JPH01249709A - Cosmetic containing hen's egg polypeptide derivative - Google Patents

Abstract

PURPOSE:To obtain a cosmetic, containing a quaternary trimethylammonium derivative of a polypeptide prepared by hydrolyzing proteins of hen's egg and having great effects on improvement in suppleness, gloss and smoothness of the hair and emollient properties even on the skin. CONSTITUTION:A cosmetic obtained by blending a trimethylammonium derivative of a polypeptide prepared by hydrolyzing proteins of hen's eggs with an enzyme, acid or alkali and reacting the resultant water-soluble polypeptide having 500-20000, especially 1000-10000 average molecular weight with 3-chloro-2- hydroxypropyltrimethylammonium salt under alkaline conditions of about pH 9-12 in an amount of about 0.2-15% expressed in terms of solids with other ingredients of the cosmetic. The above-mentioned compound of the resultant trimethylammonium derivative has no unpleasant animal odor in spite of slight odor which is not unpleasant but unique to the egg. The compound especially made of only egg white as a raw material is light colored and excellent.

Description

[Detailed description of the invention] (Industrial application field) The present invention relates to a new cosmetic.

(Prior art and its drawbacks) Quaternary ammonium derivatives are used in cosmetics, especially hair rinses, and have excellent adsorption to hair, improving hair flexibility, luster, and
It has the effect of improving smoothness.

7 Among them, stearyldimethylbenzylammonium chloride, stearyltrimethylammonium chloride, etc. have been used for a long time.

Recently, in order to further increase the effect, it has been proposed that hydrolysates of mammalian proteins such as collagen and keratin are converted into quaternary ammonium.

However, polypeptides obtained by hydrolyzing mammalian proteins such as collagen and keratin, as well as their quaternary ammonium derivatives, are not free from a peculiar animal odor, although the degree of This has become a problem amid the trend to stop or reduce the amount of fragrance added to cosmetics.

(Objective of the invention) To provide a cosmetic containing a quaternary ammonium derivative that is highly effective in improving the flexibility, gloss, and smoothness of hair, has emollient properties for the skin, and has no peculiar animal odor. For a different purpose.

(Structure and Effects of the Invention) Conventionally, raw materials for polypeptides that can be incorporated into cosmetics include mammalian collagen, elastin, keratin,
These were listed in the General-purpose Cosmetic Ingredients and were widely known in the industry.

Therefore, these quaternary ammonium derivatives were also synthesized,
Already published.

However, the problem is that all of these have their own unique animal odor.

This animal odor does not seem to bother Westerners who eat a lot of meat, but many Japanese find it unpleasant.

In order to eliminate such drawbacks, the present inventors conducted various studies and found that the use of bird egg protein as a raw material can greatly improve the results.

The configuration of the present invention will be explained in detail below.

First, chicken eggs are currently the most convenient to prepare by hand, and thus meet the purpose of the present invention. But the duck,
You can also use quail eggs.

Rabbits have egg whites and egg yolks, and the egg whites account for 56-5% of the weight of the whole egg.
Of this, about 90% is water and about 10% is protein, and apart from a trace amount of carbohydrates, it contains almost no other organic components.Therefore, it is an excellent raw material for the present invention. .

On the other hand, about 50% of egg yolk is solid, of which 52% is fat and about 16% is protein. However, those obtained by removing the fat and oil with an organic solvent such as ether can be used in the present invention. Alternatively, whole eggs can be used as they are.

Next is hydrolysis, which can be roughly divided into three methods.

That is, hydrolysis by enzymes, acids, and alkalis.

In the case of enzymatic hydrolysis, the enzymes that can be used are those belonging to proteases, such as pepsin, papain, trypsin, or those of bacterial origin.

However, during hydrolysis, it is necessary to select p) (and temperature conditions suitable for the enzyme).

In the case of acid decomposition, hydrochloric acid, sulfuric acid, phosphoric acid, nitric acid, etc. can be used, and in the case of alkaline decomposition, sodium hydroxide, potassium hydroxide, sodium carbonate, etc. can be used.

The hydrolysis of the present invention may be carried out by any of them.

The average molecular weight of polypeptide after hydrolysis is 500-2
0, OOO is fine, but 1,000 to 10,00
It is more preferable if it is 0.

Furthermore, it is unavoidable that a certain amount of substances that have been broken down to unit amino acids are mixed together.

If the average molecular weight is too small, the effect when blended into cosmetics will be small, and if it is too large, the water solubility will be poor.

Prior to converting the aqueous hydrolyzate solution thus prepared into quaternary ammonium, if necessary, it is subjected to deionization treatment using an ion exchange resin, concentration, dilution, or decolorization and deodorization treatment using activated carbon or the like.

Quaternary ammonium formation is performed by reacting a 3-chloro-2-hydroxypropyltrimethylammonium group with a polypeptide.

The salt is not limited to hydrochloride, but aqueous solutions of hydrochloride are commercially available and can be suitably used.

The reaction is a reaction in which hydrogen chloride is generated from the hydrogen atom of the amino group of the polypeptide and the chlorine atom at the 3-position of the 3-chloro-2-hydroxypropyltrimethylammonium salt.
The process proceeds by gradually dropping the 3-chloro-2-hydroxypropyldimethylammonium group into a 10-40% aqueous solution of the polypeptide while stirring at room temperature to 50°C.

