JPH05304943A - Device for culturing organism - Google Patents
Device for culturing organismInfo
- Publication number
- JPH05304943A JPH05304943A JP4110987A JP11098792A JPH05304943A JP H05304943 A JPH05304943 A JP H05304943A JP 4110987 A JP4110987 A JP 4110987A JP 11098792 A JP11098792 A JP 11098792A JP H05304943 A JPH05304943 A JP H05304943A
- Authority
- JP
- Japan
- Prior art keywords
- gas
- culture
- culture solution
- permeable membrane
- stirring blade
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、動物細胞や植物細胞等
のように、攪拌等の物理的な力によってダメージを受け
易い生物の培養装置に関わるもので、特に、このような
生物を培養する際に、生物の培養に必要な酸素や二酸化
炭素等のガスを培養槽内に効率よく供給すると同時に、
培養液を均一にしかも培養生物に攪拌によるダメージを
与えることなく混合・攪拌が行えることができる生物の
培養装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a culturing apparatus for organisms such as animal cells and plant cells, which are easily damaged by physical force such as agitation. In doing so, at the same time efficiently supplying gases such as oxygen and carbon dioxide necessary for culturing organisms into the culture tank,
The present invention relates to an apparatus for culturing organisms, which can mix and agitate a culture solution uniformly without causing damage to the cultivated organisms.
【０００２】[0002]
【従来の技術】動物細胞や植物細胞等は、有用物質の生
産等に広く利用されている。これらの動物細胞や植物細
胞等の生物の培養装置の一つとして、ガスを培養槽内に
通気して攪拌翼で培養液を攪拌・混合する形式の通気攪
拌型の培養槽を持った培養装置が利用されている。この
通気攪拌型の培養槽によって、生物の培養に必要な酸素
や二酸化炭素等のガスを積極的に培養液に供給し、培養
液を均一に混合することによって、効率良く培養しよう
とするものである。2. Description of the Related Art Animal cells, plant cells and the like are widely used for producing useful substances. As one of the culture devices for organisms such as animal cells and plant cells, a culture device having an aeration and stirring type culture tank of a type in which gas is aerated and the culture solution is stirred and mixed with a stirring blade. Is used. This aeration-stirring type culture tank positively supplies gases such as oxygen and carbon dioxide necessary for culturing organisms to the culture solution, and uniformly mixes the culture solution to efficiently culture. is there.
【０００３】いわゆる通気攪拌型の培養槽は、一般の微
生物のように攪拌等の物理的な力によってダメージを受
けにくい生物の培養には広く利用されているが、ダメー
ジを受け易い動物細胞や植物細胞の培養にそのまま適用
すると、培養槽に供給されるガスの気泡自体や攪拌翼に
よる剪断力によってこれらの細胞のダメージを引き起こ
してしまい培養することができない。The so-called aeration and agitation type culture tank is widely used for culturing organisms which are not easily damaged by physical force such as agitation like general microorganisms, but animal cells and plants which are easily damaged When it is directly applied to cell culture, the cells themselves cannot be cultured because the gas bubbles supplied to the culture tank themselves or the shearing force of the stirring blades cause damage to these cells.
【０００４】そこで、このようなダメージを受け易い生
物の培養には、次のようないくつかの改良がなされてい
る。例えば、供給するガスを気泡の形で直接培養液に供
給するのではなく、ガス透過膜を培養槽内に設置してガ
スを培養液に溶解させて供給する方式がとられている。
この方式の具体例には、チューブ状のガス透過膜を螺旋
状に編んで培養槽内壁に固定・設置したもの、あるい
は、培養槽内底面に平面状のガス透過膜を固定して設置
したものがある。Therefore, the following improvements have been made in culturing organisms that are susceptible to such damage. For example, instead of directly supplying the gas to be supplied to the culture solution in the form of bubbles, a method has been adopted in which a gas permeable membrane is installed in the culture tank and the gas is dissolved in the culture solution and supplied.
Specific examples of this method include a tube-shaped gas permeable membrane spirally braided and fixed and installed on the inner wall of the culture tank, or a flat gas permeable membrane fixed and installed on the inner bottom surface of the culture tank. There is.
