JP7708436B2 - 核酸を細胞に送達するための組成物および方法 - Google Patents

核酸を細胞に送達するための組成物および方法

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Publication number
JP7708436B2
JP7708436B2 JP2022513157A JP2022513157A JP7708436B2 JP 7708436 B2 JP7708436 B2 JP 7708436B2 JP 2022513157 A JP2022513157 A JP 2022513157A JP 2022513157 A JP2022513157 A JP 2022513157A JP 7708436 B2 JP7708436 B2 JP 7708436B2
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Prior art keywords
cells
nucleic acid
composition
sequence
seq
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JP2022513157A
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Japanese (ja)
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JP2022546699A (ja
JP2022546699A5 (OSRAM
Inventor
エリアス キジャーノ，
ピーター グレイザー，
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Yale University
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Yale University
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Priority claimed from PCT/US2019/048962 external-priority patent/WO2020047353A1/en
Priority claimed from PCT/US2019/048953 external-priority patent/WO2020047344A1/en
Application filed by Yale University filed Critical Yale University
Publication of JP2022546699A publication Critical patent/JP2022546699A/ja
Publication of JP2022546699A5 publication Critical patent/JP2022546699A5/ja
Priority to JP2025069544A priority Critical patent/JP2025111590A/ja
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Publication of JP7708436B2 publication Critical patent/JP7708436B2/ja
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JP2022513157A 2019-08-30 2020-08-31 核酸を細胞に送達するための組成物および方法 Active JP7708436B2 (ja)

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JP2025069544A JP2025111590A (ja) 2019-08-30 2025-04-21 核酸を細胞に送達するための組成物および方法

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Application Number Priority Date Filing Date Title
USPCT/US2019/048953 2019-08-30
PCT/US2019/048962 WO2020047353A1 (en) 2018-08-31 2019-08-30 Compositions and methods for enhancing triplex and nuclease-based gene editing
PCT/US2019/048953 WO2020047344A1 (en) 2018-08-31 2019-08-30 Compositions and methods for enhancing donor oligonucleotide-based gene editing
USPCT/US2019/048962 2019-08-30
US201962944281P 2019-12-05 2019-12-05
US62/944,281 2019-12-05
PCT/US2020/048823 WO2021042060A1 (en) 2019-08-30 2020-08-31 Compositions and methods for delivery of nucleic acids to cells

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Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3844287B1 (en) 2018-08-31 2024-05-22 Yale University Compositions and methods for enhancing donor oligonucleotide-based gene editing
US12485180B2 (en) * 2019-08-30 2025-12-02 Yale University Compositions and methods for delivery of nucleic acids to cells
IL301014A (en) * 2020-08-31 2023-05-01 Univ Yale Compositions and methods for delivery of nucleic acids to cells
CN117295753A (zh) * 2020-12-04 2023-12-26 基那奥生物公司 用于将核酸递送到细胞的组合物和方法
AU2021390561A1 (en) * 2020-12-04 2023-06-22 Yale University Compositions and methods for delivery of nucleic acids to cells
IL305515A (en) * 2021-03-03 2023-10-01 Univ Yale Preparations and methods for the treatment of skeletal muscle disease
US20240226316A1 (en) * 2021-05-25 2024-07-11 Transmab Pty Ltd Improved immunoglobulin I
IL309662A (en) * 2021-07-02 2024-02-01 Univ Yale Compositions and methods for treating cancers
CN117979994A (zh) * 2021-07-08 2024-05-03 天普大学-联邦高等教育系统 用于增加靶基因和蛋白质的表达的寡核苷酸和病毒未转译区(utr)
EP4395832A1 (en) * 2021-08-31 2024-07-10 Yale University Compositions and methods for treating cancers
US20250161484A1 (en) * 2022-03-03 2025-05-22 Yale University Compositions and methods for treating disease
US20250170259A1 (en) * 2022-03-03 2025-05-29 Yale University Compositions and methods for delivering therapeutic polynucleotides for exon skipping
CN120787163A (zh) * 2022-12-27 2025-10-14 耶鲁大学 抗体药物缀合物
WO2024140936A1 (en) * 2022-12-30 2024-07-04 Cstone Pharmaceuticals (Suzhou) Co., Ltd. Humanized cell-penetrating antibodies
EP4655007A1 (en) * 2023-01-23 2025-12-03 Yale University Antibody oligonucleotide conjugates
WO2025049905A1 (en) * 2023-09-01 2025-03-06 Gennao Bio, Inc. Dnase co-expression in host cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019518040A (ja) 2016-06-15 2019-06-27 イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔｙ 腫瘍に対して標的化されるナノキャリアの抗体媒介性自触反応的送達

