JPH01146811A - Cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic sufficiently showing emulsifying effects, moisture retaining function and emollient effects free from irritation to the skin, by blending a cosmetic base with a protein purified by treatment with a strongly basic anion exchange resin and by electrodialysis. CONSTITUTION:An aqueous solution of a vegetable protein, preferably soybean protein or one prepared by denaturing the soybean protein with an alcohol and/or partially hydrolyzing with an alkali or enzyme is passed through a strongly basic anion exchange resin of chlorine ion type, high-molecular anion in the aqueous solution is replaced with the chlorine ion and the aqueous solution is desalted by electrodialysis to give a purified vegetable protein having <=1wt.% ash content. A cosmetic base is blended with 0.05-10wt.% of the protein.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention relates to cosmetics containing vegetable proteins purified by treatment with a strong basic anion exchange resin and electrodialysis.

(b) Prior Art In recent years, consumers have become increasingly concerned about the safety of cosmetics, and for this reason, the synthetic raw materials that have traditionally been used are being replaced with natural raw materials.

From this point of view, cosmetics containing natural proteins in place of synthetic surfactants are also being used.

Furthermore, natural proteins also have a high moisturizing function and are suitable as raw materials for cosmetics.

An example of a protein incorporated into cosmetics is alcohol-denatured soybean protein partially hydrolyzed with a proteolytic enzyme. It is known that this decomposed protein is not only excellent as an emulsifier, but also that cosmetics containing it have high moisturizing and emollient effects, and also have an excellent feel on the skin. (Unexamined Japanese Patent Publication No. 58
-10512).

However, as an example of vegetable proteins that are conventionally used as raw materials for cosmetics, defatted soybeans are used as raw materials and are obtained through purification treatments such as alcohol washing and isoelectric precipitation, but impurities such as ash are completely removed. It is difficult to obtain products that have been removed, and some of the odor and color peculiar to soybeans cannot be removed.

Furthermore, in order to incorporate the soybean protein obtained in this way into cosmetics, it is desirable to hydrolyze it with acid or alkali.For this purpose, hydrochloric acid, sodium hydroxide, etc. are used, and at the end of the reaction, the pH is adjusted to neutral. It is necessary to return to Furthermore, when hydrolyzing with an enzyme, an acid or alkali is required to adjust the pH to the optimum pH of the enzyme used. Furthermore, it must be returned to neutrality after the enzymatic reaction is complete. Due to the above operations, the ash content is increased.

Colorless vegetable proteins suitable for formulation in cosmetics include
It also needs to be odorless (and free from other organic substances and inorganic substances (ash) that remain after ashing. However, in reality, as mentioned above, residual ash contained in the raw materials or , ash is included in the product due to operational requirements during hydrolysis.

Odor 2 It goes without saying that coloration is undesirable for raw materials for cosmetics, but the presence of ash not only has a negative effect on the emulsion system, but even more unfavorably, it can cause skin irritation such as itching and red spots. , or encouraged.

(C) Problems to be Solved by the Invention As mentioned above, the present invention aims to provide a cosmetic containing a vegetable protein that does not irritate the skin and has a large emulsifying effect. For this purpose, as mentioned above, it is necessary to remove ash from vegetable proteins.
An example of using an ion exchange resin for the purpose of neutralization (Japanese Patent Application Laid-open No. 1983-
No. 101449), but in this case, only an acid or alkali is not used for neutralization after hydrolysis, and there is a problem that the ash derived from the raw material remains without being desalted. In addition, JP-A-61-183298 discloses a method for desalting the ash content in polypeptide derived from animal protein by electrodialysis, but in the case of a hydrolyzed vegetable protein solution, direct electrodialysis is used. When desalting was attempted, the polymeric anion component in the vegetable protein contaminated the membrane, making it virtually impossible to desalt.

Therefore, up until now, cosmetics containing vegetable proteins with a high ash content have been used as is, but it is necessary to lower the blending ratio of cosmetics to a concentration that does not have the effect of ash irritating the skin. was there.

For this reason, it could not be said that the emulsifying effect, moisturizing function, and emollient effect, which are characteristics of vegetable proteins, are fully exhibited.

(d) Means for Solving the Problems The present invention relates to a cosmetic composition characterized by containing a strongly basic anion exchange resin and a vegetable protein purified by electrodialysis. In other words, by passing an aqueous solution of vegetable protein for cosmetics through a chloride ion type of strongly basic anion exchange resin, the anion component with a large molecular weight in the aqueous solution of vegetable protein is replaced with chloride ions, and electrodialysis is performed. The present inventors have discovered that ash desalination by this method can be carried out satisfactorily, and that the ash content is 1% or less based on the weight of vegetable protein, and the present invention has been completed. This has made it possible to increase the blending ratio of vegetable proteins in cosmetics, making it possible to produce cosmetics that fully utilize their properties.

