、無重力下においても培養
物の攪拌が可能になる培養物の撹拌方法及びそれを実施
する装置を提供することである。[Problems to be Solved by the Invention] Therefore, an object of the present invention is to provide a culture that enables stirring of the culture even under zero gravity without providing any structure such as a stirring plate in the culture tank. An object of the present invention is to provide a stirring method and an apparatus for carrying out the stirring method.
［問題点を解決するための手段］
すなわち、この発明は、培養槽の下部に培養物流入路を
前記培養槽の縦軸に対して角度を持たせて設け、該培養
物流入路から培養物を培養槽内に流入させ、それによっ
て培養槽内の培養物を渦状に撹拌させることを含む培養
物撹拌方法を提供する。[Means for Solving the Problems] That is, the present invention provides a culture inflow path at the bottom of a culture tank at an angle with respect to the vertical axis of the culture tank, and allows the culture to flow from the culture inflow path. Provided is a method for stirring a culture, which includes causing a culture medium to flow into a culture tank, thereby swirling the culture in the culture tank.
さらにまた、この発明は、培養槽と、該培養槽の下部に
該培養槽の縦軸に対して角度を持たせて設けられた培養
物流入路と、該培養物流入路から前記培養槽内に培養物
を流入させる手段とを含む培養装置を提供する。Furthermore, the present invention also provides a culture tank, a culture inflow path provided in a lower part of the culture tank at an angle with respect to the vertical axis of the culture tank, and a flow path from the culture inflow path into the culture tank. and a means for flowing a culture into the culture device.
［発明の効果］
この発明によると、培養槽内に撹拌板のような構造物を
何ら設けることなく、また、無重力下においても培養槽
内の培養物を撹拌することができる。従って、この発明
の装置を用いて藻類を培養すると、培養槽内の構造物に
よって藻類への光の供給が妨げられることがないので藻
類の生長がより促進される。また、従来の二酸化炭素の
バブリングによる撹拌とは異なり、無重力下においても
培養物の攪拌が可能になる。従って、スペースシャトル
や宇宙ステーション等において乗員の食料及び酸素を得
るために藻類を培養するための装置として非常に有効で
ある。[Effects of the Invention] According to the present invention, the culture in the culture tank can be stirred even under zero gravity without providing any structure such as a stirring plate in the culture tank. Therefore, when algae are cultivated using the apparatus of the present invention, the growth of algae is further promoted since the supply of light to the algae is not obstructed by the structures in the culture tank. Furthermore, unlike the conventional agitation by bubbling carbon dioxide, the culture can be agitated even in zero gravity. Therefore, it is very effective as a device for cultivating algae to obtain food and oxygen for crew members on space shuttles, space stations, etc.
［発明の詳細な説明］
この発明の培養装置は、培養槽と、培養槽の下部に該培
養槽の縦軸に対して角度を持たせて設けられた培養物流
入路を有する。上記角度は、縦、横両方向共に１０度な
いし５０度であることが好ましい、また、培養物流入路
は複数個存在することが培養物のより均一でスムーズな
撹拌のために好ましい。また、この発明の装置は、例え
ばポンプのような、上記培養物流入路から上記培養槽内
に培養物を流入させるための手段を有する。また、培養
槽内における培養物の沈着を防ぐ目的で、培養槽底部中
央に培養物流入路を上記流入路とは別個に設けても一向
差し支えない。[Detailed Description of the Invention] The culture apparatus of the present invention includes a culture tank and a culture inflow path provided at the bottom of the culture tank at an angle with respect to the vertical axis of the culture tank. The angle is preferably 10 degrees to 50 degrees in both the vertical and horizontal directions, and it is preferable to have a plurality of culture inflow channels for more uniform and smooth stirring of the culture. The device of the invention also includes means, such as a pump, for flowing the culture into the culture tank from the culture inflow path. Further, in order to prevent the culture from settling in the culture tank, a culture inflow channel may be provided separately from the above-mentioned inflow channel at the center of the bottom of the culture tank.