Family Cites Families (106)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US150A (en) 1837-03-25 Island
US5436A (en) 1848-02-08 Air-heating furnace
CA197574A (en) 1920-02-24 The Wirebounds Patents Company Wirebound box machine
US4965204A (en) 1984-02-06 1990-10-23 The Johns Hopkins University Human stem cells and monoclonal antibodies
US4714680B1 (en) 1984-02-06 1995-06-27 Univ Johns Hopkins Human stem cells
US5521063A (en) 1985-03-15 1996-05-28 Antivirals Inc. Polynucleotide reagent containing chiral subunits and methods of use
WO1986005519A1 (en) 1985-03-15 1986-09-25 James Summerton Polynucleotide assay reagent and method
US5166315A (en) 1989-12-20 1992-11-24 Anti-Gene Development Group Sequence-specific binding polymers for duplex nucleic acids
US5506337A (en) 1985-03-15 1996-04-09 Antivirals Inc. Morpholino-subunit combinatorial library and method
US5217866A (en) 1985-03-15 1993-06-08 Anti-Gene Development Group Polynucleotide assay reagent and method
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5075109A (en) 1986-10-24 1991-12-24 Southern Research Institute Method of potentiating an immune response
US5422251A (en) 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
US4812397A (en) 1987-02-10 1989-03-14 The Regents Of The University Of California MAB-anti-DNA related to nephritis
US5061620A (en) 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5714331A (en) 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US6441130B1 (en) 1991-05-24 2002-08-27 Isis Pharmaceuticals, Inc. Linked peptide nucleic acids
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5962426A (en) 1993-06-25 1999-10-05 Yale University Triple-helix forming oligonucleotides for targeted mutagenesis
AU691194B2 (en) 1993-06-25 1998-05-14 Yale University Chemically modified oligonucleotide for site-directed mutagenesis
US5527675A (en) 1993-08-20 1996-06-18 Millipore Corporation Method for degradation and sequencing of polymers which sequentially eliminate terminal residues
DE4331012A1 (de) 1993-09-13 1995-03-16 Bayer Ag Nukleinsäuren-bindende Oligomere mit N-Verzweigung für Therapie und Diagnostik
US5409813A (en) 1993-09-30 1995-04-25 Systemix, Inc. Method for mammalian cell separation from a mixture of cell populations
US5677136A (en) 1994-11-14 1997-10-14 Systemix, Inc. Methods of obtaining compositions enriched for hematopoietic stem cells, compositions derived therefrom and methods of use thereof
WO1996039195A2 (en) 1995-06-06 1996-12-12 Yale University Chemically modified oligonucleotide for site-directed mutagenesis
US7279463B2 (en) 1995-06-07 2007-10-09 Yale University Triple-helix forming oligonucleotides for targeted mutagenesis
WO1996040271A1 (en) 1995-06-07 1996-12-19 Yale University Treatment of hemoglobinopathies
EP0910408A4 (en) 1996-03-08 2004-01-14 Univ California DELIVERY SYSTEM WITH MAB 3E10, MUTANTS AND / OR FUNCTIONAL FRAGMENTS THEREOF
US5945337A (en) 1996-10-18 1999-08-31 Quality Biological, Inc. Method for culturing CD34+ cells in a serum-free medium
US5786571A (en) 1997-05-09 1998-07-28 Lexmark International, Inc. Wrapped temperature sensing assembly
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
US6326479B1 (en) 1998-01-27 2001-12-04 Boston Probes, Inc. Synthetic polymers and methods, kits or compositions for modulating the solubility of same
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
WO2001000788A2 (en) 1999-06-25 2001-01-04 Northwestern University Compositions, kits, and methods for modulating survival and differentiation of multi-potential hematopoietic progenitor cells
US6919208B2 (en) 2000-05-22 2005-07-19 The Children's Hospital Of Philadelphia Methods and compositions for enhancing the delivery of a nucleic acid to a cell
KR100872437B1 (ko) 2000-12-01 2008-12-05 막스-플랑크-게젤샤프트 츄어 푀르더룽 데어 비쎈샤프텐 에.파우. Ｒｎａ 간섭을 매개하는 작은 ｒｎａ 분자
US7067617B2 (en) 2001-02-21 2006-06-27 The Scripps Research Institute Zinc finger binding domains for nucleotide sequence ANN
US20040197892A1 (en) 2001-04-04 2004-10-07 Michael Moore Composition binding polypeptides
