CN112673104B - 新酯酶及其用途 - Google Patents

新酯酶及其用途 Download PDF

Info

Publication number
CN112673104B
CN112673104B CN201980057623.3A CN201980057623A CN112673104B CN 112673104 B CN112673104 B CN 112673104B CN 201980057623 A CN201980057623 A CN 201980057623A CN 112673104 B CN112673104 B CN 112673104B
Authority
CN
China
Prior art keywords
esterase
polyester
seq
amino acid
esterases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201980057623.3A
Other languages
English (en)
Chinese (zh)
Other versions
CN112673104A (zh
Inventor
I·安德烈
V·图尼尔
A·吉勒
S·迪奎斯内
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carbios SA
Original Assignee
Carbios SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carbios SA filed Critical Carbios SA
Publication of CN112673104A publication Critical patent/CN112673104A/zh
Application granted granted Critical
Publication of CN112673104B publication Critical patent/CN112673104B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201980057623.3A 2018-07-27 2019-07-26 新酯酶及其用途 Active CN112673104B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18306019 2018-07-27
EP18306019.3 2018-07-27
PCT/EP2019/070288 WO2020021117A1 (en) 2018-07-27 2019-07-26 Novel esterases and uses thereof

Publications (2)

Publication Number Publication Date
CN112673104A CN112673104A (zh) 2021-04-16
CN112673104B true CN112673104B (zh) 2025-02-25

Family

ID=63144938

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980057623.3A Active CN112673104B (zh) 2018-07-27 2019-07-26 新酯酶及其用途

Country Status (6)

Country Link
US (1) US11692181B2 (https=)
EP (1) EP3830270A1 (https=)
JP (1) JP7633149B2 (https=)
KR (1) KR20210042122A (https=)
CN (1) CN112673104B (https=)
WO (1) WO2020021117A1 (https=)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107709457B (zh) 2015-06-12 2021-05-25 卡比奥斯公司 可生物降解聚酯组合物及其用途
MX2018014090A (es) 2016-05-19 2019-04-01 Carbios Un proceso para degradar productos de plastico.
WO2018011284A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
BR112019012258A2 (pt) 2016-12-16 2020-10-20 Carbios proteases de degradação de plástico aprimoradas
JP7217267B2 (ja) 2017-08-31 2023-02-02 キャルビオス 生物学的実体を含む液体組成物及びその使用
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
JP7601756B2 (ja) 2018-07-27 2024-12-17 キャルビオス 新規なエステラーゼ及びその使用
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
CA3129549A1 (en) 2019-03-28 2020-10-01 Carbios Multicomponent thermoplastic product
JP7638952B2 (ja) 2019-07-11 2025-03-04 キャルビオス 新規エステラーゼ及びその使用
CA3145635A1 (en) 2019-07-11 2021-01-14 Carbios Esterases and uses thereof
GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
CN116615491A (zh) 2020-10-27 2023-08-18 卡比奥斯公司 新酯酶及其用途
DK181658B1 (en) * 2020-11-12 2024-09-11 Petroleo Brasileiro Sa Petrobras PROCESS FOR THE PRODUCTION OF GLYCOLIC ACID OR γ-HYDROXYBUTYRIC ACID INTEGRATED WITH THE BIOTECHNOLOGICAL DEPOLYMERISATION OF POLYETYLENE TEREPHTHALATE OR POLY(BUTYLENE SUCCINATE).
CN117413007A (zh) 2021-05-20 2024-01-16 卡比奥斯公司 降解包含至少一种聚酯的塑料产品的方法
AU2022277645A1 (en) 2021-05-20 2023-11-30 Carbios Process for degrading a plastic product comprising at least one polyester
CN113698742B (zh) * 2021-08-31 2022-11-22 江南大学 一种聚对苯二甲酸乙二醇酯的改性方法及其在酶解过程中的应用
TW202332768A (zh) * 2021-11-16 2023-08-16 法商卡爾畢歐斯公司 酯酶之新穎用途
WO2023088909A2 (en) * 2021-11-16 2023-05-25 Carbios Novel esterases and uses thereof
US20250019678A1 (en) * 2021-11-16 2025-01-16 Carbios Esterases and uses thereof
WO2023242197A1 (en) 2022-06-13 2023-12-21 Carbios Process for purifying mono-ethylene glycol
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
CN115197927B (zh) * 2022-08-09 2024-06-21 武汉新华扬生物股份有限公司 一种降解生物基塑料的复合酶制剂及其应用
CN115287274B (zh) * 2022-10-08 2023-01-06 中国农业科学院生物技术研究所 角质酶iccg的突变体及其应用
US20240228727A1 (en) 2023-01-09 2024-07-11 Protein Evolution Inc. Systems and methods for pretreatment and/or enzymatic degradation of crystallizable polymers, including copolymers
US20240352064A1 (en) 2023-03-21 2024-10-24 Protein Evolution Inc. Methods and compositions for the separation of particles from a fluid
TW202511480A (zh) 2023-05-15 2025-03-16 法商卡爾畢歐斯公司 新穎之酯酶及其用途
CN121175416A (zh) 2023-05-15 2025-12-19 卡比奥斯公司 酯酶变体及其在聚酯降解中的用途
CN117264927B (zh) * 2023-09-19 2024-11-29 源天生物科技(天津)有限公司 PET降解酶PET-mh29/124/183突变体及其编码基因、工程菌和应用
CN117448297B (zh) * 2023-09-19 2024-12-03 源天生物科技(天津)有限公司 PET降解酶PET-mh183/208突变体及其编码基因、工程菌和应用
CN117187212B (zh) * 2023-09-19 2025-02-11 源天生物科技(天津)有限公司 PET降解酶PET-mh突变体及其编码基因、重组质粒、工程菌和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) * 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
WO2015135757A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa Pet-esterasen und deren verwendung

