JPH01254864A - Dry type chemical analysis slide and production thereof - Google Patents

Abstract

PURPOSE:To assemble a slide with high accuracy and good efficiency by placing a flexible plane member having layers consisting of thermal adhesive agents between flat plate members consisting of a thermoplastic resin and laminating these members, then adhering the same. CONSTITUTION:Double-face adhesive tapes 2, 3 are superposed with release paper on the outer side onto both faces of a hoop material 1 consisting of the thermoplastic resin (polystyrene, etc.) and after the hoop material 1 and the tapes 2, 3 are adhered by using an ultrasonic horn 4, square apertures of a prescribed size are continuously opened thereto by a punch. The release paper of the tape 3 is then stripped and the hoop material 7 is superposed on the above-mentioned hoop material. The hoop materials 1, 7 are adhered by an ultrasonic horn 8 and the circular holes of a prescribed size are continuously provided to the prescribed positions thereof by a punch 9. The circular holes of the prescribed size are similarly provided continuously to the hoop material 13 by a punch 12 and the release paper of the tape 2 is stripped, then three sheets of the tape materials 13, 1, 7 are adhered thereon over the entire surface by an ultrasonic horn 14. Further, chemical analysis film chips 10 are fixed to the hoop materials 13 and 7 and thereafter, the materials are cut by a cutter 15, by which the slides 16 are obtd.

Description

DETAILED DESCRIPTION OF THE INVENTION "Field of Industrial Application" The present invention relates to a dry analytical element for quantitatively analyzing a specific component in a liquid and a method for manufacturing the same. In particular, dry chemical analysis elements for constant analysis of metabolic substances, metabolic promoting or suppressing substances, etc. in body fluids such as blood using electrical, optical, etc. detection methods and for use in medical diagnosis, and their gJ Regarding the manufacturing method.

[Prior art and its disadvantages] Such soft chemical analysis elements are known from Japanese Patent Publication No. 53-21677, Japanese Patent Application Laid-open No. 55-164356, and the like. They are placed on a water-impermeable light-transparent support -m
Layers such as a reagent layer, a liquid-extended I7I layer, etc. are provided, and it is often referred to as a chemical analysis film. Since it is difficult to collect the sample liquid as it is for spotting and optical measurement, the frame described in Japanese Patent Publication No. 57-28331, Japanese Patent Application Laid-Open No. 57-63452, Jitsuma 1+RL No. 56-142454, etc., is a so-called mount. used in the form of chemical analysis slides.

The components of such mounts are often made of thermoplastic resins such as polystyrene, polyethylene, polypropylene, or thermoset resins such as phenol-formaldehyde resin, and these mounts have a high degree of positional accuracy during optical measurements. Because of these requirements, each member is often manufactured by injection molding of thermoplastic resin or molding of thermosetting resin, but the molds used for molding are expensive to manufacture.

Further, when assembling these members, it is necessary to ultrasonically bond the members in order to obtain precision. −
To assemble the mount using molded parts,
Production efficiency is low because assembly work must be done one by one.

[Technical Problems to be Solved] The technology to be solved by the present invention is to manufacture mounts for chemical analysis slides that require high dimensional accuracy at a relatively low cost and to improve the efficiency of assembling chemical analysis slides. This is a major issue.

[Means for solving the technical problem] The solution to the above problem is to provide a flexible work surface having a layer made of a thermoadhesive adhesive between two or more flat members made of thermoplastic resin or thermosetting resin. A mount (a frame for mounting a dry analysis element) is formed by integrating the two or more flat plate members and the flexible work surface member by stacking the members and then thermally bonding them. This was achieved by dry chemical analysis slides with

In addition, a mount for attaching a dry analysis element such as a dry chemical analysis film is placed between two or more flat members made of thermoplastic resin or thermosetting resin, with a layer made of thermoadhesive adhesive on both sides. The two or more flat plate members and the flexible work surface member are integrally formed by stacking and thermally adhering flexible board members having the same structure, thereby forming a dry chemical analysis slide. Achieved by a manufacturing method.

Specific Structure of the Invention] The flat member used in the present invention can be made of various known thermoplastic resins, such as polyethylene, polypropylene, polyvinyl chloride, polyvinylidene chloride, polystyrene, polycarbonate, polyethylene terephthalate, In addition to the above polymers, a known plasticizer may be included, such as cellulose acetate, cellulose acetate propionate, and cellulose acetate Pitriate.

The flat member used in the present invention may be made of a thermosetting resin commonly used for molding, such as phenol (
phenol formaldehyde) resin, xylene resin, urea resin, melamine resin, polyurethane resin, p-hydroxyammonium r'! - Aroma IgQ resin, polyimide resin, furan resin, vinyl ester resin, diallyl phthalate resin, guanamine resin, etc. can be used. These details can be found, for example, in "Product Dictionary" published by Toyo Keizai Inc. (1976), pages 671 to 676, "Chemical Products" r9285, published by Kaji Kogyo Nippo Publishing Co., Ltd. (1985), page 67.
It is described from page 5 to page 689.

The flexible work surface member used in the present invention includes paper, cloth, nonwoven fabric,
Various known thermoplastic resins (M resin, etc.) are used, and have a heat-adhesive layer on both sides. Examples of the thermoplastic resins include polyethylene, polypropylene, polyvinyl chloride, polyvinylidene chloride, polycarbonate, polyethylene terephthalate, and cellulose acetate. In addition to the polymers mentioned above, a known plasticizer such as cellulose acetate propionate may be included.

Two surfaces of the flexible working surface member used in the present invention have a layer of thermal adhesive. As the thermoadhesive adhesive, a hot melt material as described in Industrial Materials v1, Volume 26, No. 11, pages 4 to 5 can be used.

That is, ethylene copolymers such as ethylene-vinyl acetate copolymer, ethylene-ethyl acrylate copolymer, and ethylene-acrylic acid copolymer, polyolefins such as low molecular weight polyethylene and atactic polypropylene, and polyamides such as nylon. , polyester copolymer, SBS
Various materials can be used, such as thermoplastic rubbers such as styrene block copolymers, styrene-butadiene rubber, butyl rubber, urethane rubber, rosin, petroleum resins, terpene resins, paraffins, synthetic waxes, etc.

Such a layer of thermal adhesive can be provided on the surface of the flexible working surface member by a known method such as doctor coating or curtain coating.

Whichever side has the thermal adhesive J- on one or two sides,
It may also have a release paper or a release liner.

As a double-sided thermal adhesive tape with a thermal adhesive layer on both sides, Kurashiki Boseki Co., Ltd.'s tJ's ``Krumpeter J'', Daicel Corporation's
tJ's [Diamide Film] and the like are known.

The shape of the flat plate part of the chemical analysis slide mount of the present invention is not particularly limited, but a square or rectangle is suitable for handling the slide. It may be hexagonal, hexagonal, circular, oval, etc.

In the flat member, an opening 11 is provided as necessary, especially at a position corresponding to the center or near the center of the mount. 1 for contacting the potential measurement probe with the square or circular opening of ) or the terminal of the sheet electrode.
The middle member has an opening corresponding to the shape of the chemical analysis film for accommodating the chemical analysis film, and the uppermost member has an opening for supplying a liquid to be measured such as a sample liquid to the chemical analysis slide. If necessary, these openings are also provided in the double-sided thermal bonding member.

As an intermediate member, a member having an opening for accommodating a distribution member for distributing the liquid to be measured from the liquid supply hole to the chemical analysis slide (for example, member 33 of JP-A-58-211648)
), a member provided with an opening for supplying the liquid to be measured from the liquid distribution member to the chemical analysis slide (for example, JP-A-58-211
No. 648 member 30), a member for housing or fixing a bridge providing electrical continuity between the sample solution and the 9-irradiation solution (
For example, member 52) of Japanese Patent Application Laid-Open No. 62-9264 may be provided.

In order to thermally bond laminated members through a thermally adhesive layer, heat conduction from the outside of the outermost layer using a solid, liquid, or gas heat source may be used, or high frequency (electromagnetic wave) induction or M-sonic waves may be used. Heat generation may also be used.

In order to apply ultrasonic waves to the laminated members, an ultrasonic horn of a known method such as magnetostrictive vibration can be used. The shape of the horn is not particularly limited either, but a horn with a flat tip, a cone-shaped horn, etc. can be used. As for the frequency of the ultrasonic waves, any known appropriate frequency can be used, and there is no particular limitation.

It is possible to easily obtain values necessary and sufficient for adhesion with respect to the energy L, Jn time, etc. of the ItfIa wave through a preliminary test.

When using heat conduction using a solid as a heat source, it is desirable to use a heat source with a temperature lower than the softening temperature of the thermoplastic resin or thermosetting resin.

The chemical analysis slide to which the present invention is applied can have various shapes.
No. 52, JP-A-62-9264, JP-A-02-3
No. 9757, JP-A No. 19758, JP-A-62-111655, JP-A-62-93650,
JP-A-62-93651, JP-A-62-93852, JP-A-62-168047, JP-A-62-15
The present invention can be applied to the chemical analysis slides described in No. 2256 and the like.

According to the method of the present invention, members having required openings can be laminated and integrated with the required mutual precision of the members and openings easily and with high efficiency. The method of the present invention is particularly effective when using a long web of a thermoplastic flat member, so-called a hoop material. Since the necessary processing can be applied to the pendant hat, slides can be assembled with high precision and efficiency.

