JP7423026B1 - バイオフィルムを破壊するための溶液及びその製造方法 - Google Patents
バイオフィルムを破壊するための溶液及びその製造方法 Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
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Abstract
Description
特許文献2に記載の発明は、嚢胞性線維症患者におけるバイオフィルムの破壊方法に関するもので、単離されたかまたは組換え型の組込み宿主因子(IHF)ポリペプチド、またはそのそれぞれの断片もしくは等価物を用いている。
この発明の目的は、人体にやさしく、バイオフィルムを効果的に破壊する溶液とその製造方法を提供することである。
本発明の殺菌用溶液は、水(H2O)を用いて重水素硫酸(D2SO4)のpHを調製して作られたものである。重水素硫酸(D2SO4)は、Cambridge Isotope Laboratories, Inc.製のものを用いた。水(H2O)は、水道水を用いた。本発明のバイオフィルムを破壊するための溶液は、これら2つの物質から構成されるものであるが、pH値に影響を与えない、若しくは影響が少ない微量成分を添加してもよい。pHの測定は、堀場製作所製のモデルMETRO-51を用いた。
実際の細胞外多糖類(EPS)の代わりの多糖類として、水あめと植物性ゼリーを用いて、多糖類が本発明によってどのように分解されるのかを調べるための予備試験を行った。水あめは、加藤産業株式会社製のもの(商品名「Kanpy 水あめ」)を用い、植物性ゼリーは、COOP製(製造者:伊那食品工業株式会社)のもの(商品名「コープアガー」)を用いた。
撮影に用いた顕微鏡は、Life technologies社製のEVOS XL coreを用いた。吸光度測定器は、CORONA ELECTRIC社SH-1000 Labを用いた。データ処理ソフトウェアは「SF6」とし、吸光度波長は、590nmとした。
ストレプトコッカス・ミュータンス菌を2%スクロース含有THBで希釈し、細胞培養用プレート(Thermo scientificshaNunclon Delta Surface 96well)に100μl播種したものを10個用意した。播種と同時に、重水素硫酸(D2SO4)を水(H2O)で希釈し、pH=0.8、1.2、1.5、2.3、2.7、3.0、3.3、3.7、4.0に調製した試験液100mlを添加した。なお、播種した10個のうち1個については、試験液は添加しなかった。
ストレプトコッカス・ミュータンス菌を2%スクロース含有THBで希釈し、細胞培養用プレート(Thermo scientific社Nunclon Delta Surface 96well)に100μl播種したものを10個用意した。その後、マルチガスインキュベーターで24時間培養してプレート底面にバイオフィルムを形成させた。
ストレプトコッカス・ミュータンス菌を2%スクロース含有THBで希釈し、細胞培養用プレート(Thermo scientific社Nunclon Delta Surface 96well)に100μl播種したものを10個用意した。その後、マルチガスインキュベーターで24時間培養してプレート底面にバイオフィルムを形成させた。
ミュータンス菌は、糖を栄養源として酸を産生することから、菌の密集した局所でpH低下に耐えられるように耐酸性の性質がこのシステムで活性化される。また、菌の増殖に歯止めがかけられるように殺菌物質であるバクテリオンを産生し、菌量の調節を行う。
ミュータンス菌は、環境に適応するために遺伝子の取り込みが活性化され、厳しい環境でも生息できる性質を持つように変異しやすくする。
このように、ミュータンス菌では、低pH環境におかれても、このようなシステムを有しているため死滅させることが困難となり、pHが0.5以上2.0以下という低いpHが必要となる。
この菌は、ミュータンス菌のようなクオラムセンシングシステムを有さないので、抗酸性はミュータンス菌よりも低く、pHがミュータンス菌と比べて高いpHを有するバイオフィルム破壊液でもバイオフィルムを破壊することができる。
Fn菌を2%スクロース含有THBで希釈し、細胞培養用プレート(Thermo scientificshaNunclon Delta Surface 96well)に100μl播種したものを10個用意した。播種と同時に、重水素硫酸(D2SO4)を水(H2O)で希釈し、pH=0.5、2.0、3.0、4.0、5.0、6.0に調製した試験液100mlを添加した。なお、播種した7個のうち1個については、試験液は添加しなかった。
Claims (3)
- 水(H2O)と重水素硫酸(D2SO4)からなり、pHが0.5以上4.0以下の、
バイオフィルムを破壊するための溶液。 - 水(H2O)を用いて重水素硫酸(D2SO4)を希釈して、水(H2O)と重水素硫
酸(D2SO4)からなり、pHが0.5以上4.0以下の、バイオフィルムを破壊する
ための溶液を製造する方法。 - 請求項1のバイオフィルムを破壊するための溶液を用いて、バイオフィルムを破壊する
方法。
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Citations (6)
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WO2008065734A1 (fr) | 2006-11-27 | 2008-06-05 | Oct Incorporated | Composition aqueuse |
JP2014100700A (ja) | 2012-10-23 | 2014-06-05 | Okuto:Kk | 汚染土壌の除染方法 |
JP2016013520A (ja) | 2014-07-02 | 2016-01-28 | 株式会社オクト | 飲料水 |
JP2017500409A5 (ja) | 2014-12-17 | 2018-01-25 | ||
JP2021165284A (ja) | 2020-01-07 | 2021-10-14 | ロート製薬株式会社 | アクネ菌バイオフィルム破壊組成物 |
JP6966818B1 (ja) | 2021-04-30 | 2021-11-17 | 株式会社オクト | 殺菌用水溶液の製造方法 |
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JPWO2010004699A1 (ja) | 2008-07-09 | 2011-12-22 | パーフェクトペリオ株式会社 | 口臭抑制剤及びその生成方法 |
US20130168329A1 (en) * | 2010-04-28 | 2013-07-04 | The University Of Queensland | Control of bacterial activity, such as in sewers and wastewater treatment systems |
CA2915210A1 (en) | 2013-06-13 | 2014-12-18 | Research Institute At Nationwide Children's Hospital | Compositions and methods for the treatment of burkholderia infections |
AU2014364772B2 (en) | 2013-12-18 | 2018-07-26 | Nutrition & Biosciences USA 4, Inc. | Cationic poly alpha-1,3-glucan ethers |
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WO2008065734A1 (fr) | 2006-11-27 | 2008-06-05 | Oct Incorporated | Composition aqueuse |
JP2014100700A (ja) | 2012-10-23 | 2014-06-05 | Okuto:Kk | 汚染土壌の除染方法 |
JP2016013520A (ja) | 2014-07-02 | 2016-01-28 | 株式会社オクト | 飲料水 |
JP2017500409A5 (ja) | 2014-12-17 | 2018-01-25 | ||
JP2021165284A (ja) | 2020-01-07 | 2021-10-14 | ロート製薬株式会社 | アクネ菌バイオフィルム破壊組成物 |
JP6966818B1 (ja) | 2021-04-30 | 2021-11-17 | 株式会社オクト | 殺菌用水溶液の製造方法 |
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