【０００５】[0005]
【発明が解決しようとする課題】しかしながら、これら
のガス透過膜を有する培養槽では、ガス透過膜が培養槽
内に固定されて配置されているので、ガス透過膜表面か
ら培養液への効率よいガス溶解能を維持するためには、
ガス透過膜表面を培養液で洗い流して培養液を流動させ
る必要がある。このガス透過膜表面の培養液の流動には
攪拌翼による比較的強い攪拌が必要とされるが、攪拌が
強いと細胞へのダメージを引き起こしてしまい、また、
攪拌が弱いとガス透過膜表面を培養液で十分に洗い流す
ことができず、ガスの供給が十分に行えないという問題
点がある。However, in the culture tank having these gas permeable membranes, since the gas permeable membrane is fixedly arranged in the culture tank, it is possible to efficiently transfer the gas permeable membrane surface to the culture solution. To maintain the gas dissolving ability,
It is necessary to wash the surface of the gas permeable membrane with the culture solution to allow the culture solution to flow. The flow of the culture solution on the surface of the gas permeable membrane requires relatively strong stirring with a stirring blade, but strong stirring causes damage to cells, and
If the stirring is weak, there is a problem that the surface of the gas permeable membrane cannot be sufficiently washed with the culture solution, and the gas cannot be sufficiently supplied.
【０００６】一方、細胞へのダメージを小さくするため
の技術として、攪拌翼の改良がある。このような目的の
ために使用される攪拌翼は、例えば、従来の攪拌型培養
槽における高い回転数で回転させるための平羽タービン
翼のような形状の攪拌翼とは違って、低回転数で攪拌し
て培養液をマイルドに混合するための表面積の大きい攪
拌翼である。On the other hand, as a technique for reducing damage to cells, there is improvement of a stirring blade. The stirring blade used for such a purpose has a low rotation speed unlike a stirring blade having a shape like a flat blade turbine blade for rotating at a high rotation speed in a conventional stirring culture tank. It is a stirring blade with a large surface area for mildly mixing the culture solution by stirring.
【０００７】この表面積の大きい攪拌翼を上記のガス透
過膜が固定された培養槽に配置することが考えられる
が、表面積を大きく改良した攪拌翼での細胞へのダメー
ジを与えない程度の攪拌程度は、培養槽内の液流速が小
さいため、培養槽内に固定されたガス透過膜表面を洗い
流す程度の流速とすることができず、ガス透過膜表面か
らのガス透過能の維持が困難になるという問題点があ
る。It is considered that the stirring blade having the large surface area is arranged in the culture tank to which the gas permeable membrane is fixed, but the stirring blade having the greatly improved surface area does not damage the cells. Since the liquid flow velocity in the culture tank is low, the flow velocity cannot be such that the surface of the gas permeable membrane fixed in the culture tank is washed out, and it becomes difficult to maintain the gas permeability from the surface of the gas permeable membrane. There is a problem.
【０００８】本発明は以上のような諸問題に鑑みてなさ
れたもので、動物細胞や植物細胞等の物理的ダメージを
受け易い生物の培養において、培養液をマイルドに攪拌
するとともに生物にダメージを与えることなく、培養槽
内にガスを効率よく供給することのでき、さらに動物細
胞や植物細胞等の大量培養、並びにそれらの細胞から有
用物質の効率的生産を実現することができる生物の培養
装置を提供することを目的とする。The present invention has been made in view of the above problems, and in culturing an organism such as an animal cell or a plant cell which is easily physically damaged, the culture solution is gently stirred and the organism is damaged. A device for cultivating organisms that can efficiently supply gas into a culture tank without giving it, and can realize large-scale culture of animal cells, plant cells, etc., and efficient production of useful substances from those cells. The purpose is to provide.
【０００９】[0009]
【課題を解決するための手段】上記した問題点を解決す
るために本発明は、培養生物にダメージを与えずに培養
液の水面下の実質的な大部分を攪拌することのできる大
きさの攪拌翼であって、ガス透過膜が固定されて取り付
けられた攪拌翼を備えた培養槽を有することを特徴とす
る生物培養装置とするものである。In order to solve the above-mentioned problems, the present invention has a size such that a substantial part of the culture solution below the water surface can be stirred without damaging the culture organism. The biological culturing apparatus is characterized by having a culturing tank equipped with a stirring blade, which is a stirring blade and has a gas permeable membrane fixed thereto.
【００１０】また本発明は、培養生物にダメージを与え
ずに培養液の水面下の実質的な大部分を攪拌することの
できる大きさの攪拌翼であって、大部分がガス透過膜の
材質で構成される攪拌翼を備えた培養槽を有することを
特徴とする生物培養装置とするものである。Further, the present invention is a stirring blade having a size capable of stirring substantially a substantial portion of the culture solution under the water surface without damaging the cultured organism, and most of the stirring blade is made of a gas permeable membrane material. The biological culturing apparatus is characterized by having a culturing tank provided with a stirring blade constituted by.
【００１１】[0011]
【作用】本発明による動物細胞や植物細胞等の培養装置
においては、攪拌翼に取り付けられたガス溶解膜から培
養液中に、所望のガスの気泡を発生することなく、直接
溶解させることができる。さらにガス溶解膜は、表面積
の大きい攪拌翼上に固定して設置されているか、又は攪
拌翼の表面の大部分がガス溶解膜で形成されているの
で、攪拌翼が細胞にダメージを与えないような低回転数
のマイルドな条件下で回転しても、培養液が充分に均一
に混合されると同時に、ガス溶解膜表面が常に培養液に
よって洗い流されるのに十分な流速が得られ、結果的に
膜の目詰まりや、ガスの溶解性能の低下をきたすことな
く動物細胞や植物細胞等を効率よく培養できる。In the apparatus for culturing animal cells, plant cells and the like according to the present invention, a desired gas can be directly dissolved in a culture solution from a gas-dissolving film attached to a stirring blade without generating bubbles of a desired gas. .. Furthermore, the gas-dissolving film is fixedly installed on the stirring blade with a large surface area, or most of the surface of the stirring blade is formed by the gas-dissolving film, so that the stirring blade does not damage the cells. Even when the culture solution is rotated under mild conditions such as low rotation speed, the culture solution is mixed sufficiently uniformly, and at the same time, the flow rate is sufficient to wash the surface of the gas-dissolved membrane with the culture solution. It is possible to efficiently cultivate animal cells, plant cells, etc. without causing membrane clogging and deterioration of gas dissolution performance.
【００１２】[0012]
【実施例１】本実施例１を図１及び図２に基づいて説明
する。図１は、チューブ状のガス透過膜が取り付けられ
ている攪拌翼が培養槽の内部に設置されている生物培養
装置の全体図を示し、図２は、図１のＡ−Ａ’断面図で
ある。[Embodiment 1] Embodiment 1 will be described with reference to FIGS. FIG. 1 is an overall view of a biological culture device in which a stirring blade having a tubular gas permeable membrane is installed inside a culture tank, and FIG. 2 is a cross-sectional view taken along the line AA ′ of FIG. is there.
【００１３】図１及び図２において、３は培養槽であ
り、所定容量を有する反応器としての機能をもつ。培養
槽３内には培養液１０が収容される。２は培養槽３内の
培養液１０を攪拌するための攪拌翼であり、培養生物に
ダメージを与えずに培養液１０の水面下の実質的な大部
分を攪拌できるように大面積となっている。。この攪拌
翼２は、培養槽３の外部に設けた攪拌モータ５により駆
動されるシャフト１２を通じて回転する。この攪拌翼２
は、従来の攪拌翼に比べて表面積が大きいため低回転数
の攪拌で培養液１０を均一に混合することができ、従っ
て、培養する生物に対して物理的なダメージの極めて少
ないマイルドな攪拌が実現できる。In FIGS. 1 and 2, reference numeral 3 denotes a culture tank having a function as a reactor having a predetermined capacity. A culture solution 10 is contained in the culture tank 3. Reference numeral 2 denotes a stirring blade for stirring the culture solution 10 in the culture tank 3, which has a large area so that a substantial portion of the culture solution 10 below the water surface can be stirred without damaging the cultured organisms. There is. .. The stirring blade 2 rotates through a shaft 12 driven by a stirring motor 5 provided outside the culture tank 3. This stirring blade 2
Has a large surface area as compared with a conventional stirring blade, so that the culture solution 10 can be uniformly mixed with stirring at a low rotation speed, and therefore mild stirring that causes little physical damage to the organism to be cultured can be achieved. realizable.
