JP7709707B2 - 新規なイオン伝導性チャネル融合サブユニット及びその使用方法 - Google Patents

新規なイオン伝導性チャネル融合サブユニット及びその使用方法

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Publication number
JP7709707B2
JP7709707B2 JP2023500118A JP2023500118A JP7709707B2 JP 7709707 B2 JP7709707 B2 JP 7709707B2 JP 2023500118 A JP2023500118 A JP 2023500118A JP 2023500118 A JP2023500118 A JP 2023500118A JP 7709707 B2 JP7709707 B2 JP 7709707B2
Authority
JP
Japan
Prior art keywords
ion
channel
protein
luciferase
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023500118A
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English (en)
Japanese (ja)
Other versions
JP2023518616A (ja
JP2023518616A5 (enExample
Inventor
ペルシュランシエ，ヤン
コンパン，ヴァンサン
ラッセンドレン，フランソワ
オリビエ，マチアス
シャップ，ヤン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite de Bordeaux
Institut Polytechnique de Bordeaux
Original Assignee
Universite de Bordeaux
Institut Polytechnique de Bordeaux
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Application filed by Universite de Bordeaux, Institut Polytechnique de Bordeaux filed Critical Universite de Bordeaux
Publication of JP2023518616A publication Critical patent/JP2023518616A/ja
Publication of JP2023518616A5 publication Critical patent/JP2023518616A5/ja
Application granted granted Critical
Publication of JP7709707B2 publication Critical patent/JP7709707B2/ja
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Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2023500118A 2020-03-18 2021-03-15 新規なイオン伝導性チャネル融合サブユニット及びその使用方法 Active JP7709707B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20163892.1A EP3882632A1 (en) 2020-03-18 2020-03-18 Novel ion conducting channel fusion subunits and methods of use thereof
EP20163892.1 2020-03-18
PCT/EP2021/056529 WO2021185759A1 (en) 2020-03-18 2021-03-15 Novel ion conducting channel fusion subunits and methods of use thereof

Publications (3)

Publication Number Publication Date
JP2023518616A JP2023518616A (ja) 2023-05-02
JP2023518616A5 JP2023518616A5 (enExample) 2025-02-27
JP7709707B2 true JP7709707B2 (ja) 2025-07-17

Family

ID=69845921

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023500118A Active JP7709707B2 (ja) 2020-03-18 2021-03-15 新規なイオン伝導性チャネル融合サブユニット及びその使用方法

Country Status (5)

Country Link
US (1) US20230160909A1 (enExample)
EP (2) EP3882632A1 (enExample)
JP (1) JP7709707B2 (enExample)
CA (1) CA3165639A1 (enExample)
WO (1) WO2021185759A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116590304B (zh) * 2023-04-06 2024-01-26 东北农业大学 一种洋葱AcCNGC2基因及其应用
US20260049996A1 (en) * 2024-08-13 2026-02-19 CCLabs Pty Ltd Modification of voltage-gated channels in neurons with fluorescent donor-acceptor pairs
CN121347832B (zh) * 2025-12-19 2026-02-27 五源本草(山东)健康科技有限公司 一种评估精子功能与受精潜能的荧光标记试剂盒及方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018504917A (ja) 2015-02-16 2018-02-22 ユニベルシテ ドゥ ボルドー 新規な電圧依存性イオンチャネル融合物およびその使用方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196524A (en) 1989-01-06 1993-03-23 Eli Lilly And Company Fusion reporter gene for bacterial luciferase
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
US5346815A (en) 1992-08-28 1994-09-13 The Mount Sinai School Of Medicine Of The City University Of New York Sodium ion binding proteins
JP3466765B2 (ja) 1994-07-27 2003-11-17 キッコーマン株式会社 ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
AU765703B2 (en) 1998-03-27 2003-09-25 Bruce J. Bryan Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
EP1088233B9 (en) * 1998-06-16 2006-05-17 PerkinElmer BioSignal Inc. A bioluminescence resonance energy transfer (bret) system and its use
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
ATE320000T1 (de) 1999-12-22 2006-03-15 Perkinelmer Biosignal Inc Ein biolumineszenz resonanz energie transfer (bret) system mit breitem spektralen resolution zwischen donator- und akzeptor-emission wellenlänge und ihre anwendung
DE60319817T2 (de) * 2003-08-04 2009-04-23 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Genetisch-enkodierte Bioindikatoren von Kalziumionen
US8669103B2 (en) 2010-11-02 2014-03-11 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
WO2019055498A1 (en) 2017-09-12 2019-03-21 Igc Bio, Inc. PROSS OPTIMIZED ENZYMES

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018504917A (ja) 2015-02-16 2018-02-22 ユニベルシテ ドゥ ボルドー 新規な電圧依存性イオンチャネル融合物およびその使用方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NATURE COMMUNICATIONS，2016年，Vol.7, No.13268，pp.1-10

Also Published As

Publication number Publication date
US20230160909A1 (en) 2023-05-25
EP3882632A1 (en) 2021-09-22
EP4121761A1 (en) 2023-01-25
WO2021185759A1 (en) 2021-09-23
JP2023518616A (ja) 2023-05-02
CA3165639A1 (en) 2021-09-23

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