JP7502029B2 - 修飾されたアルデヒド：フェレドキシンオキシドレダクターゼ活性を有する微生物および関連方法 - Google Patents

修飾されたアルデヒド：フェレドキシンオキシドレダクターゼ活性を有する微生物および関連方法 Download PDF

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JP7502029B2
JP7502029B2 JP2019512182A JP2019512182A JP7502029B2 JP 7502029 B2 JP7502029 B2 JP 7502029B2 JP 2019512182 A JP2019512182 A JP 2019512182A JP 2019512182 A JP2019512182 A JP 2019512182A JP 7502029 B2 JP7502029 B2 JP 7502029B2
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clostridium
strain
coa
naturally occurring
ethanol
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JP2019514441A (ja
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リュウ，ファンミン
ケプケ，マイケル
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ランザテク，インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/07—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
    • C12Y102/07005—Aldehyde ferredoxin oxidoreductase (1.2.7.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
JP2019512182A 2016-05-14 2017-05-12 修飾されたアルデヒド：フェレドキシンオキシドレダクターゼ活性を有する微生物および関連方法 Active JP7502029B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022115092A JP2022163046A (ja) 2016-05-14 2022-07-19 修飾されたアルデヒド：フェレドキシンオキシドレダクターゼ活性を有する微生物および関連方法

Applications Claiming Priority (3)

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US201662336639P 2016-05-14 2016-05-14
US62/336,639 2016-05-14
PCT/US2017/032564 WO2017200884A1 (en) 2016-05-14 2017-05-12 Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods

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JP2019514441A JP2019514441A (ja) 2019-06-06
JP2019514441A5 JP2019514441A5 (enExample) 2020-06-25
JP7502029B2 true JP7502029B2 (ja) 2024-06-18

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JP2022115092A Pending JP2022163046A (ja) 2016-05-14 2022-07-19 修飾されたアルデヒド：フェレドキシンオキシドレダクターゼ活性を有する微生物および関連方法

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US (1) US10294498B2 (enExample)
EP (1) EP3464559A4 (enExample)
JP (2) JP7502029B2 (enExample)
KR (2) KR102514023B1 (enExample)
CN (1) CN109477063A (enExample)
CA (1) CA3024114C (enExample)
EA (1) EA201892627A1 (enExample)
SG (1) SG11201810031RA (enExample)
WO (1) WO2017200884A1 (enExample)
ZA (1) ZA201807617B (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017200884A1 (en) * 2016-05-14 2017-11-23 Lanzatech, Inc. Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods
US11976314B2 (en) * 2017-08-29 2024-05-07 Gwangju Institute Of Science And Technology Method for converting non-ethanol producing, acetogenic strain to ethanol-producing strain and method for producing ethanol from same ethanol-producing strain by using carbon monoxide
CN109929862B (zh) * 2019-03-14 2022-09-16 云南农业大学 一种从反刍动物瘤胃宏转录组数据筛选纤维素酶基因进行克隆的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016065217A1 (en) 2014-10-22 2016-04-28 Lanzatech New Zealand Limited Multi-stage bioreactor processes

