JP3403743B2 - Skin cosmetics - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin cosmetic, and more specifically, whey mineral containing a non-adsorbed fraction obtained by treating whey with an ion-exchange resin as a main component, and / or the whey mineral. The present invention relates to a skin cosmetic containing a treated product.

[0002] The object of the present invention is NMF (Natural Moist) which is a natural skin moisturizing ingredient contained in milk.
Urising Factor), a tyrosinase inhibitor, a substance having no antigenicity, and a physiologically active substance contained in the polymer fraction are effectively used.
It is an object of the present invention to provide a safe skin cosmetic having an excellent effect and free from cosmetic problems.

[0003]

2. Description of the Prior Art Raw milk, particularly milk, has been handed down by dairy farmers etc. for its effectiveness such as preventing skin roughness, fine wrinkles, and spots, and smoothing the skin.
It was used for private consumption as a bath agent typified by a milk bath. However, milk contains a large amount of high-molecular proteins, and when heated or acidified, it aggregates and becomes insoluble, which is a problem in use. In milk, casein, which is considered to be a causative agent of allergies, β
-A high molecular protein such as lactoglobulin or α-lactalbumin is contained, and it is not preferable to use it as it is from the viewpoint of safety to the skin.

From this point of view, it has been proposed to remove the above-mentioned harmful substances by ultrafiltration of raw milk, skim milk, whey and the like, and use a filtrate containing only a low molecular fraction as a cosmetic. However, the fractions removed by ultrafiltration contain components useful as cosmetic raw materials such as glycoproteins and nucleic acid derivatives, and the low molecular weight fractions are also affected by heating and changes in pH. Whey mineral purified by only the ultrafiltration method is not preferable as a cosmetic ingredient because it contains a component that causes reversible polymerization.

[0005]

SUMMARY OF THE INVENTION In view of the above-mentioned state of the art, the present invention has been completed by intensive research for the purpose of separating a component safer than milk and having a higher physiological activity and blending it into cosmetics. Is. Its characteristic is to obtain a new type of whey mineral which has a higher content of physiologically active substances than known whey minerals by further removing lactose from the non-adsorbed fraction obtained by treating whey with an ion exchange resin. It is included in skin cosmetics.

The above-mentioned new type of whey mineral contains glycomacropeptide (GM) as a physiologically active ingredient.
P) is contained in about 2 times the amount of whey in terms of solid content, and sialic acid and its derivatives are contained in about 4 times the amount of whey.

On the other hand, a patent has already been filed for cosmetics and the like using whey minerals, and JP-A-63-17981
No. 8 and Japanese Patent Application Laid-Open No. 1-254610 are published. Of these, the former is one in which a whey mineral obtained by an ultrafiltration method is mixed in a bath agent, and it has been confirmed that the above-mentioned physiologically active substance is very small. The latter relates to a cosmetic composition comprising a composition obtained by removing a high molecular weight substance having a molecular weight of 13,000 or more from milk.

However, it is known that many physiologically active substances and derivatives of physiologically active substances are high molecular substances having a molecular weight of 13,000 or more. For example, lactoferrin, which has been reported to have an antibacterial effect, has a molecular weight of about 80,000.
Then, there is a DNA having a molecular weight of 1,000,000 or more, which is expected to have an ultraviolet protection effect. Therefore, as a cosmetic, as a compounding component thereof, the effect, as the whey mineral most expected to be effective, the content of the physiologically active component is high as in the present invention, and α-lactalbumin,
It can be said that the allergenic components such as β-lactoglobulin are removed.

The whey mineral used in the present invention is the non-adsorbed fraction itself obtained by treating whey with an ion exchange resin, and / or a treated product thereof. The treated products include those obtained by partially or completely removing lactose from the non-adsorbed fraction, those obtained by removing high molecular weight substances having a molecular weight of 100,000 or more, and other treated products of the non-adsorbed fraction. It

The above-mentioned whey mineral used in the present invention is prepared, for example, as follows.

Cheese whey and other whey are treated with an ion exchange resin after adjusting the pH if necessary.
As the ion exchange resin, both a cation exchange resin and an anion exchange resin can be used, but a type having a protein selective adsorption property is particularly effective. Specifically, those having a sulfonic acid group, sulfopropyl group, carboxylic acid group and carboxymethyl group are cellulosic and silica type ones having a quaternary ammonium group, a primary to tertiary amine and the same amide. It can be used regardless of. Usually, commercially available products can be appropriately used, but carboxymethyl cellulose type and sulfopropyl cellulose type are preferable.

The treated product of the non-adsorbed fraction used in the present invention is prepared, for example, as follows.

The non-adsorbed fraction obtained by the above-mentioned treatment with an ion exchange resin is concentrated and cooled to crystallize lactose and remove it, or by other known lactose removing means to partially remove lactose. Alternatively, remove all to obtain whey minerals. The obtained whey mineral may be kept in a liquid state as described above, but it may be dried by a commonly used drying means such as spray drying or freeze drying after being stored and handled.

Further, although sterilized as needed, workability,
Considering economical efficiency and sterilization effect, it is desirable to use a plate sterilizer for instant sterilization. Of course, any known means can be adopted. If it is desired to avoid heat denaturation as much as possible, bacteria should be filtered using a microfiltration membrane with a pore size of 0.2μ. When a transparent solution is required, it can be subjected to pressure filtration using a microfiltration membrane or an ultrafiltration membrane after cleaning to increase the transparency. Known forms and materials can be used for the film. When increasing the transparency by microfiltration, it is desirable that the pore size be 0.2 μm or less. Further, when using the ultrafiltration method, it is necessary to transfer the physiologically active substance to the permeate side. Therefore, a membrane having a cut-off molecular weight of 100,000 or more is used and a membrane capable of withstanding a filtration pressure of 10 kg / cm ^2. Is required.

The components contained in the whey mineral according to the present invention thus obtained are as follows.

(1) General analysis (analysis example)

[0017]

[Table 1]

(2) Mainly contained minerals (ash) Sodium, potassium, chlorine, phosphorus, calcium and magnesium.

(3) Minor amount of minerals (ash) Iodine, zinc, iron, copper, manganese.

(4) Mainly contained amino acids serine, aspartic acid, threonine, glutamic acid,
Brolin, glycine, alanine, valine, isoleucine, leucine, phenylalanine, lysine, histidine, arginine, methionine.

(5) Amino acid contained in a trace amount Tryptophan, cystine, tyrosine.

(6) Nucleic acid-related substances mainly contained in DNA, RNA, uridine, 5'-uridylic acid, 5'-
Cytidylic acid, orotic acid, adenine derivatives.

(7) Non-protein nitrogen compounds other than the above mainly contained urea, ammonia, phosphoserine, ortinin, citrulline, glucosamine, creatine, and creatinine.

(8) Physiologically active substances mainly contained sialic acid and its derivatives, glycomacropeptides, various vitamins.

The nitrogen-containing component content varies depending on the level of clarification of the aqueous solution, but considering the content of the physiologically active substance, the content of crude protein (N × 6.38) must be in the range of 3 to 15%.

The skin cosmetics according to the present invention are lotions, creams, emulsions, packs and all other skin cosmetics, and are active ingredients such as bases and auxiliaries commonly used in skin cosmetics according to the conventional method. It may be produced by mixing an appropriate amount of (whey mineral described above), for example, 0.001 to 15%, preferably 0.01 to 10%. Since the active ingredient is derived from foods, it has no inherent toxicity problem, and since it is externally applied to the skin, no safety problem is observed.

To prepare a lotion among the skin cosmetics according to the present invention, for example, a moisturizing agent such as glycerin, propylene glycol, citric acid, a pH adjusting agent and the like are dissolved in purified water to prepare a surfactant, In the production of a general lotion in which a preservative, a fragrance, etc. are dissolved in alcohol and both are mixed to solubilize, 0.01 to 10 of the active ingredient of the present invention is added to the water-soluble part.
Add lotion so that the amount becomes%.

To prepare a cream, for example, a hydrophilic component such as glycerin or sorbit is added to purified water to form an aqueous phase, and beeswax, paraffin, microcrystalline wax, ceresin, higher fatty acid, hydrogenated oil is used. A solid oil component such as, and liquid oil components such as squalane, liquid paraffin, and various ester oils are mixed with oil components such as preservatives and surfactants to prepare an oil phase part, and the water phase thus obtained Part and oil phase part are respectively heated, mixed, stirred, and emulsified to produce a general cream, and 0.01 to 10% of the active ingredient is added to the water phase part.
Add to make cream.

To prepare an emulsion, for example, a moisturizing agent such as glycerin or 1,3-butylene glycol is added to purified water and mixed by heating to form an aqueous phase. Solid oil components such as beeswax and paraffin, vaseline, Semi-solid oil such as lanolin,
Liquid ingredients such as squalane, liquid paraffin and various ester oils are prepared by adding oily ingredients such as preservatives and surfactants to make an oil phase part, and the oil phase part is added to the water phase part for emulsification. In the production of a general emulsion containing a carboxyvinyl polymer, a thickener such as carboxymethyl cellulose, to prepare an emulsion, the active ingredient of the present invention is added to the aqueous phase portion in an amount of 0.0
Add 1 to 10% to make an emulsion.

To prepare the pack, for example, a humectant such as glycerin and sorbit, a filming agent such as polyvinyl alcohol and polyvinylpyrrolidone are added to purified water and swelled, and if necessary, kaolin, talc, and oxidation are added. In the production of a general pack in which powder such as titanium is added and ethanol in which a flavoring agent, a preservative and the like are dissolved is kneaded until a paste is formed, the active ingredient of the present invention is added to 0.0
Add 1 to 10% to make a pack.

The present invention will be further described below with reference to test examples and examples.

[0032]

Test Example 1 Cheese whey was adjusted to pH 3 to 4, preferably about 3.5, and then passed through a column packed with an ion exchange resin to obtain a non-adsorbed fraction. As the ion exchange resin, an indeion S type cation exchange resin (trade name of Phoenix Chemical Co. (New Zealand)) was used. The non-adsorbed fraction that passed through the resin column was purified using a clarifier, filtered using a microfiltration membrane with a pore size of 0.2μ, concentrated under reduced pressure and cooled to crystallize lactose,
After removing this, the residual liquid was spray-dried to produce the whey mineral according to the present invention (this sample is referred to as whey mineral I).

On the other hand, as a control product, the same cheese whey as the starting material of the whey mineral according to the present invention was ultrafiltered, and this permeate was produced according to the method for producing whey mineral according to the present invention (this The sample is whey mineral II). The composition comparison of each prepared is shown in Table 2 below.

[0034]

[Table 2]

[0035]

[Test Example 2] The whey minerals I and II shown in Test Example 1 and the 50% ethanol fraction of whey mineral I were added to a 5 mM tyrosine solution so that the final concentration was 2.5%. Tyrosinase 250 units / ml was added and reacted at 37 ° C. for 10 minutes, and then 30% acetic acid was added to stop the reaction. The amount of quinone produced was measured by measuring the 660 nm absorption value of this solution, and the tyrosinase inhibitory activity was examined. The results are shown in Fig. 1.

As a result, the whey mineral of the present invention had a higher tyrosinase inhibitory activity than the control product. Further, the tyrosinase inhibitory activity of the fraction of this whey mineral is lower than that of the unfractionated product, suggesting that the tyrosinase inhibitory activity is enhanced by the synergistic action of various components contained in the whey mineral of the present invention. It was

[0037]

Example 1 Based on the formulation shown in Table 3 below, the whey mineral I formulation according to the present invention obtained in Test Example 1 (compounding ratio: 0%,
Three types of 0.1% and 0.2%) cosmetic creams were prepared.

[0038]

[Table 3]

The effect of the cosmetic cream according to the present invention was confirmed as follows.

The inner part of the forearm of the panelist was washed with a face-washing foam and lightly wiped off with water, and the above-mentioned cosmetic cream was washed with about 0.
1 g was applied and the water content (Wo) was measured. The application part was left open, and each water content (Wt) was measured after 10, 30, 60, 120, and 180 minutes.

The residual water content was 1 immediately after the application of the cosmetic cream.
It was calculated from the following equation as 00%. Moisture residual rate = Wt / Wo × 100

The results are shown in FIG. As is clear from the results, the higher the amount of whey mineral added, the higher the residual water content and the greater the moisturizing effect.

[0043]

Example 2 Using the cosmetic cream prepared in Example 1, an occlusive patch test was conducted on 50 panelists. Two types of observation were conducted, 24 hours and 48 hours after application.
As a result, the whey mineral 0.1%, 0.2% blended product,
Erythema was not observed as in the whey mineral-free product, and it was judged that the addition of whey mineral had little effect on the skin.

[0044]

Example 3 Using the whey mineral I obtained in Test Example 1,
An emulsion was prepared according to Table 4 below.

[0045]

[Table 4]

[0046]

EFFECTS OF THE INVENTION The skin cosmetic composition of the present invention is safe for the skin and has an excellent effect on skin roughness, spots, etc., and can keep the skin moisturized and healthy. It can be effectively used as a component of cosmetics. In addition, there is no restriction | limiting in particular, when mix | blending these with cosmetics, it can mix | blend arbitrarily with a normal skin cosmetics base.

[Brief description of drawings]

FIG. 1 is a graph showing the amount of quinone produced in each sample in Test Example 2 as an absorption value at 660 nm.

FIG. 2 is a graph showing the relationship between the residual water content on the skin and time.

─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kenichi Kakimoto 1565, Sunagoda, Kawagoe City, Saitama Prefecture 30 (72) Inventor Koji Takano 4-33-22 Midorimachi, Tokorozawa City, Saitama Prefecture (72) Inventor Yu Kuwata 9-56, Yamaguchi 963, Tokorozawa, Saitama Prefecture (56) References JP-A-4-187619 (JP, A) JP-A-3-206027 (JP, A) JP-A-3-20206 (JP, A) Kaihei 1-254610 (JP, A) JP 1-163110 (JP, A) JP 63-179818 (JP, A) JP 63-39545 (JP, A) (58) Fields investigated ( Int.Cl. ⁷ , DB name) A61K ^7/ 00-7/50

Claims (4)

(57) [Claims] 1. A cellulose-based or silica-based ion exchange resin in which whey has a functional group selected from the group consisting of a sulfonic acid group, a sulfopropyl group, a carboxylic acid group, a quaternary ammonium group, a primary amine, a tertiary amine and the same amide. Whey mineral containing as a main component a non-adsorbed fraction obtained by adsorbing allergenic proteins such as α-lactalbumin and β-lactoglobulin by treatment with whey mineral and / or a processed product thereof as an active ingredient Skin cosmetics characterized by: 2. Whey adjusted to pH 4.0 or less is treated with a cellulose-based or silica-based ion-exchange resin having a sulfopropyl group to obtain α-lactalbumin and β-.
Whey mineral mainly composed of non-adsorbed fraction obtained by adsorbing allergenic protein such as lactoglobulin and /
Alternatively, a skin cosmetic comprising the treated product as an active ingredient. 3. (A) Whey is a sulfonic acid group, a sulfopropyl group, a carboxylic acid group, a quaternary ammonium group,
Α- by treatment with a cellulose-based or silica-based ion exchange resin having a functional group of either tertiary amine or amide
Whey mineral containing as a main component a non-adsorbed fraction obtained by adsorbing allergen-causing proteins such as lactalbumin and β-lactoglobulin, (B) Obtained by partially or completely de-lactose the whey mineral (A) Whey mineral, and (C) the above whey mineral (A) has a molecular weight of 100,00
A skin cosmetic comprising as an active ingredient at least one selected from the group consisting of whey mineral obtained by removing 0 or more polymer substances. 4. (A) Whey adjusted to pH 4.0 or less is treated with a cellulose- or silica-based ion exchange resin having a sulfopropyl group to treat allergenic proteins such as α-lactalbumin and β-lactoglobulin. Whey mineral containing a non-adsorbed fraction as a main component obtained by adsorption, (B) Whey mineral obtained by partially or wholly lactose-eliminating the whey mineral (A), and (C) the milk Molecular weight 100,00 from pure mineral (A)
A skin cosmetic comprising as an active ingredient at least one selected from the group consisting of whey mineral obtained by removing 0 or more polymer substances.