JP7642533B2 - 高甘味度甘味料の製造方法 - Google Patents

高甘味度甘味料の製造方法 Download PDF

Info

Publication number
JP7642533B2
JP7642533B2 JP2021524249A JP2021524249A JP7642533B2 JP 7642533 B2 JP7642533 B2 JP 7642533B2 JP 2021524249 A JP2021524249 A JP 2021524249A JP 2021524249 A JP2021524249 A JP 2021524249A JP 7642533 B2 JP7642533 B2 JP 7642533B2
Authority
JP
Japan
Prior art keywords
mogroside
seq
compound
host cell
recombinant host
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2021524249A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022512932A (ja
JP2022512932A5 (enExample
Inventor
ピー. パトロン アンドルー
エダノ ノリーガ クリス
ラオ マナム ラマ
コルキット ジャスティン
フェイバー ネイサン
ジーラー ヘルジ
スティージ ジャスティン
ケイ. オルソン ダニエル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Firmenich Inc
Original Assignee
Firmenich Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Firmenich Inc filed Critical Firmenich Inc
Publication of JP2022512932A publication Critical patent/JP2022512932A/ja
Priority to JP2024174360A priority Critical patent/JP2025000973A/ja
Publication of JP2022512932A5 publication Critical patent/JP2022512932A5/ja
Application granted granted Critical
Publication of JP7642533B2 publication Critical patent/JP7642533B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01005—Dextransucrase (2.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01019—Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Seasonings (AREA)
  • Steroid Compounds (AREA)
JP2021524249A 2018-11-07 2019-11-01 高甘味度甘味料の製造方法 Active JP7642533B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024174360A JP2025000973A (ja) 2018-11-07 2024-10-03 高甘味度甘味料の製造方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862757141P 2018-11-07 2018-11-07
US62/757,141 2018-11-07
PCT/US2019/059498 WO2020096907A1 (en) 2018-11-07 2019-11-01 Methods for making high intensity sweeteners

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2024174360A Division JP2025000973A (ja) 2018-11-07 2024-10-03 高甘味度甘味料の製造方法

Publications (3)

Publication Number Publication Date
JP2022512932A JP2022512932A (ja) 2022-02-07
JP2022512932A5 JP2022512932A5 (enExample) 2024-10-24
JP7642533B2 true JP7642533B2 (ja) 2025-03-10

Family

ID=68655734

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021524249A Active JP7642533B2 (ja) 2018-11-07 2019-11-01 高甘味度甘味料の製造方法
JP2024174360A Pending JP2025000973A (ja) 2018-11-07 2024-10-03 高甘味度甘味料の製造方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2024174360A Pending JP2025000973A (ja) 2018-11-07 2024-10-03 高甘味度甘味料の製造方法

Country Status (10)

Country Link
US (1) US12286661B2 (enExample)
EP (1) EP3860364A1 (enExample)
JP (2) JP7642533B2 (enExample)
KR (1) KR20210089717A (enExample)
CN (1) CN113286523A (enExample)
CA (1) CA3118467A1 (enExample)
MX (1) MX2021005219A (enExample)
PH (1) PH12021551012A1 (enExample)
SG (1) SG11202104584UA (enExample)
WO (1) WO2020096907A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111108213B (zh) 2017-05-03 2024-12-13 弗门尼舍公司 制备高强度甜味剂的方法
CN113286523A (zh) * 2018-11-07 2021-08-20 弗门尼舍公司 制备高强度甜味剂的方法
US12338476B2 (en) 2018-11-07 2025-06-24 Firmenich Incorporated Methods for making high intensity sweeteners
CA3118924A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
WO2021231728A1 (en) * 2020-05-13 2021-11-18 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
US20240093258A1 (en) * 2020-11-24 2024-03-21 Manus Bio Inc. Glycoside product biosynthesis and recovery
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN117098460A (zh) * 2021-01-15 2023-11-21 弗门尼舍公司 包含罗汉果苷的甜味剂组合物及其用途
CN115433702A (zh) * 2022-08-23 2022-12-06 江南大学 一种利用糖基转移酶高效生物合成莱鲍迪苷d2的方法
WO2025144785A2 (en) 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds
CN120060187A (zh) * 2025-04-29 2025-05-30 成都圆大生物科技有限公司 一种固定化酶组合物及其在高效制备瑞鲍迪苷m中的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015536157A (ja) 2012-12-04 2015-12-21 エヴォルヴァ エスアー.Evolva Sa. モグロシド化合物の生合成のための方法および材料
JP2017529860A (ja) 2014-10-01 2017-10-12 エヴォルヴァ エスアー.Evolva Sa. モグロシド化合物の生合成のための方法および材料
WO2018200663A1 (en) 2017-04-25 2018-11-01 The Coca-Cola Company Sweetness and taste improvement of steviol glycoside and mogroside sweeteners with dihydrochalcones