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
NZ546496A (en) 2006-04-07 2008-09-26 Lanzatech New Zealand Ltd Gas treatment process
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
NZ553984A (en) 2007-03-19 2009-07-31 Lanzatech New Zealand Ltd Alcohol production process
US8222013B2 (en) 2007-11-13 2012-07-17 Lanzatech New Zealand Limited Bacteria and methods of use thereof
CA2727549C (en) 2008-06-09 2014-08-26 Lanzatech New Zealand Limited Production of butanediol by anaerobic microbial fermentation
US8039239B2 (en) 2008-12-16 2011-10-18 Coskata, Inc. Recombinant microorganisms having modified production of alcohols and acids
CN102791869B (zh) 2010-03-10 2015-08-19 朗泽科技新西兰有限公司 通过发酵的酸产生
WO2011114238A2 (en) * 2010-03-18 2011-09-22 Universität Regensburg Shuttle vector based transformation system for pyrococcus furiosus
EP2606138A2 (en) 2010-08-19 2013-06-26 Lanzatech New Zealand Limited A process for producing chemicals using microbial fermentation of substrates comprising carbon monoxide
EP2609206A4 (en) 2010-08-26 2014-07-09 Lanzatech New Zealand Ltd PROCESS FOR THE PRODUCTION OF ETHANOL AND ETHYLENE BY FERMENTATION
US20110236941A1 (en) 2010-10-22 2011-09-29 Lanzatech New Zealand Limited Recombinant microorganism and methods of production thereof
AU2012221176B2 (en) 2011-02-25 2015-03-19 Lanzatech Nz, Inc. Recombinant microorganisms and uses therefor
WO2012131627A1 (en) * 2011-03-31 2012-10-04 Lanzatech New Zealand Limited A fermentation process for controlling butanediol production
ES2788510T3 (es) 2011-09-08 2020-10-21 Lanzatech New Zealand Ltd Proceso de fermentación
CA2874832C (en) 2012-05-30 2016-02-02 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
WO2013185123A1 (en) 2012-06-08 2013-12-12 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel
KR102121888B1 (ko) 2012-08-28 2020-06-12 란자테크 뉴질랜드 리미티드 재조합 미생물 및 이에 대한 용도
MY183766A (en) * 2013-03-15 2021-03-12 Lanzatech New Zealand Ltd A system and method for controlling metabolite production in a microbial fermentation
US20150079650A1 (en) * 2013-06-05 2015-03-19 Lanzatech New Zealand Limited Recombinant microorganisms exhibiting increased flux through a fermentation pathway
US20150275238A1 (en) * 2014-03-28 2015-10-01 University Of Georgia Research Foundation, Inc. Genetically engineered microbes and methods for converting organic acids to alcohols
CN107636147B (zh) 2015-05-27 2021-09-10 朗泽科技新西兰有限公司 用于制造分支酸衍生物的基因工程化微生物
KR20180056785A (ko) 2015-10-13 2018-05-29 란자테크 뉴질랜드 리미티드 에너지-생성 발효 경로를 포함하는 유전자 조작된 박테리아
WO2017200884A1 (en) * 2016-05-14 2017-11-23 Lanzatech, Inc. Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016065217A1 (en) 2014-10-22 2016-04-28 Lanzatech New Zealand Limited Multi-stage bioreactor processes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Ｂａｓｅｎ，Ｍ．ｅｔ ａｌ．，ＰＮＡＳ，２０１４，Ｖｏｌ．１１１，Ｎｏ．４９， ｐ．１７６１８－１７６２３
Ｆｌｉｔｓｃｈ，Ｓ．Ｋ．，Ｕｎｉｖｅｒｓｉｔｙ ｏｆ Ｕｌｍ，２０１５，［検索日２０２１．０４．０８］，インターネット ＜ＵＲＬ：ｈｔｔｐｓ：／／ｏｐａｒｕ．ｕｎｉ－ｕｌｍ．ｄｅ／ｘｍｌｕｉ／ｂｉｔｓｔｒｅａｍ／ｈａｎｄｌｅ／１２３４５６７８９／３３５２／ｖｔｓ＿９５７６＿１４４９６．ｐｄｆ？ｓｅｑｕｅｎｃｅ＝１＆ｉｓＡｌｌｏｗｅｄ＝ｙ＞，ｐ．Ｉ－ＩＶ，１－１５２

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Publication number Publication date
EP3464559A1 (en) 2019-04-10
JP2019514441A (ja) 2019-06-06
CA3024114A1 (en) 2017-11-23
CN109477063A (zh) 2019-03-15
CA3024114C (en) 2020-02-04
ZA201807617B (en) 2021-04-28
US10294498B2 (en) 2019-05-21
US20170327849A1 (en) 2017-11-16
SG11201810031RA (en) 2018-12-28
EA201892627A1 (ru) 2019-04-30
KR20230044031A (ko) 2023-03-31
KR102514023B1 (ko) 2023-03-23
EP3464559A4 (en) 2020-02-12
JP2022163046A (ja) 2022-10-25
KR20190017790A (ko) 2019-02-20
WO2017200884A1 (en) 2017-11-23

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