JPH0121960B2 - - Google Patents
Info
- Publication number
- JPH0121960B2 JPH0121960B2 JP2954081A JP2954081A JPH0121960B2 JP H0121960 B2 JPH0121960 B2 JP H0121960B2 JP 2954081 A JP2954081 A JP 2954081A JP 2954081 A JP2954081 A JP 2954081A JP H0121960 B2 JPH0121960 B2 JP H0121960B2
- Authority
- JP
- Japan
- Prior art keywords
- bacteria
- methylumbelliferyl
- microorganisms
- hours
- fluorescent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241000894006 Bacteria Species 0.000 claims description 55
- 238000000034 method Methods 0.000 claims description 42
- 244000005700 microbiome Species 0.000 claims description 37
- 238000012360 testing method Methods 0.000 claims description 25
- ORHBXUUXSCNDEV-UHFFFAOYSA-N umbelliferone Chemical class C1=CC(=O)OC2=CC(O)=CC=C21 ORHBXUUXSCNDEV-UHFFFAOYSA-N 0.000 claims description 15
- 230000000813 microbial effect Effects 0.000 claims description 12
- HXVZGASCDAGAPS-UHFFFAOYSA-N 4-methylumbelliferyl acetate Chemical compound CC1=CC(=O)OC2=CC(OC(=O)C)=CC=C21 HXVZGASCDAGAPS-UHFFFAOYSA-N 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 8
- -1 4-methylumbelliferyl arabinoside Chemical class 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 3
- 239000012085 test solution Substances 0.000 claims description 3
- BCHIXGBGRHLSBE-UHFFFAOYSA-N (4-methyl-2-oxochromen-7-yl) dihydrogen phosphate Chemical compound C1=C(OP(O)(O)=O)C=CC2=C1OC(=O)C=C2C BCHIXGBGRHLSBE-UHFFFAOYSA-N 0.000 claims description 2
- YUDPTGPSBJVHCN-CHUNWDLHSA-N 4-methylumbelliferyl alpha-D-galactoside Chemical compound C1=CC=2C(C)=CC(=O)OC=2C=C1O[C@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O YUDPTGPSBJVHCN-CHUNWDLHSA-N 0.000 claims description 2
- YUDPTGPSBJVHCN-DZQJYWQESA-N 4-methylumbelliferyl beta-D-galactoside Chemical compound C1=CC=2C(C)=CC(=O)OC=2C=C1O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O YUDPTGPSBJVHCN-DZQJYWQESA-N 0.000 claims description 2
- 235000011180 diphosphates Nutrition 0.000 claims description 2
- 229930182478 glucoside Natural products 0.000 claims 1
- DLFVBJFMPXGRIB-UHFFFAOYSA-N thioacetamide Natural products CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 claims 1
- 150000008505 β-D-glucopyranosides Chemical class 0.000 claims 1
- HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 description 33
- 239000002609 medium Substances 0.000 description 31
- 239000000243 solution Substances 0.000 description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 21
- 238000006243 chemical reaction Methods 0.000 description 18
- 235000013305 food Nutrition 0.000 description 11
- 239000000523 sample Substances 0.000 description 11
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 10
- 229920001817 Agar Polymers 0.000 description 9
- 239000008272 agar Substances 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 9
- 241000588724 Escherichia coli Species 0.000 description 8
- 241000233866 Fungi Species 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 239000008363 phosphate buffer Substances 0.000 description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 238000007796 conventional method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000001802 infusion Methods 0.000 description 6
- 239000008101 lactose Substances 0.000 description 6
- FHHPUSMSKHSNKW-SMOYURAASA-M sodium deoxycholate Chemical compound [Na+].C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 FHHPUSMSKHSNKW-SMOYURAASA-M 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 239000001888 Peptone Substances 0.000 description 5
- 108010080698 Peptones Proteins 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 235000019319 peptone Nutrition 0.000 description 5
- 235000021067 refined food Nutrition 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 239000000411 inducer Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000002351 wastewater Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000012921 fluorescence analysis Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 238000013207 serial dilution Methods 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- CJIJXIFQYOPWTF-UHFFFAOYSA-N 7-hydroxycoumarin Natural products O1C(=O)C=CC2=CC(O)=CC=C21 CJIJXIFQYOPWTF-UHFFFAOYSA-N 0.000 description 2
- 241000304886 Bacilli Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 101100391812 Escherichia phage Mu gam gene Proteins 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 240000008415 Lactuca sativa Species 0.000 description 2
- 241000235648 Pichia Species 0.000 description 2
- 241000588769 Proteus <enterobacteria> Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 102000005936 beta-Galactosidase Human genes 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 229960001506 brilliant green Drugs 0.000 description 2
- HXCILVUBKWANLN-UHFFFAOYSA-N brilliant green cation Chemical compound C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 HXCILVUBKWANLN-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
