CA3024114C - Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods - Google Patents

Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods Download PDF

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Publication number
CA3024114C
CA3024114C CA3024114A CA3024114A CA3024114C CA 3024114 C CA3024114 C CA 3024114C CA 3024114 A CA3024114 A CA 3024114A CA 3024114 A CA3024114 A CA 3024114A CA 3024114 C CA3024114 C CA 3024114C
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Prior art keywords
clostridium
strain
naturally occurring
bacterium
autoethanogenum
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CA3024114A
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English (en)
French (fr)
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CA3024114A1 (en
Inventor
Fungmin Liew
Michael Koepke
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Lanzatech Inc
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Lanzatech Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/065Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16Butanols
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001Alcohol dehydrogenase (1.1.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/07Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
    • C12Y102/07005Aldehyde ferredoxin oxidoreductase (1.2.7.5)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
CA3024114A 2016-05-14 2017-05-12 Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods Active CA3024114C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662336639P 2016-05-14 2016-05-14
US62/336,639 2016-05-14
PCT/US2017/032564 WO2017200884A1 (en) 2016-05-14 2017-05-12 Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods

Publications (2)

Publication Number Publication Date
CA3024114A1 CA3024114A1 (en) 2017-11-23
CA3024114C true CA3024114C (en) 2020-02-04

Family

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Family Applications (1)

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CA3024114A Active CA3024114C (en) 2016-05-14 2017-05-12 Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods

Country Status (10)

Country Link
US (1) US10294498B2 (enExample)
EP (1) EP3464559A4 (enExample)
JP (2) JP7502029B2 (enExample)
KR (2) KR20230044031A (enExample)
CN (1) CN109477063A (enExample)
CA (1) CA3024114C (enExample)
EA (1) EA201892627A1 (enExample)
SG (1) SG11201810031RA (enExample)
WO (1) WO2017200884A1 (enExample)
ZA (1) ZA201807617B (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017200884A1 (en) * 2016-05-14 2017-11-23 Lanzatech, Inc. Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods
KR102073308B1 (ko) * 2017-08-29 2020-02-05 광주과학기술원 에탄올 비생산성 아세토젠 균주를 에탄올 생성균주로 전환하는 방법 및 상기 에탄올 생성균주로부터 일산화탄소를 이용한 에탄올의 제조방법
CN109929862B (zh) * 2019-03-14 2022-09-16 云南农业大学 一种从反刍动物瘤胃宏转录组数据筛选纤维素酶基因进行克隆的方法

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UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
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NZ589632A (en) 2008-06-09 2013-03-28 Lanzatech New Zealand Ltd Production of butanediol by anaerobic microbial fermentation
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EP2606138A2 (en) 2010-08-19 2013-06-26 Lanzatech New Zealand Limited A process for producing chemicals using microbial fermentation of substrates comprising carbon monoxide
EP2609206A4 (en) 2010-08-26 2014-07-09 Lanzatech New Zealand Ltd PROCESS FOR THE PRODUCTION OF ETHANOL AND ETHYLENE BY FERMENTATION
US20110236941A1 (en) 2010-10-22 2011-09-29 Lanzatech New Zealand Limited Recombinant microorganism and methods of production thereof
WO2012115527A2 (en) 2011-02-25 2012-08-30 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
CN103582703A (zh) * 2011-03-31 2014-02-12 新西兰郎泽科技公司 一种用于控制丁二醇生产的发酵方法
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Also Published As

Publication number Publication date
ZA201807617B (en) 2021-04-28
JP7502029B2 (ja) 2024-06-18
JP2022163046A (ja) 2022-10-25
KR20190017790A (ko) 2019-02-20
WO2017200884A1 (en) 2017-11-23
US20170327849A1 (en) 2017-11-16
KR102514023B1 (ko) 2023-03-23
EA201892627A1 (ru) 2019-04-30
JP2019514441A (ja) 2019-06-06
EP3464559A1 (en) 2019-04-10
CN109477063A (zh) 2019-03-15
CA3024114A1 (en) 2017-11-23
EP3464559A4 (en) 2020-02-12
US10294498B2 (en) 2019-05-21
SG11201810031RA (en) 2018-12-28
KR20230044031A (ko) 2023-03-31

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