CN116096914A - 用于分析核酸的方法和组合物 - Google Patents

用于分析核酸的方法和组合物 Download PDF

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Publication number
CN116096914A
CN116096914A CN202180058203.4A CN202180058203A CN116096914A CN 116096914 A CN116096914 A CN 116096914A CN 202180058203 A CN202180058203 A CN 202180058203A CN 116096914 A CN116096914 A CN 116096914A
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CN
China
Prior art keywords
nucleic acid
oligonucleotide
scaffold
region
ssna
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Pending
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CN202180058203.4A
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English (en)
Chinese (zh)
Inventor
克里斯多佛·J·特罗尔
V·拉奥
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Clarette Bioscience Co ltd
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Clarette Bioscience Co ltd
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Publication of CN116096914A publication Critical patent/CN116096914A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202180058203.4A 2020-06-24 2021-06-23 用于分析核酸的方法和组合物 Pending CN116096914A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US202063043688P 2020-06-24 2020-06-24
US63/043,688 2020-06-24
US202063086208P 2020-10-01 2020-10-01
US63/086,208 2020-10-01
US202163159174P 2021-03-10 2021-03-10
US63/159,174 2021-03-10
US202163195352P 2021-06-01 2021-06-01
US63/195,352 2021-06-01
PCT/US2021/038609 WO2021262805A1 (en) 2020-06-24 2021-06-23 Methods and compositions for analyzing nucleic acid

Publications (1)

Publication Number Publication Date
CN116096914A true CN116096914A (zh) 2023-05-09

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ID=76943150

Family Applications (1)

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CN202180058203.4A Pending CN116096914A (zh) 2020-06-24 2021-06-23 用于分析核酸的方法和组合物

Country Status (8)

Country Link
US (1) US20230235320A1 (https=)
EP (2) EP4428244B1 (https=)
JP (2) JP7802707B2 (https=)
CN (1) CN116096914A (https=)
AU (1) AU2021297880A1 (https=)
CA (1) CA3186974A1 (https=)
DK (2) DK4172357T3 (https=)
WO (1) WO2021262805A1 (https=)

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US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
WO2024097719A1 (en) * 2022-11-02 2024-05-10 Becton, Dickinson And Company Application for peptide nucleic acid (pna) blocker
US20260009070A1 (en) * 2022-12-16 2026-01-08 Foundation Medicine, Inc. Library preparation and analytical methods for preserving topological information of cell-free dna
EP4731784A2 (en) * 2023-06-23 2026-04-29 Seqonce Biosciences, Inc. Nick initiated cycling emission and replacement reaction
WO2025106807A1 (en) * 2023-11-16 2025-05-22 The Board Of Trustees Of The Leland Stanford Junior University Classification of samples using methylation enrichment

Citations (5)

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CN107002076A (zh) * 2014-09-15 2017-08-01 阿布维特罗有限责任公司 高通量核苷酸文库测序
US20180087108A1 (en) * 2016-06-01 2018-03-29 Roche Sequencing Solutions, Inc. Immuno-pete
CN108060191A (zh) * 2017-11-07 2018-05-22 深圳华大基因科技有限公司 一种双链核酸片段加接头的方法、文库构建方法和试剂盒
WO2019140201A1 (en) * 2018-01-12 2019-07-18 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
CN110248675A (zh) * 2017-01-27 2019-09-17 合成Dna技术公司 利用竞争性链置换构建下一代测序(ngs)文库

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US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
JP5149013B2 (ja) 2004-11-29 2013-02-20 セクエノム，インコーポレイティド メチル化ｄｎａを検出する手段、及び方法
EP3617321B1 (en) 2006-05-31 2024-10-23 Sequenom, Inc. Kit for the extraction and amplification of nucleic acid from a sample
JP2009540802A (ja) 2006-06-16 2009-11-26 セクエノム， インコーポレイテッド サンプルからの核酸を増幅、検出および定量するための方法および組成物
WO2009032779A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for the size-specific seperation of nucleic acid from a sample
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
WO2009120808A2 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
EP3103871B1 (en) 2008-09-16 2020-07-29 Sequenom, Inc. Processes for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for fetal nucleic acid quantification
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP3514244B1 (en) 2009-04-03 2021-07-07 Sequenom, Inc. Nucleic acid preparation methods
WO2011143659A2 (en) 2010-05-14 2011-11-17 Fluidigm Corporation Nucleic acid isolation methods
WO2013019361A1 (en) 2011-07-07 2013-02-07 Life Technologies Corporation Sequencing methods
WO2020206143A1 (en) * 2019-04-05 2020-10-08 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
AU2020345621A1 (en) * 2019-09-09 2022-04-07 University Of Utah Research Foundation Targeted sequencing to detect and quantify low levels of methylated DNA

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107002076A (zh) * 2014-09-15 2017-08-01 阿布维特罗有限责任公司 高通量核苷酸文库测序
US20180087108A1 (en) * 2016-06-01 2018-03-29 Roche Sequencing Solutions, Inc. Immuno-pete
CN110248675A (zh) * 2017-01-27 2019-09-17 合成Dna技术公司 利用竞争性链置换构建下一代测序(ngs)文库
CN108060191A (zh) * 2017-11-07 2018-05-22 深圳华大基因科技有限公司 一种双链核酸片段加接头的方法、文库构建方法和试剂盒
WO2019140201A1 (en) * 2018-01-12 2019-07-18 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid

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Publication number Publication date
US20230235320A1 (en) 2023-07-27
EP4428244B1 (en) 2025-10-15
EP4172357B1 (en) 2024-05-15
EP4428244A3 (en) 2024-10-02
AU2021297880A1 (en) 2023-01-19
DK4172357T3 (da) 2024-08-05
JP2023531720A (ja) 2023-07-25
DK4428244T3 (da) 2026-01-12
CA3186974A1 (en) 2021-12-30
JP2025183345A (ja) 2025-12-16
WO2021262805A1 (en) 2021-12-30
EP4172357A1 (en) 2023-05-03
JP7802707B2 (ja) 2026-01-20
EP4428244A2 (en) 2024-09-11

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