WO2002094983A2 (en) 2001-05-16 2002-11-28 The Children's Hospital Of Philadelphia Dna-antibody complexes to enhance gene transfer
WO2003016496A2 (en) 2001-08-20 2003-02-27 The Scripps Research Institute Zinc finger binding domains for cnn
EP1468086A2 (en) 2001-12-14 2004-10-20 Yale University INTRACELLULAR GENERATION OF SINGLE−STRANDED DNA
DE102004042546A1 (de) 2004-09-02 2006-03-09 Curevac Gmbh Kombinationstherapie zur Immunstimulation
US20070036773A1 (en) * 2005-08-09 2007-02-15 City Of Hope Generation and application of universal T cells for B-ALL
US8658608B2 (en) 2005-11-23 2014-02-25 Yale University Modified triple-helix forming oligonucleotides for targeted mutagenesis
EP1963499A4 (en) 2005-11-28 2009-04-08 Scripps Research Inst ZINCFINGER BINDING DOMAIN FOR TNN
US20070154989A1 (en) 2006-01-03 2007-07-05 The Scripps Research Institute Zinc finger domains specifically binding agc
CN1986792A (zh) 2006-12-14 2007-06-27 方炳良 致病细胞的外来抗原标记方法
WO2008086529A2 (en) 2007-01-11 2008-07-17 Yale University Compositions and methods for targeted inactivation of hiv cell surface receptors
CA2676234A1 (en) 2007-01-22 2008-07-31 The United States Government As Represented By The Department Of Veteran S Affairs Use of antibody conjugates
EP3486653A1 (en) 2007-05-24 2019-05-22 The United States Government as represented by The Department of Veterans Affairs Treatment of skeletal muscle disorder using ent2
WO2010044894A1 (en) * 2008-10-15 2010-04-22 4S3 Bioscience Inc. Methods and compositions for treatment of myotonic dystrophy
US8309356B2 (en) 2009-04-01 2012-11-13 Yale University Pseudocomplementary oligonucleotides for targeted gene therapy
WO2010123983A1 (en) 2009-04-21 2010-10-28 Yale University Compostions and methods for targeted gene therapy
US9155801B2 (en) * 2009-05-28 2015-10-13 The United States Government As Represented By The Department Of Veterans Affairs Amino acid sequences which enhance peptide conjugate solubility
BRPI1011236A2 (pt) 2009-06-15 2019-07-09 4S3 Bioscience Inc métodos e composições para tratamento de miopatia miotubular usando polipeptídeos quiméricos compreendendo polipeptídeos miotubularesih 1 (mtm1)
US20110268810A1 (en) 2009-11-02 2011-11-03 Yale University Polymeric materials loaded with mutagenic and recombinagenic nucleic acids
NO2510096T3 (OSRAM) 2009-12-10 2015-03-21
US20110293585A1 (en) 2010-04-21 2011-12-01 Helix Therapeutics, Inc. Compositions and methods for treatment of lysosomal storage disorders
US20110262406A1 (en) 2010-04-21 2011-10-27 Yale University Compositions and methods for targeted inactivation of hiv cell surface receptors
ES2744840T3 (es) * 2011-04-01 2020-02-26 Univ Yale Anticuerpos anti-ADN que penetran en las células y usos de los mismos para inhibir la reparación del ADN
US9193758B2 (en) 2011-04-08 2015-11-24 Carnegie Mellon University Center For Technology Transfer & Enterprise Conformationally-preorganized, miniPEG-containing γ-peptide nucleic acids
CA2832569A1 (en) 2011-04-08 2012-10-11 Baylor College Of Medicine Reversing the effects of the tumor microenvironment using chimeric cytokine receptors
US20130071414A1 (en) 2011-04-27 2013-03-21 Gianpietro Dotti Engineered cd19-specific t lymphocytes that coexpress il-15 and an inducible caspase-9 based suicide gene for the treatment of b-cell malignancies
US9272043B2 (en) 2011-12-02 2016-03-01 Yale University Enzymatic synthesis of poly(amine-co-esters) and methods of use thereof for gene delivery
WO2013082529A1 (en) 2011-12-02 2013-06-06 Yale University Enzymatic synthesis of poly(amine-co-esters) and methods of use thereof for gene delivery
WO2013138662A1 (en) 2012-03-16 2013-09-19 4S3 Bioscience, Inc. Antisense conjugates for decreasing expression of dmpk
US9283272B2 (en) 2012-03-30 2016-03-15 The United States Of America As Represented By The Department Of Veterans Affairs Targeting intracellular target-binding determinants with intracellular antibodies
US9732146B2 (en) 2012-03-30 2017-08-15 The United States Of America As Represented By The Department Of Veterans Affairs Antibody-mediated transduction of heat shock proteins into living cells
