CA3165639A1 - Novel ion conducting channel fusion subunits and methods of use thereof - Google Patents

Novel ion conducting channel fusion subunits and methods of use thereof Download PDF

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Publication number
CA3165639A1
CA3165639A1 CA3165639A CA3165639A CA3165639A1 CA 3165639 A1 CA3165639 A1 CA 3165639A1 CA 3165639 A CA3165639 A CA 3165639A CA 3165639 A CA3165639 A CA 3165639A CA 3165639 A1 CA3165639 A1 CA 3165639A1
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CA
Canada
Prior art keywords
channel
protein
ion
probe
subunit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3165639A
Other languages
English (en)
French (fr)
Inventor
Yann Percherancier
Vincent COMPAN
Francois RASSENDREN
Matthias OLLIVIER
Yann CHAPPE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut National de la Sante et de la Recherche Medicale INSERM
Universite de Montpellier
Universite de Bordeaux
Institut Polytechnique de Bordeaux
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut National de la Sante et de la Recherche Medicale INSERM
Universite de Montpellier
Universite de Bordeaux
Institut Polytechnique de Bordeaux
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut National de la Sante et de la Recherche Medicale INSERM, Universite de Montpellier, Universite de Bordeaux, Institut Polytechnique de Bordeaux filed Critical Centre National de la Recherche Scientifique CNRS
Publication of CA3165639A1 publication Critical patent/CA3165639A1/en
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA3165639A 2020-03-18 2021-03-15 Novel ion conducting channel fusion subunits and methods of use thereof Pending CA3165639A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20163892.1A EP3882632A1 (en) 2020-03-18 2020-03-18 Novel ion conducting channel fusion subunits and methods of use thereof
EP20163892.1 2020-03-18
PCT/EP2021/056529 WO2021185759A1 (en) 2020-03-18 2021-03-15 Novel ion conducting channel fusion subunits and methods of use thereof

Publications (1)

Publication Number Publication Date
CA3165639A1 true CA3165639A1 (en) 2021-09-23

Family

ID=69845921

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3165639A Pending CA3165639A1 (en) 2020-03-18 2021-03-15 Novel ion conducting channel fusion subunits and methods of use thereof

Country Status (5)

Country Link
US (1) US20230160909A1 (enExample)
EP (2) EP3882632A1 (enExample)
JP (1) JP7709707B2 (enExample)
CA (1) CA3165639A1 (enExample)
WO (1) WO2021185759A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116590304B (zh) * 2023-04-06 2024-01-26 东北农业大学 一种洋葱AcCNGC2基因及其应用
US20260049996A1 (en) * 2024-08-13 2026-02-19 CCLabs Pty Ltd Modification of voltage-gated channels in neurons with fluorescent donor-acceptor pairs
CN121347832B (zh) * 2025-12-19 2026-02-27 五源本草(山东)健康科技有限公司 一种评估精子功能与受精潜能的荧光标记试剂盒及方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196524A (en) 1989-01-06 1993-03-23 Eli Lilly And Company Fusion reporter gene for bacterial luciferase
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
US5346815A (en) 1992-08-28 1994-09-13 The Mount Sinai School Of Medicine Of The City University Of New York Sodium ion binding proteins
JP3466765B2 (ja) 1994-07-27 2003-11-17 キッコーマン株式会社 ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
AU765703B2 (en) 1998-03-27 2003-09-25 Bruce J. Bryan Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
EP1088233B9 (en) * 1998-06-16 2006-05-17 PerkinElmer BioSignal Inc. A bioluminescence resonance energy transfer (bret) system and its use
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
ATE320000T1 (de) 1999-12-22 2006-03-15 Perkinelmer Biosignal Inc Ein biolumineszenz resonanz energie transfer (bret) system mit breitem spektralen resolution zwischen donator- und akzeptor-emission wellenlänge und ihre anwendung
DE60319817T2 (de) * 2003-08-04 2009-04-23 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Genetisch-enkodierte Bioindikatoren von Kalziumionen
US8669103B2 (en) 2010-11-02 2014-03-11 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
EP3056902B1 (en) * 2015-02-16 2020-04-01 Universite De Bordeaux Novel voltage dependent ion channel fusions and method of use thereof
WO2019055498A1 (en) 2017-09-12 2019-03-21 Igc Bio, Inc. PROSS OPTIMIZED ENZYMES

Also Published As

Publication number Publication date
US20230160909A1 (en) 2023-05-25
EP3882632A1 (en) 2021-09-22
EP4121761A1 (en) 2023-01-25
WO2021185759A1 (en) 2021-09-23
JP2023518616A (ja) 2023-05-02
JP7709707B2 (ja) 2025-07-17

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