JP7792699B2 - 改変チャネルロドプシン - Google Patents

改変チャネルロドプシン

Info

Publication number
JP7792699B2
JP7792699B2 JP2022510604A JP2022510604A JP7792699B2 JP 7792699 B2 JP7792699 B2 JP 7792699B2 JP 2022510604 A JP2022510604 A JP 2022510604A JP 2022510604 A JP2022510604 A JP 2022510604A JP 7792699 B2 JP7792699 B2 JP 7792699B2
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JP
Japan
Prior art keywords
channelrhodopsin
seq
amino acid
acid sequence
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022510604A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2021193731A1 (https=
JPWO2021193731A5 (https=
Inventor
浩史 冨田
江里子 菅野
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Iwate University
Original Assignee
Iwate University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iwate University filed Critical Iwate University
Publication of JPWO2021193731A1 publication Critical patent/JPWO2021193731A1/ja
Publication of JPWO2021193731A5 publication Critical patent/JPWO2021193731A5/ja
Priority to JP2025134009A priority Critical patent/JP7846932B2/ja
Application granted granted Critical
Publication of JP7792699B2 publication Critical patent/JP7792699B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Ophthalmology & Optometry (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP2022510604A 2020-03-24 2021-03-24 改変チャネルロドプシン Active JP7792699B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025134009A JP7846932B2 (ja) 2020-03-24 2025-08-12 改変チャネルロドプシン

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020053473 2020-03-24
JP2020053473 2020-03-24
PCT/JP2021/012282 WO2021193731A1 (ja) 2020-03-24 2021-03-24 改変チャネルロドプシン

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025134009A Division JP7846932B2 (ja) 2020-03-24 2025-08-12 改変チャネルロドプシン

Publications (3)

Publication Number Publication Date
JPWO2021193731A1 JPWO2021193731A1 (https=) 2021-09-30
JPWO2021193731A5 JPWO2021193731A5 (https=) 2024-08-29
JP7792699B2 true JP7792699B2 (ja) 2025-12-26

Family

ID=77890429

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2022510604A Active JP7792699B2 (ja) 2020-03-24 2021-03-24 改変チャネルロドプシン
JP2025134009A Active JP7846932B2 (ja) 2020-03-24 2025-08-12 改変チャネルロドプシン

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025134009A Active JP7846932B2 (ja) 2020-03-24 2025-08-12 改変チャネルロドプシン

Country Status (5)

Country Link
US (1) US12428453B2 (https=)
EP (1) EP4130271A4 (https=)
JP (2) JP7792699B2 (https=)
CN (1) CN115335525B (https=)
WO (1) WO2021193731A1 (https=)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011019081A1 (ja) 2009-08-10 2011-02-17 国立大学法人東北大学 発現効率が改善された光受容チャネルロドプシン
JP2014500716A (ja) 2010-11-05 2014-01-16 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 光活性化キメラオプシンおよびその使用方法
WO2020059675A1 (ja) 2018-09-20 2020-03-26 国立大学法人岩手大学 改変チャネルロドプシン

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5285521B2 (ja) * 2009-07-08 2013-09-11 日本板硝子株式会社 車両用ガラスアンテナおよび窓ガラス
US8759492B2 (en) 2011-08-17 2014-06-24 The Regents Of The University Of California Engineered red-shifted channelrhodopsin variants
CN109476720A (zh) * 2016-06-03 2019-03-15 马克斯-普朗克科学促进协会 通道视紫红质的突变型光诱导离子通道

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011019081A1 (ja) 2009-08-10 2011-02-17 国立大学法人東北大学 発現効率が改善された光受容チャネルロドプシン
JP2014500716A (ja) 2010-11-05 2014-01-16 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 光活性化キメラオプシンおよびその使用方法
WO2020059675A1 (ja) 2018-09-20 2020-03-26 国立大学法人岩手大学 改変チャネルロドプシン

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YIZHAR, O et al.，Neocortical excitation/inhibition balance in information processing and social dysfunction，Nature，2011年，Vol. 477，pp. 171-178, Supplementary Figure 9

Also Published As

Publication number Publication date
EP4130271A1 (en) 2023-02-08
WO2021193731A1 (ja) 2021-09-30
JP2025159109A (ja) 2025-10-17
US20230331790A1 (en) 2023-10-19
EP4130271A4 (en) 2024-03-06
JP7846932B2 (ja) 2026-04-16
JPWO2021193731A1 (https=) 2021-09-30
US12428453B2 (en) 2025-09-30
CN115335525B (zh) 2025-11-21
CN115335525A (zh) 2022-11-11

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