JP7505691B2 - 胚盤胞形成促進剤 - Google Patents
胚盤胞形成促進剤 Download PDFInfo
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- JP7505691B2 JP7505691B2 JP2020072222A JP2020072222A JP7505691B2 JP 7505691 B2 JP7505691 B2 JP 7505691B2 JP 2020072222 A JP2020072222 A JP 2020072222A JP 2020072222 A JP2020072222 A JP 2020072222A JP 7505691 B2 JP7505691 B2 JP 7505691B2
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- Prior art keywords
- blastocyst
- medium
- yeast
- extract
- garlic
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Classifications
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Description
特に、マウスの体外受精においては、2細胞まで分裂した後、細胞分裂が進行しなくなる現象、2cell blockが知られており、受精率は向上しても、胚盤胞への形成率が低ければ、妊娠率は向上しない(非特許文献1)。
[1]酵母発酵ニンニク又はその抽出物を含有する胚盤胞形成促進剤。
[2]受精卵から胚盤胞への形成促進剤である[1]記載の胚盤胞形成促進剤。
[3]酵母発酵ニンニク又はその抽出物を含有する体外受精用培地。
[4]酵母発酵ニンニク又はその抽出物を含有する培地中で卵子と精子を受精させ、次いで当該培地中で受精卵を培養することを特徴とする体外受精用胞胚又は胚盤胞の製造法。
従って、酵母発酵ニンニク又はその抽出物は、胚盤胞形成促進剤、特に受精卵から胚盤胞への形成促進剤として有用である。また、酵母発酵ニンニク又はその抽出物は、体外受精用培地の成分としても有用であり、酵母発酵ニンニク又はその抽出物を含有する培地で、受精卵を培養すれば、体外受精用胞胚又は胚盤胞の形成が促進される。
製造は、常法に従い、各種の製剤化に適した手段により行うことができる。また、本発明の胚盤胞形成促進剤の形態としては、錠剤、ソフトカプセル、ハードカプセル、顆粒、ドリンク剤、膣用外用剤、ジェリー状外用剤等が好ましい。
生ニンニクの剥き身100gを、ミキサーを用いてペースト化し、次いで75~85℃に30分加熱した。次いで、酵母(saccharomyces cerevisiae)を白金耳で植菌しグルコースを添加し、35℃で約3日間発酵させた。85℃に30分加熱して発酵停止した後、凍結乾燥機で48時間凍結乾燥し、酵母発酵ニンニク末を得た(収量30g)。
(試験方法)
供試動物
本実験では、2匹の雄C57BL/6J及びBALB/cマウス(共に12週齢)から採精した。また、6匹の雌C57BL/6Jマウス(4週齢)から採卵した。
精子の前培養には、CARD FERTIUPTM精子前培養培地(九動、佐賀、日本)を使用した。卵子塊の前培養にはCARD MEDIUMTM高性能体外受精用培地(九動、佐賀、日本)を使用した。受精卵の培養にはCARD mHTF体外受精用培地(九動、佐賀、日本)を使用した。2細胞胚の培養にはKSOM-AA(MERCK、NJ、USA)を使用した。
供試動物を頸椎脱臼により安楽死させた後、両側の精巣上体尾部を摘出し、付着した血液及び脂肪を取り除いた。ピンセットで精巣上体尾部を固定して、ノエス剪刀で尾部中央の精巣上体を切開した。精巣上体尾部切開部から精子塊を解剖針ですくい取り、FERTIUPTM精子前培養培地(九動、佐賀、日本)で30分間前培養(37℃、5%CO2、95% air)した後に、酵母発酵にんにく抽出液(1%)を含むFERTIUPTM精子前培養培地(九動、佐賀、日本)で60分間前培養(37℃、5%CO2、95% air)した。コントロールは、同様に30分間前培養(37℃、5%CO2、95% air)した後に、にんにく抽出液を含まないFERTIUPTM精子前培養培地(九動、佐賀、日本)で60分間前培養(37℃、5%CO2、95% air)した。
雌C57BL/6Jマウス(4週齢)に0.1mlのCARD HyperOvaTM過剰排卵誘起剤(九動、佐賀、日本)を腹腔内注射し、その48時間後に37.5IU/mlのヒト絨毛性性腺刺激ホルモンhCG(ゴナドトロピン、あすか製薬株式会社、東京、日本)を腹腔内注射して過剰排卵を誘発した。hCG投与の16時間後に過剰排卵させた雌マウスを頸椎脱臼により安楽死させて卵管を摘出し、細胞培養用流動パラフィン(ナカライテクス、京都、日本)内で卵管膨大部を開裂し、卵子塊を採取しCARD MEDIUMTM(九動、佐賀、日本)で前培養した。
前培養した精子と卵子塊を体外受精した(37℃、5%CO2、95% air)。3時間後、受精卵をCARD mHTF体外受精用培地(九動、佐賀、日本)で3回洗浄し、一晩培養した(37℃、5%CO2、95% air)。翌日、2細胞期胚をKSOM-AA(MERCK、NJ、USA)に移して3日間培養した(37℃、5%CO2、95% air)。
体外受精を実施した翌日に、2細胞期胚及び未受精卵、死亡卵をそれぞれカウントして受精率(%、2細胞期胚数/すべての細胞数)を算出した。また、2細胞期胚をKSOM-AA(MERCK、NJ、USA)に移して3日間培養し、胚盤胞及び2細胞期胚、死亡胚をそれぞれカウントして胚盤胞率(胚盤胞数/すべての胚数)及び2細胞期胚率(2細胞期胚数/すべての胚数)、死亡胚率(死亡胚数/すべての胚数)を算出した。
すべてのデータは平均±標準誤差(SEM)で表記した。統計解析はStudent t検定にて実施し、p<0.05を統計学的に有意であると判定した。
2細胞胚までの収率及び胚盤胞形成までの収率を図1に示す。
図1より、酵母発酵ニンニクの抽出物を添加して培養された受精卵は、2細胞胚までの割合はコントロールと比較して同等であった。一方、酵母発酵ニンニクの抽出物を添加して培養された受精卵は、胚盤胞までの形成率がコントロールに比べて有意に増加した。
従って、酵母発酵ニンニク又はその抽出物は、受精卵から胚盤胞への形成を促進させる作用を有することがわかった。
Claims (4)
- 酵母発酵ニンニク又はその抽出物を含有する胚盤胞形成促進剤。
- 受精卵から胚盤胞への形成促進剤である請求項1記載の胚盤胞形成促進剤。
- 酵母発酵ニンニク又はその抽出物(生ニンニク水性抽出液を酵母で発酵させて得られた抽出成分を除く)を含有する体外受精用培地。
- 酵母発酵ニンニク又はその抽出物を含有する培地中で卵子と精子を受精させ、次いで当該培地中で受精卵を培養することを特徴とする、ヒトを除く哺乳動物の体外受精用胞胚又は胚盤胞の製造法。
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JP2001299261A (ja) | 2000-04-26 | 2001-10-30 | Seibutsu Nogyo Kenkyusho:Kk | ニンニクの無臭化方法及び健康食品 |
JP2017514894A (ja) | 2014-04-22 | 2017-06-08 | ヒョンス・チュン | ニンニク醗酵組成物の製造方法及びその方法で製造されたニンニク醗酵組成物 |
JP2020132611A (ja) | 2019-02-26 | 2020-08-31 | 株式会社天真堂 | オートファジー調節剤 |
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JP2001299261A (ja) | 2000-04-26 | 2001-10-30 | Seibutsu Nogyo Kenkyusho:Kk | ニンニクの無臭化方法及び健康食品 |
JP2017514894A (ja) | 2014-04-22 | 2017-06-08 | ヒョンス・チュン | ニンニク醗酵組成物の製造方法及びその方法で製造されたニンニク醗酵組成物 |
JP2020132611A (ja) | 2019-02-26 | 2020-08-31 | 株式会社天真堂 | オートファジー調節剤 |
Non-Patent Citations (1)
Title |
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吉崎嘉一ら,酵母発酵にんにくのマウスの受精と初期胚発生に及ぼす影響の検討, 薬理と治療, 2020.7.20,第48巻, 第7号,pp.1145-1150 |
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