At this time, the reaction solution must be alkaline with a pH of about 9 to 12.

To make it alkaline, it is necessary to add an alkali such as sodium hydroxide or potassium hydroxide.

If the solution is left for about 24 hours after the completion of the dropwise addition, the reaction will proceed almost completely.

After the reaction, the solution is returned to approximately neutrality using an acid such as hydrochloric acid, sulfuric acid, or phosphoric acid, or using an ion exchange resin.

This liquid may be used as a raw material for cosmetics by adding a preservative or the like, or it can be dried and powdered by a method such as freeze-drying to be used as a raw material for cosmetics.

When the quaternary trimethylammonium derivative of the avian egg protein hydrolyzate produced in this way is incorporated into a cosmetic, the cosmetic may be a hair cosmetic such as a hair rinse or a cold perm solution, or , skin lotions, cream emulsions, and other skin products.

The blending amount is preferably about 0.2 to 15% in terms of solid content.

However, when other ingredients include anionic surfactants, water-insoluble precipitates may form, so care must be taken when formulating the formulation.

When the quaternary ammonium derivative of the avian egg protein hydrolyzate of the present invention is incorporated into hair cosmetics such as shampoos, hair rinses, hair conditioners, and permanent wave liquids, it improves the flexibility, smoothness, luster, and combability of the hair after use. It has the effect of improving the street. When added to skin cosmetics such as creams, milky lotions, and lotions, it imparts emollient properties and smoothness to the skin. In addition, it has a slight unpleasant odor unique to eggs, but no unpleasant animal odor. Also, when using only egg white as the raw material, the color is lighter and better than when using other raw materials.

(Example 1) 100 parts of fresh chicken egg white was dispersed in 200 parts of water, and the pH
was adjusted to 7.0.

1 part of cysteine and 1 part of papain were added to this and hydrolyzed at 40°C for 8 hours.

During this time, the pH decreased, so the pH was adjusted to 7 with monoarsenium hydroxide every 2 hours.

After hydrolysis, the mixture was heated at 70°C for 30 minutes to lose enzyme activity.

After cooling, it was filtered and the filtrate was concentrated at 70°C under reduced pressure.

At this time, a slight precipitate formed, so it was filtered again.

The solid content concentration after refiltration was 35%.

100 parts of this liquid was placed in a reaction vessel and while stirring at 40°C, 12 parts of a 50% aqueous solution of 3-chloro-2-hydroxypropyltrimethylammonium chloride was added dropwise over 20 minutes, and then an aqueous sodium hydroxide solution was added dropwise to the solution. The pl after the reaction was adjusted to 10.0 to 11.0.

Thereafter, stirring was continued at 40° C. for 6 hours, during which time the pH was adjusted.

Thereafter, stirring was stopped and the mixture was left at room temperature for 2 days.

The final pl (was 10.3).

Hydrochloric acid and water were added to this liquid and filtered, and the pH was adjusted to 6.5 and the solid content concentration to be 25%.

The color tone of the resulting liquid A was made transparent using Gardner I.

(Example 2) 100 parts of dried food-grade whole eggs (mixture of egg yolk and egg white) were crushed, washed with ethanol, dried, and then 500 parts of purified water and 20 parts of concentrated hydrochloric acid (35% concentration) were added and stirred. while 70℃
Hydrolysis was carried out for 5 hours. After cooling, the mixture was neutralized with a basic anion exchange resin, concentrated under reduced pressure at 70°C, and then filtered. The resulting filtrate had a solids content of 38%. This was converted into quaternary ammonium in the same manner as in Example 1 to obtain a liquid with a solid content of 25%.

This liquid B had a color tone of Gardner 3, and although the odor was slightly inferior to that of liquid A in Example 1, it was still usable.The average molecular weight of the polypeptide derivative in liquid B was approximately 15.
It was 00.

(Example 3) A collagen hydrolyzate with an average molecular weight of f14,000 was made into a quaternary ammonium derivative using the same method as in Example 1. Liquid C (
Hair rinses (cream-like) a, b, and c containing liquids A and B of Examples 1 and 2 (solid content 25%), and hair rinses d without the mixture were experimentally produced. The prescription is shown in Table 1.

Table 1 Hair rinses a, b, c, and d were prepared as 20% aqueous solutions, and a bundle of hair was immersed in each at 35° C. for 5 minutes, then washed with water and air-dried for comparison. However, the odor test was conducted using the immersion liquid itself.

Δ Slightly Poor X Poor (Example 4) Three types of skin creams were made using the following formulations: two types containing quaternized peptides and one type in which water was added instead.

Liquid J Pilafine
Rough-in to 20% solid (melting point 65℃)
5 cetylfur]-ru
2.
5H00RiokinEthitonSte7SnFull]-le
2 Above liquid A or liquid B8 Purified water 58 Are there any products using liquid A? The a BO product was also superior in spreadability and smoothness during use, as well as its ability to prevent skin dryness (moist feeling), compared to the product without the addition of quaternized peptide.

There was also no peculiar animal odor.

Claims (1)

[Claims] (1) Contains a polypeptide quaternary trimethylammonium derivative obtained by reacting a 3-chloro-2-hydroxypropyltrimethylammonium group with an aqueous solution of a water-soluble polypeptide obtained by hydrolyzing bird egg protein. Cosmetics featuring: