JP2001122792A - Skin preparation for external use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To find out a new means capable of preventing and ameliorating various kinds of dermatoses, sking roughenings, chappy skins skin agings, etc., accompanied by pigmentation, dermal stains, ephelides and keratinization abnormality after sunburn. SOLUTION: This skin preparation for external use is obtained by including one or more kinds of extracts of plants selected from the group consisting of a plant (Bischofia Bl.) belonging to the genus Bischofia, a plant (Epigaea L.) belonging to the genus Epigaea, a plant (Camptotheca Decne.) belonging to the genus Camptotheca and a plant (Clethra L.) belonging to the genus Clethra as an active ingredient.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an external preparation for skin containing a specific plant extract as an active ingredient, which can be used as an external preparation for whitening, an external preparation for improving skin roughness or an external preparation for preventing skin aging.

[0002]

2. Description of the Related Art One of the expected effects of a skin external preparation is as follows.
It has the effect of preventing and improving spots, freckles, rough skin, skin aging and the like, and a number of skin external preparations such as cosmetics for these purposes have been developed.

Under these circumstances, conventionally, extracts of animals and plants, which are considered to have an anti-inflammatory effect, and amino acids, polysaccharides, lipids, and natural polymers having a high moisturizing and water-retaining effect are used to reduce skin inflammation and associated pigmentation. Prevention, prevention of water loss of the stratum corneum,
Alternatively, it has been used because of its excellent ability to moisturize the skin.

[0004] Recently, the mechanism of occurrence of spots, freckles, rough skin, skin aging and the like has been elucidated biochemically. First, regarding the mechanism of the occurrence of skin spots, it is generally considered that abnormalities in hormones and stimulation of ultraviolet rays from sunlight cause melanin pigment to be formed, which is abnormally deposited in the skin. Melanin pigment, which causes skin coloring, is produced in melanin-producing granules (melanosomes) in melanocytes between the epidermis and dermis, and diffuses to neighboring cells by osmotic action. The biochemical reaction in this melanocyte is estimated as follows.

That is, tyrosine, which is an essential amino acid, is converted into dopaquinone by the action of the enzyme tyrosinase, and the process by which dopaquinone is converted to black melanin via a red pigment and a colorless pigment by enzymatic or non-enzymatic oxidizing action is the melanin pigment. It is estimated as a generation process.

[0006] Therefore, it is considered important to suppress the action of tyrosinase, which is the first step of the reaction, in suppressing the production of melanin. However, a conventional compound that suppresses the action of tyrosinase generally exhibits an extremely slow effect, so that the effect of improving skin pigmentation is not always sufficient. Hydroquinone also has
Although excellent tyrosinase inhibitory action is observed, its use is generally restricted due to its sensitizing properties. Therefore,
In order to improve the safety of hydroquinone, attempts have been made to use derivatives of higher fatty acids such as monoesters and alkyl monoethers (Japanese Patent Application Laid-Open No. 58-154507). Therefore, it is not always safe to say that the ether derivative is not sufficiently satisfactory in terms of safety.

[0007] In recent years, the mechanism of disease image formation of various skin diseases accompanied by rough skin and abnormal keratinization includes protease,
In particular, it has been revealed that changes in serine protease activities such as plasmin and plasminogen activator (PA) are deeply involved.

For example, in psoriasis, which is a typical example of inflammatory dyskeratosis, it is reported that strong PA activity is present at the parakeratotic site of the affected epidermis (Haustein: Arch. Klin. Exp. Dermatol;
234,1969) and psoriasis scales using high-concentration saline
Report that A was extracted (Fraki, Hopsu-Havu: Arch.Derm
atol.Res; 256, 1976). PA is a protease that works specifically on plasminogen, a precursor of plasmin, and converts it into active plasmin.

There is also a report showing that a compound known as a serine protease inhibitor suppressed skin roughness (Kenji kitamura: J. Soc. Cosmet. Chem. Jpn; 29 (2), 1).
995).

Further, the mechanism of skin aging, ie, wrinkles,
Dullness, loss of texture, decreased elasticity, etc. include reduction of dermal matrix fibers such as collagen and elastin,
Alternatively, it is known that denaturation is involved. In recent years, the influence of matrix metalloprotease has been pointed out as a factor inducing this change. Among the matrix metalloproteases, collagenase (matrix metalloprotease: MMP 1) is known as an enzyme that degrades type I and III collagen, which is a main component of the dermal matrix. The expression of collagenase is greatly increased by the irradiation of ultraviolet light, which is considered to be one of the causes of the decrease and denaturation of collagen due to the ultraviolet light and, in turn, one of the causes of the formation of skin wrinkles. ing.

