JPH01207226A - Cosmetic for hair - Google Patents

Abstract

PURPOSE:To obtain a hair cosmetic which contains, as an active ingredient, beta-lipotropin, thus being effective for blackening gray hairs, for preventing hairs from whitening by aging with very high safety. CONSTITUTION:The subject hair cosmetic contains, as an active ingredient, beta-lipotropin, a peptide hormone which is excreted from the middle lobe of pituitary and is composed of 90-91 amino acids by 0.0001-0.1wt.%. beta-Lipotropin promotes the activation and propagation of chromogens in hair matrix and increases the tyrosinase activity, and accelerates to transfer melamine granules from dendrites of the chromoblast to the cortical cells in the hair matrix, thus developing the blackening effect.

Description

Detailed Description of the Invention [Field of Industrial Application] The present invention relates to hair cosmetics (medicated cosmetics as quasi-drugs and external hair preparations as pharmaceuticals), which are characterized by containing β-lipotrophin as an active ingredient. (including (the same applies hereinafter)).

[Conventional technology]

Graying of human hair is recognized as a typical phenomenon of the physiological aging process. In addition, gray hair is clinically known as a symptom of many skin diseases, such as vitiligo, albinism, and alopecia areata. It may be possible to prevent this. In addition, hair graying due to malnutrition and hair graying due to chronic systemic diseases have also been reported, and in mice, vitamin B, complex,
Experiments have been conducted on hair whitening by two meals deficient in p-aminobenzoic acid, and it has been observed that white hair turns black by administering large amounts of p-aminobenzoic acid, but this has not been applied to humans.

At present, there is no fundamental drug treatment for graying hair as a physiological aging phenomenon, and the only method of cosmetic treatment is to dye gray hair with hair dye.

The use of hair dye is simply a passive means of hiding gray hair, and its effects are temporary, often accompanied by hair damage, and cases of allergic contact dermatitis are also common. .

[Problem that the invention seeks to solve]

According to Mösher et al., ``Shiraga'' may begin to appear in the 20s to 30s, but it usually occurs in the 40s.
It often begins to occur in people in their 50s. The frequency of gray hair at each age is 25% for both men and women between the ages of 25 and 35, 50% at the age of 50, and almost 100% at the age of 80a (
Mo5her D, B,, In″Derm, in G
eneral Med,” McGraw-Hill
Co., p. 605, 1979) As is clear from these statistical data, despite the great social demand for treatment or preventive measures against hair graying, no effective drug has been developed at present. Not yet.

The present inventors therefore aimed to provide a new hair cosmetic that is effective in turning gray hair black, has a preventive effect against hair whitening due to aging, etc., and is safe.

[Problem and means to solve it]

Melanin formation in hair occurs when colorless amino acids,
Melanin granules (melanocytes) formed from tyrosine
The formed melanin granules are ingested into the hair cortex cells through the dendrites of pigment cells, move upward with hair growth, and determine the color tone of the hair.

Regarding the developmental mechanism of gray hair, Fitzpatrick et al. have shown the possibility of changes in the pigment cell system as follows. (F
itzpatrick T, +In +Advance
es in Biology of 5kin+Per
Gamon Press, p35.1965) ■Decrease in the number of pigment cells in the hair matrix ■The number of pigment cells in hair H remains unchanged, but due to a decrease in tyrosine oxidase ■Tyrosine oxidation process due to the presence of pigment formation inhibiting substances produced in the body ■ Disappearance of active tyrosine oxidase sites due to morphological changes in melanin granules ■ Decrease in melanin granules ■ Inhibition of migration of melanin granules to epidermal cells ■ Melanocyte mother that is thought to differentiate into pigment cells present in the asci Cellular Changes ■ Lack of Pigment Cell Nm Gun However, few of these possibilities have been demonstrated.

The present inventors have focused on the importance of the action of β-lipotrophin in activating pigment-forming cells, and have devoted themselves to research for many years regarding the application of this hormone to hair cosmetics.

β-lipotrophin is reported to be a peptide hormone consisting of 90 to 91 amino acids secreted from the intermediate lobe of the pituitary gland in animals. In mammals, its secretion increases especially in the summer, making it easier for melanin production to occur due to ultraviolet rays. However, no research has been conducted on the incorporation of β-lipotrophin into hair cosmetics and its usefulness.

The present inventors prepared a hair cosmetic containing β-lipotrophin as an active ingredient, and as a result of examining its effect on hair, it was found that it is extremely effective for turning gray hair black, and that it is effective against whitening hair due to aging, etc. found that it has a preventive effect against
Furthermore, we confirmed its high safety and completed the present invention.

In the present invention, β-lipotrophin promotes the activation and proliferation of pigment-forming cells in the hair matrix, increases tyrosinase activity, and promotes the movement of melanin granules from the dendrites of pigment cells to the cortical cells of the hair. , it was thought that the effect would be hailed.

That is, the present invention provides a novel hair cosmetic containing β-lipotrophin as an active ingredient.

As the β-lipotrophin that can be used in the present invention, any β-lipotrophin originating from animals, plants, and fungi can be used, and these β-lipotrophins can be obtained by known methods, such as the method of 5cott et al.
A, P, ,Biochem, J, ,139
, 593, 1974), the method of Li et al. (Li C, J
, , Nature, 208°1093.1965
), the method of Birk et al. (Birk Y., J.
, Biol, Chem, 239.1048.
(1964), the method of C5eh et al. (C5eh G, ,
FEBS Lett, 21.344.1972
) etc. can be used. Additionally, mass production using genetic recombination technology is also possible.