The present invention will be explained in further detail.

The vegetable proteins used in the present invention include soybean protein,
Examples include corn protein and sesame protein, but soybean protein is industrially advantageous.

As the soybean protein, those described below can be used. That is, defatted soybeans produced from the soybean oil extraction process are used as raw materials, and concentrated soybean proteins, isolated soybean proteins, etc. obtained from the defatted soybeans can be used.

Furthermore, it is preferable to wash these proteins with alcohol, as part of the color and odor can be removed. Alcohol cleaning may be performed by a known method.

Although the above-mentioned vegetable protein or the vegetable protein obtained by washing it with alcohol can be purified as it is by using an ion exchange resin and electrodialysis, it is preferable to hydrolyze the protein with an acid/alkali or a proteolytic enzyme. A known method can be used for this. For example, when hydrolyzing with acids, hydrochloric acid, sulfuric acid, acetic acid, etc.
% (w/w) at a pH of 3 or below and at a temperature of 40%.
It is carried out for 1 to 24 hours at ~100°C. When using alkali, add sodium hydroxide, potassium hydroxide, etc. from 1 to 30
% (W/W) and at a temperature of 10 to 100°C.
Allow to react for 24 hours. Furthermore, when using proteolytic enzymes, use pepsin, biobrase (Nagao Sangyo), papain, trypsin alcalase, etc., and adjust the pH using an appropriate reagent between 1 and 1 of the optimum pH of each enzyme, and at a temperature of 30 to Process between 1 and 24 hours at 50°C.

Tsurupi 2 is a soybean protein that has been treated in this way.
000 (Nissin Oil ■Art product name), Hydrosoy 20
00SF (trade name manufactured by Croda Japan) etc. are commercially available. These hydrolyzed vegetable proteins usually contain 5
It contains ~15% ash, and if used as a cosmetic as it is, it will irritate the skin and have a negative effect on the emulsification effect.

Therefore, in the present invention, these vegetable proteins purified using a chloride ion type strongly basic anion exchange resin and an electrodialysis method are employed. As a purification method, the above-mentioned vegetable protein or a vegetable protein solution obtained by hydrolyzing the same is brought to a protein concentration of 15% or less, preferably 5 to 10%, and a strongly basic anion exchange resin, such as Diaion 5AI, is used.
Gel type, preferably Diaion P
The liquid is passed through a porous type such as A30B, PA312 (all product names manufactured by Mitsubishi Kasokoga Co., Ltd.), Amberly) IR^-900 (trade name manufactured by Rohm and Haas Co., Ltd.). This strongly basic anion exchange resin is used in the sulfate ion type, nitrate ion type, preferably chloride ion type, in order to replace the anion component in the vegetable protein or hydrolyzed vegetable protein solution with the anion of an inorganic acid. It will be done.

As mentioned above, the anion component is replaced with chlorine ion,
Vegetable protein or hydrolyzed vegetable protein solutions whose ash has become inorganic cation chloride can be desalted by ion exchange membrane electrodialysis, and the ash content relative to the weight of vegetable protein or hydrolyzed vegetable protein can be desalinated by ion exchange membrane electrodialysis. can be reduced to 1% or less. The electrodialysis device used here may be a commonly used filter press type device in which cation exchange membranes and anion exchange membranes are arranged alternately to create demineralization chambers and concentration chambers alternately, and Ion exchange membranes include cation exchange membranes for general use such as CM-1 and CM-2 (both manufactured by Tokuyama Soda ■) and CMV (trade name manufactured by Asahi Glass ■), as well as AM-1 and AM-2. Anion exchange membranes for general use such as AMV (trade name of Asahi Glass Co., Ltd.) and the like may be used, but special membranes such as fluorine-based membranes are also suitable.

By the above method, the desalted and purified vegetable protein or hydrolyzed vegetable protein has an ash content of 1% based on the protein weight.
The color was good, and there was no odor or taste peculiar to soybeans.

In addition, the aqueous solution after electrodialysis may be used as it is,
Alternatively, it can also be used as a powder after drying.

The above purified vegetable proteins can be used to replace all or part of the surfactants conventionally used as emulsifiers, moisturizers, and emollients in cosmetics, and their blending amounts are partially specified. Not possible, but generally 0.05-10
Weight%.