The present invention will be explained in more detail below using Examples.

[Example 1] A schematic cross-sectional view of the apparatus used in this example is shown in FIG.

Mainly made of polystyrene, thickness 0.8m, width 28m
A width of 28 wheels m0 is placed on both sides of the hoop material (long web) la.
Double-sided thermal adhesive tape "Crumpeter" No. 1430 (manufactured by Kurashiki Boseki 1FF Co., Ltd.) 2a and 3a are stacked with the release paper on the outside, and the tips are rectangular with a distance of 261 m II in the width direction of the hoop material and 2211 m in the length direction. Using four conical ultrasonic horns 4 located at the apexes, ultrasonic waves with a frequency of 20 kHz are applied to the hoop material and two double-sided thermal adhesive tapes at four points each at a pitch of 24III11 in the length direction. Hot glued.

Thereafter, Bunch et al. continuously opened a square opening with a side of 16 mm centered at the center of the rectangle formed by the four glued points (intersection of diagonals).

Peel off the release paper 6a of the lower thermal adhesive tape, and then
That is, after stacking a polystyrene hoop material (second hoop material) 7a with a thickness of 0.3 feet and a width of 281 mm on the lower side, the tip is 24 mm in the width direction of the hoop material, and the ends are separated by 20 mm in the length direction. Four conical ultrasonic horns 8 located at the vertices of a rectangle
The two hoop materials 1a and 7a were bonded together via the thermal adhesive tape 3a by applying ultrasonic waves with a frequency of 20 kHz to the inner side of the bonding position at four points every 24+ am using a .

Two hoop materials 1a are attached via a thermal adhesive tape 3a.

7a was attached. Of the two hoop materials stacked in this way, the lower hoop material 7a is centered at a position corresponding to the center of the opening (intersection of diagonals) of the upper hoop material 1a.
A circular hole with a diameter of 1011 # was continuously made using a bunch 9.

In the depression formed by the opening of the upper hoop material 1a,
- A square glucose analysis film (chip) 10 with sides of 15 mm was inserted so that the colored layer was on the bottom side.

Separately, a third polystyrene hoop material 13a with a thickness of 0.3 mm and a width of 28II11 was placed with a center spacing of 24m5 and a diameter of 10mm.
A bunch 12 was used to continuously drill circular holes with a diameter of mm.

Then, the release paper 11a of the upper thermal adhesive tape 2a is
was peeled off, and a third hoop material 13a was placed thereon so that the centers of the circular openings coincided with each other.

In the center of a rectangular plane of 24 x 2811 II11, the height is 1.2 where the tip is located at the vertex of a square with a side of 10 pieces.
Using an ultrasonic horn 14 with a conical protrusion of 1 flll, ultrasonic waves at a frequency of 20 kHz are applied so that its center coincides with the center of the circular opening, so that the flat part of the horn is brought into close contact with the hoop material on the U side. Press the protrusions into the three hoop members 13a and 13a until they are pressed.

7a and two double-sided thermal adhesive tapes 2a and 3ae were thermally bonded over the entire surface except for the opening, and the chemical analysis film chip 10 was fixed to the hoop materials 13a and 7a.

The Wt, IIl band thus produced was cut into a 24 mm x 28+ am rectangle with a cutter 15, with the circular opening at the center, to complete a chemical analysis slide 16.

[Example 2] Instead of the double-sided thermal adhesive tape "Krumpeter J No. 1430" used in Example 1, [Krumpeter, 436
A chemical analysis slide was prepared in the same manner as in Example 1 except that No. 0 was used.

[Example 3] The hoop material and the thermal adhesive tape had a width of 56111 mm, ultrasonic bonding and perforation were performed in two rows each in the width direction, and the resulting laminate was cut into 24 x 56 + IIm.
The same procedure was used for Al. The 24×56mM fragment was further cut into 24×28m+m pieces using a cutter and used as a chemical analysis slide.

[Example 4] In this example, the first
Instead of the hoop material, a molded phenol formaldehyde resin plate with a thickness of 0.8 mm is used, and in place of the second and third hoop materials, a molded phenol formaldehyde resin plate with a thickness of 0.3 mm is used, Chemical analysis slides were prepared.

Double-sided thermal adhesive tape "Crumpeter" 143 with a width of 28T1
No. 0 (manufactured by Kurashiki Boseki) Layer 2a and 3a so that the release paper is on the outside, and sandwich a phenol formaldehyde resin plate 1a molded to 24 x 28 mm and thickness 0.81 between the two tapes, and the tip is 26 mm x Using four conical ultrasonic horns 4 located at the vertices of a 22+1M rectangle,
Ultrasonic waves at a frequency of 20 kHz were applied to thermally bond the double-sided thermal adhesive tape near the top of the W fingerboard.

Thereafter, a square opening with a side of 16 mm centered at the center of the rectangle formed by the four bonded points (intersection of diagonals) was continuously opened using the bunch 5.

After peeling off the release paper 6a of the lower thermal adhesive tape and overlaying a molded phenol formaldehyde resin plate 7a of 24×28IIm-thickness 0.3mm on that surface, 24I1
Using four cone-shaped ultrasonic pawns 8 located at the vertices of a 1 m x 20 mm rectangle, ultrasonic waves with a frequency of 20 kHz are applied to the inside of the bonding position at four points each, and the two sheets are bonded via the thermal adhesive tape 3a. The resin plates 1a and 7a were bonded together.

The upper resin plate 1 of the two resin plates layered on top in this way
A circular hole with a diameter of 10 mm was made in the lower resin plate 7a using a bunch 9, centering on a position corresponding to the center of the opening (intersection of diagonal lines) a.

In the depression formed by the opening of the upper resin plate 1a, -
A square glucose analysis film (dep) JO with sides of 15 mm was inserted with the coloring layer facing down.

Separately, a circular hole with a diameter of 10 m1+1 was made with a bunch 12 in the center of a phenol formaldehyde resin plate 13a molded to a size of 24×2811III+0.3 mm thick.

Then, the release paper 11a of the upper thermal adhesive tape 2a was peeled off, and the resin plate 13a was stacked on top of the strip so that the centers of the circular openings coincided with each other.

In the center of a rectangular plane of 24 x 28 mm, the tip is located at the vertex of a square with a side of 10 - 1.21 fiI.
Using an ultrasonic horn 14 with a conical protrusion of m, ultrasonic waves at a frequency of 20 kHz are applied so that the center of the square coincides with the center of the circular opening, so that the flat part of the horn comes into close contact with the upper resin plate. Press-fit the protrusions until the three resin plates 13a and la are attached.

7a and two double-sided thermal adhesive tapes 2a and 3a were thermally bonded over the entire surface except the 1 m opening, and the chemical analysis film chip 10 was fixed to the resin plates 13a and 7a.

The laminate was cut from the thermal adhesive tape with a cutter 15 to complete a chemical analysis slide 16.

[Brief explanation of the drawing]

FIG. 1 is a schematic diagram showing a cross section of an apparatus used in Example 1 of the present invention. FIG. 2 is a cross-sectional view of a chemical analysis slide prepared according to each embodiment of the present invention. In FIG. 1, 1 is a supply section for hoop material 1a, 2 and 3 are supply sections for double-sided adhesive tapes 2a and 3a, and 11 and 6 are supply sections for double-sided adhesive tapes 2a and 3a, respectively.
7 and 13 indicate the supply sections of the hoop materials 7a and 13a, respectively. FIG. 3 is a schematic cross-sectional view of the device used in Example 4. In FIG. 3, 1 is a supply part for the resin plate 1a, 2 and 3 are supply parts for double-sided adhesive tapes 2a and 3a, and 11 and 6 are supply parts for double-sided adhesive tapes 2a and 3a, respectively.
7 and 13 indicate supply sections for the resin plates 7a and 13a, respectively.

Claims (5)

[Claims] (1) Two or more flat members made of thermoplastic resin or thermosetting resin are laminated with a flexible flat member having a layer of thermoadhesive adhesive on both sides interposed therebetween, and the two or more sheets A dry chemical analysis slide comprising a mount in which a flat plate member and the flexible flat member are integrated. (2) A method for manufacturing a dry chemical analysis slide in which a dry analysis element is attached to a mount, wherein a thermoadhesive adhesive is applied on both sides between two or more flat members made of thermoplastic resin or thermosetting resin. The mount is formed by integrating the two or more planar members and the flexible planar member by stacking and thermally bonding flexible planar members having layers consisting of the above. A method for manufacturing dry chemical analysis slides. (3) By laminating and thermally bonding a flexible planar member having a layer of heat-adhesive adhesive on both sides between two or more planar members made of thermosetting resin, 2. The method of manufacturing a dry chemical analysis slide according to claim 2, wherein the mount is formed by integrating the two or more flat plate members and the flexible flat member. (4) The manufacturing method according to claim (2), characterized in that thermal bonding is performed by applying ultrasonic waves. (5) A method for manufacturing a dry chemical analysis slide in which a dry analysis element is attached to a mount, wherein two or more flat members made of thermoplastic resin or thermosetting resin are thermally bonded on both sides between the members. The method is characterized in that the mount is formed by sandwiching and laminating flexible planar members having a layer made of a flexible adhesive and thermally bonding them, and the dry analysis element is attached to the mount during the formation of the mount. A method for manufacturing dry chemical analysis slides.