[0011] Therefore, inhibition of collagenase protects the matrix forming fibers and is considered to be an important means for preventing skin aging.

[0012]

The problem to be solved by the present invention is to prevent and improve pigmentation, sun spots, freckles, various skin diseases accompanied by abnormal keratinization, rough and rough skin, skin aging, etc. after sunburn. To find new means to gain.

[0013]

Means for Solving the Problems The present inventors have found that the prevention and improvement of the above-mentioned skin diseases and the like are based on the medicinal components based on the mechanism of occurrence of each skin disease and the like, that is, tyrosinase, serine protease, collagenase and the like. Considering that the component having the enzyme inhibitory effect was effective, various substances were examined widely. As a result, plants belonging to the genus Akagi (Bischofia Bl.) And plants belonging to the genus Iwanashi (Epigaea
L.), a plant belonging to the genus Kanrenboku (Camptotheca Decn.
e.) and a solvent extract of a plant belonging to the genus Ryobu (Clethra L.) have an excellent inhibitory effect on tyrosinase, serine protease, and collagenase, and a skin external preparation further comprising these The present inventors have found that the present invention has an effect of preventing pigmentation after sunburn and preventing and improving skin roughness and wrinkles in winter, and based on this, the present invention has been completed.

That is, the present invention relates to a plant belonging to the genus Akagi (Bischofia Bl.) And a plant belonging to the genus Iwanashi (Epiga).
ea L.), a plant belonging to the genus Kanrenboku (Camptotheca Dec.
ne.) and an extract of one or more plants selected from the group consisting of plants belonging to the genus Ryobu (Clethra L.) as an active ingredient (hereinafter also referred to as the skin external preparation of the present invention). It is the invention which provides.

The skin external preparation of the present invention includes, as specific embodiments, a whitening external preparation (hereinafter, this embodiment is also referred to as the present whitening external preparation) and a skin roughening external preparation (hereinafter, this embodiment is referred to as
An embodiment as the external preparation for preventing rough skin of the present invention) or an external preparation for preventing skin aging (hereinafter, this embodiment is also referred to as an external preparation for preventing aging of the present invention) can be adopted.

To the knowledge of the present inventors, there have been reports on the tyrosinase inhibitory action, serine protease inhibitory action, collagenase inhibitory action of the above plant extracts, and further reports on the application of these plant extracts to external skin preparations. I can't.

In the present invention, the external preparation for skin means an agent which can be widely applied to the outer skin (including the scalp), and is used in cosmetics, pharmaceuticals, quasi-drugs and the like under the Pharmaceutical Affairs Law. There is no restriction on the classification.

In the present invention, the external preparation for preventing skin aging broadly means a preparation having an action of preventing and improving skin aging. Further, the serine protease inhibitory action according to the present invention, plasmin, trypsin,
It means an inhibitory effect on enzymes classified as serine proteases, such as urokinase, tissue-type plasminogen activator, and elastase.

[0019]

Embodiments of the present invention will be described below. The plant (Bischofia Bl.) Belonging to the genus Akagi, which is an active ingredient of the external preparation for skin of the present invention , belongs to the family Euphorbiaceae, and at present, the akagi (Bischofia java)
nica Blume) is a plant of a genus.

Akagi is an evergreen tree that is distributed in Okinawa, the Ogasawara Islands, Taiwan, India, Malaysia and the like. The roots, bark, and leaves of Akagi are used as Chinese herbal medicines referred to as Shufuki in China, but no reports have been found on uses or effects of the present invention.

[0021] The roots, bark, leaves,
An extract of a flower or the like, preferably a leaf or bark extract can be used as an active ingredient of the external preparation for skin of the present invention.
Plants belonging to the genus Sorbus (Epigaea L.) are evergreen shrubs belonging to the ericaceae family and characterized by an enlarged fruit placenta, such as char (Epigaea asiatica Maxim.) And American char (Epigaea repens L.). Is mentioned. In the present invention, particularly, Japanese chard can be suitably used.

[0021] Fruits of plants belonging to the genus Sorbus have been reported to be edible and have a digestive-promoting action, an antipyretic action, and a tonic effect, but no reports have been found on the uses or actions relating to the present invention.

Fruits, leaves, and the like of plants belonging to the genus
Extracts of roots, flowers, etc., preferably extracts of whole flowering plants,
It can be used as an active ingredient of the skin external preparation of the present invention. A plant belonging to the genus Campantheca (Camptotheca Decne.)
Belongs to the dogwood family, and specific examples thereof include Kanrenboku (also referred to as Kiju: Camptotheca acuminata Decne.).