【００１４】１はチューブ状のガス透過膜であり、攪拌
翼２の全体を均一に巡るように、攪拌翼２に配置し固定
されている。ガス供給管６には培養槽３の外部から、培
養に必要な空気、酸素、二酸化炭素等のガスが無菌フィ
ルタ１３により無菌のガスとして供給され、培養液１０
中に透過して溶解する。ガス透過膜１は、シリコンやテ
フロンあるいは他の材質等でできており、さらに所望の
ガスだけを選択的に透過する材質の膜などを使用するこ
ともできる。尚、ガス透過膜１の材質、膜の穴径、膜の
厚さ、膜の形状等は、溶解させるガスの種類によって最
適なものを採用する。Reference numeral 1 denotes a tubular gas permeable membrane, which is arranged and fixed on the stirring blade 2 so as to uniformly surround the entire stirring blade 2. Gas, such as air, oxygen, and carbon dioxide, required for culture is supplied to the gas supply pipe 6 from the outside of the culture tank 3 as a sterile gas by a sterile filter 13, and the culture solution 10
Permeates into and dissolves. The gas permeable membrane 1 is made of silicon, Teflon, or another material, and it is also possible to use a membrane made of a material that selectively permeates only a desired gas. The material of the gas permeable membrane 1, the hole diameter of the membrane, the thickness of the membrane, the shape of the membrane and the like are optimally selected depending on the type of gas to be dissolved.
【００１５】培養槽３の上部には、ガスの溶解後の残り
の排ガスを排出するためのガス排出管７が設けられてい
る。排ガスは、ガス透過膜１の培養槽３内に設けられた
排出端に取り付けられたフィルタ１４からでて、培養槽
３の上部気相を通って、無菌フィルタ１３を抜けてガス
排出管７から系外に排出される。さらにこの培養槽３の
上部には、生物を培養するために必要な培地を投入する
ための培地投入管８が設けられている。一方、培養槽３
の下部には使用した培地を排出するための培養液排出管
９が設けられている。A gas discharge pipe 7 for discharging the exhaust gas remaining after the dissolution of the gas is provided above the culture tank 3. Exhaust gas is emitted from the filter 14 attached to the discharge end provided in the culture tank 3 of the gas permeable membrane 1, passes through the upper gas phase of the culture tank 3, passes through the aseptic filter 13, and is discharged from the gas discharge pipe 7. It is discharged outside the system. Further, a culture medium feeding pipe 8 for feeding a culture medium necessary for culturing organisms is provided above the culture tank 3. On the other hand, the culture tank 3
A culture solution discharge pipe 9 for discharging the used medium is provided in the lower part of the.
【００１６】本実施例１の培養装置を用いた培養を説明
する。培養槽３内の攪拌翼２が回転すると、培養液１０
が攪拌されると同時に、ガス透過膜１の表面に対して培
養液１０が相対的に流れる状態となるので、ガス透過膜
１の表面は培養液１０によって常に洗浄される。このた
めに、ガス透過膜１の目詰まり等が起こりにくく、高い
ガスの溶解効率が維持される。Culture using the culture apparatus of Example 1 will be described. When the stirring blade 2 in the culture tank 3 rotates, the culture solution 10
At the same time that the liquid is stirred, the culture solution 10 is allowed to flow relatively to the surface of the gas permeable membrane 1, so that the surface of the gas permeable membrane 1 is always washed with the culture solution 10. Therefore, the gas permeable membrane 1 is less likely to be clogged, and high gas dissolution efficiency is maintained.
【００１７】図１及び図２には、攪拌翼２が平板状で示
されているが、上記の形状に限られず、例えば、複数枚
の平板を組み合わせても良く、又螺旋形のスクリュー羽
根としてもよく、培養する生物に合わせて種々の形式の
攪拌翼が適用できる。また、図１及び図２に示されてい
るように攪拌効果を増すために、適宜に攪拌翼２に中空
部を空けたり、培養槽３の内壁にバッフル板４を設けて
もよい。1 and 2, the stirring blade 2 is shown in the form of a flat plate, but it is not limited to the above-mentioned shape, and for example, a plurality of flat plates may be combined, or a spiral screw blade may be used. Alternatively, various types of stirring blades can be applied according to the organism to be cultured. Further, as shown in FIGS. 1 and 2, a hollow portion may be appropriately provided in the stirring blade 2 or a baffle plate 4 may be provided on the inner wall of the culture tank 3 in order to enhance the stirring effect.
【００１８】また、図１及び図２にはガス透過膜１をチ
ューブ状にしたものを攪拌翼２に固定した例を示した
が、このような形状の膜をこのような方式で固定するこ
とに限ったものではなく、本発明の趣旨に基づき他の形
状の膜や、固定方式を適用することは可能である。さら
に、ガス透過膜１の中のガス流れ方向１１は、図１に示
した方向に限らない。図１及び図２には特に図示してい
ないが、培養温度を一定に保つためには、培養槽３の周
囲に恒温に保たれた加熱媒体等を循環させたジャケット
を設けることができる。Further, FIGS. 1 and 2 show an example in which the tube-shaped gas permeable membrane 1 is fixed to the stirring blade 2. However, a membrane having such a shape may be fixed by such a method. However, it is possible to apply a film having another shape or a fixing method based on the spirit of the present invention. Furthermore, the gas flow direction 11 in the gas permeable membrane 1 is not limited to the direction shown in FIG. Although not particularly shown in FIGS. 1 and 2, in order to keep the culture temperature constant, a jacket in which a heating medium or the like kept at a constant temperature is circulated can be provided around the culture tank 3.
【００１９】[0019]
【実施例２】本実施例２を図３及び図４に基づいて説明
する。図３は、平板状のガス透過膜の材質で構成された
攪拌翼が培養槽の内部に設置されている生物培養装置の
全体図を示し、図４は、図３のＡ−Ａ’断面図である。
本実施例２は、攪拌翼の構成が実施例１と異なり、そ
の他の構成は実施例１と同じである。実施例１と同じ構
成部分については、図１及び図２に示されている番号と
同一の番号が付されており、同じ機能を有する。Second Embodiment A second embodiment will be described with reference to FIGS. 3 and 4. FIG. 3 is an overall view of a biological culture device in which a stirring blade made of a flat plate gas permeable membrane material is installed inside a culture tank, and FIG. 4 is a sectional view taken along line AA ′ of FIG. Is.
In the second embodiment, the structure of the stirring blade is different from that of the first embodiment, and the other structures are the same as those of the first embodiment. The same components as those in the first embodiment have the same numbers as those shown in FIGS. 1 and 2 and have the same functions.
【００２０】２２は培養槽３内の培養液１０を攪拌する
と同時に培養に必要なガスを培養液１０中に溶解させる
作用を有する攪拌翼であり、培養生物にダメージを与え
ずに培養液１０の水面下の実質的な大部分を攪拌できる
ように大面積となっている。この攪拌翼２２は、表面の
主たる部分がシリコンやテフロン等の材質のガス透過膜
２１で形成されており、攪拌の際の強度に耐えられるよ
うに、淵枠、補強枠等の枠材によって補強されている。
その枠材は金属等の剛性の材料で形成されている。ま
た、ガス透過膜２１は、内部がガス通路となっており、
培養装置の外部から無菌フィルタ１３及びガス供給管６
によって培養に必要なガスが導入されており、ガスの流
れ方向は例えば図３で示す矢印１１となり、ガス透過膜
２１の表面から培養液１０中にガスを溶解させて供給し
ている。Reference numeral 22 is a stirring blade having a function of stirring the culture solution 10 in the culture tank 3 and at the same time dissolving the gas required for culture in the culture solution 10, which is a stirring blade of the culture solution 10 without damaging the cultured organisms. It has a large area so that a substantial portion of the water surface can be stirred. A main part of the surface of the stirring blade 22 is formed of a gas permeable film 21 made of a material such as silicon or Teflon, and is reinforced by a frame material such as a pool frame or a reinforcing frame so as to withstand the strength during stirring. Has been done.