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
EP2922906B1 (en) 2012-11-20 2018-10-31 Carbios Method for recycling plastic products
EP3065890A2 (en) 2013-11-05 2016-09-14 Carbios A method for degrading a plastic
CA2947478C (en) 2014-05-16 2022-09-13 Carbios Process of recycling mixed pet plastic articles
TN2017000085A1 (en) 2014-10-21 2018-07-04 Carbios Polypeptide having a polyester degrading activity and uses thereof
US10626242B2 (en) 2014-12-19 2020-04-21 Carbios Plastic compound and preparation process
EP3268469A1 (en) 2015-03-13 2018-01-17 Carbios New polypeptide having a polyester degrading activity and uses thereof
CN107709457B (zh) 2015-06-12 2021-05-25 卡比奥斯公司 可生物降解聚酯组合物及其用途
US10717996B2 (en) 2015-12-21 2020-07-21 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
MX2018014090A (es) 2016-05-19 2019-04-01 Carbios Un proceso para degradar productos de plastico.
CA3030469C (en) * 2016-07-12 2024-04-30 Carbios Esterases and uses thereof
WO2018011284A1 (en) 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
BR112019012258A2 (pt) 2016-12-16 2020-10-20 Carbios proteases de degradação de plástico aprimoradas
WO2019043134A1 (en) 2017-08-31 2019-03-07 Carbiolice BIODEGRADABLE POLYESTER ARTICLE COMPRISING ENZYMES
JP7217267B2 (ja) 2017-08-31 2023-02-02 キャルビオス 生物学的実体を含む液体組成物及びその使用
KR20200103757A (ko) 2017-12-21 2020-09-02 까르비오 신규한 프로테아제 및 이의 용도
JP7601756B2 (ja) 2018-07-27 2024-12-17 キャルビオス 新規なエステラーゼ及びその使用
PT3830254T (pt) 2018-07-27 2025-01-24 Carbios Novas esterases e suas utilizações

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012099018A1 (ja) * 2011-01-19 2012-07-26 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
WO2015135757A1 (de) * 2014-03-11 2015-09-17 Henkel Ag & Co. Kgaa Pet-esterasen und deren verwendung

Also Published As

Publication number Publication date
EP3830270A1 (en) 2021-06-09
US20210171921A1 (en) 2021-06-10
JP2021531032A (ja) 2021-11-18
JP7633149B2 (ja) 2025-02-19
WO2020021117A1 (en) 2020-01-30
KR20210042122A (ko) 2021-04-16
CN112673104A (zh) 2021-04-16
US11692181B2 (en) 2023-07-04

Similar Documents

Publication Publication Date Title
CN112673104B (zh) 新酯酶及其用途
CN112654701B (zh) 新酯酶及其用途
CN112639101B (zh) 新酯酶及其用途
CN114096665B (zh) 新型酯酶及其用途
CN109642221B (zh) 新酯酶及其用途
CN109563494B (zh) 新酯酶及其用途
CN114096664B (zh) 新型酯酶及其用途
JP2023546505A (ja) 新規エステラーゼ及びその使用
US20230392129A1 (en) Novel esterases and uses thereof
JP2024545360A (ja) エステラーゼ及びその使用
CN121175416A (zh) 酯酶变体及其在聚酯降解中的用途

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant