JP7764247B2 - 細菌送達ビヒクルにおける使用のためのキメラ受容体結合タンパク質 - Google Patents

細菌送達ビヒクルにおける使用のためのキメラ受容体結合タンパク質

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JP7764247B2
JP7764247B2 JP2021529822A JP2021529822A JP7764247B2 JP 7764247 B2 JP7764247 B2 JP 7764247B2 JP 2021529822 A JP2021529822 A JP 2021529822A JP 2021529822 A JP2021529822 A JP 2021529822A JP 7764247 B2 JP7764247 B2 JP 7764247B2
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virus
syn
bacterial
rbp
escherichia
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ヘスス・フェルナンデス－ロドリゲス
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エリゴ・バイオサイエンス
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10111—Myoviridae
    • C12N2795/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10111—Myoviridae
    • C12N2795/10151—Methods of production or purification of viral material
    • C12N2795/10152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2795/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Epidemiology (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2021529822A 2018-11-27 2019-11-26 細菌送達ビヒクルにおける使用のためのキメラ受容体結合タンパク質 Active JP7764247B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201862771761P 2018-11-27 2018-11-27
US62/771,761 2018-11-27
US201962802777P 2019-02-08 2019-02-08
US62/802,777 2019-02-08
PCT/EP2019/082640 WO2020109339A1 (en) 2018-11-27 2019-11-26 Chimeric receptor binding proteins for use in bacterial delivery vehicles

Publications (4)

Publication Number Publication Date
JP2022513141A JP2022513141A (ja) 2022-02-07
JP2022513141A5 JP2022513141A5 (https=) 2022-10-19
JPWO2020109339A5 JPWO2020109339A5 (https=) 2022-10-19
JP7764247B2 true JP7764247B2 (ja) 2025-11-05

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JP2021529822A Active JP7764247B2 (ja) 2018-11-27 2019-11-26 細菌送達ビヒクルにおける使用のためのキメラ受容体結合タンパク質

Country Status (8)

Country Link
US (4) US11661443B2 (https=)
EP (2) EP4374923A3 (https=)
JP (1) JP7764247B2 (https=)
KR (1) KR20210100119A (https=)
CN (1) CN113423824A (https=)
CA (1) CA3120615A1 (https=)
IL (1) IL283089B2 (https=)
WO (1) WO2020109339A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4374923A3 (en) 2018-11-27 2024-08-14 Eligo Bioscience Chimeric receptor binding proteins for use in bacterial delivery vehicles
CN110872614A (zh) * 2019-12-05 2020-03-10 深圳市第三人民医院 基于Cas12a/gRNA的快速鉴定分枝杆菌的方法
US11584781B2 (en) 2019-12-30 2023-02-21 Eligo Bioscience Chimeric receptor binding proteins resistant to proteolytic degradation
US20210196828A1 (en) * 2019-12-30 2021-07-01 Eligo Bioscience Bacterial delivery vehicles for in vivo delivery of a dna payload
WO2022144381A1 (en) 2020-12-30 2022-07-07 Eligo Bioscience Microbiome modulation of a host by delivery of dna payloads with minimized spread
JP2024520940A (ja) * 2021-05-12 2024-05-27 エリゴ・バイオサイエンス 産生細菌細胞及び産生方法におけるその使用
CN116676277B (zh) * 2023-07-28 2023-11-03 中国海洋大学 一种深渊盐单胞菌溶原性噬菌体及其分离方法和应用
CN116715736B (zh) * 2023-08-07 2023-11-14 山东省农业科学院畜牧兽医研究所 噬菌体尾部纤维蛋白在o2抗原血清型的禽致病性大肠杆菌鉴定中的应用

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US8445639B2 (en) 2006-05-15 2013-05-21 Avidbiotics Corporation Recombinant bacteriophage and methods for their use
US10660943B2 (en) 2013-02-07 2020-05-26 The Rockefeller University Sequence specific antimicrobials
EP3234136B1 (en) * 2014-12-16 2024-08-21 C3J Therapeutics, Inc. Compositions of and methods for in vitro viral genome engineering
EP4374923A3 (en) 2018-11-27 2024-08-14 Eligo Bioscience Chimeric receptor binding proteins for use in bacterial delivery vehicles
US11208437B2 (en) 2018-12-21 2021-12-28 Eligo Bioscience Branched receptor binding multi-subunit protein complexes for use in bacterial delivery vehicles

Non-Patent Citations (5)

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Title
ANDO, h., et al.，"Engineering modular viral scaffolds for targeted bacterial population editing."，CELL SYSTEMS，2015年09月23日，Vol.1，pp.187-196，DOI: 10.1016/j.cels.2015.08.013
MONTAG, T., et al.，"A component of the side tail fiber of Escherichia coli bacteriophage lambda can functionally replace the receptor-recognizing part of a long tail fiber protein of the unrelated bacteriophage T4."，JOURNAL OF BACTERIOLOGY，1989年08月，Vol.171, No.8，pp.4378-4384，DOI: 10.1128/jb.171.8.4378-4384.1989
SIPONEN, M., et al.，"Crystal structure of a chimeric receptor binding protein constructed from two lactococcal phages."，JOURNAL OF BACTERIOLOGY，2009年05月，Vol.191, No.10，pp.3220-3225，DOI: 10.1128/jb.01637-08
TETART, F., et al.，"Genome plasticity in the distal tail fiber locus of the T-even bacteriophase: recombination betweem conserved motifs swaps adhesin specificity."，J. MOL. BIOL.，1998年09月25日，Vol.282, No.3，pp.543-556，DOI: 10.1006/jmbi.1998.2047
WANG, J., et al.，"The C-terminal portion of the tail fiber protein of bacteriophage lambda is responsible for binding to LamB, its receptor at the surface of Escherichia coli K-12."，JOURNAL OF BACTERIOLOGY，2000年01月，Vol.182, No.2，pp.508-512，DOI: 10.1128/jb.182.2.508-512.2000

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Publication number Publication date
EP4374923A3 (en) 2024-08-14
US20220112247A1 (en) 2022-04-14
US20200277337A1 (en) 2020-09-03
IL283089B1 (en) 2025-07-01
EP4374923A2 (en) 2024-05-29
KR20210100119A (ko) 2021-08-13
US11661443B2 (en) 2023-05-30
US11512116B2 (en) 2022-11-29
CA3120615A1 (en) 2020-06-04
US20230279058A1 (en) 2023-09-07
CN113423824A (zh) 2021-09-21
IL283089A (en) 2021-06-30
US11236133B2 (en) 2022-02-01
US20200190147A1 (en) 2020-06-18
IL283089B2 (en) 2025-11-01
EP3887511A1 (en) 2021-10-06
JP2022513141A (ja) 2022-02-07
WO2020109339A1 (en) 2020-06-04

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