LT3401400T (lt) 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
US20150290244A1 (en) 2012-07-13 2015-10-15 The Trustees Of The University Of Pennsylvania Use of cart19 to deplete normal b cells to induce tolerance
JP2015527889A (ja) 2012-07-25 2015-09-24 ザ ブロード インスティテュート， インコーポレイテッド 誘導可能なｄｎａ結合タンパク質およびゲノム撹乱ツール、ならびにそれらの適用
WO2014130723A1 (en) 2013-02-20 2014-08-28 Valerion Therapeutics, Llc Methods and compositions for treatment of pompe disease
US20150017120A1 (en) 2013-06-13 2015-01-15 Massachusetts Institute Of Technology Synergistic tumor treatment with extended-pk il-2 and adoptive cell therapy
CA2936102A1 (en) 2014-01-13 2015-07-16 Valerion Therapeutics, Llc Internalizing moieties
WO2015134607A1 (en) 2014-03-04 2015-09-11 Yale University Cell penetrating anti-guanosine antibody based therapy for cancers with ras mutations
MX392639B (es) 2014-03-21 2025-03-24 Univ Leland Stanford Junior Vectores virales recombinantes para la integración de transgenes
IL322264A (en) 2014-04-07 2025-09-01 Novartis Ag Cancer treatment using chimeric antigen receptor (CAR) against CD19
WO2015192092A1 (en) 2014-06-13 2015-12-17 Valerion Therapeutics, Llc Methods and compositions for treatment of glycogen storage diseases and glycogen metabolism disorders
EP3186278A4 (en) 2014-08-27 2018-04-04 Valerion Therapeutics, LLC Internalizing moieties for treatment of cancer
JP2017534571A (ja) 2014-08-28 2017-11-24 イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔｙ 抗体３ｅ１０の多価フラグメントおよびその使用方法
WO2016081621A1 (en) 2014-11-18 2016-05-26 Yale University Formulations for targeted release of agents under low ph conditions and methods of use thereof
WO2017015101A1 (en) 2015-07-17 2017-01-26 University Of Washington Methods for maximizing the efficiency of targeted gene correction
US20190119357A1 (en) 2015-07-23 2019-04-25 Modernatx, Inc. Messenger ribonucleic acids for the production of intracellular binding polypeptides and methods of use thereof
WO2017109177A1 (en) 2015-12-24 2017-06-29 Selexis S.A. Improved eukaryotic cells for protein manufacturing and methods of making them
CA3014795A1 (en) 2016-02-16 2017-08-24 Yale University Compositions and methods for treatment of cystic fibrosis
EP3416976A2 (en) 2016-02-16 2018-12-26 Yale University Compositions for enhancing targeted gene editing and methods of use thereof
KR102775461B1 (ko) 2016-04-01 2025-02-28 어비디티 바이오사이언시스 인크. 핵산-폴리펩타이드 조성물 및 이의 용도
US20200113821A1 (en) 2017-04-04 2020-04-16 Yale University Compositions and methods for in utero delivery
EP3655432A4 (en) 2017-07-17 2021-04-14 Nucleus Therapeutics Pty Ltd BINDING PROTEINS 1
CN111511405A (zh) 2017-10-16 2020-08-07 阿迪根有限公司 用于细胞内递送mRNA的肽和纳米颗粒
US20210137960A1 (en) 2018-02-01 2021-05-13 Yale University Compositions and methods for inhibition of nuclear-penetrating antibodies
WO2019152806A1 (en) 2018-02-01 2019-08-08 Yale University Compositions and methods for enhancing nuclear translocation
EP3844287B1 (en) 2018-08-31 2024-05-22 Yale University Compositions and methods for enhancing donor oligonucleotide-based gene editing
WO2020047353A1 (en) 2018-08-31 2020-03-05 Yale University Compositions and methods for enhancing triplex and nuclease-based gene editing
FI20186112A1 (en) 2018-12-19 2020-06-20 Actim Oy System and method for analysing a point-of-care test result
US12485180B2 (en) 2019-08-30 2025-12-02 Yale University Compositions and methods for delivery of nucleic acids to cells
CN111218458B (zh) 2020-02-27 2020-11-20 珠海丽凡达生物技术有限公司 编码SARS-CoV-2病毒抗原的mRNA和疫苗及疫苗的制备方法
IL301014A (en) 2020-08-31 2023-05-01 Univ Yale Compositions and methods for delivery of nucleic acids to cells
AU2021390561A1 (en) 2020-12-04 2023-06-22 Yale University Compositions and methods for delivery of nucleic acids to cells
JP2025507840A (ja) 2022-03-03 2025-03-21 イエール ユニバーシティ ヒト化３ｅ１０抗体、そのバリアント、及び抗原結合断片

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019518040A (ja) 2016-06-15 2019-06-27 イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔｙ 腫瘍に対して標的化されるナノキャリアの抗体媒介性自触反応的送達

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