JP7565621B2 - 蛍光模倣明視野イメージング - Google Patents

蛍光模倣明視野イメージング Download PDF

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JP7565621B2
JP7565621B2 JP2022520687A JP2022520687A JP7565621B2 JP 7565621 B2 JP7565621 B2 JP 7565621B2 JP 2022520687 A JP2022520687 A JP 2022520687A JP 2022520687 A JP2022520687 A JP 2022520687A JP 7565621 B2 JP7565621 B2 JP 7565621B2
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tissue sample
tissue
image
excitation light
light
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Japanese (ja)
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JP2022551846A (ja
JP2022551846A5 (https=
Inventor
フェレイドウニ，ファルザド
レベンソン，リチャード・エム
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University of California San Diego UCSD
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University of California San Diego UCSD
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/12—Condensers affording bright-field illumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06193—Secondary in situ sources, e.g. fluorescent particles

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Microscoopes, Condenser (AREA)
JP2022520687A 2019-10-03 2020-10-02 蛍光模倣明視野イメージング Active JP7565621B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962910197P 2019-10-03 2019-10-03
US62/910,197 2019-10-03
PCT/US2020/053976 WO2021067726A1 (en) 2019-10-03 2020-10-02 Fluorescence imitating brightfield imaging

Publications (3)

Publication Number Publication Date
JP2022551846A JP2022551846A (ja) 2022-12-14
JP2022551846A5 JP2022551846A5 (https=) 2023-05-18
JP7565621B2 true JP7565621B2 (ja) 2024-10-11

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JP2022520687A Active JP7565621B2 (ja) 2019-10-03 2020-10-02 蛍光模倣明視野イメージング

Country Status (4)

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US (1) US11808703B2 (https=)
EP (1) EP4038370A4 (https=)
JP (1) JP7565621B2 (https=)
WO (1) WO2021067726A1 (https=)

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WO2021067726A1 (en) * 2019-10-03 2021-04-08 The Regents Of The University Of California Fluorescence imitating brightfield imaging
CN118020113A (zh) * 2021-10-12 2024-05-10 香港科技大学 用于无标记多重组织化学虚拟染色的系统和方法
US20230162410A1 (en) * 2021-11-22 2023-05-25 Cytoveris Inc. Multi-spectral Auto-fluorescence based Stainless and Slide-free Virtual histology
CN119044140A (zh) * 2024-10-15 2024-11-29 重庆生强医疗科技有限公司 用于荧光原位杂交的单通道三色扫描方法、系统及其应用

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WO2012029752A1 (ja) 2010-08-31 2012-03-08 コニカミノルタエムジー株式会社 生体物質検出方法
JP2016161417A (ja) 2015-03-02 2016-09-05 キヤノン株式会社 画像処理装置、画像処理方法、イメージングシステム
JP2016223931A (ja) 2015-06-01 2016-12-28 コニカミノルタ株式会社 蛍光画像の合焦システム、合焦方法および合焦プログラム
JP2017524913A (ja) 2014-07-03 2017-08-31 トーレス、リチャード 組織処理及び撮像の新規な方法
WO2019147909A1 (en) 2018-01-26 2019-08-01 University Of Rochester Methods for ultraviolet excitation microscopy of biological surfaces

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US7217266B2 (en) * 2001-05-30 2007-05-15 Anderson R Rox Apparatus and method for laser treatment with spectroscopic feedback
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Publication number Priority date Publication date Assignee Title
US20090118622A1 (en) 2007-11-06 2009-05-07 The Regents Of The University Of California APPARATUS AND METHOD FOR WIDEFIELD FUNCTIONAL IMAGING (WiFI) USING INTEGRATED STRUCTURED ILLUMINATION AND LASER SPECKLE IMAGING
WO2012029752A1 (ja) 2010-08-31 2012-03-08 コニカミノルタエムジー株式会社 生体物質検出方法
JP2017524913A (ja) 2014-07-03 2017-08-31 トーレス、リチャード 組織処理及び撮像の新規な方法
JP2016161417A (ja) 2015-03-02 2016-09-05 キヤノン株式会社 画像処理装置、画像処理方法、イメージングシステム
JP2016223931A (ja) 2015-06-01 2016-12-28 コニカミノルタ株式会社 蛍光画像の合焦システム、合焦方法および合焦プログラム
WO2019147909A1 (en) 2018-01-26 2019-08-01 University Of Rochester Methods for ultraviolet excitation microscopy of biological surfaces

Also Published As

Publication number Publication date
CA3156802A1 (en) 2021-04-08
US11808703B2 (en) 2023-11-07
EP4038370A4 (en) 2023-10-11
WO2021067726A1 (en) 2021-04-08
JP2022551846A (ja) 2022-12-14
EP4038370A1 (en) 2022-08-10
US20230131930A1 (en) 2023-04-27

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