Note that the purity of β-lipotrophin used in the present invention is preferably as high as possible from an immunological point of view, but the purity is not particularly limited. , the amount of purified β-lipotrophin can be changed as appropriate depending on the agent layer, etc., but the amount of purified β-lipotrophin is usually about 0.
．． It is used in a range of 0001 to 0.1% by weight.

The hair cosmetic composition of the present invention may be any agent layer that can be applied externally, such as hair tonic, hair treatment, hair cream, hair shampoo, hair rinse, etc., and the base may be any liquid or solid material that is acceptable for hair application. Shape cosmetic bases can be widely used. Additionally, additives such as preservatives, fragrances, and colorants may be included if desired.

[Effect]

The results of testing the effectiveness and safety of the hair cosmetic of the present invention thus obtained are as follows.

Test example 1. Cultured pigment cell melanization effect test 1.5 x 10' B-16 mouse melanoma cells were placed in a day dish (60 mm diameter) containing 4 m2 of Eagle's MEM medium (containing 10% tallow serum) to evaluate the effectiveness of the hair cosmetic of the present invention. Addition of component β-lipotrophin (0, 1 or 10
μg/mj2 medium) and then cultured for 4 days in a carbon dioxide gas incubator set at 37°C and 15% CO.

After detaching and dispersing the proliferated cells with trypsin, the number of cells was counted, and the degree of blackening of the pigment cells obtained by centrifugation at 1,001,000 rpm was visually evaluated as 6! It has been decided.

In addition, a method of colorimetrically quantifying the increase in doperchrome at 475 nm using L-tyrosine as a substrate for tyrosinase activity in pigment cells (Clinical Skin, 36, 715, 1982)
) was measured.

The results are shown in Table 1, and it can be seen that β-lipotrophin, the active ingredient of the hair cosmetic of the present invention, promotes the proliferation of cultured pigment cells, increases tyrosinase activity, and promotes the production of melanin granules, resulting in blackening. It has become clear that it is effective.

Table 1* Knee: No blackening, +: Slightly blackening, ++: High degree of blackening Test Example 2. Patch test〈Sample〉 0.1% β-lipotrophin aqueous solution〈Operation〉 15 men and 15 women aged 23 to 67 years old, total 3
A closed patch test was performed by attaching a sample-soaked filter paper for a fin chamber to the flexor side of the upper arm of 0 subjects, which had been inserted into the chamber of the fin chamber.

The application time was 24 hours, the fin chamber was removed after 24 hours, and the evaluation was performed twice: 30 minutes and 24 hours after sample removal.

Criteria for evaluation Knee: No reaction at all, ±: Slight erythema, +;
Obvious erythema, ++; erythema and swelling, erythema and papules,
+++; In addition to the above, those with blisters.As shown in Table 2, β-lipotrophin, which is the active ingredient of the hair cosmetic of the present invention, has a very low possibility of causing irritation or allergic reactions, and is highly safe. It became clear that

Table 2 [Examples] Examples of the present invention are shown below, but the present invention is not limited thereto.

Example 1 Kubear tonic> β-lipotrophin 0.002 g Ethanol so, o g Polyoxyethylene hydrogenated castor oil 1. Og glycerin 5.0 g purified water
Appropriate amount Total amount 100.0 g Example 2 <Hair treatment> β-lipotrophin o, os g Stearic acid 3.0 g Liquid paraffin 20.0 g Glycerin monostearate 3.0 g Lanolin alcohol
5.0 g propylene glycol
4.0 g triethanolamine 1
．． 0 g Methylparaben 0.2
gPurified water appropriate amount
100.0 g Example 3 <Hair cream> β-lipotrophin 0.01 g Liquid paraffin 20.0 g Solid paraffin 3.0 g Polyoxyethylene cetyl ethyl 2.0 g Sorbitan sesquioleate 1.0 g Water Potassium oxide 061 g glycerin
3.0 g ethylparaben
0.1 g Purified water Appropriate Total amount 100.0 g Example 4 <Hair shampoo> β-lipotrophin o, ootg Sodium lauryl sulfate 35.0 g Magnesium stearate 4.0 g Polyvinyl alcohol 1.0 g Cetyl alcohol
2.0 g lanolin
1.0 g glycerin laurate 2
．． 0 g Purified water Appropriate total amount 100.0 g Example 5 Hair rinse> β-lipotrophin 0.02 g Diethylene glycol monostearate 3.0 g Distearyldimethylammonium chloride 2. Og Propylene Glyfur 3. Og methyl paraben
0.2 g purified water
Appropriate amount Total amount: 100.0 g [Effects of the invention] As detailed above, the hair cosmetic characterized by containing β-lipotrophin of the present invention as an active ingredient is effective for blackening gray hair and is effective against aging. It has a preventive effect against the whitening of hair caused by hair loss, etc., and is extremely safe, so it is fully expected to be used in clinical applications. Therefore, the industrial significance of the present invention is very large.

Patent applicant: Taiyo Kagaku Co., Ltd.

Claims (1)

[Claims] Hair cosmetics characterized by containing β-lipotrophin as an active ingredient