Kanrenboku is a deciduous tree distributed in the mountains in the middle and southern parts of the continent of China. There are reports that anticancer effects are observed on fruits and roots, but no report on the use or action of the present invention is found. .

The bark of a plant belonging to the genus
Extracts of leaves, fruits, roots and the like, preferably extracts of fruits or bark, can be used as the active ingredient of the external preparation for skin of the present invention.

A plant belonging to the genus Ryobu (Clethra L.)
Is a deciduous small tree belonging to the family Ryobu, and about 30 species are found in East Asia and the Americas.
rbinervis Sieb. et Zucc.) is a typical and preferred species in the present invention. In addition, there is no report on the use or action of plants belonging to the genus Ryobu in the present invention.

The leaves, stems and roots of plants belonging to the genus Ryobu
An extract of a flower or the like, preferably an extract of the whole flowering plant, can be used as an active ingredient of the external preparation for skin of the present invention. The extract of each of the above-mentioned plants to be incorporated into the external preparation for skin of the present invention can be obtained by generally known extraction means, for example, after immersing or heating and refluxing the extraction site of these plants with an extraction solvent, followed by filtration and concentration. Can be. As the extraction solvent, any solvent can be used as long as it is a solvent generally used for extraction. For example, alcohols such as methanol and ethanol; organic solvents such as aqueous alcohol, acetone and ethyl acetate are used alone or in combination. be able to.
The extract may be obtained by using the above-mentioned solvent and subjecting it to a treatment such as partitioning or purification such as chromatography.

In the skin external preparation of the present invention, one or more of the above-mentioned plant extracts are selected and used alone or in combination (hereinafter, unless otherwise specified, the active ingredient of the skin external preparation of the present invention) The plant extract described above may be used regardless of the type of plant used.

The amount of the present plant extract in the external preparation for skin of the present invention is preferably 0.005 to 2% by dry weight of the agent, regardless of the type of plant and the specific use of the external preparation.
0.0% by weight, and particularly preferably 0.01 to 1% by weight.
0.0% by weight. If the amount is less than 0.005% by weight of the agent as a dry weight, the effect of the present invention will not be sufficiently exhibited, and if it exceeds 20.0%, formulation tends to be difficult. Also, even if the dry weight exceeds 10.0% by weight of the agent, the effect corresponding to the amount of the compound tends not to be improved.

As described above, by using the present plant extract as an active ingredient, various skin diseases associated with pigmentation, spots, freckles, and abnormal keratinization after tanning, rough and rough skin,
The skin external preparation of the present invention which can prevent and improve skin aging and the like can be obtained.

Specific Aspects of the External Preparation for Skin of the Present Invention When the external preparation for skin of the present invention is actually used, in addition to the plant extract which is an essential component, components usually used for external preparations for skin, for example, humectants Requires antioxidants, oily components, UV absorbers, emulsifiers, surfactants, thickeners, alcohols, powder components, sequestering agents, coloring materials, aqueous components, water, various skin nutritional agents and drugs, etc. Can be appropriately compounded according to the conditions.

The external preparation for skin of the present invention can be applied in any form conventionally used as an external preparation for skin, for example, ointment, cream, milky lotion, lotion, pack, bath preparation, and the like. There is no particular limitation.

As described above, the skin external preparation of the present invention has various excellent effects and can be used as a skin external preparation that exerts these effects comprehensively. It can also be used as an external preparation for skin.

First, paying attention to the skin whitening effect (tyrosinase inhibitory effect) of the present extract, the skin external preparation of the present invention can be used as an external preparation for whitening (the external preparation for whitening of the present invention).

The external preparation for whitening of the present invention can contain the above-mentioned general components in addition to the present extract. In particular, by adding a known whitening component, a further whitening effect can be obtained. Can be demonstrated.

Specifically, vitamin C, magnesium ascorbate phosphate, glucoside ascorbate, arbutin, kojic acid, tranexamic acid, and licorice,
It is possible to mix tea extract and the like. The blending amounts of these known whitening components can be appropriately changed according to the specific blending components, product forms, and the like.

In addition, paying attention to the skin roughening preventing / improving effect (serine protease inhibitory effect) of the present extract, the skin external preparation of the present invention was applied to the skin roughening improving agent (in the present invention, unless otherwise specified, “improvement of skin roughening” , Which is a concept including "prevention of rough skin") (the rough skin improving agent of the present invention).