Family Cites Families (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999057250A1 (en) 1998-05-01 1999-11-11 The Procter & Gamble Company Laundry detergent and/or fabric care compositions comprising a modified enzyme
US6468576B1 (en) 2000-06-23 2002-10-22 Nestec S.A. Frozen slush liquid concentrate and method of making same
US7939059B2 (en) 2001-12-10 2011-05-10 California Institute Of Technology Method for the generation of antigen-specific lymphocytes
EP1659881B1 (en) 2003-08-06 2019-04-03 Senomyx Inc. Novel flavors, flavor modifiers, tastants, taste enhancers, umami or sweet tastants, and/or enhancers and use thereof
CN101384183B (zh) 2005-02-04 2012-07-04 西诺米克斯公司 包含连接有杂芳基部分的化合物及其作为用于食物组合物的新型鲜味调节剂、促味剂和味觉增强剂的应用
EP1879471B1 (en) 2005-03-22 2014-12-31 Bio-Soluble Solutions JV, LLC Flavoring composition concentrates
TW200715993A (en) 2005-06-15 2007-05-01 Senomyx Inc Bis-aromatic amides and their uses as sweet flavor modifiers, tastants, and taste enhancers
WO2016130609A1 (en) 2015-02-10 2016-08-18 Purecircle Usa Inc. Rebaudioside m biosynthetic production and recovery methods
DK3235811T3 (en) 2006-04-21 2018-11-12 Senomyx Inc PROCEDURE FOR THE PREPARATION OF OXALAMIDS
EP3031924A1 (en) 2006-05-26 2016-06-15 Amyris, Inc. Production of isoprenoids
EP2074214A2 (en) 2006-09-28 2009-07-01 Microbia, Inc. Production of sterols in oleaginous yeast and fungi
US7928111B2 (en) 2007-06-08 2011-04-19 Senomyx, Inc. Compounds including substituted thienopyrimidinone derivatives as ligands for modulating chemosensory receptors
EP2188300B1 (en) 2007-08-17 2013-01-30 Givaudan SA Novel sweetener iso-mogroside v
AU2008305655B2 (en) 2007-09-20 2013-10-31 Amyris, Inc. Production of isoprenoids
US8119821B2 (en) 2008-02-06 2012-02-21 Senomyx, Inc. Sweetener compositions and methods of making them
TWI429404B (zh) 2008-03-03 2014-03-11 Senomyx Inc 異山梨醇衍生物及彼等作為風味改良劑、促味劑及促味增強劑之用途
MX2011001155A (es) 2008-07-31 2011-07-29 Senomyx Inc Composiciones que comprenden mejoradores del dulzor, y métodos para prepararlas.
EP3085699B1 (en) 2008-07-31 2022-03-02 Firmenich Incorporated Processes for making sweet taste enhancers
WO2010019696A2 (en) 2008-08-12 2010-02-18 Allylix, Inc. Method for production of isoprenoids
MX312376B (es) 2009-04-02 2013-08-15 Amyris Inc Metodos de estabilización e hidrogenacion de olefinas de origen microbiano.
JP5695639B2 (ja) 2009-06-01 2015-04-08 アミリス, インコーポレイテッド 遺伝子改変微生物を作製する方法
HUE034245T2 (en) 2009-11-23 2018-02-28 Nucelis Llc Methods and preparations for the preparation of squalene using yeast
EP2544538B1 (en) 2010-03-12 2022-06-15 PureCircle USA Inc. High-purity steviol glycosides
ES2753523T3 (es) 2010-04-02 2020-04-13 Firmenich Incorporated Modificador del sabor dulce
US8586814B2 (en) 2010-05-21 2013-11-19 Amyris, Inc. Squalane and isosqualane compositions and methods for preparing the same
CN105671108A (zh) * 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
CN103153096B (zh) 2010-08-12 2016-08-03 赛诺米克斯公司 提高甜味增强剂的稳定性的方法和包含稳定的甜味增强剂的组合物
CN103201279A (zh) 2010-11-05 2013-07-10 赛诺米克斯公司 作为trpm8的调节剂有用的化合物
US8962698B2 (en) 2011-01-28 2015-02-24 Tate & Lyle Ingredients Americas Llc Rebaudioside-mogroside V blends
CA2826554C (en) 2011-02-02 2015-03-31 Amyris, Inc. Methods of developing terpene synthase variants
PH12014500184A1 (en) 2011-08-12 2014-03-24 Firmenich Incorporated Sweet flavor modifier
ES2628847T3 (es) 2011-11-09 2017-08-04 Amyris, Inc. Producción de isoprenoides derivados de acetil-coenzima a
EP2783009B1 (en) * 2011-11-23 2016-04-13 Evolva SA Methods and materials for enzymatic synthesis of mogroside compounds
CA3152828A1 (en) 2011-12-19 2013-06-27 The Coca-Cola Company Methods for purifying steviol glycosides and uses of the same
AU2013299859B2 (en) 2012-08-06 2017-06-15 Firmenich Incorporated Sweet flavor modifier
CN104583416A (zh) 2012-08-17 2015-04-29 埃沃尔瓦公司 提高的萜类和类萜产生
JO3155B1 (ar) 2013-02-19 2017-09-20 Senomyx Inc معدِّل نكهة حلوة
