JP7802707B2 - 核酸を解析するための方法および組成物 - Google Patents

核酸を解析するための方法および組成物

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Publication number
JP7802707B2
JP7802707B2 JP2022579952A JP2022579952A JP7802707B2 JP 7802707 B2 JP7802707 B2 JP 7802707B2 JP 2022579952 A JP2022579952 A JP 2022579952A JP 2022579952 A JP2022579952 A JP 2022579952A JP 7802707 B2 JP7802707 B2 JP 7802707B2
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Japan
Prior art keywords
oligonucleotide
scaffold
nucleic acid
region
ssna
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JP2022579952A
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English (en)
Japanese (ja)
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JP2023531720A (ja
JP2023531720A5 (https=
Inventor
クリストファー ジェイ． トロル，
ヴァーシャ ラオ，
Original Assignee
クラレット バイオサイエンス， エルエルシー
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Publication of JP2023531720A publication Critical patent/JP2023531720A/ja
Priority to JP2025151404A priority Critical patent/JP2025183345A/ja
Publication of JP2023531720A5 publication Critical patent/JP2023531720A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2022579952A 2020-06-24 2021-06-23 核酸を解析するための方法および組成物 Active JP7802707B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025151404A JP2025183345A (ja) 2020-06-24 2025-09-11 核酸を解析するための方法および組成物

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US202063043688P 2020-06-24 2020-06-24
US63/043,688 2020-06-24
US202063086208P 2020-10-01 2020-10-01
US63/086,208 2020-10-01
US202163159174P 2021-03-10 2021-03-10
US63/159,174 2021-03-10
US202163195352P 2021-06-01 2021-06-01
US63/195,352 2021-06-01
PCT/US2021/038609 WO2021262805A1 (en) 2020-06-24 2021-06-23 Methods and compositions for analyzing nucleic acid

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025151404A Division JP2025183345A (ja) 2020-06-24 2025-09-11 核酸を解析するための方法および組成物

Publications (3)

Publication Number Publication Date
JP2023531720A JP2023531720A (ja) 2023-07-25
JP2023531720A5 JP2023531720A5 (https=) 2025-09-24
JP7802707B2 true JP7802707B2 (ja) 2026-01-20

Family

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Family Applications (2)

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JP2022579952A Active JP7802707B2 (ja) 2020-06-24 2021-06-23 核酸を解析するための方法および組成物
JP2025151404A Pending JP2025183345A (ja) 2020-06-24 2025-09-11 核酸を解析するための方法および組成物

Family Applications After (1)

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JP2025151404A Pending JP2025183345A (ja) 2020-06-24 2025-09-11 核酸を解析するための方法および組成物

Country Status (8)

Country Link
US (1) US20230235320A1 (https=)
EP (2) EP4428244B1 (https=)
JP (2) JP7802707B2 (https=)
CN (1) CN116096914A (https=)
AU (1) AU2021297880A1 (https=)
CA (1) CA3186974A1 (https=)
DK (2) DK4172357T3 (https=)
WO (1) WO2021262805A1 (https=)

Families Citing this family (6)

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US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
WO2024097719A1 (en) * 2022-11-02 2024-05-10 Becton, Dickinson And Company Application for peptide nucleic acid (pna) blocker
US20260009070A1 (en) * 2022-12-16 2026-01-08 Foundation Medicine, Inc. Library preparation and analytical methods for preserving topological information of cell-free dna
EP4731784A2 (en) * 2023-06-23 2026-04-29 Seqonce Biosciences, Inc. Nick initiated cycling emission and replacement reaction
WO2025106807A1 (en) * 2023-11-16 2025-05-22 The Board Of Trustees Of The Leland Stanford Junior University Classification of samples using methylation enrichment

Citations (1)

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Publication number Priority date Publication date Assignee Title
US20180087108A1 (en) 2016-06-01 2018-03-29 Roche Sequencing Solutions, Inc. Immuno-pete

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US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
JP5149013B2 (ja) 2004-11-29 2013-02-20 セクエノム，インコーポレイティド メチル化ｄｎａを検出する手段、及び方法
EP3617321B1 (en) 2006-05-31 2024-10-23 Sequenom, Inc. Kit for the extraction and amplification of nucleic acid from a sample
JP2009540802A (ja) 2006-06-16 2009-11-26 セクエノム， インコーポレイテッド サンプルからの核酸を増幅、検出および定量するための方法および組成物
WO2009032779A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for the size-specific seperation of nucleic acid from a sample
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
WO2009120808A2 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
EP3103871B1 (en) 2008-09-16 2020-07-29 Sequenom, Inc. Processes for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for fetal nucleic acid quantification
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP3514244B1 (en) 2009-04-03 2021-07-07 Sequenom, Inc. Nucleic acid preparation methods
WO2011143659A2 (en) 2010-05-14 2011-11-17 Fluidigm Corporation Nucleic acid isolation methods
WO2013019361A1 (en) 2011-07-07 2013-02-07 Life Technologies Corporation Sequencing methods
EP3950944A1 (en) * 2014-09-15 2022-02-09 AbVitro LLC High-throughput nucleotide library sequencing
AU2018212756B2 (en) * 2017-01-27 2021-02-25 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
CN108060191B (zh) * 2017-11-07 2021-05-04 深圳华大智造科技股份有限公司 一种双链核酸片段加接头的方法、文库构建方法和试剂盒
AU2019207900B2 (en) * 2018-01-12 2025-07-10 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
WO2020206143A1 (en) * 2019-04-05 2020-10-08 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
AU2020345621A1 (en) * 2019-09-09 2022-04-07 University Of Utah Research Foundation Targeted sequencing to detect and quantify low levels of methylated DNA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180087108A1 (en) 2016-06-01 2018-03-29 Roche Sequencing Solutions, Inc. Immuno-pete

Also Published As

Publication number Publication date
US20230235320A1 (en) 2023-07-27
EP4428244B1 (en) 2025-10-15
EP4172357B1 (en) 2024-05-15
EP4428244A3 (en) 2024-10-02
AU2021297880A1 (en) 2023-01-19
CN116096914A (zh) 2023-05-09
DK4172357T3 (da) 2024-08-05
JP2023531720A (ja) 2023-07-25
DK4428244T3 (da) 2026-01-12
CA3186974A1 (en) 2021-12-30
JP2025183345A (ja) 2025-12-16
WO2021262805A1 (en) 2021-12-30
EP4172357A1 (en) 2023-05-03
EP4428244A2 (en) 2024-09-11

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