JP7845176B2 - Ｌ－グルタミン酸オキシダーゼ変異体 - Google Patents

Ｌ－グルタミン酸オキシダーゼ変異体

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Publication number
JP7845176B2
JP7845176B2 JP2022510522A JP2022510522A JP7845176B2 JP 7845176 B2 JP7845176 B2 JP 7845176B2 JP 2022510522 A JP2022510522 A JP 2022510522A JP 2022510522 A JP2022510522 A JP 2022510522A JP 7845176 B2 JP7845176 B2 JP 7845176B2
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JP
Japan
Prior art keywords
region
glutamate
amino acid
glutamate oxidase
seq
Prior art date
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JP2022510522A
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English (en)
Japanese (ja)
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JPWO2021193598A1 (https=
Inventor
一敏 ▲高▼橋
浩輝 山口
宇乃 田上
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
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Ajinomoto Co Inc
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Publication of JPWO2021193598A1 publication Critical patent/JPWO2021193598A1/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/50—Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03011—L-Glutamate oxidase (1.4.3.11)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/906—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
    • G01N2333/90605—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on the CH-NH2 group of donors (1.4)
    • G01N2333/90633—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3) in general

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2022510522A 2020-03-24 2021-03-23 Ｌ－グルタミン酸オキシダーゼ変異体 Active JP7845176B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2020052809 2020-03-24
JP2020052809 2020-03-24
JP2020139357 2020-08-20
JP2020139357 2020-08-20
PCT/JP2021/011898 WO2021193598A1 (ja) 2020-03-24 2021-03-23 Ｌ－グルタミン酸オキシダーゼ変異体

Publications (2)

Publication Number Publication Date
JPWO2021193598A1 JPWO2021193598A1 (https=) 2021-09-30
JP7845176B2 true JP7845176B2 (ja) 2026-04-14

Family

ID=77890689

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JP2022510522A Active JP7845176B2 (ja) 2020-03-24 2021-03-23 Ｌ－グルタミン酸オキシダーゼ変異体

Country Status (4)

Country Link
US (1) US20230203456A1 (https=)
EP (1) EP4130023A4 (https=)
JP (1) JP7845176B2 (https=)
WO (1) WO2021193598A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2023140286A1 (https=) 2022-01-18 2023-07-27
JP7630148B2 (ja) * 2022-03-16 2025-02-17 東洋濾紙株式会社 アミノ酸定量用試験紙、被検試料中のアミノ酸濃度の定量方法、およびアミノ酸定量用試験紙の製造方法
WO2024090562A1 (ja) 2022-10-27 2024-05-02 キッコーマン株式会社 Ｆａｄ型グルタミン酸デヒドロゲナーゼ

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013146264A (ja) 2011-12-22 2013-08-01 Okayama Univ アミノ酸オキシダーゼ固定化体及びアミノ酸測定装置
CN106282205A (zh) 2015-06-12 2017-01-04 上海市农业科学院 一种高比活l-谷氨酸氧化酶基因多位点突变体及其制备方法和应用
JP2017012169A (ja) 2015-07-06 2017-01-19 ヤマサ醤油株式会社 Ｌ−グルタミン酸測定キット
CN109266664A (zh) 2018-10-23 2019-01-25 南京工业大学 一种利用融合截短表达策略提高谷氨酸氧化酶稳定性的方法
CN110283837A (zh) 2019-04-19 2019-09-27 中国科学院天津工业生物技术研究所 一种高酶活性l-谷氨酸氧化酶突变体及其制备方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5934882A (ja) * 1982-08-23 1984-02-25 Yamasa Shoyu Co Ltd バイオセンサ−
EP1277839B1 (en) 2000-04-19 2008-02-27 Yamasa Corporation L-glutamate oxidase
US9249450B2 (en) * 2013-03-15 2016-02-02 Abbott Point Of Care Inc. Glutamate oxidase mutagenesis for diagnostic testing
CN110283800B (zh) * 2019-08-26 2019-11-05 中国科学院天津工业生物技术研究所 谷氨酸氧化酶突变体、双酶共表达载体及其应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013146264A (ja) 2011-12-22 2013-08-01 Okayama Univ アミノ酸オキシダーゼ固定化体及びアミノ酸測定装置
CN106282205A (zh) 2015-06-12 2017-01-04 上海市农业科学院 一种高比活l-谷氨酸氧化酶基因多位点突变体及其制备方法和应用
JP2017012169A (ja) 2015-07-06 2017-01-19 ヤマサ醤油株式会社 Ｌ−グルタミン酸測定キット
CN109266664A (zh) 2018-10-23 2019-01-25 南京工业大学 一种利用融合截短表达策略提高谷氨酸氧化酶稳定性的方法
CN110283837A (zh) 2019-04-19 2019-09-27 中国科学院天津工业生物技术研究所 一种高酶活性l-谷氨酸氧化酶突变体及其制备方法

Also Published As

Publication number Publication date
US20230203456A1 (en) 2023-06-29
JPWO2021193598A1 (https=) 2021-09-30
EP4130023A1 (en) 2023-02-08
WO2021193598A1 (ja) 2021-09-30
EP4130023A4 (en) 2024-04-24

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