WO2014130582A2 (en) 2013-02-19 2014-08-28 Senomyx, Inc. Compounds useful as modulators of trpm8
GB201309077D0 (en) 2013-03-15 2013-07-03 Tate & Lyle Ingredients Improved sweetener
BR112015023089A2 (pt) 2013-03-15 2017-11-21 Amyris Inc célula hospedeira, método para produzir um isoprenoide, e método para aumentar a produção de acetil-coa ou um composto derivado de acetil-coa
EP2970370B1 (en) 2013-03-15 2019-12-04 The Coca-Cola Company Novel mogrosides, compositions and purification methods
MX389727B (es) 2013-12-05 2025-03-20 Analyticon Discovery Gmbh Glucosidos de triterpeno novedosos como edulcorantes o potenciadores de edulcorantes.
US20180020709A1 (en) 2014-04-16 2018-01-25 Purecircle Usa Inc. Rebaudioside m biosynthetic production and recovery methods
CN103923152B (zh) 2014-05-08 2015-11-04 青岛润德生物科技有限公司 一种罗汉果甙v的提取方法
IL287789B2 (en) 2014-09-11 2024-05-01 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Mogroside compounds, methods for their preparation, preparations including them and their uses
EP3208342A4 (en) 2014-10-17 2018-07-04 Suntory Holdings Limited Mogrol glycosyltransferase and gene encoding same
CA2963901A1 (en) 2014-11-07 2016-05-12 Senomyx, Inc. Substituted 4-amino-5-(cyclohexyloxy)quinoline-3-carboxylic acids as sweet flavor modifiers
CN104404065A (zh) * 2014-11-21 2015-03-11 中国科学院天津工业生物技术研究所 罗汉果糖基转移酶基因ugt74ac1及其应用
WO2016120228A1 (en) 2015-01-27 2016-08-04 Montero Gida Sanayi Ve Ticaret A.S. A natural sweetening composition of luo han guo and apple
EP3250050A1 (en) 2015-01-27 2017-12-06 Montero Gida Sanayi Ve Ticaret A.S. A natural sweetening composition
CN105039274A (zh) * 2015-07-13 2015-11-11 中国农业科学院蔬菜花卉研究所 参与西瓜葫芦素e合成的基因簇及其应用
US20180250313A1 (en) 2015-09-08 2018-09-06 Viewpoint Therapeutics, Inc. Compounds and formulations for treating ophthalmic diseases
US20170105432A1 (en) 2015-10-16 2017-04-20 Senomyx, Inc. Sweetener and flavor enhancer formulations
JP6949838B2 (ja) 2015-10-29 2021-10-13 フィルメニッヒ インコーポレイテッドFirmenich Incorporated 高甘味度甘味料
CN109152931A (zh) 2016-03-28 2019-01-04 可口可乐公司 具有类黄酮的甜菊醇糖苷或罗汉果苷甜味剂的甜度和味道改善
CN109219355B (zh) 2016-04-06 2023-03-07 可口可乐公司 甜菊醇糖苷或罗汉果苷甜味剂的甜度和味道改善
WO2018016483A1 (ja) 2016-07-19 2018-01-25 サントリーホールディングス株式会社 モグロールまたはモグロール配糖体の生産方法
CN111108213B (zh) 2017-05-03 2024-12-13 弗门尼舍公司 制备高强度甜味剂的方法
US20170354175A1 (en) 2017-05-15 2017-12-14 Senomyx, Inc. Sweetener composition
US11612175B2 (en) 2017-05-31 2023-03-28 The Coca-Cola Company Sweetness and taste improvement of Steviol glycoside and mogroside sweeteners with cyclamate
EP3638804A1 (en) * 2017-06-15 2020-04-22 Evolva SA Production of mogroside compounds in recombinant hosts
US20180086751A1 (en) 2017-12-01 2018-03-29 Senomyx, Inc. Sweetener composition
US12351849B2 (en) 2018-02-27 2025-07-08 Manus Bio Inc. Microbial production of triterpenoids including mogrosides
CN112423600A (zh) 2018-04-16 2021-02-26 阿尔门德拉私人有限公司 味道调节剂组合物、饮料及其调味组合物
WO2020033420A1 (en) 2018-08-07 2020-02-13 Firmenich Incorporated 5-substituted 4-amino-1h-benzo[c][1,2,6]thiadiazine 2,2-dioxides and formulations and uses thereof
CN113286523A (zh) * 2018-11-07 2021-08-20 弗门尼舍公司 制备高强度甜味剂的方法
US12338476B2 (en) 2018-11-07 2025-06-24 Firmenich Incorporated Methods for making high intensity sweeteners
CA3118924A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
US20220017557A1 (en) 2018-11-27 2022-01-20 Purecircle Usa Inc. High-purity steviol glycosides
CN119344437A (zh) 2019-04-01 2025-01-24 可口可乐公司 含有布拉齐因的组合物
US12227539B2 (en) 2019-04-04 2025-02-18 Firmenich Sa Mogroside compounds and uses thereof
US12458046B2 (en) 2020-04-20 2025-11-04 The Coca-Cola Company Beverages comprising siamenoside I with enhanced flavor
JP2024503101A (ja) 2021-01-15 2024-01-24 フィルメニッヒ インコーポレイテッド モグロシドを含む甘味料組成物およびその使用
JP2024503100A (ja) 2021-01-15 2024-01-24 フィルメニッヒ インコーポレイテッド シアメノシドiを含む甘味料組成物およびその使用
CN117098460A (zh) 2021-01-15 2023-11-21 弗门尼舍公司 包含罗汉果苷的甜味剂组合物及其用途
EP4314316A2 (en) 2021-06-29 2024-02-07 Firmenich Incorporated Methods for making high intensity sweeteners

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015536157A (ja) 2012-12-04 2015-12-21 エヴォルヴァ エスアー.Evolva Sa. モグロシド化合物の生合成のための方法および材料
JP2017529860A (ja) 2014-10-01 2017-10-12 エヴォルヴァ エスアー.Evolva Sa. モグロシド化合物の生合成のための方法および材料
WO2018200663A1 (en) 2017-04-25 2018-11-01 The Coca-Cola Company Sweetness and taste improvement of steviol glycoside and mogroside sweeteners with dihydrochalcones

Also Published As

Publication number Publication date
CA3118467A1 (en) 2020-05-14
US12286661B2 (en) 2025-04-29
PH12021551012A1 (en) 2021-10-04
JP2022512932A (ja) 2022-02-07
MX2021005219A (es) 2021-06-18
JP2025000973A (ja) 2025-01-07
WO2020096907A1 (en) 2020-05-14
US20240218402A1 (en) 2024-07-04
KR20210089717A (ko) 2021-07-16
BR112021008944A2 (pt) 2021-08-17
EP3860364A1 (en) 2021-08-11
SG11202104584UA (en) 2021-06-29
CN113286523A (zh) 2021-08-20

Similar Documents

Publication Publication Date Title
JP7589267B2 (ja) 高強度の甘味料の製造方法
US12338476B2 (en) Methods for making high intensity sweeteners
JP2025000973A (ja) 高甘味度甘味料の製造方法
US11680281B2 (en) Biosynthetic production of steviol glycosides and processes therefore
EP4403045A2 (en) Methods for making high intensity sweeteners
WO2014086890A1 (en) Steviol glycoside compositions sensory properties
BR112021008944B1 (pt) Métodos para fazer edulcorantes de alta intensidade
HK40009689A (en) Biosynthetic production of steviol glycosides and processes therefore

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20221027

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230829

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20231024

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240227

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20240604

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20241003

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20241015

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250128

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250226

R150 Certificate of patent or registration of utility model

Ref document number: 7642533

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150