JP7633149B2 - 新規なエステラーゼ及びその使用 - Google Patents
新規なエステラーゼ及びその使用 Download PDFInfo
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- JP7633149B2 JP7633149B2 JP2021504396A JP2021504396A JP7633149B2 JP 7633149 B2 JP7633149 B2 JP 7633149B2 JP 2021504396 A JP2021504396 A JP 2021504396A JP 2021504396 A JP2021504396 A JP 2021504396A JP 7633149 B2 JP7633149 B2 JP 7633149B2
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- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J11/00—Recovery or working-up of waste materials
- C08J11/04—Recovery or working-up of waste materials of polymers
- C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
- C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
- C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP18306019 | 2018-07-27 | ||
| EP18306019.3 | 2018-07-27 | ||
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| CN107709457B (zh) | 2015-06-12 | 2021-05-25 | 卡比奥斯公司 | 可生物降解聚酯组合物及其用途 |
| MX2018014090A (es) | 2016-05-19 | 2019-04-01 | Carbios | Un proceso para degradar productos de plastico. |
| WO2018011284A1 (en) | 2016-07-12 | 2018-01-18 | Carbios | Novel esterases and uses thereof |
| BR112019012258A2 (pt) | 2016-12-16 | 2020-10-20 | Carbios | proteases de degradação de plástico aprimoradas |
| JP7217267B2 (ja) | 2017-08-31 | 2023-02-02 | キャルビオス | 生物学的実体を含む液体組成物及びその使用 |
| KR20200103757A (ko) | 2017-12-21 | 2020-09-02 | 까르비오 | 신규한 프로테아제 및 이의 용도 |
| JP7601756B2 (ja) | 2018-07-27 | 2024-12-17 | キャルビオス | 新規なエステラーゼ及びその使用 |
| PT3830254T (pt) | 2018-07-27 | 2025-01-24 | Carbios | Novas esterases e suas utilizações |
| FR3088070B1 (fr) | 2018-11-06 | 2021-11-26 | Carbios | Procede de degradation enzymatique de polyethylene terephtalate |
| CA3129549A1 (en) | 2019-03-28 | 2020-10-01 | Carbios | Multicomponent thermoplastic product |
| JP7638952B2 (ja) | 2019-07-11 | 2025-03-04 | キャルビオス | 新規エステラーゼ及びその使用 |
| CA3145635A1 (en) | 2019-07-11 | 2021-01-14 | Carbios | Esterases and uses thereof |
| GB202005073D0 (en) | 2020-04-06 | 2020-05-20 | Mellizyme Biotechnology Ltd | Enzymatic degradation of plastics |
| CN116615491A (zh) | 2020-10-27 | 2023-08-18 | 卡比奥斯公司 | 新酯酶及其用途 |
| DK181658B1 (en) * | 2020-11-12 | 2024-09-11 | Petroleo Brasileiro Sa Petrobras | PROCESS FOR THE PRODUCTION OF GLYCOLIC ACID OR γ-HYDROXYBUTYRIC ACID INTEGRATED WITH THE BIOTECHNOLOGICAL DEPOLYMERISATION OF POLYETYLENE TEREPHTHALATE OR POLY(BUTYLENE SUCCINATE). |
| CN117413007A (zh) | 2021-05-20 | 2024-01-16 | 卡比奥斯公司 | 降解包含至少一种聚酯的塑料产品的方法 |
| AU2022277645A1 (en) | 2021-05-20 | 2023-11-30 | Carbios | Process for degrading a plastic product comprising at least one polyester |
| CN113698742B (zh) * | 2021-08-31 | 2022-11-22 | 江南大学 | 一种聚对苯二甲酸乙二醇酯的改性方法及其在酶解过程中的应用 |
| TW202332768A (zh) * | 2021-11-16 | 2023-08-16 | 法商卡爾畢歐斯公司 | 酯酶之新穎用途 |
| WO2023088909A2 (en) * | 2021-11-16 | 2023-05-25 | Carbios | Novel esterases and uses thereof |
| US20250019678A1 (en) * | 2021-11-16 | 2025-01-16 | Carbios | Esterases and uses thereof |
| WO2023242197A1 (en) | 2022-06-13 | 2023-12-21 | Carbios | Process for purifying mono-ethylene glycol |
| EP4321616A1 (en) | 2022-08-09 | 2024-02-14 | Covestro Deutschland AG | Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers |
| CN115197927B (zh) * | 2022-08-09 | 2024-06-21 | 武汉新华扬生物股份有限公司 | 一种降解生物基塑料的复合酶制剂及其应用 |
| CN115287274B (zh) * | 2022-10-08 | 2023-01-06 | 中国农业科学院生物技术研究所 | 角质酶iccg的突变体及其应用 |
| US20240228727A1 (en) | 2023-01-09 | 2024-07-11 | Protein Evolution Inc. | Systems and methods for pretreatment and/or enzymatic degradation of crystallizable polymers, including copolymers |
| US20240352064A1 (en) | 2023-03-21 | 2024-10-24 | Protein Evolution Inc. | Methods and compositions for the separation of particles from a fluid |
| TW202511480A (zh) | 2023-05-15 | 2025-03-16 | 法商卡爾畢歐斯公司 | 新穎之酯酶及其用途 |
| CN121175416A (zh) | 2023-05-15 | 2025-12-19 | 卡比奥斯公司 | 酯酶变体及其在聚酯降解中的用途 |
| CN117264927B (zh) * | 2023-09-19 | 2024-11-29 | 源天生物科技(天津)有限公司 | PET降解酶PET-mh29/124/183突变体及其编码基因、工程菌和应用 |
| CN117448297B (zh) * | 2023-09-19 | 2024-12-03 | 源天生物科技(天津)有限公司 | PET降解酶PET-mh183/208突变体及其编码基因、工程菌和应用 |
| CN117187212B (zh) * | 2023-09-19 | 2025-02-11 | 源天生物科技(天津)有限公司 | PET降解酶PET-mh突变体及其编码基因、重组质粒、工程菌和应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018011284A1 (en) | 2016-07-12 | 2018-01-18 | Carbios | Novel esterases and uses thereof |
| WO2018011281A1 (en) | 2016-07-12 | 2018-01-18 | Carbios | Novel esterases and uses thereof |
Family Cites Families (18)
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| WO2012099018A1 (ja) * | 2011-01-19 | 2012-07-26 | 天野エンザイム株式会社 | 枝葉コンポスト由来新規エステラーゼ |
| FR2984354A1 (fr) | 2011-12-20 | 2013-06-21 | Centre Nat Rech Scient | Procede de preparation d'alliage polymere/enzymes |
| EP2922906B1 (en) | 2012-11-20 | 2018-10-31 | Carbios | Method for recycling plastic products |
| EP3065890A2 (en) | 2013-11-05 | 2016-09-14 | Carbios | A method for degrading a plastic |
| DE102014204374A1 (de) * | 2014-03-11 | 2015-09-17 | Henkel Ag & Co. Kgaa | PET-Esterasen und deren Verwendung |
| CA2947478C (en) | 2014-05-16 | 2022-09-13 | Carbios | Process of recycling mixed pet plastic articles |
| TN2017000085A1 (en) | 2014-10-21 | 2018-07-04 | Carbios | Polypeptide having a polyester degrading activity and uses thereof |
| US10626242B2 (en) | 2014-12-19 | 2020-04-21 | Carbios | Plastic compound and preparation process |
| EP3268469A1 (en) | 2015-03-13 | 2018-01-17 | Carbios | New polypeptide having a polyester degrading activity and uses thereof |
| CN107709457B (zh) | 2015-06-12 | 2021-05-25 | 卡比奥斯公司 | 可生物降解聚酯组合物及其用途 |
| US10717996B2 (en) | 2015-12-21 | 2020-07-21 | Carbios | Recombinant yeast cells producing polylactic acid and uses thereof |
| MX2018014090A (es) | 2016-05-19 | 2019-04-01 | Carbios | Un proceso para degradar productos de plastico. |
| BR112019012258A2 (pt) | 2016-12-16 | 2020-10-20 | Carbios | proteases de degradação de plástico aprimoradas |
| WO2019043134A1 (en) | 2017-08-31 | 2019-03-07 | Carbiolice | BIODEGRADABLE POLYESTER ARTICLE COMPRISING ENZYMES |
| JP7217267B2 (ja) | 2017-08-31 | 2023-02-02 | キャルビオス | 生物学的実体を含む液体組成物及びその使用 |
| KR20200103757A (ko) | 2017-12-21 | 2020-09-02 | 까르비오 | 신규한 프로테아제 및 이의 용도 |
| JP7601756B2 (ja) | 2018-07-27 | 2024-12-17 | キャルビオス | 新規なエステラーゼ及びその使用 |
| PT3830254T (pt) | 2018-07-27 | 2025-01-24 | Carbios | Novas esterases e suas utilizações |
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| WO2018011284A1 (en) | 2016-07-12 | 2018-01-18 | Carbios | Novel esterases and uses thereof |
| WO2018011281A1 (en) | 2016-07-12 | 2018-01-18 | Carbios | Novel esterases and uses thereof |
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| EP3830270A1 (en) | 2021-06-09 |
| US20210171921A1 (en) | 2021-06-10 |
| JP2021531032A (ja) | 2021-11-18 |
| CN112673104B (zh) | 2025-02-25 |
| WO2020021117A1 (en) | 2020-01-30 |
| KR20210042122A (ko) | 2021-04-16 |
| CN112673104A (zh) | 2021-04-16 |
| US11692181B2 (en) | 2023-07-04 |
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