JP7529284B2 - Ｒｎａのキャッピング方法、修飾ｒｎａの製造方法、及び修飾ｒｎａ - Google Patents

Ｒｎａのキャッピング方法、修飾ｒｎａの製造方法、及び修飾ｒｎａ Download PDF

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JP7529284B2
JP7529284B2 JP2021512300A JP2021512300A JP7529284B2 JP 7529284 B2 JP7529284 B2 JP 7529284B2 JP 2021512300 A JP2021512300 A JP 2021512300A JP 2021512300 A JP2021512300 A JP 2021512300A JP 7529284 B2 JP7529284 B2 JP 7529284B2
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rna
carbon atoms
gtp
compound
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JPWO2020204130A1 (https=
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博英 齊藤
博久 大野
冴 赤峰
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Kyoto University NUC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/0705—Nucleotidyltransferases (2.7.7) mRNA guanylyltransferase (2.7.7.50)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
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  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021512300A 2019-04-05 2020-04-02 Ｒｎａのキャッピング方法、修飾ｒｎａの製造方法、及び修飾ｒｎａ Active JP7529284B2 (ja)

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JP2019073163 2019-04-05
JP2019073163 2019-04-05
PCT/JP2020/015168 WO2020204130A1 (ja) 2019-04-05 2020-04-02 Ｒｎａのキャッピング方法、修飾ｒｎａの製造方法、及び修飾ｒｎａ

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JP7529284B2 true JP7529284B2 (ja) 2024-08-06

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US (1) US20220170062A1 (https=)
EP (1) EP3950699A4 (https=)
JP (1) JP7529284B2 (https=)
WO (1) WO2020204130A1 (https=)

Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
EP4509608A4 (en) * 2022-03-04 2025-10-08 Japan Science & Tech Agency CAPPED RNA AND PRODUCTION METHOD THEREOF, PROTEIN PRODUCTION APPARATUS, AND PROTEIN PRODUCTION METHOD
WO2026014365A1 (ja) * 2024-07-08 2026-01-15 国立大学法人東海国立大学機構 ５´キャップアナログ

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015535430A (ja) 2012-11-26 2015-12-14 モデルナ セラピューティクス インコーポレイテッドＭｏ 末端修飾ｒｎａ
JP2017500034A (ja) 2013-12-13 2017-01-05 モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 修飾核酸分子及びその使用
US20170175129A1 (en) 2014-06-19 2017-06-22 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
JP2017523777A (ja) 2014-07-17 2017-08-24 モデルナティエックス インコーポレイテッドＭｏｄｅｒｎａＴＸ，Ｉｎｃ． ポリヌクレオチドの末端修飾

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2010659B1 (en) * 2006-04-14 2014-06-18 CellScript, Inc. Kits and methods for generating 5' capped RNA
GB0706243D0 (en) * 2007-03-30 2007-05-09 Univ Southampton Modified nucleic acids
US11479766B2 (en) * 2013-12-05 2022-10-25 New England Biolabs, Inc. Methods for labeling a population of RNA molecules
JP2019073163A (ja) 2017-10-17 2019-05-16 三菱自動車工業株式会社 四輪操舵車両

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015535430A (ja) 2012-11-26 2015-12-14 モデルナ セラピューティクス インコーポレイテッドＭｏ 末端修飾ｒｎａ
JP2017500034A (ja) 2013-12-13 2017-01-05 モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 修飾核酸分子及びその使用
US20170175129A1 (en) 2014-06-19 2017-06-22 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
JP2017523777A (ja) 2014-07-17 2017-08-24 モデルナティエックス インコーポレイテッドＭｏｄｅｒｎａＴＸ，Ｉｎｃ． ポリヌクレオチドの末端修飾

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WARMINSKI, M., et al.，Applications of phosphate modification and labeling to study (m)RNA caps.，Topics in Current Chemistry，2017年，Vol.375:16，pp.1-29

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EP3950699A4 (en) 2023-01-18
JPWO2020204130A1 (https=) 2020-10-08
EP3950699A1 (en) 2022-02-09
US20220170062A1 (en) 2022-06-02
WO2020204130A1 (ja) 2020-10-08

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