The above-mentioned general components can be added to the skin roughness improving agent of the present invention in addition to the present extract. In particular, by adding a known skin roughness improving component, a further effect of improving skin roughness can be obtained. Can be demonstrated.

Specifically, humectants such as glycerin, 1,3-butylene glycol, sodium 2-pyrrolidone-5-carboxylate and sodium hyaluronate; sugars such as glucose, fructose, mannose, sucrose, trehalose and xylitol Anti-inflammatory agents such as glycyrrhizic acid, allantoin, azulene; astringents such as citric acid, tannic acid and zinc oxide; cooling agents such as menthol and camphor; various vitamins; antihistamines; It is possible. The compounding amount of these known skin roughness improving components can be appropriately changed according to specific compounding components, product forms, and the like.

Furthermore, focusing on the excellent inhibitory effect of the present extract on collagenase, the skin external preparation of the present invention can be used as an external preparation for preventing skin aging (the external preparation of the present invention for preventing aging).

The external preparation for preventing aging according to the present invention can contain the above-mentioned general components in addition to the present extract. The effect of improving skin roughness can be exhibited.

Specifically, it is possible to add an antioxidant such as thiotaurine, hypotaurine, vitamin E or an ultraviolet absorber. The compounding amount of these known skin aging preventive components can be appropriately changed according to specific compounding components, product forms, and the like.

[0043]

EXAMPLES The present invention will be described in more detail with reference to the following examples, but it is not intended to limit the technical scope of the present invention. Further, the compounding amount is represented by% by weight, and the compounding amount of the plant extract is converted to dry weight unless otherwise specified.

First, a test method for the inhibitory effect of the present plant extract on each of tyrosinase, serine protease, and collagenase, and a test method for a whitening effect, a skin roughness improving effect, an anti-aging effect, and evaluation criteria thereof will be described. 1. Enzyme Inhibition Test (1) Preparation of samples Redwood (Bischofia javanica Blume), Iwanashi (Epiga
ea asiatica Maxim.), Kanrenboku (Camptotheca acum)
inata Decne.), Ryobu (Clethra barbinervis Sieb.)
et Zucc.) 50 g of each leaf and flower (wet weight)
Was immersed in a 5-fold amount of ethanol at room temperature for 1 week, and the extract was concentrated to dryness. The solid obtained was dissolved in dimethylsulfoxide (DMSO) and 10% DMSO of each extract
A solution was prepared. The following experiment was performed using this.

(2) Measurement of Tyrosinase Inhibitory Effect Mouse cultured B16 melanoma cells were cultured in 10% FB
Using Eagle MEM medium containing S and theophylline (0.09 mg / mL), 95% O ₂ , 5% CO ₂ , 37 ° C.
Was cultured in an incubator. After 24 hours of culture,
A sample solution was added to a final concentration of 0.1 or 0.01%, and the culture was continued for another 3 days. Thereafter, the medium was removed, the cells were washed twice with PBS, and then 45 μL of PBS containing 1% Triton X-100 (a surfactant manufactured by Rohm and Haas) was added to each well.

The plate was shaken for 1 minute, and the absorbance at 475 nm was measured with a microplate reader, and this was taken as the absorbance at 0 minutes. Then, quickly add 10 mM to each well
Of L-DOPA solution was added and transferred to an incubator at 37 ° C., and the absorbance was measured again 60 minutes later.

Using the well containing only the DMSO solution instead of the sample solution as a control, the ratio (%) of the difference in absorbance of the sample-added well to the difference in absorbance at 0 and 60 minutes of the control was determined. The subtracted value was defined as the tyrosinase inhibition rate%. The results are shown in Table 1.

As a reference example, the same test as described above was carried out on an ethanol extract of oyster, a plant already known to have a tyrosinase inhibitory action. Table 1 also shows the results.

(3) Measurement of Serine Protease Inhibitory Effect The inhibitory effect of the present extract on plasmin was examined by the fibrin plate method. That is, 6 mL of a veronal buffer solution (25 mmol / L aqueous sodium barbitalate solution containing 0.125 mol / L-NaOH, pH 7.4) containing 1.0% plasminogen-removed fibrinogen is poured into a 9 cm φ petri dish, and 1.0 mol is added thereto. / L -CaCl ₂ 0.
2 mL and 0.1 mL of 25 U / mL thrombin were added, mixed gently, and left for 1 hour. 5 U / m on a plate formed by converting fibrinogen to fibrin
A mixture of L 2 plasmin and the sample solution to a final concentration of 0.1 or 0.01% was added at 37 ° C. for 10 minutes.
After incubation for 20 minutes, 20 μL was added, and the mixture was further incubated at 37 ° C. for 18 minutes.
Left for hours. DMSO instead of test sample as control
And then the area of the dissolved circle formed by dissolution of fibrin was measured, and the plasmin inhibition rate% was determined by the following equation (1). The results are also shown in Table 1.

Further, as a reference example, the same test as described above was conducted on an ethanol extract of pomegranate fruit, which is a plant known to have a plasmin inhibitory action. Table 1 also shows the results.

Inhibition rate (%) = {1- (dissolved circle area of test sample / dissolved circle area of control)} × 100

(4) Measurement of Collagenase Inhibitory Action 100 μL of 0.4 unit / mL human cell-derived collagenase (manufactured by mussel) and 50 μL of 1 mg / mL fluorescein isocyanate-labeled type I collagen (manufactured by mussel)
And a buffer for measurement (0.4 M NaCl, 10 mM) so that the final concentration becomes 0.01 and 0.001%.
50 μL of each sample solution diluted with 0.1 M Tris (pH 7.4 containing CaCl ₂ ) was added and incubated at 37 ° C. for 2-3 hours. As a control, DMSO alone diluted with a measurement buffer was added. Thereafter, ethanol was added to precipitate unreacted collagen, and the fluorescence intensity of the degraded collagen in the supernatant was measured to determine the percent degradation of type I collagen. From the ratio of the degradation rate of the system to which the sample was added to the degradation rate of the control, the collagenase inhibition rate%
I asked. The results are also shown in Table 1. One unit of the enzyme is the amount of the enzyme that degrades 1 μg of collagen per minute.

As a reference example, ethylenediaminetetraacetic acid (E
DTA) was subjected to the same test as described above. Table 1 also shows the results. Table 1 ────────────────────────────────────Inhibition rate (%) sample Final concentration of tyrosinase plasmin Collagenase ──────────────────────────────────── red oak extract 0.1% 50.9 51.1 -0.01% 6.2 28.3 96.3 0.001%--29.0 Japanese radish extract 0.1% 52.5 62.7-0.01% 8.7 23.8 91.5 0.001%--35.0 Kanrenboku 0.1% 57.1 54.8-Extract 0.01% 11.4 29.9 97.5 0.001%--42.7 Ryobu extract 0.0 1% 45.8 48.0-0.01% 4.6 12.2 82.0 0.001% --0 0 oyster extract 0.1% 40.1 -0.01% 1.1--Pomegranate extract 0.1%-28.0-0.01%-10.9-EDTA 0.05%--80.0 0.005%--0── ────────────────────────────────── Note)-indicates that the value has not been evaluated.

As can be seen from Table 1, the plant extracts of Akagi, Iwanashi, Kanrenboku and Ryobu have an inhibitory effect on tyrosinase, plasmin (serine protease) and collagenase enzymes, and their strengths are determined by Kaikai Compared with the tyrosinase inhibitory action of the extract, the plasmin inhibitory action of the pomegranate extract, and the collagenase inhibitory action of EDTA, they were more excellent.

2. Actual use test (1) Preparation of sample The whitening effect, skin roughness improvement effect and wrinkle improvement effect by applying the skin external preparation of the present invention to the outer skin were measured using the leaves and flower parts 50 g (wet weight) of each of Akagi, Iwanashi, Kanrenboku and Ryobu. The sample was immersed in a 50-fold volume of 50% ethanol at room temperature for one week, and evaluated using a sample as shown in Table 2 including an extract obtained by filtration. As a comparative example, a sample containing the same 50% ethanol extract of Yuzuliha (JP-A-7-280663), which is known to have a whitening effect, a skin roughening prevention effect, and a skin aging prevention effect,
A sample containing no plant extract was used.

(2) Whitening Effect Test <Test Method> A 2 × 2 cm square was placed on the inner skin of both upper arms of 30 healthy male subjects exposed to the sunlight of summer for 4 hours (2 hours a day for 2 days). 3 slots per arm and a total of 6 slots as test sites
After the day, the sample was applied to each slot twice a day for 4 weeks.
After 4 weeks, the skin color was visually evaluated based on the following evaluation criteria.

<Judgment Method> The whitening effect of each sample was judged from the evaluation results of skin color based on the following whitening effect judgment criteria. The results are shown in Table 2.

<Skin Color Evaluation Criteria> Significant effect: Clear lightening is observed at the sample application site. Effective: Lightening is observed at the sample application site. Slightly effective: slight lightening is observed at the sample application site. Invalid: the skin color of the sample application site and the surrounding site does not change.

<Criteria for judging whitening effect> A: The ratio (effective rate) at which the subject shows a remarkable effect and an effect is 70
% Or more. ：: 50% (effective rate) showing that the test subject was significantly effective and effective
% To less than 70%. Δ: The ratio of the test subject showing remarkable effect and effectiveness (effective rate) is 30
% To less than 50%. ×: The ratio (effective rate) at which the subject showed a remarkable effect and an effect was 30.
%.

(3) Skin roughness improvement effect test <Test method> A replica of the skin of a healthy woman's face was taken using a replica agent, and the skin surface morphology was observed with a microscope (17 times). From the state of the skin pattern and the peeling state of the stratum corneum, 60 rough skin panels evaluated as 1 or 2 were divided into 6 groups of 10 persons based on the following evaluation criteria, and samples were allocated to each group. . The sample was applied twice a day for 4 weeks,
After a week, the skin condition was observed again according to the replica method described above and scored.

<Determination Method> From the difference between the replica score after 4 weeks and the score at the start of the test (at the time of panel selection), the skin roughness improvement effect of each sample was determined based on the following skin roughness improvement effect determination criteria. The results are shown in Table 2.

<Replica Evaluation Criteria> 1: Elimination of skin sulcus, skin ridges, and turning over of a wide range of stratum corneum are recognized. 2: Skin crevices and skin ridges are unclear and horny layers are turned up. 3: Skin crevices and skin ridges are recognized, but flat. 4: The skin groove and skin hills are clear. 5: The skin sulcus and skin hills are clear and well-organized.

<Criterion for Estimating Skin Roughness Improvement Effect> A: The proportion (effective rate) of subjects having a score difference of 2 or more is 7
0% or more. ：: The proportion (effective rate) of subjects having a score difference of 2 or more is 5
0% or more and less than 70%. Δ: The proportion (effective rate) of subjects having a score difference of 2 or more is 3
0% or more and less than 50%. ×: The proportion (effective rate) of subjects having a score difference of 2 or more is 3
It is less than 0%.

(4) Wrinkle Improvement Effect Test <Test Method> The condition of wrinkles at the corners of the eyes of a healthy woman aged 35 to 50 was visually observed, and scored based on the following wrinkle evaluation criteria. Among them, 120 persons rated as 1 or 2 were divided into 6 groups of 20 persons, and the samples were allocated to each group twice a day for 4 weeks. Four weeks later, the wrinkles at the outer corners of the eyes were observed again and scored.

<Judgment Method> The wrinkle improvement effect of each sample was determined from the difference between the wrinkle score 4 weeks later and the score at the start of the test (at the time of selecting the panel) based on the following criteria for wrinkle improvement effect. The results are shown in Table 2.

<Wrinkle Evaluation Criteria> 1: Many deep wrinkles and fine wrinkles are observed. 2: Slight deep wrinkles and many fine wrinkles are observed. 3: No deep wrinkles are observed, but fine wrinkles are observed. 4: Slight wrinkles are observed. 5: Wrinkles are hardly recognized.

<Criteria for Evaluation of Wrinkle Improvement Effect> A: The proportion (effective rate) of subjects having a score difference of 1 or more is 7
0% or more. ：: The proportion (effective rate) of subjects having a score difference of 1 or more is 5
0% or more and less than 70%. Δ: The proportion (effective rate) of subjects having a score difference of 1 or more is 3
0% or more and less than 50%. ×: The proportion (effective rate) of subjects having a score difference of 1 or more is 3
It is less than 0%.

Table 2 { Comparative product sample cream of the present invention ( % By weight) 1 2 3 4 1 2 1 Red oak extract 2.0 ― ― ― ― ― 2 Sardine extract ― 2.0 ― ― ― ― 3 Kanrenboku extract ― ― 2.0 ― ― ― 4 Ryobu extract ― ― ― 2.0 ― ― 5 Yuriha extract ― ― ― ― 2.0 ― 6 Propylene glycol 10.0 10.0 10.0 10.0 10.0 10.0 7 Stearic acid 5.0 5.0 5.0 5.0 5.0 5.0 8 Stearyl alcohol 4.0 4.0 4.0 4.0 4.0 4.0 9 Isopropyl 18.0 18.0 18.0 18.0 18.0 18.0 Myristate 10 Glycerin mono 3.0 3.0 3.0 3.0 3.0 3.0 Stearic acid ester 11 Caustic potassium 0.2 0.2 0.2 0.2 0.2 0.2 12 Sodium bisulfite 0.01 0.01 0.01 0.01 0.01 0.01 13 Preservative Amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount 14 Ion-exchanged water Residual Residual Residual Residual Residual ────────────────────────────────── ── Whitening effect ◎ ○ ◎ ○ △ × Skin roughness improvement effect ◎ ◎ ◎ ◎ ○ △ Wrinkle improvement effect ○ ○ ○ ○ △ × ────────────────────── ──────────────

