JP7548581B2 - 収量が改善されたｄｎａの合成 - Google Patents

収量が改善されたｄｎａの合成 Download PDF

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JP7548581B2
JP7548581B2 JP2021507969A JP2021507969A JP7548581B2 JP 7548581 B2 JP7548581 B2 JP 7548581B2 JP 2021507969 A JP2021507969 A JP 2021507969A JP 2021507969 A JP2021507969 A JP 2021507969A JP 7548581 B2 JP7548581 B2 JP 7548581B2
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dna
cell
salts
nucleotide
synthesis
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JP2021534746A (ja
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ポーター，ニール
ロスウェル，ポール・ジェームズ
ゲーリッグ・ジャンニーニ，ステファニー
バブラ，アンナ
アディー，トーマス・アントニー・ジェームズ
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タッチライト・アイピー・リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
JP2021507969A 2018-08-17 2019-08-16 収量が改善されたｄｎａの合成 Active JP7548581B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024074647A JP2024099814A (ja) 2018-08-17 2024-05-02 収量が改善されたｄｎａの合成

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1813429.6A GB201813429D0 (en) 2018-08-17 2018-08-17 Synthesis of DNA with improved yield
GB1813429.6 2018-08-17
PCT/GB2019/052307 WO2020035698A1 (en) 2018-08-17 2019-08-16 Synthesis of dna with improved yield

Related Child Applications (1)

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JP2024074647A Division JP2024099814A (ja) 2018-08-17 2024-05-02 収量が改善されたｄｎａの合成

Publications (2)

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JP2021534746A JP2021534746A (ja) 2021-12-16
JP7548581B2 true JP7548581B2 (ja) 2024-09-10

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JP2021507969A Active JP7548581B2 (ja) 2018-08-17 2019-08-16 収量が改善されたｄｎａの合成
JP2024074647A Pending JP2024099814A (ja) 2018-08-17 2024-05-02 収量が改善されたｄｎａの合成

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JP2024074647A Pending JP2024099814A (ja) 2018-08-17 2024-05-02 収量が改善されたｄｎａの合成

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US (1) US20210301312A1 (enExample)
EP (1) EP3837382A1 (enExample)
JP (2) JP7548581B2 (enExample)
KR (1) KR20210084431A (enExample)
CN (1) CN113056565A (enExample)
AU (1) AU2019321778B2 (enExample)
BR (1) BR112021002764A2 (enExample)
CA (1) CA3109755A1 (enExample)
GB (1) GB201813429D0 (enExample)
IL (1) IL280817B2 (enExample)
MX (1) MX2021001729A (enExample)
SG (1) SG11202101548YA (enExample)
WO (1) WO2020035698A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202002068D0 (en) * 2020-02-14 2020-04-01 Touchlight Ip Ltd DNA synthesis yield improvements
GB202111742D0 (en) 2021-08-16 2021-09-29 Touchlight Ip Ltd Improved process
US20250027107A1 (en) 2021-10-18 2025-01-23 Flagship Pioneering Innovations Vii, Llc Dna compositions and related methods
GB202319998D0 (en) 2023-12-22 2024-02-07 Touchlight Ip Ltd duplexing method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017525384A (ja) 2014-09-05 2017-09-07 タッチライト・アイピー・リミテッド Ｄｎａの合成

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8435774B2 (en) * 2006-06-28 2013-05-07 Qiagen Gmbh Enhancing reactivation of thermostable reversibly inactivated enzymes
US8986930B2 (en) * 2010-07-12 2015-03-24 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
CN103649333B (zh) * 2011-04-20 2017-02-15 梅撒生物科技公司 用于核酸检测和鉴定的集成装置
EP2798089B1 (en) * 2011-12-30 2018-05-23 Bio-rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
US9587263B2 (en) * 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017525384A (ja) 2014-09-05 2017-09-07 タッチライト・アイピー・リミテッド Ｄｎａの合成

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KLENOW, H. et al.，Effect of monovalent cations on the activity of the DNA polymerase of Escherichia coli B，European Journal of Biochemistry，1969年05月01日，Vol. 9，P. 133-141
MONTGOMERY, J. L. et al.，Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments，Clinical Chemistry，2013年10月15日，Vol. 60:2，P. 334-340
WALWICK, E. R. et al.，Monovalent cation effects on a deoxyribonucleic acid-synthesizing system，BIOCHIM. BIOPHYS. ACTA，1962年12月31日，Vol. 61，P. 876-884

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Publication number Publication date
EP3837382A1 (en) 2021-06-23
AU2019321778B2 (en) 2025-05-08
GB201813429D0 (en) 2018-10-03
WO2020035698A1 (en) 2020-02-20
AU2019321778A1 (en) 2021-02-25
IL280817B2 (en) 2025-08-01
CA3109755A1 (en) 2020-02-20
CN113056565A (zh) 2021-06-29
US20210301312A1 (en) 2021-09-30
IL280817B1 (en) 2025-04-01
MX2021001729A (es) 2021-04-19
JP2024099814A (ja) 2024-07-25
IL280817A (en) 2021-04-29
BR112021002764A2 (pt) 2021-07-20
JP2021534746A (ja) 2021-12-16
KR20210084431A (ko) 2021-07-07
SG11202101548YA (en) 2021-03-30

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