この発明の好ましい態様では、上記培養槽は密閉型の培
養槽であり、培養槽の外側を通って培養槽の上部から下
部に至るバイパスラインが通っており、該バイパスライ
ンが培養槽の下部に至る部分が前記流入路となっており
、また、培養物流入路から上記培養槽内に培養物を流入
させるための手段は上記バイパスラインに挿入されたポ
ンプである。バイパスラインには所望により、ｐｌ（メ
ーター、圧力計その他の計器類や、藻体の回収手段や培
地の供給手段を設けることができる。In a preferred embodiment of the invention, the culture tank is a closed type culture tank, and a bypass line passes through the outside of the culture tank from the top to the bottom of the culture tank, and the bypass line is connected to the bottom of the culture tank. The part leading thereto serves as the inflow path, and the means for flowing the culture into the culture tank from the culture inflow path is a pump inserted into the bypass line. If desired, the bypass line can be provided with a pl (meter, pressure gauge, and other instruments), algae collection means, and culture medium supply means.
この発明の方法では、上記培養物を流入させるための手
段を用いて培養物流入路から培養槽内に培養物を流入さ
せる。培養物流入路は培養槽の縦軸に角度を持たせて設
けられているので、培養物の流入によって培養槽内の培
養物が渦状にスムーズに攪拌される。In the method of the present invention, the culture is caused to flow into the culture tank from the culture inflow path using the means for flowing the culture into the culture tank. Since the culture inflow path is provided at an angle to the vertical axis of the culture tank, the culture in the culture tank is smoothly stirred in a vortex by the inflow of the culture.
この発明の装置は上記したようなバイパスラインを有す
る閉鎖系のものが好ましいが、必ずしもそれに限定され
るものではなく、開放系の培養装置であってもよく、ま
た、バイパスラインを設けずに培養槽上部から培養物を
オーバーフローさせるような形態のものであってもよい
、また、この発明の方法は、特に無重力下において威力
を発揮するが、必ずしも無重力下において行なう必要は
なく、重力下においても行なうことができる。Although the device of the present invention is preferably a closed system having a bypass line as described above, it is not necessarily limited to this, and may be an open system culture device, or a culture device without a bypass line may be used. The method of the present invention may be of a form in which the culture overflows from the top of the tank.Although the method of this invention is particularly effective under zero gravity, it is not necessarily necessary to perform under zero gravity, and it can also be performed under gravity. can be done.
さらに、この発明の方法及び装置を用いて培養される生
物は必ずしも藻類に限定されるものではな（、細菌その
他の微生物の培養にも用いることができる。Furthermore, the organisms cultured using the method and apparatus of the present invention are not necessarily limited to algae (although they can also be used to culture bacteria and other microorganisms).
［実施例］
以下、この発明を実施例に基づいてより詳細に説明する
。もっとも、この発明は以下に記載する実施例の他にも
種々の態様が可能であることは明らかであり、以下の実
施例に限定されるものではない。[Examples] Hereinafter, the present invention will be described in more detail based on Examples. However, it is clear that this invention can be modified in various ways in addition to the embodiments described below, and is not limited to the following embodiments.
この発明の培養装置の好ましいｌ実施例の模式図が図１
に示されている６図１において、培養槽ｌＯは密閉型の
ものであり、その上部からバイパスライン１２が延び、
該バイパスライン１２はポンプ１４を介して培養槽１０
の下部に至り、閉鎖培養系が構成されている。バイパス
ラインが培養槽ｌＯの下部に戻る部分は３つに分岐して
流入路１６となっている。培養槽１０の容量は例えば６
１２程度であり、バイパスライン１２の直径は例えばｌ
ｃｗ程度、また、ポンプ１４の能力は例えば５２／分以
下である。A schematic diagram of a preferred embodiment of the culture apparatus of this invention is shown in FIG.
6 In FIG. 1, the culture tank IO is of a closed type, and a bypass line 12 extends from the top thereof.
The bypass line 12 is connected to the culture tank 10 via a pump 14.
A closed culture system is constructed. The portion where the bypass line returns to the lower part of the culture tank IO is branched into three parts to form an inflow path 16. The capacity of the culture tank 10 is, for example, 6
12, and the diameter of the bypass line 12 is, for example, l.
cw, and the capacity of the pump 14 is, for example, 52/min or less.
培養槽１０の縦断面図が図２に示され、培養槽１０の下
部を上から見た図が図３に示されている０図２に示され
るように、培養物流入路１６は、培養槽ｌＯの縦軸１８
に対して角度θを持たτせて設けられている。また、こ
の角度は、図３に明瞭に示されるように、上から見た場
合に培養槽の縦軸に対して縦横両方向に形成されている
。さらに、図３に示されるよう゛に、３つの流入路１６
は正三角形の頂点にそれぞれ位置するように配置され、
培養槽の縦軸に対して互いにほぼ等しい角度で傾斜して
いる。A vertical cross-sectional view of the culture tank 10 is shown in FIG. 2, and a top view of the bottom of the culture tank 10 is shown in FIG. 3.As shown in FIG. Vertical axis 18 of tank lO
It is provided at an angle θ with respect to the angle θ. Moreover, as clearly shown in FIG. 3, this angle is formed both vertically and horizontally with respect to the longitudinal axis of the culture tank when viewed from above. Furthermore, as shown in FIG.
are placed at the vertices of an equilateral triangle,
They are inclined at approximately equal angles to each other with respect to the longitudinal axis of the culture tank.
培養する際には、前記ポンプ１４を作動させて前記培養
槽ｌＯ及びバイパスライン１２に培養物を矢印Ａの方向
に流通させる。そうすると、培養物は流入路１６に到達
した時点で３つに分割され、それぞれの流入路１６から
培養槽１０内に流入する。３つの流入路はそれぞれ培養
ｐａｌＯの縦軸に対して角度を持たせて設けられている
ので、培養槽１０内の培養物は、流入路１６からの培養
物の流入によって渦状にスムーズに撹拌され、所期の目
的が達成される。When culturing, the pump 14 is operated to cause the culture to flow through the culture tank IO and the bypass line 12 in the direction of arrow A. Then, upon reaching the inflow path 16, the culture is divided into three parts and flows into the culture tank 10 from each inflow path 16. Since each of the three inflow channels is provided at an angle with respect to the vertical axis of the culture palO, the culture in the culture tank 10 is smoothly stirred in a vortex by the inflow of the culture from the inflow channel 16. , the intended purpose is achieved.
上記したこの発明の培養装置を試作した。培養槽は直径
１７ｃ＋ｓ、円筒状部分の高さが２５ｃｍで内容量は約
６２であった。また、バイパスラインの直径は１ｃ＋＋
であり、培養物の循環速度は２Ｉ２／分であった。この
装置を用いてスピルリナ・マキシアの撹拌を行なったと
ころ、培養槽内で培養液は下から上へよどみなくらせん
状に回転しながら撹拌され、スピルリナは均一に保たれ
た。The above-mentioned culture device of the present invention was prototyped. The culture tank had a diameter of 17 cm+s, a height of the cylindrical part of 25 cm, and an internal capacity of about 62 cm. Also, the diameter of the bypass line is 1c++
and the circulation rate of the culture was 2I2/min. When Spirulina Maxia was stirred using this device, the culture solution was stirred in a spiral pattern from bottom to top without stagnation in the culture tank, and the Spirulina was kept uniform.
° 図１はこの発明の培養装置の１実施例を模式％式
％
図２はこの発明の培養装置の１実施例の縦断面図、
図３はこの発明の培養装置に採用される培養槽の内部下
部を上から見た図である。
１０・・・培養槽、１２・・・バイパスライン、１４・
・・ポンプ、１６・・・流入管、１８・・・培養槽の縦
軸
引
゛）６
図 ２
図 ３Figure 1 is a schematic representation of an embodiment of the culture device of the present invention. Figure 2 is a vertical sectional view of an embodiment of the culture device of the present invention. Figure 3 is a diagram of a culture tank employed in the culture device of the present invention. FIG. 10... Culture tank, 12... Bypass line, 14.
...pump, 16...inflow pipe, 18...vertical axis of culture tank) 6 Figure 2 Figure 3
Claims (5)
に対して角度を持たせて設け、該培養物流入路から培養
物を培養槽内に流入させ、それによって培養槽内の培養
物を渦状に撹拌させることを含む培養物撹拌方法。(1) A culture inflow path is provided at the bottom of the culture tank at an angle with respect to the vertical axis of the culture tank, and the culture is allowed to flow into the culture tank from the culture inflow path. A culture stirring method comprising stirring a culture in a vortex.
して角度を持たせて設けられた培養物流入路と、該培養
物流入路から前記培養槽内に培養物を流入させる手段と
を含む培養装置。(2) A culture tank, a culture inflow path provided at the bottom of the culture tank at an angle with respect to the vertical axis of the culture tank, and a culture inflow path into the culture tank. A culture device comprising: means for causing inflow.
記載の装置。(3) Claim 2, wherein a plurality of said culture inflow channels are provided.
The device described.
は３記載の装置。(4) The device according to claim 2 or 3, wherein the angle is between 10 degrees and 50 degrees.
て培養槽の上部から下部に至るバイパスラインが通って
おり、該バイパスラインが培養槽の下部に至る部分が前
記流入路であり、前記培養物流入路から前記培養槽内に
培養物を流入させる手段は前記バイパスラインに挿入さ
れたポンプである請求項２ないし４のいずれか１項に記
載の装置。(5) The culture tank is a closed tank, and a bypass line passes through the outside of the culture tank from the top to the bottom of the culture tank, and the part where the bypass line reaches the bottom of the culture tank is the inflow path. 5. The apparatus according to claim 2, wherein the means for flowing the culture into the culture tank from the culture inflow path is a pump inserted into the bypass line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12146588A JP2615394B2 (en) | 1988-05-18 | 1988-05-18 | Culture stirring method and culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12146588A JP2615394B2 (en) | 1988-05-18 | 1988-05-18 | Culture stirring method and culture apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01291784A true JPH01291784A (en) | 1989-11-24 |
| JP2615394B2 JP2615394B2 (en) | 1997-05-28 |
Family
ID=14811812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12146588A Expired - Lifetime JP2615394B2 (en) | 1988-05-18 | 1988-05-18 | Culture stirring method and culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2615394B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011211981A (en) * | 2010-03-31 | 2011-10-27 | Honda Motor Co Ltd | Stirring apparatus |
| JP2019068773A (en) * | 2017-10-10 | 2019-05-09 | 高砂熱学工業株式会社 | Algae culture apparatus |
| JP2021000052A (en) * | 2019-06-24 | 2021-01-07 | 高砂熱学工業株式会社 | Algae-culturing tank system and algae-culturing method |
-
1988
- 1988-05-18 JP JP12146588A patent/JP2615394B2/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011211981A (en) * | 2010-03-31 | 2011-10-27 | Honda Motor Co Ltd | Stirring apparatus |
| JP2019068773A (en) * | 2017-10-10 | 2019-05-09 | 高砂熱学工業株式会社 | Algae culture apparatus |
| JP2021000052A (en) * | 2019-06-24 | 2021-01-07 | 高砂熱学工業株式会社 | Algae-culturing tank system and algae-culturing method |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2615394B2 (en) | 1997-05-28 |
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