<Production Method> Each of plant extracts 1 to 5 and 6, 7 were added to ion-exchanged water, dissolved, and heated to 70 ° C. (aqueous phase). On the other hand, the other components were mixed and melted by heating.
C. (oil phase), and this was gradually added to the aqueous phase. After the addition was completed, the temperature was maintained for a while to cause a reaction. Thereafter, the mixture was uniformly emulsified with a homomixer and cooled to 30 ° C. while stirring well to obtain the creams shown in Table 2.

As can be seen from Table 2, the skin external preparations (creams) of the present invention all exhibited excellent whitening effect, skin roughness improvement effect and wrinkle improvement effect as compared with the comparative products.
Hereinafter, specific formulations of the skin external preparation of the present invention will be described as examples. In addition, when the above-mentioned actual use test was performed on the skin external preparations of these examples, all of the skin external preparations were found to have excellent whitening effect, skin roughness improving effect and wrinkle improving effect. In addition, the ethanol extract used in the above-mentioned practical use test was used as the present extract in these examples, unless otherwise specified.

Example 1 Cream Ingredients Ingredients (% by Weight) Stearic Acid 2.0 Stearyl Alcohol 7.0 Hydrogenated Lanolin 2.0 Squalane 5.0 2-Octyldodecyl Alcohol 6.0 Polyoxyethylene (25 Mol) Cetyl alcohol ether 3.0 Glycerin monostearate 2.0 Propylene glycol 5.0 Akagi methanol extract 0.05 Tranexamic acid 0.2 Sodium bisulfite 0.03 Ethyl paraben 0.3 Perfume Appropriate amount Deionized water Residual <Production method> Add propylene glycol to ion-exchanged water,
Heated and maintained at 70 ° C. (aqueous phase). The other components were mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase was added to the water phase to carry out preliminary emulsification, and after uniform emulsification with a homomixer, the mixture was cooled to 30 ° C. while stirring well to obtain a cream.

[Example 2] Cream blending component blending amount (% by weight) Solid paraffin 5.0 Beeswax 10.0 Vaseline 15.0 Liquid paraffin 41.0 Glycerin monostearate 2.0 Polyoxyethylene (20 mol) Sorbitan monolaurate 2.0 Soap powder 0.1 Borax 0.2 Kanrenboku 70% methanol extract 0.1 Sodium bisulfite 0.03 Ethyl paraben 0.3 Perfume Appropriate amount Ion-exchange water Residue <Production method> Ion exchange Soap powder and borax were added to water and heated to 70 ° C. (aqueous phase). The other components were mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase was gradually added to the aqueous phase while stirring to carry out a reaction. Thereafter, the mixture was uniformly emulsified with a homomixer and cooled to 30 ° C. while stirring well to obtain a cream.

[Example 3] Emulsion component amount (% by weight) Stearic acid 2.5 Cetyl alcohol 1.5 Vaseline 5.0 Liquid paraffin 10.0 Polyoxyethylene (10 mol) Monooleate 2.0 Polyethylene glycol 1500 3.0 Triethanolamine 1.0 Carboxyvinyl polymer 0.05 (trade name: Carbopol 941, BFGoodrich Chemical company) Ethyl acetic acid extract 0.02 Sodium bisulfite 0.01 Ethylparaben 0.3 Fragrance Appropriate amount of ion-exchanged water residue <Production method> The carboxyvinyl polymer was dissolved in a small amount of ion-exchanged water (phase A). Polyethylene glycol 1500 and triethanolamine were added to the remaining ion-exchanged water, and the mixture was heated and dissolved and maintained at 70 ° C. (aqueous phase). The other components were mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase was added to the water phase to perform preliminary emulsification, the phase A was added, and the mixture was uniformly emulsified with a homomixer. After the emulsification, the mixture was cooled to 30 ° C. while stirring well to obtain an emulsion.