The frame material is formed of a rigid material such as metal. The gas permeable membrane 21 has a gas passage inside,
Aseptic filter 13 and gas supply pipe 6 from the outside of the culture device
The gas required for culturing is introduced, and the flow direction of the gas is, for example, an arrow 11 shown in FIG. 3, and the gas is dissolved and supplied from the surface of the gas permeable membrane 21 into the culture solution 10.
【００２１】なお、ガス透過膜２１は所望のガスだけを
選択的に透過する材質の膜を使用することもできる。
尚、ガス透過膜２１の材質、膜の穴径、膜の厚さ、膜の
形状等は、溶解させるガスの種類によって最適なものを
採用する。図３及び図４には、攪拌翼２２が平板状で示
されているが、上記の形状に限られず、例えば、複数枚
の平板を組み合わせても良く、又螺旋形のスクリュー羽
根としてもよく、培養する生物に合わせて種々の形式の
攪拌翼が適用できる。また、実施例１と同様に攪拌効果
を増すために、図３及び図４に示されているように、適
宜、攪拌翼２２に中空部を空けたり、培養槽３の内壁に
バッフル板４を設けてもよい。The gas permeable membrane 21 may be made of a material which selectively permeates only a desired gas.
The material of the gas permeable membrane 21, the hole diameter of the membrane, the thickness of the membrane, the shape of the membrane, etc. are optimally selected depending on the type of gas to be dissolved. Although the stirring blade 22 is shown as a flat plate shape in FIGS. 3 and 4, the stirring blade 22 is not limited to the above shape, and for example, a plurality of flat plates may be combined, or a spiral screw blade may be used. Various types of stirring blades can be applied according to the organism to be cultured. Moreover, in order to increase the stirring effect as in Example 1, as shown in FIGS. 3 and 4, a hollow portion is appropriately provided in the stirring blade 22, or the baffle plate 4 is provided on the inner wall of the culture tank 3. It may be provided.
【００２２】この攪拌翼２２は、実施例１と同様に培養
槽３の外部に設けた攪拌モータ５により駆動されるシャ
フト１２を通じて回転する。この攪拌翼２２は、従来の
攪拌翼に比べて表面積が大きいため低回転数の攪拌で培
養液１０を均一に混合することができ、従って、培養す
る生物に対して物理的なダメージの極めて少ないマイル
ドな攪拌が実現できる。The stirring blade 22 rotates through the shaft 12 driven by the stirring motor 5 provided outside the culture tank 3 as in the first embodiment. Since the stirring blade 22 has a large surface area as compared with the conventional stirring blade, the culture solution 10 can be uniformly mixed by stirring at a low rotation speed, and therefore physical damage to an organism to be cultured is extremely small. Mild stirring can be achieved.
【００２３】[0023]
【発明の効果】以上のように本発明によれば、ガス溶解
膜は、表面積の大きい攪拌翼上に固定されて設置されて
いるか、又は攪拌翼の表面の大部分がガス溶解膜で形成
されているので、攪拌翼が細胞にダメージを与えないよ
うな低回転数のマイルドな条件下で回転しても、培養液
が充分に均一に混合されると同時に、ガス溶解膜表面が
常に培養液によって洗い流されるのに十分な流速が得ら
れ、結果的に膜の目詰まりや、ガスの溶解性能の低下を
きたすことなく動物細胞や植物細胞等を効率よく培養で
きる。したがって、動物細胞や植物細胞等の攪拌による
ダメージを受けやすい生物を効率よく大量に培養でき、
さらにこれらの生物を培養することにより、有用物質の
効率的な生産が実現できる。As described above, according to the present invention, the gas dissolving film is fixedly installed on the stirring blade having a large surface area, or most of the surface of the stirring blade is formed by the gas dissolving film. Therefore, even if the stirring blade is rotated under mild conditions of low rotation speed so as not to damage the cells, the culture solution is mixed sufficiently evenly and at the same time, the surface of the gas dissolving membrane is always As a result, a flow rate sufficient to be washed out can be obtained, and as a result, animal cells, plant cells, etc. can be efficiently cultured without causing clogging of the membrane and deterioration of the gas dissolution performance. Therefore, a large amount of organisms that are easily damaged by agitation such as animal cells and plant cells can be efficiently cultured,
Further, by culturing these organisms, efficient production of useful substances can be realized.
【図１】実施例１のチューブ状のガス透過膜が取り付け
られている攪拌翼が培養槽の内部に設置されている生物
培養装置の全体図である。FIG. 1 is an overall view of a biological culture device in which a stirring blade to which a tubular gas permeable membrane of Example 1 is attached is installed inside a culture tank.
【図２】図１のＡ−Ａ’断面図である。FIG. 2 is a cross-sectional view taken along the line A-A ′ of FIG.
【図３】実施例２の平板状のガス透過膜の材質で構成さ
れた攪拌翼が培養槽の内部に設置されている生物培養装
置の全体図を示す。FIG. 3 is an overall view of a biological culture apparatus of Example 2 in which a stirring blade made of the material of a flat plate gas permeable membrane is installed inside a culture tank.
【図４】図３のＡ−Ａ’断面図である。4 is a cross-sectional view taken along the line A-A ′ of FIG.
１，２１ ガス透過膜 ２，２２ 攪拌翼 ３ 培養槽 ４ バッフル板 ５ 攪拌モータ ６ ガス供給管 ７ ガス排出管 ８ 培地投入管 ９ 培養液排出管 １０ 培養液 １１ ガス流れ方向 １２ シャフト １３ 無菌フィルタ １４ フィルタ 1, 21 Gas-permeable membrane 2, 22 Stirring blade 3 Culture tank 4 Baffle plate 5 Stirring motor 6 Gas supply pipe 7 Gas discharge pipe 8 Medium feeding pipe 9 Culture liquid discharge pipe 10 Culture liquid 11 Gas flow direction 12 Shaft 13 Sterile filter 14 filter
Claims (2)
水面下の実質的な大部分を攪拌することのできる大きさ
の攪拌翼であって、ガス透過膜が固定されて取り付けら
れた攪拌翼を備えた培養槽を有することを特徴とする生
物培養装置。1. An agitator having a size capable of agitating a substantial portion of the culture solution below the surface of water without damaging the organism to be cultivated, the agitator having a gas permeable membrane fixed thereto. A biological culturing apparatus having a culturing tank equipped with wings.
水面下の実質的な大部分を攪拌することのできる大きさ
の攪拌翼であって、大部分がガス透過膜の材質で構成さ
れる攪拌翼を備えた培養槽を有することを特徴とする生
物培養装置。2. A stirring blade having a size capable of stirring substantially a substantial part of the culture solution below the water surface without damaging the cultured organisms, which is mostly composed of a material of a gas permeable membrane. A biological culture apparatus having a culture tank equipped with a stirring blade.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4110987A JPH05304943A (en) | 1992-04-30 | 1992-04-30 | Device for culturing organism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4110987A JPH05304943A (en) | 1992-04-30 | 1992-04-30 | Device for culturing organism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH05304943A true JPH05304943A (en) | 1993-11-19 |
Family
ID=14549538
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4110987A Withdrawn JPH05304943A (en) | 1992-04-30 | 1992-04-30 | Device for culturing organism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH05304943A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004535826A (en) * | 2001-07-31 | 2004-12-02 | アーハードット，ウルスラ | Bioreactor with device whose wall is deformable |
| JP2005348672A (en) * | 2004-06-11 | 2005-12-22 | Ishikawajima Harima Heavy Ind Co Ltd | Cell-culturing apparatus and cell-culturing method |
| KR100656333B1 (en) * | 2005-04-27 | 2006-12-13 | 한국과학기술원 | Power amplifier with automatically switching facility |
| WO2009037683A1 (en) * | 2007-09-17 | 2009-03-26 | Seamus Devlin | A system and apparatus for growing cultures |
| CN102234606A (en) * | 2010-05-04 | 2011-11-09 | 刘惟明 | Simple microbe culture equipment capable of killing bacteria and culture method thereof |
| JP2015116519A (en) * | 2013-12-17 | 2015-06-25 | 佐竹化学機械工業株式会社 | Agitation device |
| JP2016021908A (en) * | 2014-07-18 | 2016-02-08 | 佐竹化学機械工業株式会社 | Reciprocating agitation and culture apparatus having culture medium extracting function |
-
1992
- 1992-04-30 JP JP4110987A patent/JPH05304943A/en not_active Withdrawn
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004535826A (en) * | 2001-07-31 | 2004-12-02 | アーハードット，ウルスラ | Bioreactor with device whose wall is deformable |
| JP2005348672A (en) * | 2004-06-11 | 2005-12-22 | Ishikawajima Harima Heavy Ind Co Ltd | Cell-culturing apparatus and cell-culturing method |
| JP4561192B2 (en) * | 2004-06-11 | 2010-10-13 | 株式会社Ｉｈｉ | Cell culture device and cell culture method |
| KR100656333B1 (en) * | 2005-04-27 | 2006-12-13 | 한국과학기술원 | Power amplifier with automatically switching facility |
| WO2009037683A1 (en) * | 2007-09-17 | 2009-03-26 | Seamus Devlin | A system and apparatus for growing cultures |
| CN102234606A (en) * | 2010-05-04 | 2011-11-09 | 刘惟明 | Simple microbe culture equipment capable of killing bacteria and culture method thereof |
| JP2015116519A (en) * | 2013-12-17 | 2015-06-25 | 佐竹化学機械工業株式会社 | Agitation device |
| JP2016021908A (en) * | 2014-07-18 | 2016-02-08 | 佐竹化学機械工業株式会社 | Reciprocating agitation and culture apparatus having culture medium extracting function |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5075234A (en) | Fermentor/bioreactor systems having high aeration capacity | |
| SK299992A3 (en) | Complex mixer for dispersion of gases in liquid | |
| JPS63317074A (en) | Fermentation apparatus | |
| JP2000317488A (en) | Device for underwater aeration and agitation | |
| FI128860B (en) | Bioreactors for growing micro-organisms | |
| CA2057308A1 (en) | Nonhomogeneous centrifugal film bioreactor | |
| CA2327150C (en) | Apparatus for mixing and aerating liquid-solid slurries | |
| JP2001276878A (en) | Submerged aerating and stirring device | |
| JPH05304943A (en) | Device for culturing organism | |
| CN217127402U (en) | Fermentation device with air distributor | |
| JP2776723B2 (en) | Stirred fermenter | |
| JPH05292942A (en) | Method for culturing using sintered metallic element and its apparatus | |
| RU2099413C1 (en) | Apparatus for suspension cultivation of tissue cells or microorganisms | |
| CN207243878U (en) | Vertical type solid bioreactor | |
| KR950007622B1 (en) | Air injecting device of cell cultivator | |
| CN108728357A (en) | A kind of cell culture apparatus | |
| CN112143651B (en) | Integrated rotary stirring filter for cell culture | |
| SU1730135A1 (en) | Apparatus for growing cells or tissues | |
| CN215924951U (en) | Multifunctional complete mixing bioreactor | |
| JPH078230B2 (en) | Cell culture device | |
| JPH0779677B2 (en) | Incubator | |
| RU2223312C2 (en) | Biological object cultivating apparatus | |
| JP2002239585A (en) | Underwater aeration stirrer | |
| JPS59183687A (en) | Culture method and apparatus of microorganism in nutrition solution | |
| JPH0350715Y2 (en) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 19990706 |