JP7397791B2 - 初代細胞の遺伝子編集 - Google Patents

初代細胞の遺伝子編集 Download PDF

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JP7397791B2
JP7397791B2 JP2020524267A JP2020524267A JP7397791B2 JP 7397791 B2 JP7397791 B2 JP 7397791B2 JP 2020524267 A JP2020524267 A JP 2020524267A JP 2020524267 A JP2020524267 A JP 2020524267A JP 7397791 B2 JP7397791 B2 JP 7397791B2
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cells
tcr
nucleotide sequence
gene
cell
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JP2021501581A5 (enExample
JP2021501581A (ja
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マーティン ジャコビー カイル
フランズソフ アレクシス
マンデル－キャッシュマン ステファニー
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Pact Pharma Inc
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JP2020524267A 2017-10-30 2018-10-30 初代細胞の遺伝子編集 Active JP7397791B2 (ja)

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JP2023150855A JP2024009805A (ja) 2017-10-30 2023-09-19 初代細胞の遺伝子編集

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US201762579113P 2017-10-30 2017-10-30
US201762579114P 2017-10-30 2017-10-30
US62/579,113 2017-10-30
US62/579,114 2017-10-30
PCT/US2018/058230 WO2019089610A1 (en) 2017-10-30 2018-10-30 Primary cell gene editing

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JP2021501581A5 JP2021501581A5 (enExample) 2021-12-09
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AU (1) AU2018357861B2 (enExample)
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Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2017248259A1 (en) * 2016-04-07 2018-10-25 Bluebird Bio, Inc. Chimeric antigen receptor T cell compositions
MY200418A (en) 2017-06-15 2023-12-25 Univ California Targeted non-viral dna insertions
SG11202003798TA (en) * 2017-10-27 2020-05-28 Univ California Targeted replacement of endogenous t cell receptors
EP3703712A4 (en) * 2017-10-30 2021-09-22 PACT Pharma, Inc. EDITING PRIMARY CELL GENES
KR20200138334A (ko) 2018-04-02 2020-12-09 팩트 파마, 인크. 펩티드-MHC comPACT
CA3116132A1 (en) * 2018-10-16 2020-04-23 Intellia Therapeutics, Inc. Compositions and methods for immunotherapy
MX2021013218A (es) 2019-05-01 2021-12-10 Pact Pharma Inc Composiciones y metodos para el tratamiento del cancer usando una terapia con celulas t modificadas con tet2.
AU2020325225A1 (en) * 2019-08-05 2022-03-24 Cartherics Pty. Ltd. Immune cells expressing modified cell receptors and methods of making
US12359247B2 (en) 2019-08-26 2025-07-15 Adoc Ssf, Llc Methods of performing GUIDE-Seq on primary human T cells
IL291935A (en) * 2019-10-08 2022-06-01 Pact Pharma Inc Methods for immunotherapy of genetically modified autologous t cells
EP4041293A1 (en) * 2019-10-10 2022-08-17 PACT Pharma, Inc. Method of treating immunotherapy non-responders with an autologous cell therapy
EP4110356A4 (en) * 2020-02-28 2024-03-27 Wisconsin Alumni Research Foundation NON-VIRAL GENERATION OF CHIMERIC ANTIGEN RECEPTOR T CELLS OBTAINED BY GENE EDITING
AU2021261011A1 (en) * 2020-04-24 2022-11-10 Pact Pharma, Inc. Methods of determining gene editing efficiencies in cells
WO2021252898A1 (en) * 2020-06-12 2021-12-16 Pact Pharma, Inc. Compositions and methods of manufacturing autologous t cell therapies
IL300994A (en) * 2020-08-28 2023-04-01 Arsenal Biosciences Inc Transgenic immune cells with priming receptors
TW202233830A (zh) * 2020-10-27 2022-09-01 美商Ｐａｃｔ製藥公司 使用下一代工程化t細胞療法治療癌症之組合物及方法
WO2022204443A1 (en) * 2021-03-24 2022-09-29 Genentech, Inc. Efficient tcr gene editing in t lymphocytes
TW202309272A (zh) * 2021-03-24 2023-03-01 美商建南德克公司 Ｔ淋巴球中之高效ｔｃｒ基因編輯
WO2022242644A1 (zh) * 2021-05-18 2022-11-24 赛斯尔擎生物技术（上海）有限公司 修饰细胞的方法
JP2024523014A (ja) 2021-06-11 2024-06-25 パクト ファーマ インコーポレイテッド 細胞製剤を査定する方法
US20260232734A1 (en) 2023-02-27 2026-08-13 Adaptive Biotechnologies Corporation Therapeutic T-Cell Receptors Targeting KRAS G12D

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895020B2 (en) 2002-04-19 2014-11-25 Washington University Single chain trimers and uses therefor
WO2005017149A1 (en) * 2003-06-03 2005-02-24 Cell Genesys, Inc. Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site
US8992937B2 (en) 2006-08-28 2015-03-31 Washington University Disulfide trap MHC class I molecules and uses therefor
WO2013177533A1 (en) * 2012-05-25 2013-11-28 California Institute Of Technology Expression of secreted and cell-surface polypeptides
AU2014235968B2 (en) * 2013-03-21 2018-05-24 Ospedale San Raffaele Srl Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
KR102546296B1 (ko) 2014-10-31 2023-06-21 더 트러스티스 오브 더 유니버시티 오브 펜실베니아 변형된 t 세포에서 유전자 발현의 변경 및 그의 용도
US20190030071A1 (en) * 2014-11-03 2019-01-31 IMMURES S.r.I. T cell receptors
ES2768984T3 (es) * 2015-10-05 2020-06-24 Prec Biosciences Inc Meganucleasas diseñadas con secuencias de reconocimiento encontradas en el gen de la región constante alfa del receptor de células T humanas
ES2803728T3 (es) * 2015-10-05 2021-01-29 Prec Biosciences Inc Células genéticamente modificadas que comprenden un gen de región constante alfa del receptor de linfocitos T humano modificado
US20190241910A1 (en) 2016-03-11 2019-08-08 Bluebird Bio, Inc. Genome edited immune effector cells
SG11202003798TA (en) 2017-10-27 2020-05-28 Univ California Targeted replacement of endogenous t cell receptors
EP3703712A4 (en) * 2017-10-30 2021-09-22 PACT Pharma, Inc. EDITING PRIMARY CELL GENES

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BLOOD, 2017年8月，Vol.130, No.5，p.606-618，DOI 10.1182/blood-2016-08-732636
Molecular Therapy, 2017年4月，Vol.25, No.4，p.1-13，http://dx.doi.org/10.1016/j.ymthe.2017.02.005
Molecular Therapy: Methods & Clinical Development, 2017年3月，Vol.4，p.192-203，http://dx.doi.org/10.1016/j.omtm.2016.12.008.
Nature, 2017年3月，Vol.543，p.113-117

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