Example 4 Emulsion Formulation Component Content (% by weight) Microcrystalline Wax 1.0 Beeswax 2.0 Lanolin 20.0 Liquid Paraffin 10.0 Squalane 5.0 Sorbitan Sesquioleate 4.0 Polyoxy Ethylene (20 mol) Sorbitan monooleate 1.0 Propylene glycol 7.0 Ryobu 30% butanol extract 2.0 Tranexamic acid 1.0 Sodium bisulfite 0.01 Ethyl paraben 0.3 Perfume Appropriate amount Deionized water residue <Production method> Add propylene glycol to ion-exchanged water,
Heated and maintained at 70 ° C. (aqueous phase). The other components were mixed, melted by heating and kept at 70 ° C. (oil phase). The aqueous phase was gradually added while stirring the oil phase, uniformly emulsified with a homomixer, and then cooled to 30 ° C. while stirring well to obtain an emulsion.

[Example 5] Incorporation amount of gel-like cosmetic ingredients (% by weight) 95% ethyl alcohol 10.0 dipropylene glycol 15.0 polyoxyethylene (50 mol) oleyl alcohol ether 2.0 carboxyvinyl polymer 0 .05 (trade name: Carbopol 940, BFGoodrich Chemical company) Caustic soda 0.15 L-arginine 0.1 Akagi 30% ethanol extract 1.0 Sodium bisulfite 0.01 Ethylparaben 0.3 Perfume Appropriate amount Deionized water Residue <Production method> Carbopol 940 was uniformly dissolved in ion-exchanged water, while the red oak extract and polyoxyethylene (50 mol) oleyl alcohol ether were dissolved in 95% ethanol and added to the aqueous phase. Next, after adding other components, the mixture was neutralized with caustic soda and L-arginine to increase the viscosity to obtain a jelly.

Example 6 Packing Ingredients (Weight%) (A phase) Dipropylene glycol 5.0 Polyoxyethylene (60 mol) hydrogenated castor oil 5.0 (B phase) Olive oil 5.0 Tocopherol acetate 0.2 Ethylparaben 0.2 Fragrance 0.2 (Phase C) Sodium bisulfite 0.03 Polyvinyl alcohol 13.0 (Saponification degree 90, Polymerization degree 2,000) Ethyl alcohol 7.0 Kanrenboku 50% 1,3- BG extract 1.0 Purified water residue <Production method> A phase and B phase were each dissolved uniformly, and B phase was added to A phase.
The phases were added and solubilized. Next, after adding the phase C in which the extract of karenboku was dispersed, the mixture was filled to obtain a pack.

[0077]

According to the present invention, there is provided a new means capable of preventing or improving pigmentation, spots, freckles, various skin diseases accompanied by abnormal keratinization, rough skin, rough skin, skin aging, etc. after sunburn. .

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (Reference) A61K 7/00 A61K 7/00 RU 7/48 7/48 A61P 17/00 A61P 17/00 (72) Inventor Yumiko Suzuki 1050 Nippa-cho, Kohoku-ku, Yokohama-shi, Kanagawa Prefecture Inside Shiseido First Research Center Co., Ltd. (72) Inventor Taiichi Nakayama 1050 Nippa-cho, Kohoku-ku Yokohama-shi, Kanagawa Prefecture Shiseido First Research Center Co., Ltd. F term (reference) 4C083 AA082 AA111 AA112 AA122 AB032 AB152 AB352 AC012 AC022 AC072 AC092 AC102 AC122 AC182 AC242 AC352 AC422 AC442 AC482 AC542 AC582 AC622 AD042 AD092 AD112 AD512 AD662 CC05 CC07 DD31 DD41 EE12 EE16 4C088 AB44 MA

Claims (6)

[Claims] 1. A plant belonging to the genus Akagi (Bischofia B)
l.), one or more plants selected from the group consisting of plants belonging to the genus Iwanashi (Epigaea L.), plants belonging to the genus Kanrenboku (Camptotheca Decne.), and plants belonging to the genus Ryobu (Clethra L.) An external preparation for the skin, comprising an extract of the present invention as an active ingredient. 2. The plant belonging to the genus Akagi is a red oak (Bischofi).
a javanica Blume), the plant belonging to the genus Charnaceae is a char (Epigaea asiatica Maxim.), and the plant belonging to the genus Kanrenboku is a plant belonging to the genus Kanrenboku (Camptotheca acumi).
The external preparation for skin according to claim 1, wherein the plant belonging to the genus Ryobu is Ryobu (Clethra barbinervis Sieb.et Zucc.). 3. The external preparation for skin according to claim 1, which is an external preparation for whitening. 4. The external preparation for skin according to claim 1, which is an external preparation for improving rough skin. 5. The external preparation for skin according to claim 1, which is an external preparation for preventing skin aging. 6. The compounding amount of one or more plant extracts is 0.005 to 20.0% by weight of the agent as dry weight.
The external preparation for skin according to any one of claims 1 to 5, which is: