JP7804845B2 - 基板に直接又は間接的に固定化した固定化物質と近接依存性修飾酵素標識された解析対象物質の相互作用の評価方法 - Google Patents

基板に直接又は間接的に固定化した固定化物質と近接依存性修飾酵素標識された解析対象物質の相互作用の評価方法

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JP7804845B2
JP7804845B2 JP2022535409A JP2022535409A JP7804845B2 JP 7804845 B2 JP7804845 B2 JP 7804845B2 JP 2022535409 A JP2022535409 A JP 2022535409A JP 2022535409 A JP2022535409 A JP 2022535409A JP 7804845 B2 JP7804845 B2 JP 7804845B2
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immobilized
protein
substance
seq
amino acid
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JPWO2022009994A5 (https=
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了 森下
修世 杉山
美和子 傳田
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CellFree Sciences Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N37/00—Details not covered by any other group of this subclass

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2022535409A 2020-07-10 2021-07-09 基板に直接又は間接的に固定化した固定化物質と近接依存性修飾酵素標識された解析対象物質の相互作用の評価方法 Active JP7804845B2 (ja)

Applications Claiming Priority (3)

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JP2020119556 2020-07-10
JP2020119556 2020-07-10
PCT/JP2021/026051 WO2022009994A1 (ja) 2020-07-10 2021-07-09 基板に直接又は間接的に固定化した固定化物質と近接依存性修飾酵素標識された解析対象物質の相互作用の評価方法

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JPWO2022009994A1 JPWO2022009994A1 (https=) 2022-01-13
JPWO2022009994A5 JPWO2022009994A5 (https=) 2024-07-09
JP7804845B2 true JP7804845B2 (ja) 2026-01-23

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US (1) US20230251254A1 (https=)
JP (1) JP7804845B2 (https=)
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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025121411A1 (ja) * 2023-12-06 2025-06-12 株式会社セルフリーサイエンス 改変近接依存性修飾酵素

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016512324A (ja) 2013-03-12 2016-04-25 ヴェンタナ メディカル システムズ， インク． 標的物をｉｎｓｉｔｕ検出するための近接アッセイ

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016512324A (ja) 2013-03-12 2016-04-25 ヴェンタナ メディカル システムズ， インク． 標的物をｉｎｓｉｔｕ検出するための近接アッセイ

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BRANON, Tess C，Efficient proximity labeling in living cells and organisms with TurboID，Nature Biotechnology，2018年08月20日，Vol. 36，No. 9，p.880-887，DOI: 10.1038/nbt.4201
FERNANDEZ-SUAREZ, Marta，Protein－Protein Interaction Detection in Vitro and in Cells by Proximity Biotinylation，Journal of the American Chemical Society，2008年06月27日，Vol. 130，No. 29，p.9251-9253，DOI: 10.1021/ja801445p
KIDO Kohki，AirID, a novel proximity biotinylation enzyme, for analysis of proteinprotein interactions，eLife，2020年05月11日，Vol.9, e54983，DOI: 10.7554/eLife.54983
KULYYASSOV Arman，PUB-MS: A Mass Spectrometry-based Method to Monitor ProteinProtein Proximity in vivo，Journal of Proteome Research，2011年09月02日，Vol. 10，No. 10，p.4416-4427，DOI: 10.1021/pr200189p
OOSTDYK, Luke T.，Towards improving proximity labeling by the biotin ligase BirA，Methods，2019年03月，Vol. 157，p.66-79，DOI: 10.1016/j.ymeth.2018.11.003
RAY, Sandipan，Label‐free detection techniques for protein microarrays: Prospects, merits and challenges，PROTEOMICS，2010年02月，Vol. 10，No. 4，p.731-748，DOI: 10.1002/pmic.200900458
REMNANT, Lucy ，In vitro BioID: mapping the CENP-A microenvironment with high temporal and spatial resolution，Molecular Biology of the Cell，2019年05月15日，Vol. 30，No. 11，p.1314-1325，DOI: 10.1091/mbc.E18-12-0799
YUK, Jong Seol，Proteomic applications of surface plasmon resonance biosensors: analysis of protein arrays，Experimental Molecular Medicine，2005年02月01日，Vol. 37，No. 1，p.1-10，DOI: 10.1038/emm.2005.1
ZHOU, Shu-Min，Functional protein microarray: an ideal platform for investigating protein binding property，Frontiers in Biology，2012年06月22日，Vol. 7，No. 4，p.336-349，DOI: 10.1007/s11515-012-1236-9

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WO2022009994A1 (ja) 2022-01-13
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