- 229940097572 chloromycetin Drugs 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 229960003964 deoxycholic acid Drugs 0.000 description 2
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 2
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 2
- 239000012470 diluted sample Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000012029 potato salad Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 235000012045 salad Nutrition 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 238000003911 water pollution Methods 0.000 description 2
- ARQXEQLMMNGFDU-JHZZJYKESA-N 4-methylumbelliferone beta-D-glucuronide Chemical compound C1=CC=2C(C)=CC(=O)OC=2C=C1O[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O ARQXEQLMMNGFDU-JHZZJYKESA-N 0.000 description 1
- ARQXEQLMMNGFDU-UHFFFAOYSA-N 4MUG Natural products C1=CC=2C(C)=CC(=O)OC=2C=C1OC1OC(C(O)=O)C(O)C(O)C1O ARQXEQLMMNGFDU-UHFFFAOYSA-N 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- 241000590020 Achromobacter Species 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 244000099147 Ananas comosus Species 0.000 description 1
- 235000007119 Ananas comosus Nutrition 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000589565 Flavobacterium Species 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000192041 Micrococcus Species 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 241000187654 Nocardia Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000006364 Torula Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 150000001540 azides Chemical class 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013611 frozen food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000012569 microbial contaminant Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 235000014214 soft drink Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000011410 subtraction method Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- HFTAFOQKODTIJY-UHFFFAOYSA-N umbelliferone Natural products Cc1cc2C=CC(=O)Oc2cc1OCC=CC(C)(C)O HFTAFOQKODTIJY-UHFFFAOYSA-N 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
- 235000020681 well water Nutrition 0.000 description 1
- 239000002349 well water Substances 0.000 description 1
- 239000007218 ym medium Substances 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2954081A JPS57144995A (en) | 1981-03-02 | 1981-03-02 | Quick test of microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2954081A JPS57144995A (en) | 1981-03-02 | 1981-03-02 | Quick test of microorganism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS57144995A JPS57144995A (en) | 1982-09-07 |
| JPH0121960B2 true JPH0121960B2 (fr) | 1989-04-24 |
Family
ID=12278940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2954081A Granted JPS57144995A (en) | 1981-03-02 | 1981-03-02 | Quick test of microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS57144995A (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1470883A (en) * | 1982-04-14 | 1983-11-04 | Unilever Plc | Microbiological test processes and apparatus |
| JPH0411899A (ja) * | 1990-04-27 | 1992-01-16 | Shimadzu Corp | 生物体の計数法 |
| JP4510222B2 (ja) * | 2000-04-26 | 2010-07-21 | 三菱化学メディエンス株式会社 | 細菌の鑑別方法 |
-
1981
- 1981-03-02 JP JP2954081A patent/JPS57144995A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS57144995A (en) | 1982-09-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4591554A (en) | Rapid method for detecting microorganisms | |
| Manafi | Fluorogenic and chromogenic enzyme substrates in culture media and identification tests | |
| JP3043063B2 (ja) | 微生物のための沈澱テスト | |
| JP3041312B2 (ja) | サルモネラ属の細菌を検出するための細菌学的分析方法及び培地 | |
| JP3034036B2 (ja) | 水中のｅ．ｃｏｌｉを同定するための新規および改良された方法 | |
| AU2008220705B2 (en) | Bacteria detection and/or identification medium | |
| Delisle et al. | Rapid detection of Escherichia coli in urine samples by a new chromogenic beta-glucuronidase assay | |
| CN108048522A (zh) | 具有对碳青霉烯类的抗性的细菌的检测 | |
| US4070247A (en) | Diagnostic media | |
| CN101970682B (zh) | 检测和/或鉴别艰难梭状芽孢杆菌的方法 | |
| JPH0472517B2 (fr) | ||
| KR101747347B1 (ko) | 바실러스 세레우스 그룹으로부터 박테리아를 검출하는 방법 | |
| JP2010517551A (ja) | 細菌を検出および／または同定するための培地 | |
| JP5845179B2 (ja) | β‐ラクタム抗生物質に対して耐性化しているグラム陰性菌の特異的検出のための培地 | |
| CN103443288A (zh) | 具有对碳青霉烯类的酶促抗性的细菌的检测 | |
| CN104508140B (zh) | 检测产生oxa-048碳青霉烯酶的细菌的方法 | |
| US9404141B2 (en) | Method for detecting the presence or absence of a target microbe in a test sample | |
| EP3094738B1 (fr) | Utilisation d'au moins un substrat de carboxylestérase et/ou de triacylglycérol-lipase pour la détection des bactéries du groupe bacillus cereus | |
| JPH0121960B2 (fr) | ||
| JP2004507252A (ja) | サンプル中の標的微生物検出のための組成物および方法 | |
| JP5882330B2 (ja) | Ｃ．ｄｉｆｆｉｃｉｌｅを検出および／または同定するための、β−グルコシダーゼ活性化剤の使用 | |
| JP5823390B2 (ja) | 新規ニトロレダクターゼ酵素基質 | |
| JP5730305B2 (ja) | 新規ニトロレダクターゼ酵素基質 | |
| JP2002010799A (ja) | 細菌検査培地 | |
| JP5730304B2 (ja) | 新規ニトロレダクターゼ酵素基質 |