Cosmetics that can be blended are not limited to the examples and blending examples described below, and include creams such as cold cream and vanishing cream, milky lotions, lotions, bank agents, shampoos, hair treatments, and the like.

(e) Effects of the Invention The cosmetic containing purified soybean protein obtained by the present invention has the following characteristics.

(1) It feels smooth and supple to the skin, etc.

(2) It has high moisturizing and emollient properties.

(3) It has good stability and does not cause any change in the product during storage.

(4) It is extremely safe and does not irritate the skin.

(f) Examples Next, the present invention will be explained in detail with reference to Examples.

However, in the examples, "%" is used unless otherwise specified.
% by weight. Further, the present invention is not limited to the following examples unless it exceeds the gist thereof.

Example 1 (1) 21 grams of Diaion PA312, a chloride ion type, was added to 6 It of a 10% aqueous solution of Tsurupi 2000 (ash content 8.7%, enzymatically decomposed soybean protein: Nisshin Oil Co., Ltd. side type) and shaken at room temperature for 2 hours. After stirring, suction filtration was performed using filter paper to obtain 5.71 ml of ion exchange resin treated liquid. The protein concentration of the treatment solution is 8
．． It was 5%. The analysis results of ash content are shown in Table 1.

(2) 21 of the above ion exchange resin treated solutions were desalted by electrodialysis under the following conditions to obtain 1.951 treated solutions. The protein concentration of this treatment solution was 8.4%. The analytical values for ash content are shown in Table 1.

■Test equipment Du-Ob electrodialysis device manufactured by Asahi Glass ■Ion exchange membrane used Cation exchange membrane CMV (manufactured by Asahi Glass ■) Anion exchange membrane AMV (manufactured by Asahi Glass ■) ■Effective membrane area 1.72 (dm)" x 9 pairs = 0.1
55 m'' ■Electricity time 60 minutes ■Voltage 12 Volt - Table 1 Umbrella ash content indicates the ratio of the residue after ashing to protein.

The zero umbrella ash composition is the result of quantifying the content of each component in the protein aqueous solution.

Example 2 A cosmetic containing the purified hydrolyzed soybean protein obtained in Example 1 was produced.

Formulation example-1 hand cream Component (2) was added to purified water, dissolved, and heated to 70°C.

On the other hand, after mixing the components (1) and dissolving them by heating at 70°C,
Add to the former. After uniformly emulsifying at 70°C, the mixture was cooled to 30°C while stirring.

The hand cream thus obtained was stored at 50° C. for 6 months, but no change was observed.

In addition, it does not feel sticky and feels very supple.
Furthermore, no irritation to the skin was observed.

Formulation Example-2 Lotion Ethanol 15.0 Add each component of (1) to purified water and dissolve. On the other hand, dissolve each component (2) in ethanol and mix the two.

A product was obtained by stirring.

The lotion thus obtained was stored at 50°C for 6 months, but no changes were observed. In addition, the area of use had a very smooth and supple feel. Furthermore, no irritation to the skin was observed.

Formulation example-3 Emollient cream monostearate 3.0 in purified water (2)
Add each component and heat to 70°C to dissolve. Then (1
) were added and heated to 70°C to uniformly emulsify.
Cool to 0°C.

50% of the emollient lotion thus obtained
Although it was stored at ℃ for 6 months, no change was observed. In addition, the application area was extremely smooth and had an excellent moist feeling. Furthermore, no skin irritation was observed.

Formulation example-4 Hair rinse Stearyldimethylbenzylammonium chloride 2.0 Stearyltrimethylbenzylammonium chloride 2.0 Stearyl alcohol 0.5 Propylene glycol 1.0 Purified hydrolyzed soybean protein
2.0 glycerin monostearate
1.5 Fragrance and preservative Appropriate amount of purified water The remaining components were mixed and dissolved in purified water to obtain a product.

The thus obtained hair rinse was stored at 50°C for 6 months, but no changes were observed. Also, after use,
My hair became moist and supple. Furthermore, no irritation to the scalp was observed.

Patent applicant: Nisshin Oil Co., Ltd. Patent applicant: Nippon Rensui Co., Ltd.

Claims (3)

[Claims] (1) A cosmetic containing a strongly basic anion exchange resin and a vegetable protein purified by electrodialysis. (2) The cosmetic according to claim 1, wherein the purified vegetable protein has an ash content of 1% by weight or less. (3) Claim 1 in which the vegetable protein is soybean protein or a product obtained by subjecting it to alcohol denaturation treatment and/or partial hydrolysis treatment with acid, alkali, or enzyme.
Cosmetics listed in section.